Diminished amplification of SARS-CoV-2 ORF1ab in a commercial dual-target qRT-PCR diagnostic assay

•SARS-CoV-2 variants can result in PCR target failure shown by CT-value discrepancy.•Multiple targets are key to provide and maintain reliable molecular diagnostics.•Genomic SARS-CoV-2 surveillance must also include primer and probe targeting areas.•Public release of primer/probe sequences are cruci...

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Veröffentlicht in:	Journal of virological methods 2022-02, Vol.300, p.114397-114397, Article 114397
Hauptverfasser:	Popping, Stephanie, Molenkamp, Richard, Weigel, Joachim D., Mutsaers, Pim G.N.J., Voermans, Jolanda C., van Boheemen, Sander, Shamier, Marc C., Sikkema, Reina S., Nieuwenhuijse, David F., Oude Munnink, Bas B., Koopmans, Marion P.G., van Kampen, Jeroen J.A.
Format:	Artikel
Sprache:	eng
Schlagworte:	Cobas COVID-19 Diagnostic Tests, Routine Dual-target assay Humans Molecular diagnostics SARS-CoV-2 ORF1ab mutations PCR amplification Reverse Transcriptase Polymerase Chain Reaction RNA, Viral SARS-CoV-2 Sensitivity and Specificity Short Communication
Online-Zugang:	Volltext
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