Anti-breast cancer synthetic peptides derived from the Anabastestudineus skin mucus fractions
Previous study has shown the antimicrobial activities of mucus protein extracted from Anabas testudineus . In this study, we are interested in characterizing the anticancer activity of the A. testudineus antimicrobial peptides (AMPs). The mucus was extracted, fractioned, and subjected to antibacteri...
Gespeichert in:
| Veröffentlicht in: | Scientific reports 2021-11, Vol.11 (1) |
|---|---|
| Hauptverfasser: | , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | |
|---|---|
| container_issue | 1 |
| container_start_page | |
| container_title | Scientific reports |
| container_volume | 11 |
| creator | Najm, Ahmed Abdul Kareem Azfaralariff, Ahmad Dyari, Herryawan Ryadi Eziwar Othman, Babul Airianah Shahid, Muhammad Khalili, Nahid Law, Douglas Syed Alwi, Sharifah Sakinah Fazry, Shazrul |
| description | Previous study has shown the antimicrobial activities of mucus protein extracted from
Anabas
testudineus
. In this study, we are interested in characterizing the anticancer activity of the
A.
testudineus
antimicrobial peptides (AMPs). The mucus was extracted, fractioned, and subjected to antibacterial activity testing to confirm the fish's AMPs production. The cytotoxic activity of each fraction was also identified. Fraction 2 (F2), which shows toxicity against MCF7 and MDA-MB-231 were sent for peptide sequencing to identify the bioactive peptide. The two peptides were then synthetically produced and subjected to cytotoxic assay to prove their efficacy against cancer cell lines. The IC
50
for AtMP1 against MCF7 and MDA-MB-231 were 8.25 ± 0.14 μg/ml and 9.35 ± 0.25 μg/ml respectively, while for AtMP2 it is 5.89 ± 0.14 μg/ml and 6.97 ± 0.24 μg/ml respectively. AtMP1 and AtMP2 treatment for 48 h induced breast cancer cell cycle arrest and apoptosis by upregulating the p53, which lead to upregulate pro-apoptotic BAX gene and downregulate the anti-apoptotic BCL-2 gene, consequently, trigger the activation of the caspase-3. This interaction was supported by docking analysis (QuickDBD, HPEPDOCK, and ZDOCK) and immunoprecipitation. This study provided new prospects in the development of highly effective and selective cancer therapeutics based on antimicrobial peptides. |
| doi_str_mv | 10.1038/s41598-021-02007-6 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_8632885</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2604656538</sourcerecordid><originalsourceid>FETCH-LOGICAL-p212t-a52b8a9b4e62b5df672215332d7180b7165ca7b88c5511af4b8f0ff670dce1413</originalsourceid><addsrcrecordid>eNpVUU1LAzEQDYLYov0DngKeV_O92YtQil9Q8KJHCUk226a22TXJFvrvjbYgDgwzzHvzmOEBcI3RLUZU3iWGeSMrRHBJhOpKnIEpQYxXhBIyAbOUNqgEJw3DzQWYUCaZrEkzBR_zkH1lotMpQ6uDdRGmQ8hrl72Fgxuyb12CrYt-71rYxX4HCwjnQZuy4lIeWx_cmGD69AHuRlvaLmqbfR_SFTjv9Da52alegvfHh7fFc7V8fXpZzJfVQDDJlebESN0Y5gQxvO1ETQjmlJK2xhKZGgtudW2ktJxjrDtmZIe6QkOtdZhhegnuj7rDaHauDEOOequG6Hc6HlSvvfqPBL9Wq36vpKBESl4Ebk4Csf8ay1dq048xlJsVEYgJLjiVhUWPrFSUw8rFPxZG6scKdbRCFSvUrxVK0G8qbn7Q</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2604656538</pqid></control><display><type>article</type><title>Anti-breast cancer synthetic peptides derived from the Anabastestudineus skin mucus fractions</title><source>Open Access: PubMed Central</source><source>Open Access: Nature Open Access</source><source>Full-Text Journals in Chemistry (Open access)</source><source>Springer_OA刊</source><source>DOAJ Directory of Open Access Journals</source><source>Alma/SFX Local Collection</source><source>EZB Electronic Journals Library</source><creator>Najm, Ahmed Abdul Kareem ; Azfaralariff, Ahmad ; Dyari, Herryawan Ryadi Eziwar ; Othman, Babul Airianah ; Shahid, Muhammad ; Khalili, Nahid ; Law, Douglas ; Syed Alwi, Sharifah Sakinah ; Fazry, Shazrul</creator><creatorcontrib>Najm, Ahmed Abdul Kareem ; Azfaralariff, Ahmad ; Dyari, Herryawan Ryadi Eziwar ; Othman, Babul Airianah ; Shahid, Muhammad ; Khalili, Nahid ; Law, Douglas ; Syed Alwi, Sharifah Sakinah ; Fazry, Shazrul</creatorcontrib><description>Previous study has shown the antimicrobial activities of mucus protein extracted from
Anabas
testudineus
. In this study, we are interested in characterizing the anticancer activity of the
A.
testudineus
antimicrobial peptides (AMPs). The mucus was extracted, fractioned, and subjected to antibacterial activity testing to confirm the fish's AMPs production. The cytotoxic activity of each fraction was also identified. Fraction 2 (F2), which shows toxicity against MCF7 and MDA-MB-231 were sent for peptide sequencing to identify the bioactive peptide. The two peptides were then synthetically produced and subjected to cytotoxic assay to prove their efficacy against cancer cell lines. The IC
50
for AtMP1 against MCF7 and MDA-MB-231 were 8.25 ± 0.14 μg/ml and 9.35 ± 0.25 μg/ml respectively, while for AtMP2 it is 5.89 ± 0.14 μg/ml and 6.97 ± 0.24 μg/ml respectively. AtMP1 and AtMP2 treatment for 48 h induced breast cancer cell cycle arrest and apoptosis by upregulating the p53, which lead to upregulate pro-apoptotic BAX gene and downregulate the anti-apoptotic BCL-2 gene, consequently, trigger the activation of the caspase-3. This interaction was supported by docking analysis (QuickDBD, HPEPDOCK, and ZDOCK) and immunoprecipitation. This study provided new prospects in the development of highly effective and selective cancer therapeutics based on antimicrobial peptides.</description><identifier>EISSN: 2045-2322</identifier><identifier>DOI: 10.1038/s41598-021-02007-6</identifier><identifier>PMID: 34848729</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>631/154 ; 631/337 ; 631/67 ; 631/80 ; Antibacterial activity ; Antimicrobial peptides ; Antitumor activity ; Apoptosis ; BAX gene ; Bcl-2 protein ; Breast cancer ; Caspase-3 ; Cell cycle ; Cytotoxicity ; Humanities and Social Sciences ; Immunoprecipitation ; Mucus ; multidisciplinary ; p53 Protein ; Peptides ; Science ; Science (multidisciplinary) ; Synthetic peptides ; Tumor cell lines</subject><ispartof>Scientific reports, 2021-11, Vol.11 (1)</ispartof><rights>The Author(s) 2021</rights><rights>The Author(s) 2021. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-p212t-a52b8a9b4e62b5df672215332d7180b7165ca7b88c5511af4b8f0ff670dce1413</cites><orcidid>0000-0002-7072-8609</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8632885/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8632885/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,27901,27902,41096,42165,51551,53766,53768</link.rule.ids></links><search><creatorcontrib>Najm, Ahmed Abdul Kareem</creatorcontrib><creatorcontrib>Azfaralariff, Ahmad</creatorcontrib><creatorcontrib>Dyari, Herryawan Ryadi Eziwar</creatorcontrib><creatorcontrib>Othman, Babul Airianah</creatorcontrib><creatorcontrib>Shahid, Muhammad</creatorcontrib><creatorcontrib>Khalili, Nahid</creatorcontrib><creatorcontrib>Law, Douglas</creatorcontrib><creatorcontrib>Syed Alwi, Sharifah Sakinah</creatorcontrib><creatorcontrib>Fazry, Shazrul</creatorcontrib><title>Anti-breast cancer synthetic peptides derived from the Anabastestudineus skin mucus fractions</title><title>Scientific reports</title><addtitle>Sci Rep</addtitle><description>Previous study has shown the antimicrobial activities of mucus protein extracted from
Anabas
testudineus
. In this study, we are interested in characterizing the anticancer activity of the
A.
testudineus
antimicrobial peptides (AMPs). The mucus was extracted, fractioned, and subjected to antibacterial activity testing to confirm the fish's AMPs production. The cytotoxic activity of each fraction was also identified. Fraction 2 (F2), which shows toxicity against MCF7 and MDA-MB-231 were sent for peptide sequencing to identify the bioactive peptide. The two peptides were then synthetically produced and subjected to cytotoxic assay to prove their efficacy against cancer cell lines. The IC
50
for AtMP1 against MCF7 and MDA-MB-231 were 8.25 ± 0.14 μg/ml and 9.35 ± 0.25 μg/ml respectively, while for AtMP2 it is 5.89 ± 0.14 μg/ml and 6.97 ± 0.24 μg/ml respectively. AtMP1 and AtMP2 treatment for 48 h induced breast cancer cell cycle arrest and apoptosis by upregulating the p53, which lead to upregulate pro-apoptotic BAX gene and downregulate the anti-apoptotic BCL-2 gene, consequently, trigger the activation of the caspase-3. This interaction was supported by docking analysis (QuickDBD, HPEPDOCK, and ZDOCK) and immunoprecipitation. This study provided new prospects in the development of highly effective and selective cancer therapeutics based on antimicrobial peptides.</description><subject>631/154</subject><subject>631/337</subject><subject>631/67</subject><subject>631/80</subject><subject>Antibacterial activity</subject><subject>Antimicrobial peptides</subject><subject>Antitumor activity</subject><subject>Apoptosis</subject><subject>BAX gene</subject><subject>Bcl-2 protein</subject><subject>Breast cancer</subject><subject>Caspase-3</subject><subject>Cell cycle</subject><subject>Cytotoxicity</subject><subject>Humanities and Social Sciences</subject><subject>Immunoprecipitation</subject><subject>Mucus</subject><subject>multidisciplinary</subject><subject>p53 Protein</subject><subject>Peptides</subject><subject>Science</subject><subject>Science (multidisciplinary)</subject><subject>Synthetic peptides</subject><subject>Tumor cell lines</subject><issn>2045-2322</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>BENPR</sourceid><recordid>eNpVUU1LAzEQDYLYov0DngKeV_O92YtQil9Q8KJHCUk226a22TXJFvrvjbYgDgwzzHvzmOEBcI3RLUZU3iWGeSMrRHBJhOpKnIEpQYxXhBIyAbOUNqgEJw3DzQWYUCaZrEkzBR_zkH1lotMpQ6uDdRGmQ8hrl72Fgxuyb12CrYt-71rYxX4HCwjnQZuy4lIeWx_cmGD69AHuRlvaLmqbfR_SFTjv9Da52alegvfHh7fFc7V8fXpZzJfVQDDJlebESN0Y5gQxvO1ETQjmlJK2xhKZGgtudW2ktJxjrDtmZIe6QkOtdZhhegnuj7rDaHauDEOOequG6Hc6HlSvvfqPBL9Wq36vpKBESl4Ebk4Csf8ay1dq048xlJsVEYgJLjiVhUWPrFSUw8rFPxZG6scKdbRCFSvUrxVK0G8qbn7Q</recordid><startdate>20211130</startdate><enddate>20211130</enddate><creator>Najm, Ahmed Abdul Kareem</creator><creator>Azfaralariff, Ahmad</creator><creator>Dyari, Herryawan Ryadi Eziwar</creator><creator>Othman, Babul Airianah</creator><creator>Shahid, Muhammad</creator><creator>Khalili, Nahid</creator><creator>Law, Douglas</creator><creator>Syed Alwi, Sharifah Sakinah</creator><creator>Fazry, Shazrul</creator><general>Nature Publishing Group UK</general><general>Nature Publishing Group</general><scope>C6C</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-7072-8609</orcidid></search><sort><creationdate>20211130</creationdate><title>Anti-breast cancer synthetic peptides derived from the Anabastestudineus skin mucus fractions</title><author>Najm, Ahmed Abdul Kareem ; Azfaralariff, Ahmad ; Dyari, Herryawan Ryadi Eziwar ; Othman, Babul Airianah ; Shahid, Muhammad ; Khalili, Nahid ; Law, Douglas ; Syed Alwi, Sharifah Sakinah ; Fazry, Shazrul</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p212t-a52b8a9b4e62b5df672215332d7180b7165ca7b88c5511af4b8f0ff670dce1413</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>631/154</topic><topic>631/337</topic><topic>631/67</topic><topic>631/80</topic><topic>Antibacterial activity</topic><topic>Antimicrobial peptides</topic><topic>Antitumor activity</topic><topic>Apoptosis</topic><topic>BAX gene</topic><topic>Bcl-2 protein</topic><topic>Breast cancer</topic><topic>Caspase-3</topic><topic>Cell cycle</topic><topic>Cytotoxicity</topic><topic>Humanities and Social Sciences</topic><topic>Immunoprecipitation</topic><topic>Mucus</topic><topic>multidisciplinary</topic><topic>p53 Protein</topic><topic>Peptides</topic><topic>Science</topic><topic>Science (multidisciplinary)</topic><topic>Synthetic peptides</topic><topic>Tumor cell lines</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Najm, Ahmed Abdul Kareem</creatorcontrib><creatorcontrib>Azfaralariff, Ahmad</creatorcontrib><creatorcontrib>Dyari, Herryawan Ryadi Eziwar</creatorcontrib><creatorcontrib>Othman, Babul Airianah</creatorcontrib><creatorcontrib>Shahid, Muhammad</creatorcontrib><creatorcontrib>Khalili, Nahid</creatorcontrib><creatorcontrib>Law, Douglas</creatorcontrib><creatorcontrib>Syed Alwi, Sharifah Sakinah</creatorcontrib><creatorcontrib>Fazry, Shazrul</creatorcontrib><collection>Springer_OA刊</collection><collection>ProQuest Central (Corporate)</collection><collection>ProQuest_Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>AUTh Library subscriptions: ProQuest Central</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biological Sciences</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>ProQuest Science Journals</collection><collection>Biological Science Database</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Scientific reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Najm, Ahmed Abdul Kareem</au><au>Azfaralariff, Ahmad</au><au>Dyari, Herryawan Ryadi Eziwar</au><au>Othman, Babul Airianah</au><au>Shahid, Muhammad</au><au>Khalili, Nahid</au><au>Law, Douglas</au><au>Syed Alwi, Sharifah Sakinah</au><au>Fazry, Shazrul</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Anti-breast cancer synthetic peptides derived from the Anabastestudineus skin mucus fractions</atitle><jtitle>Scientific reports</jtitle><stitle>Sci Rep</stitle><date>2021-11-30</date><risdate>2021</risdate><volume>11</volume><issue>1</issue><eissn>2045-2322</eissn><abstract>Previous study has shown the antimicrobial activities of mucus protein extracted from
Anabas
testudineus
. In this study, we are interested in characterizing the anticancer activity of the
A.
testudineus
antimicrobial peptides (AMPs). The mucus was extracted, fractioned, and subjected to antibacterial activity testing to confirm the fish's AMPs production. The cytotoxic activity of each fraction was also identified. Fraction 2 (F2), which shows toxicity against MCF7 and MDA-MB-231 were sent for peptide sequencing to identify the bioactive peptide. The two peptides were then synthetically produced and subjected to cytotoxic assay to prove their efficacy against cancer cell lines. The IC
50
for AtMP1 against MCF7 and MDA-MB-231 were 8.25 ± 0.14 μg/ml and 9.35 ± 0.25 μg/ml respectively, while for AtMP2 it is 5.89 ± 0.14 μg/ml and 6.97 ± 0.24 μg/ml respectively. AtMP1 and AtMP2 treatment for 48 h induced breast cancer cell cycle arrest and apoptosis by upregulating the p53, which lead to upregulate pro-apoptotic BAX gene and downregulate the anti-apoptotic BCL-2 gene, consequently, trigger the activation of the caspase-3. This interaction was supported by docking analysis (QuickDBD, HPEPDOCK, and ZDOCK) and immunoprecipitation. This study provided new prospects in the development of highly effective and selective cancer therapeutics based on antimicrobial peptides.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>34848729</pmid><doi>10.1038/s41598-021-02007-6</doi><orcidid>https://orcid.org/0000-0002-7072-8609</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | EISSN: 2045-2322 |
| ispartof | Scientific reports, 2021-11, Vol.11 (1) |
| issn | 2045-2322 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_8632885 |
| source | Open Access: PubMed Central; Open Access: Nature Open Access; Full-Text Journals in Chemistry (Open access); Springer_OA刊; DOAJ Directory of Open Access Journals; Alma/SFX Local Collection; EZB Electronic Journals Library |
| subjects | 631/154 631/337 631/67 631/80 Antibacterial activity Antimicrobial peptides Antitumor activity Apoptosis BAX gene Bcl-2 protein Breast cancer Caspase-3 Cell cycle Cytotoxicity Humanities and Social Sciences Immunoprecipitation Mucus multidisciplinary p53 Protein Peptides Science Science (multidisciplinary) Synthetic peptides Tumor cell lines |
| title | Anti-breast cancer synthetic peptides derived from the Anabastestudineus skin mucus fractions |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-29T08%3A24%3A04IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Anti-breast%20cancer%20synthetic%20peptides%20derived%20from%20the%20Anabastestudineus%20skin%20mucus%20fractions&rft.jtitle=Scientific%20reports&rft.au=Najm,%20Ahmed%20Abdul%20Kareem&rft.date=2021-11-30&rft.volume=11&rft.issue=1&rft.eissn=2045-2322&rft_id=info:doi/10.1038/s41598-021-02007-6&rft_dat=%3Cproquest_pubme%3E2604656538%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2604656538&rft_id=info:pmid/34848729&rfr_iscdi=true |