Simultaneous steroids measurement in dogs with hyperadrenocorticism using a column-switching liquid chromatography-tandem mass spectrometry method
We developed an analytical method using an on-line column-switching liquid chromatography with triple quadrupole mass spectrometry (LC/MS/MS) for quantifying multiple steroids in serum. Using the developed method, we evaluated the serum concentration of nine steroids (cortisol, corticosterone, corti...
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| Veröffentlicht in: | Journal of Veterinary Medical Science 2021, Vol.83(11), pp.1634-1642 |
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| creator | SASAKI, Noboru OO, Thandar YASUDA, Yoshikazu ICHISE, Takahiro NAGATA, Noriyuki YOKOYAMA, Nozomu SASAOKA, Kazuyoshi MORISHITA, Keitaro NAKAYAMA, Shouta MM ISHIZUKA, Mayumi NAKAMURA, Kensuke TAKIGUCHI, Mitsuyoshi IKENAKA, Yoshinori |
| description | We developed an analytical method using an on-line column-switching liquid chromatography with triple quadrupole mass spectrometry (LC/MS/MS) for quantifying multiple steroids in serum. Using the developed method, we evaluated the serum concentration of nine steroids (cortisol, corticosterone, cortisone, 11-deoxycortisol, 21-deoxycortisol, deoxycorticosterone, progesterone, 17α-OH-progesterone and aldosterone) in dogs with hyperadrenocorticism (HAC). Serum was mixed with stable isotope internal standards and thereafter purified by the automated column-switching system. The limit of detection ranged 2–16 pg/ml for nine steroids. In the baseline samples, five steroids (cortisol, corticosterone, cortisone, 11-deoxycortisol, and 17α-OH-progesterone) were detected in all dogs. The concentrations of cortisone, 11-deoxycortisol, and 17α-OH-progesterone in dogs with HAC (n=19) were significantly higher those in dogs without HAC (n=15, P |
| doi_str_mv | 10.1292/jvms.21-0406 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_8636878</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2575070328</sourcerecordid><originalsourceid>FETCH-LOGICAL-c583t-fa71573dc5b9696821fb7bfd4af26423aab7ae1d23d32b4e0ab5d9028d2b473f3</originalsourceid><addsrcrecordid>eNpdkc2u1SAUhRuj8R6vznwAEicO7JXfUiYm5sS_5CYO1DGhQFtOSukFes15DZ9Ymp6cRCcQ9vrYi82qqtcI3iEs8PvTo093GNWQwuZJdUCE8ppTIp5WByhQU3PM4E31IqUThBjRRjyvbghllAomDtWfH86vU1azDWsCKdsYnEnAW5XWaL2dM3AzMGFI4LfLIxjPi43KRDsHHWJ22iUP1uTmASigw7T6uU6F1ONWmtzD6gzQYwxe5TBEtYznurgZ64FXqTguVuei2hzPxTWPwbysnvVqSvbVZb-tfn3-9PP4tb7__uXb8eN9rVlLct0rjhgnRrNONKJpMeo73vWGqh43FBOlOq4sMpgYgjtqoeqYERC3ppw46clt9WHvu6ydt0aXWaOa5BKdV_Esg3LyX2V2oxzCo2wb0rS8LQ3eXhrE8LDalKV3Sdtp2n9TYsYZ5JDgDX3zH3oKa5zLeIUSiJIGsqZQ73ZKx5BStP31MQjKLWy5hS0xklvYBT_u-CllNdgrrLZcJrvDLZEIbevl1lXVo4rSzuQv9T66oA</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2591436056</pqid></control><display><type>article</type><title>Simultaneous steroids measurement in dogs with hyperadrenocorticism using a column-switching liquid chromatography-tandem mass spectrometry method</title><source>J-STAGE Free</source><source>PubMed Central Open Access</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><creator>SASAKI, Noboru ; OO, Thandar ; YASUDA, Yoshikazu ; ICHISE, Takahiro ; NAGATA, Noriyuki ; YOKOYAMA, Nozomu ; SASAOKA, Kazuyoshi ; MORISHITA, Keitaro ; NAKAYAMA, Shouta MM ; ISHIZUKA, Mayumi ; NAKAMURA, Kensuke ; TAKIGUCHI, Mitsuyoshi ; IKENAKA, Yoshinori</creator><creatorcontrib>SASAKI, Noboru ; OO, Thandar ; YASUDA, Yoshikazu ; ICHISE, Takahiro ; NAGATA, Noriyuki ; YOKOYAMA, Nozomu ; SASAOKA, Kazuyoshi ; MORISHITA, Keitaro ; NAKAYAMA, Shouta MM ; ISHIZUKA, Mayumi ; NAKAMURA, Kensuke ; TAKIGUCHI, Mitsuyoshi ; IKENAKA, Yoshinori</creatorcontrib><description>We developed an analytical method using an on-line column-switching liquid chromatography with triple quadrupole mass spectrometry (LC/MS/MS) for quantifying multiple steroids in serum. Using the developed method, we evaluated the serum concentration of nine steroids (cortisol, corticosterone, cortisone, 11-deoxycortisol, 21-deoxycortisol, deoxycorticosterone, progesterone, 17α-OH-progesterone and aldosterone) in dogs with hyperadrenocorticism (HAC). Serum was mixed with stable isotope internal standards and thereafter purified by the automated column-switching system. The limit of detection ranged 2–16 pg/ml for nine steroids. In the baseline samples, five steroids (cortisol, corticosterone, cortisone, 11-deoxycortisol, and 17α-OH-progesterone) were detected in all dogs. The concentrations of cortisone, 11-deoxycortisol, and 17α-OH-progesterone in dogs with HAC (n=19) were significantly higher those in dogs without HAC (n=15, P<0.02). After the adrenocorticotropic hormone stimulation test, six steroids (cortisol, corticosterone, cortisone, 11-deoxycortisol, 17α-OH-progesterone, and deoxycorticosterone) were above the limit of quantification in all dogs. Cortisol, corticosterone, cortisone, and deoxycorticosterone concentrations of dogs with HAC were significantly higher than those of dogs without HAC (P<0.02). In addition, 11-deoxycortisol and 17α-OH-progesterone concentration was higher in dogs with HAC than in dogs without HAC (P=0.044 and P=0.048, respectively). The on-line column-switching LC/MS/MS would be feasible for measuring multiple steroids in dog serum. The results suggest that cortisone, 11-deoxycortisol, and 17α-OH-progesterone would be related to HAC. Further studies are warranted to assess the clinical feasibility of steroid profile in dogs with HAC.</description><identifier>ISSN: 0916-7250</identifier><identifier>EISSN: 1347-7439</identifier><identifier>DOI: 10.1292/jvms.21-0406</identifier><identifier>PMID: 34544959</identifier><language>eng</language><publisher>Tokyo: JAPANESE SOCIETY OF VETERINARY SCIENCE</publisher><subject>Adrenocorticotropic hormone ; Aldosterone ; Chromatography ; column-switching ; Corticosterone ; Cortisol ; dog ; Hormones ; hyperadrenocorticism ; Internal Medicine ; Liquid chromatography ; liquid chromatography with triple quadrupole mass spectrometry (LC/MS/MS) ; Mass spectrometry ; Mass spectroscopy ; Progesterone ; Scientific imaging ; Steroid hormones ; steroid profile ; Steroids</subject><ispartof>Journal of Veterinary Medical Science, 2021, Vol.83(11), pp.1634-1642</ispartof><rights>2021 by the Japanese Society of Veterinary Science</rights><rights>2021. This work is published under https://creativecommons.org/licenses/by-nc-nd/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2021 The Japanese Society of Veterinary Science 2021</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c583t-fa71573dc5b9696821fb7bfd4af26423aab7ae1d23d32b4e0ab5d9028d2b473f3</citedby><cites>FETCH-LOGICAL-c583t-fa71573dc5b9696821fb7bfd4af26423aab7ae1d23d32b4e0ab5d9028d2b473f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8636878/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8636878/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,1883,4024,27923,27924,27925,53791,53793</link.rule.ids></links><search><creatorcontrib>SASAKI, Noboru</creatorcontrib><creatorcontrib>OO, Thandar</creatorcontrib><creatorcontrib>YASUDA, Yoshikazu</creatorcontrib><creatorcontrib>ICHISE, Takahiro</creatorcontrib><creatorcontrib>NAGATA, Noriyuki</creatorcontrib><creatorcontrib>YOKOYAMA, Nozomu</creatorcontrib><creatorcontrib>SASAOKA, Kazuyoshi</creatorcontrib><creatorcontrib>MORISHITA, Keitaro</creatorcontrib><creatorcontrib>NAKAYAMA, Shouta MM</creatorcontrib><creatorcontrib>ISHIZUKA, Mayumi</creatorcontrib><creatorcontrib>NAKAMURA, Kensuke</creatorcontrib><creatorcontrib>TAKIGUCHI, Mitsuyoshi</creatorcontrib><creatorcontrib>IKENAKA, Yoshinori</creatorcontrib><title>Simultaneous steroids measurement in dogs with hyperadrenocorticism using a column-switching liquid chromatography-tandem mass spectrometry method</title><title>Journal of Veterinary Medical Science</title><addtitle>J. Vet. Med. Sci.</addtitle><description>We developed an analytical method using an on-line column-switching liquid chromatography with triple quadrupole mass spectrometry (LC/MS/MS) for quantifying multiple steroids in serum. Using the developed method, we evaluated the serum concentration of nine steroids (cortisol, corticosterone, cortisone, 11-deoxycortisol, 21-deoxycortisol, deoxycorticosterone, progesterone, 17α-OH-progesterone and aldosterone) in dogs with hyperadrenocorticism (HAC). Serum was mixed with stable isotope internal standards and thereafter purified by the automated column-switching system. The limit of detection ranged 2–16 pg/ml for nine steroids. In the baseline samples, five steroids (cortisol, corticosterone, cortisone, 11-deoxycortisol, and 17α-OH-progesterone) were detected in all dogs. The concentrations of cortisone, 11-deoxycortisol, and 17α-OH-progesterone in dogs with HAC (n=19) were significantly higher those in dogs without HAC (n=15, P<0.02). After the adrenocorticotropic hormone stimulation test, six steroids (cortisol, corticosterone, cortisone, 11-deoxycortisol, 17α-OH-progesterone, and deoxycorticosterone) were above the limit of quantification in all dogs. Cortisol, corticosterone, cortisone, and deoxycorticosterone concentrations of dogs with HAC were significantly higher than those of dogs without HAC (P<0.02). In addition, 11-deoxycortisol and 17α-OH-progesterone concentration was higher in dogs with HAC than in dogs without HAC (P=0.044 and P=0.048, respectively). The on-line column-switching LC/MS/MS would be feasible for measuring multiple steroids in dog serum. The results suggest that cortisone, 11-deoxycortisol, and 17α-OH-progesterone would be related to HAC. Further studies are warranted to assess the clinical feasibility of steroid profile in dogs with HAC.</description><subject>Adrenocorticotropic hormone</subject><subject>Aldosterone</subject><subject>Chromatography</subject><subject>column-switching</subject><subject>Corticosterone</subject><subject>Cortisol</subject><subject>dog</subject><subject>Hormones</subject><subject>hyperadrenocorticism</subject><subject>Internal Medicine</subject><subject>Liquid chromatography</subject><subject>liquid chromatography with triple quadrupole mass spectrometry (LC/MS/MS)</subject><subject>Mass spectrometry</subject><subject>Mass spectroscopy</subject><subject>Progesterone</subject><subject>Scientific imaging</subject><subject>Steroid hormones</subject><subject>steroid profile</subject><subject>Steroids</subject><issn>0916-7250</issn><issn>1347-7439</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><recordid>eNpdkc2u1SAUhRuj8R6vznwAEicO7JXfUiYm5sS_5CYO1DGhQFtOSukFes15DZ9Ymp6cRCcQ9vrYi82qqtcI3iEs8PvTo093GNWQwuZJdUCE8ppTIp5WByhQU3PM4E31IqUThBjRRjyvbghllAomDtWfH86vU1azDWsCKdsYnEnAW5XWaL2dM3AzMGFI4LfLIxjPi43KRDsHHWJ22iUP1uTmASigw7T6uU6F1ONWmtzD6gzQYwxe5TBEtYznurgZ64FXqTguVuei2hzPxTWPwbysnvVqSvbVZb-tfn3-9PP4tb7__uXb8eN9rVlLct0rjhgnRrNONKJpMeo73vWGqh43FBOlOq4sMpgYgjtqoeqYERC3ppw46clt9WHvu6ydt0aXWaOa5BKdV_Esg3LyX2V2oxzCo2wb0rS8LQ3eXhrE8LDalKV3Sdtp2n9TYsYZ5JDgDX3zH3oKa5zLeIUSiJIGsqZQ73ZKx5BStP31MQjKLWy5hS0xklvYBT_u-CllNdgrrLZcJrvDLZEIbevl1lXVo4rSzuQv9T66oA</recordid><startdate>2021</startdate><enddate>2021</enddate><creator>SASAKI, Noboru</creator><creator>OO, Thandar</creator><creator>YASUDA, Yoshikazu</creator><creator>ICHISE, Takahiro</creator><creator>NAGATA, Noriyuki</creator><creator>YOKOYAMA, Nozomu</creator><creator>SASAOKA, Kazuyoshi</creator><creator>MORISHITA, Keitaro</creator><creator>NAKAYAMA, Shouta MM</creator><creator>ISHIZUKA, Mayumi</creator><creator>NAKAMURA, Kensuke</creator><creator>TAKIGUCHI, Mitsuyoshi</creator><creator>IKENAKA, Yoshinori</creator><general>JAPANESE SOCIETY OF VETERINARY SCIENCE</general><general>Japan Science and Technology Agency</general><general>The Japanese Society of Veterinary Science</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7QR</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>2021</creationdate><title>Simultaneous steroids measurement in dogs with hyperadrenocorticism using a column-switching liquid chromatography-tandem mass spectrometry method</title><author>SASAKI, Noboru ; OO, Thandar ; YASUDA, Yoshikazu ; ICHISE, Takahiro ; NAGATA, Noriyuki ; YOKOYAMA, Nozomu ; SASAOKA, Kazuyoshi ; MORISHITA, Keitaro ; NAKAYAMA, Shouta MM ; ISHIZUKA, Mayumi ; NAKAMURA, Kensuke ; TAKIGUCHI, Mitsuyoshi ; IKENAKA, Yoshinori</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c583t-fa71573dc5b9696821fb7bfd4af26423aab7ae1d23d32b4e0ab5d9028d2b473f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Adrenocorticotropic hormone</topic><topic>Aldosterone</topic><topic>Chromatography</topic><topic>column-switching</topic><topic>Corticosterone</topic><topic>Cortisol</topic><topic>dog</topic><topic>Hormones</topic><topic>hyperadrenocorticism</topic><topic>Internal Medicine</topic><topic>Liquid chromatography</topic><topic>liquid chromatography with triple quadrupole mass spectrometry (LC/MS/MS)</topic><topic>Mass spectrometry</topic><topic>Mass spectroscopy</topic><topic>Progesterone</topic><topic>Scientific imaging</topic><topic>Steroid hormones</topic><topic>steroid profile</topic><topic>Steroids</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>SASAKI, Noboru</creatorcontrib><creatorcontrib>OO, Thandar</creatorcontrib><creatorcontrib>YASUDA, Yoshikazu</creatorcontrib><creatorcontrib>ICHISE, Takahiro</creatorcontrib><creatorcontrib>NAGATA, Noriyuki</creatorcontrib><creatorcontrib>YOKOYAMA, Nozomu</creatorcontrib><creatorcontrib>SASAOKA, Kazuyoshi</creatorcontrib><creatorcontrib>MORISHITA, Keitaro</creatorcontrib><creatorcontrib>NAKAYAMA, Shouta MM</creatorcontrib><creatorcontrib>ISHIZUKA, Mayumi</creatorcontrib><creatorcontrib>NAKAMURA, Kensuke</creatorcontrib><creatorcontrib>TAKIGUCHI, Mitsuyoshi</creatorcontrib><creatorcontrib>IKENAKA, Yoshinori</creatorcontrib><collection>CrossRef</collection><collection>Chemoreception Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of Veterinary Medical Science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>SASAKI, Noboru</au><au>OO, Thandar</au><au>YASUDA, Yoshikazu</au><au>ICHISE, Takahiro</au><au>NAGATA, Noriyuki</au><au>YOKOYAMA, Nozomu</au><au>SASAOKA, Kazuyoshi</au><au>MORISHITA, Keitaro</au><au>NAKAYAMA, Shouta MM</au><au>ISHIZUKA, Mayumi</au><au>NAKAMURA, Kensuke</au><au>TAKIGUCHI, Mitsuyoshi</au><au>IKENAKA, Yoshinori</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Simultaneous steroids measurement in dogs with hyperadrenocorticism using a column-switching liquid chromatography-tandem mass spectrometry method</atitle><jtitle>Journal of Veterinary Medical Science</jtitle><addtitle>J. Vet. Med. Sci.</addtitle><date>2021</date><risdate>2021</risdate><volume>83</volume><issue>11</issue><spage>1634</spage><epage>1642</epage><pages>1634-1642</pages><artnum>21-0406</artnum><issn>0916-7250</issn><eissn>1347-7439</eissn><abstract>We developed an analytical method using an on-line column-switching liquid chromatography with triple quadrupole mass spectrometry (LC/MS/MS) for quantifying multiple steroids in serum. Using the developed method, we evaluated the serum concentration of nine steroids (cortisol, corticosterone, cortisone, 11-deoxycortisol, 21-deoxycortisol, deoxycorticosterone, progesterone, 17α-OH-progesterone and aldosterone) in dogs with hyperadrenocorticism (HAC). Serum was mixed with stable isotope internal standards and thereafter purified by the automated column-switching system. The limit of detection ranged 2–16 pg/ml for nine steroids. In the baseline samples, five steroids (cortisol, corticosterone, cortisone, 11-deoxycortisol, and 17α-OH-progesterone) were detected in all dogs. The concentrations of cortisone, 11-deoxycortisol, and 17α-OH-progesterone in dogs with HAC (n=19) were significantly higher those in dogs without HAC (n=15, P<0.02). After the adrenocorticotropic hormone stimulation test, six steroids (cortisol, corticosterone, cortisone, 11-deoxycortisol, 17α-OH-progesterone, and deoxycorticosterone) were above the limit of quantification in all dogs. Cortisol, corticosterone, cortisone, and deoxycorticosterone concentrations of dogs with HAC were significantly higher than those of dogs without HAC (P<0.02). In addition, 11-deoxycortisol and 17α-OH-progesterone concentration was higher in dogs with HAC than in dogs without HAC (P=0.044 and P=0.048, respectively). The on-line column-switching LC/MS/MS would be feasible for measuring multiple steroids in dog serum. The results suggest that cortisone, 11-deoxycortisol, and 17α-OH-progesterone would be related to HAC. Further studies are warranted to assess the clinical feasibility of steroid profile in dogs with HAC.</abstract><cop>Tokyo</cop><pub>JAPANESE SOCIETY OF VETERINARY SCIENCE</pub><pmid>34544959</pmid><doi>10.1292/jvms.21-0406</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Adrenocorticotropic hormone Aldosterone Chromatography column-switching Corticosterone Cortisol dog Hormones hyperadrenocorticism Internal Medicine Liquid chromatography liquid chromatography with triple quadrupole mass spectrometry (LC/MS/MS) Mass spectrometry Mass spectroscopy Progesterone Scientific imaging Steroid hormones steroid profile Steroids |
| title | Simultaneous steroids measurement in dogs with hyperadrenocorticism using a column-switching liquid chromatography-tandem mass spectrometry method |
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