High-resolution fluorescence-guided transcranial ultrasound mapping in the live mouse brain

Understanding the physiological impact of transcranial ultrasound in rodent brains may offer an important preclinical model for human scale magnetic resonance–guided focused ultrasound methods. However, precision tools for high-resolution transcranial ultrasound targeting and real-time in vivo track...

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Veröffentlicht in:Science advances 2021-12, Vol.7 (50), p.eabi5464
Hauptverfasser: Estrada, Hector, Robin, Justine, Özbek, Ali, Chen, Zhenyue, Marowsky, Anne, Zhou, Quanyu, Beck, Daniel, le Roy, Beau, Arand, Michael, Shoham, Shy, Razansky, Daniel
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container_issue 50
container_start_page eabi5464
container_title Science advances
container_volume 7
creator Estrada, Hector
Robin, Justine
Özbek, Ali
Chen, Zhenyue
Marowsky, Anne
Zhou, Quanyu
Beck, Daniel
le Roy, Beau
Arand, Michael
Shoham, Shy
Razansky, Daniel
description Understanding the physiological impact of transcranial ultrasound in rodent brains may offer an important preclinical model for human scale magnetic resonance–guided focused ultrasound methods. However, precision tools for high-resolution transcranial ultrasound targeting and real-time in vivo tracking of its effects at the mouse brain scale are currently lacking. We report a versatile bidirectional hybrid fluorescence-ultrasound (FLUS) system incorporating a 0.35-mm precision spherical-phased array ultrasound emission with a fiberscope-based wide-field fluorescence imaging. We show how the marriage between cortex-wide functional imaging and targeted ultrasound delivery can be used to transcranially map previously undocumented localized fluorescence events caused by reversible thermal processes and perform high-speed large-scale recording of neural activity induced by focused ultrasound. FLUS thus naturally harnesses the extensive toolbox of fluorescent tags and ultrasound’s localized bioeffects toward visualizing and causally perturbing a plethora of normal and pathophysiological processes in the living murine brain.
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subjects Applied Sciences and Engineering
Medical Physics
Neuroscience
Physical and Materials Sciences
Physics
SciAdv r-articles
title High-resolution fluorescence-guided transcranial ultrasound mapping in the live mouse brain
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