Tangnaikang Alleviates Hyperglycemia and Improves Gut Microbiota in Diabetic Mice
Dysregulation of gut microbiota contributes to the development of type 2 diabetes. To investigate the antidiabetic effect of Tangnaikang and its regulation of gut microbiota in diabetic KKAy mice, a type 2 diabetes mouse model was established by feeding KKAy mice with a high-fat diet (HFD) for 2 wee...
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| Veröffentlicht in: | Evidence-based complementary and alternative medicine 2021-11, Vol.2021, p.1-11 |
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| creator | Zhang, Liping Wang, Fen He, Hualiang Jiao, Tingting Wu, Lili |
| description | Dysregulation of gut microbiota contributes to the development of type 2 diabetes. To investigate the antidiabetic effect of Tangnaikang and its regulation of gut microbiota in diabetic KKAy mice, a type 2 diabetes mouse model was established by feeding KKAy mice with a high-fat diet (HFD) for 2 weeks. The diabetic KKAy mice were treated with vehicle, Acarbose, or different doses of Tangnaikang once a day for 8 weeks. The fasting plasma glucose (FPG) levels and bodyweights were measured weekly. The fecal and blood samples were collected 8 weeks after treatment. The 16s rRNA sequencing and bioinformatics analysis were conducted to explore the effects of Tangnaikang treatment on the richness, diversity, and relative abundance of gut microbiota. Compared with other treatments, high-dose Tangnaikang (4.68 g/kg) significantly reduced FPG levels while elevating bodyweights in model mice. Compared with saline treatment, different doses of Tangnaikang significantly increased gut microbial species richness and diversity. Linear discriminant analysis effect size identified potential bacterial biomarkers associated with Tangnaikang treatment. Relative abundance analysis revealed that Tangnaikang treatment modulated the abundance of gut bacteria at the class and genus levels, such as Bacilli, Lactobacillus, and Alistipes. The principal component analysis demonstrated that, compared with the samples of the high-dose group, the samples of medium-dose and low-dose groups were closer to those of the model group. Tangnaikang alleviated hyperglycemia and improved the composition and abundance of gut microbiota in diabetic KKAy mice. |
| doi_str_mv | 10.1155/2021/1089176 |
| format | Article |
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To investigate the antidiabetic effect of Tangnaikang and its regulation of gut microbiota in diabetic KKAy mice, a type 2 diabetes mouse model was established by feeding KKAy mice with a high-fat diet (HFD) for 2 weeks. The diabetic KKAy mice were treated with vehicle, Acarbose, or different doses of Tangnaikang once a day for 8 weeks. The fasting plasma glucose (FPG) levels and bodyweights were measured weekly. The fecal and blood samples were collected 8 weeks after treatment. The 16s rRNA sequencing and bioinformatics analysis were conducted to explore the effects of Tangnaikang treatment on the richness, diversity, and relative abundance of gut microbiota. Compared with other treatments, high-dose Tangnaikang (4.68 g/kg) significantly reduced FPG levels while elevating bodyweights in model mice. Compared with saline treatment, different doses of Tangnaikang significantly increased gut microbial species richness and diversity. Linear discriminant analysis effect size identified potential bacterial biomarkers associated with Tangnaikang treatment. Relative abundance analysis revealed that Tangnaikang treatment modulated the abundance of gut bacteria at the class and genus levels, such as Bacilli, Lactobacillus, and Alistipes. The principal component analysis demonstrated that, compared with the samples of the high-dose group, the samples of medium-dose and low-dose groups were closer to those of the model group. Tangnaikang alleviated hyperglycemia and improved the composition and abundance of gut microbiota in diabetic KKAy mice.</description><identifier>ISSN: 1741-427X</identifier><identifier>EISSN: 1741-4288</identifier><identifier>DOI: 10.1155/2021/1089176</identifier><identifier>PMID: 34868327</identifier><language>eng</language><publisher>New York: Hindawi</publisher><subject>Acarbose ; Animal models ; Antidiabetics ; Bacilli ; Bioinformatics ; Chinese medicine ; Diabetes ; Diabetes mellitus (non-insulin dependent) ; Drug dosages ; Feces ; Glucose ; High fat diet ; Hyperglycemia ; Intestinal microflora ; Laboratory animals ; Metabolism ; Microbiota ; Relative abundance ; rRNA 16S ; Species richness ; Statistical analysis ; Taxonomy ; Trends</subject><ispartof>Evidence-based complementary and alternative medicine, 2021-11, Vol.2021, p.1-11</ispartof><rights>Copyright © 2021 Liping Zhang et al.</rights><rights>Copyright © 2021 Liping Zhang et al. This is an open access article distributed under the Creative Commons Attribution License (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. https://creativecommons.org/licenses/by/4.0</rights><rights>Copyright © 2021 Liping Zhang et al. 2021</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c425t-395e8849f062446be31e6a4c2908a5de15f9099b2aeb0871f2aea9f515519e163</citedby><cites>FETCH-LOGICAL-c425t-395e8849f062446be31e6a4c2908a5de15f9099b2aeb0871f2aea9f515519e163</cites><orcidid>0000-0002-5594-5912</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8639253/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8639253/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,53766,53768</link.rule.ids></links><search><contributor>Peng, Weijun</contributor><creatorcontrib>Zhang, Liping</creatorcontrib><creatorcontrib>Wang, Fen</creatorcontrib><creatorcontrib>He, Hualiang</creatorcontrib><creatorcontrib>Jiao, Tingting</creatorcontrib><creatorcontrib>Wu, Lili</creatorcontrib><title>Tangnaikang Alleviates Hyperglycemia and Improves Gut Microbiota in Diabetic Mice</title><title>Evidence-based complementary and alternative medicine</title><description>Dysregulation of gut microbiota contributes to the development of type 2 diabetes. To investigate the antidiabetic effect of Tangnaikang and its regulation of gut microbiota in diabetic KKAy mice, a type 2 diabetes mouse model was established by feeding KKAy mice with a high-fat diet (HFD) for 2 weeks. The diabetic KKAy mice were treated with vehicle, Acarbose, or different doses of Tangnaikang once a day for 8 weeks. The fasting plasma glucose (FPG) levels and bodyweights were measured weekly. The fecal and blood samples were collected 8 weeks after treatment. The 16s rRNA sequencing and bioinformatics analysis were conducted to explore the effects of Tangnaikang treatment on the richness, diversity, and relative abundance of gut microbiota. Compared with other treatments, high-dose Tangnaikang (4.68 g/kg) significantly reduced FPG levels while elevating bodyweights in model mice. Compared with saline treatment, different doses of Tangnaikang significantly increased gut microbial species richness and diversity. Linear discriminant analysis effect size identified potential bacterial biomarkers associated with Tangnaikang treatment. Relative abundance analysis revealed that Tangnaikang treatment modulated the abundance of gut bacteria at the class and genus levels, such as Bacilli, Lactobacillus, and Alistipes. The principal component analysis demonstrated that, compared with the samples of the high-dose group, the samples of medium-dose and low-dose groups were closer to those of the model group. Tangnaikang alleviated hyperglycemia and improved the composition and abundance of gut microbiota in diabetic KKAy mice.</description><subject>Acarbose</subject><subject>Animal models</subject><subject>Antidiabetics</subject><subject>Bacilli</subject><subject>Bioinformatics</subject><subject>Chinese medicine</subject><subject>Diabetes</subject><subject>Diabetes mellitus (non-insulin dependent)</subject><subject>Drug dosages</subject><subject>Feces</subject><subject>Glucose</subject><subject>High fat diet</subject><subject>Hyperglycemia</subject><subject>Intestinal microflora</subject><subject>Laboratory animals</subject><subject>Metabolism</subject><subject>Microbiota</subject><subject>Relative abundance</subject><subject>rRNA 16S</subject><subject>Species richness</subject><subject>Statistical analysis</subject><subject>Taxonomy</subject><subject>Trends</subject><issn>1741-427X</issn><issn>1741-4288</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>RHX</sourceid><sourceid>8G5</sourceid><sourceid>BENPR</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNp9kU1LHEEQhpsQyRr15g8YyCVgVrt7pr8uAVnNKighoOCtqZmt2W2d6Vm7Z1b236eHXYTk4KmKqoe3Pl5CThk9Z0yIC045u2BUG6bkJ3LIVMGmBdf683uunibka4zPlHKjlPpCJnmhpc65OiR_HsAvPbiXFLLLpsGNgx5jdrNdY1g22wpbBxn4RXbbrkO3Sa350Gf3rgpd6boeMuezKwcl9q4ay3hMDmpoIp7s4xF5_HX9MLuZ3v2e384u76ZVwUU_zY1ArQtTU8mLQpaYM5RQVNxQDWKBTNSGGlNywJJqxeqUgKlFOpkZZDI_Ij93uuuhbHFRoe8DNHYdXAthaztw9t-Odyu77DZWy9xwkSeB73uB0L0OGHvbulhh04DHboiWS6pyqqgZZ337D33uhuDTeSMlpVBSsUT92FHpNzEGrN-XYdSOXtnRK7v3KuFnO3zl_ALe3Mf0XzpjkYs</recordid><startdate>20211125</startdate><enddate>20211125</enddate><creator>Zhang, Liping</creator><creator>Wang, Fen</creator><creator>He, Hualiang</creator><creator>Jiao, Tingting</creator><creator>Wu, Lili</creator><general>Hindawi</general><general>Hindawi Limited</general><scope>RHU</scope><scope>RHW</scope><scope>RHX</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7RV</scope><scope>7T5</scope><scope>7TO</scope><scope>7X7</scope><scope>7XB</scope><scope>88G</scope><scope>8AO</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H94</scope><scope>K9.</scope><scope>KB0</scope><scope>M0S</scope><scope>M2M</scope><scope>M2O</scope><scope>MBDVC</scope><scope>NAPCQ</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PSYQQ</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-5594-5912</orcidid></search><sort><creationdate>20211125</creationdate><title>Tangnaikang Alleviates Hyperglycemia and Improves Gut Microbiota in Diabetic Mice</title><author>Zhang, Liping ; 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To investigate the antidiabetic effect of Tangnaikang and its regulation of gut microbiota in diabetic KKAy mice, a type 2 diabetes mouse model was established by feeding KKAy mice with a high-fat diet (HFD) for 2 weeks. The diabetic KKAy mice were treated with vehicle, Acarbose, or different doses of Tangnaikang once a day for 8 weeks. The fasting plasma glucose (FPG) levels and bodyweights were measured weekly. The fecal and blood samples were collected 8 weeks after treatment. The 16s rRNA sequencing and bioinformatics analysis were conducted to explore the effects of Tangnaikang treatment on the richness, diversity, and relative abundance of gut microbiota. Compared with other treatments, high-dose Tangnaikang (4.68 g/kg) significantly reduced FPG levels while elevating bodyweights in model mice. Compared with saline treatment, different doses of Tangnaikang significantly increased gut microbial species richness and diversity. Linear discriminant analysis effect size identified potential bacterial biomarkers associated with Tangnaikang treatment. Relative abundance analysis revealed that Tangnaikang treatment modulated the abundance of gut bacteria at the class and genus levels, such as Bacilli, Lactobacillus, and Alistipes. The principal component analysis demonstrated that, compared with the samples of the high-dose group, the samples of medium-dose and low-dose groups were closer to those of the model group. Tangnaikang alleviated hyperglycemia and improved the composition and abundance of gut microbiota in diabetic KKAy mice.</abstract><cop>New York</cop><pub>Hindawi</pub><pmid>34868327</pmid><doi>10.1155/2021/1089176</doi><tpages>11</tpages><orcidid>https://orcid.org/0000-0002-5594-5912</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Acarbose Animal models Antidiabetics Bacilli Bioinformatics Chinese medicine Diabetes Diabetes mellitus (non-insulin dependent) Drug dosages Feces Glucose High fat diet Hyperglycemia Intestinal microflora Laboratory animals Metabolism Microbiota Relative abundance rRNA 16S Species richness Statistical analysis Taxonomy Trends |
| title | Tangnaikang Alleviates Hyperglycemia and Improves Gut Microbiota in Diabetic Mice |
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