Upregulation of ENDOU in cytotrophoblasts from placenta complicated with preeclampsia and fetal growth restriction
Placental hypoplasia is associated with the pathophysiology of fetal growth restriction and preeclampsia. The placenta consists of differentiated trophoblasts, including cytotrophoblasts, syncytiotrophoblasts, and extravillous trophoblasts. Cytotrophoblasts are thought to have stem-like characteris...
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| Veröffentlicht in: | Journal of Clinical Biochemistry and Nutrition 2021, Vol.69(3), pp.280-285 |
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| creator | Nomoto, Masataka Kotani, Tomomi Miki, Rika Ushida, Takafumi Imai, Kenji Iitani, Yukako Tano, Sho Wang, Jingwen Moriyama, Yoshinori Kobayashi, Tomoko Mimura, Nobuko Iriyama, Takayuki Kikkawa, Fumitaka Kajiyama, Hiroaki |
| description | Placental hypoplasia is associated with the pathophysiology of fetal growth restriction and preeclampsia. The placenta consists of differentiated trophoblasts, including cytotrophoblasts, syncytiotrophoblasts, and extravillous trophoblasts. Cytotrophoblasts are thought to have stem-like characteristics and the ability to differentiate into syncytiotrophoblasts and extravillous trophoblasts. However, it is poorly understood whether isolated cytotrophoblasts derived from hypoplastic placentas have specific features compared with those in normal placentas. This study aimed to determine the features of cytotrophoblasts in hypoplastic placentas. Differentially expressed proteins between isolated cytotrophoblasts from hypoplastic placenta with fetal growth restriction and those from the normal placenta were determined by liquid chromatography-tandem mass spectrometry. Among 6,802 proteins, 1,253 and 2,129 proteins were more than 2-fold upregulated and downregulated, respectively. Among them, ENDOU (endonuclease, poly(U) specific), which has high homology with the coronavirus endoribonuclease nonstructural protein 15 (Nsp15), showed a significantly increased expression in cytotrophoblasts from the placenta with fetal growth restriction related to preeclampsia compared with those in normal control placenta. These results provide insight into the pathological mechanisms of placental hypoplasia and additional information on preeclamptic symptoms in cases of SARS-CoV-2 infected placenta, although further investigation is needed. |
| doi_str_mv | 10.3164/jcbn.21-37 |
| format | Article |
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The placenta consists of differentiated trophoblasts, including cytotrophoblasts, syncytiotrophoblasts, and extravillous trophoblasts. Cytotrophoblasts are thought to have stem-like characteristics and the ability to differentiate into syncytiotrophoblasts and extravillous trophoblasts. However, it is poorly understood whether isolated cytotrophoblasts derived from hypoplastic placentas have specific features compared with those in normal placentas. This study aimed to determine the features of cytotrophoblasts in hypoplastic placentas. Differentially expressed proteins between isolated cytotrophoblasts from hypoplastic placenta with fetal growth restriction and those from the normal placenta were determined by liquid chromatography-tandem mass spectrometry. Among 6,802 proteins, 1,253 and 2,129 proteins were more than 2-fold upregulated and downregulated, respectively. Among them, ENDOU (endonuclease, poly(U) specific), which has high homology with the coronavirus endoribonuclease nonstructural protein 15 (Nsp15), showed a significantly increased expression in cytotrophoblasts from the placenta with fetal growth restriction related to preeclampsia compared with those in normal control placenta. These results provide insight into the pathological mechanisms of placental hypoplasia and additional information on preeclamptic symptoms in cases of SARS-CoV-2 infected placenta, although further investigation is needed.</description><identifier>ISSN: 0912-0009</identifier><identifier>EISSN: 1880-5086</identifier><identifier>DOI: 10.3164/jcbn.21-37</identifier><identifier>PMID: 34857990</identifier><language>eng</language><publisher>Gifu: SOCIETY FOR FREE RADICAL RESEARCH JAPAN</publisher><subject>Coronaviruses ; cytotrophoblast ; Endonuclease ; Fetuses ; Homology ; Hypoplasia ; LC-MS/MS ; Liquid chromatography ; Mass spectrometry ; Mass spectroscopy ; Original ; Placenta ; Pre-eclampsia ; Preeclampsia ; Proteins ; Severe acute respiratory syndrome coronavirus 2 ; Trophoblasts ; Viral diseases</subject><ispartof>Journal of Clinical Biochemistry and Nutrition, 2021, Vol.69(3), pp.280-285</ispartof><rights>2021 JCBN</rights><rights>2021. This work is published under https://creativecommons.org/licenses/by-nc-nd/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>Copyright © 2021 JCBN 2021</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c489t-14b7b7cc135698620e86dbd2ce22e6970b6aab4b4052f43405bc7c5b9a12dd673</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8611368/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8611368/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,1877,4010,27900,27901,27902,53766,53768</link.rule.ids></links><search><creatorcontrib>Nomoto, Masataka</creatorcontrib><creatorcontrib>Kotani, Tomomi</creatorcontrib><creatorcontrib>Miki, Rika</creatorcontrib><creatorcontrib>Ushida, Takafumi</creatorcontrib><creatorcontrib>Imai, Kenji</creatorcontrib><creatorcontrib>Iitani, Yukako</creatorcontrib><creatorcontrib>Tano, Sho</creatorcontrib><creatorcontrib>Wang, Jingwen</creatorcontrib><creatorcontrib>Moriyama, Yoshinori</creatorcontrib><creatorcontrib>Kobayashi, Tomoko</creatorcontrib><creatorcontrib>Mimura, Nobuko</creatorcontrib><creatorcontrib>Iriyama, Takayuki</creatorcontrib><creatorcontrib>Kikkawa, Fumitaka</creatorcontrib><creatorcontrib>Kajiyama, Hiroaki</creatorcontrib><title>Upregulation of ENDOU in cytotrophoblasts from placenta complicated with preeclampsia and fetal growth restriction</title><title>Journal of Clinical Biochemistry and Nutrition</title><addtitle>J. Clin. Biochem. Nutr.</addtitle><description>Placental hypoplasia is associated with the pathophysiology of fetal growth restriction and preeclampsia. The placenta consists of differentiated trophoblasts, including cytotrophoblasts, syncytiotrophoblasts, and extravillous trophoblasts. Cytotrophoblasts are thought to have stem-like characteristics and the ability to differentiate into syncytiotrophoblasts and extravillous trophoblasts. However, it is poorly understood whether isolated cytotrophoblasts derived from hypoplastic placentas have specific features compared with those in normal placentas. This study aimed to determine the features of cytotrophoblasts in hypoplastic placentas. Differentially expressed proteins between isolated cytotrophoblasts from hypoplastic placenta with fetal growth restriction and those from the normal placenta were determined by liquid chromatography-tandem mass spectrometry. Among 6,802 proteins, 1,253 and 2,129 proteins were more than 2-fold upregulated and downregulated, respectively. Among them, ENDOU (endonuclease, poly(U) specific), which has high homology with the coronavirus endoribonuclease nonstructural protein 15 (Nsp15), showed a significantly increased expression in cytotrophoblasts from the placenta with fetal growth restriction related to preeclampsia compared with those in normal control placenta. These results provide insight into the pathological mechanisms of placental hypoplasia and additional information on preeclamptic symptoms in cases of SARS-CoV-2 infected placenta, although further investigation is needed.</description><subject>Coronaviruses</subject><subject>cytotrophoblast</subject><subject>Endonuclease</subject><subject>Fetuses</subject><subject>Homology</subject><subject>Hypoplasia</subject><subject>LC-MS/MS</subject><subject>Liquid chromatography</subject><subject>Mass spectrometry</subject><subject>Mass spectroscopy</subject><subject>Original</subject><subject>Placenta</subject><subject>Pre-eclampsia</subject><subject>Preeclampsia</subject><subject>Proteins</subject><subject>Severe acute respiratory syndrome coronavirus 2</subject><subject>Trophoblasts</subject><subject>Viral diseases</subject><issn>0912-0009</issn><issn>1880-5086</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><recordid>eNpdkU1v1DAQhi0Eokvhwi-wxAVVSvFXnPiCQKV8SFV76Z6tsePseuXEwXao-u-bsNVKcJk5vI_feceD0HtKLjmV4tPBmvGS0Yo3L9CGti2patLKl2hDFGUVIUSdoTc5HwgRspbiNTrjoq0bpcgGpe2U3G4OUHwccezx9e23uy32I7aPJZYUp300AXLJuE9xwFMA68YC2MZhCt5CcR1-8GWPFx9nAwxT9oBh7HDvCgS8S_FhUZPLJXm7TnmLXvUQsnv33M_R9vv1_dXP6ubux6-rrzeVFa0qFRWmMY21lNdStZIR18rOdMw6xpxUDTESwAgjSM16wZdmbGNro4CyrpMNP0efj77TbAbXrbETBD0lP0B61BG8_lcZ_V7v4h_dSkq5bBeDj88GKf6elwX04LN1IcDo4pw1k0QqpohQC_rhP_QQ5zQu62lWK7r8O5FioS6OlE0x5-T6UxhK9HpKvZ5SM6r5Gv_LET7kAjt3QiEVb4M7olJpvpa_T06S3UPSbuRPieaqiQ</recordid><startdate>2021</startdate><enddate>2021</enddate><creator>Nomoto, Masataka</creator><creator>Kotani, Tomomi</creator><creator>Miki, Rika</creator><creator>Ushida, Takafumi</creator><creator>Imai, Kenji</creator><creator>Iitani, Yukako</creator><creator>Tano, Sho</creator><creator>Wang, Jingwen</creator><creator>Moriyama, Yoshinori</creator><creator>Kobayashi, Tomoko</creator><creator>Mimura, Nobuko</creator><creator>Iriyama, Takayuki</creator><creator>Kikkawa, Fumitaka</creator><creator>Kajiyama, Hiroaki</creator><general>SOCIETY FOR FREE RADICAL RESEARCH JAPAN</general><general>Japan Science and Technology Agency</general><general>the Society for Free Radical Research Japan</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QP</scope><scope>7TK</scope><scope>7U9</scope><scope>C1K</scope><scope>H94</scope><scope>K9.</scope><scope>NAPCQ</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>2021</creationdate><title>Upregulation of ENDOU in cytotrophoblasts from placenta complicated with preeclampsia and fetal growth restriction</title><author>Nomoto, Masataka ; Kotani, Tomomi ; Miki, Rika ; Ushida, Takafumi ; Imai, Kenji ; Iitani, Yukako ; Tano, Sho ; Wang, Jingwen ; Moriyama, Yoshinori ; Kobayashi, Tomoko ; Mimura, Nobuko ; Iriyama, Takayuki ; Kikkawa, Fumitaka ; Kajiyama, Hiroaki</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c489t-14b7b7cc135698620e86dbd2ce22e6970b6aab4b4052f43405bc7c5b9a12dd673</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Coronaviruses</topic><topic>cytotrophoblast</topic><topic>Endonuclease</topic><topic>Fetuses</topic><topic>Homology</topic><topic>Hypoplasia</topic><topic>LC-MS/MS</topic><topic>Liquid chromatography</topic><topic>Mass spectrometry</topic><topic>Mass spectroscopy</topic><topic>Original</topic><topic>Placenta</topic><topic>Pre-eclampsia</topic><topic>Preeclampsia</topic><topic>Proteins</topic><topic>Severe acute respiratory syndrome coronavirus 2</topic><topic>Trophoblasts</topic><topic>Viral diseases</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nomoto, Masataka</creatorcontrib><creatorcontrib>Kotani, Tomomi</creatorcontrib><creatorcontrib>Miki, Rika</creatorcontrib><creatorcontrib>Ushida, Takafumi</creatorcontrib><creatorcontrib>Imai, Kenji</creatorcontrib><creatorcontrib>Iitani, Yukako</creatorcontrib><creatorcontrib>Tano, Sho</creatorcontrib><creatorcontrib>Wang, Jingwen</creatorcontrib><creatorcontrib>Moriyama, Yoshinori</creatorcontrib><creatorcontrib>Kobayashi, Tomoko</creatorcontrib><creatorcontrib>Mimura, Nobuko</creatorcontrib><creatorcontrib>Iriyama, Takayuki</creatorcontrib><creatorcontrib>Kikkawa, Fumitaka</creatorcontrib><creatorcontrib>Kajiyama, Hiroaki</creatorcontrib><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Premium</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of Clinical Biochemistry and Nutrition</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nomoto, Masataka</au><au>Kotani, Tomomi</au><au>Miki, Rika</au><au>Ushida, Takafumi</au><au>Imai, Kenji</au><au>Iitani, Yukako</au><au>Tano, Sho</au><au>Wang, Jingwen</au><au>Moriyama, Yoshinori</au><au>Kobayashi, Tomoko</au><au>Mimura, Nobuko</au><au>Iriyama, Takayuki</au><au>Kikkawa, Fumitaka</au><au>Kajiyama, Hiroaki</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Upregulation of ENDOU in cytotrophoblasts from placenta complicated with preeclampsia and fetal growth restriction</atitle><jtitle>Journal of Clinical Biochemistry and Nutrition</jtitle><addtitle>J. Clin. Biochem. Nutr.</addtitle><date>2021</date><risdate>2021</risdate><volume>69</volume><issue>3</issue><spage>280</spage><epage>285</epage><pages>280-285</pages><artnum>21-37</artnum><issn>0912-0009</issn><eissn>1880-5086</eissn><abstract>Placental hypoplasia is associated with the pathophysiology of fetal growth restriction and preeclampsia. The placenta consists of differentiated trophoblasts, including cytotrophoblasts, syncytiotrophoblasts, and extravillous trophoblasts. Cytotrophoblasts are thought to have stem-like characteristics and the ability to differentiate into syncytiotrophoblasts and extravillous trophoblasts. However, it is poorly understood whether isolated cytotrophoblasts derived from hypoplastic placentas have specific features compared with those in normal placentas. This study aimed to determine the features of cytotrophoblasts in hypoplastic placentas. Differentially expressed proteins between isolated cytotrophoblasts from hypoplastic placenta with fetal growth restriction and those from the normal placenta were determined by liquid chromatography-tandem mass spectrometry. Among 6,802 proteins, 1,253 and 2,129 proteins were more than 2-fold upregulated and downregulated, respectively. Among them, ENDOU (endonuclease, poly(U) specific), which has high homology with the coronavirus endoribonuclease nonstructural protein 15 (Nsp15), showed a significantly increased expression in cytotrophoblasts from the placenta with fetal growth restriction related to preeclampsia compared with those in normal control placenta. These results provide insight into the pathological mechanisms of placental hypoplasia and additional information on preeclamptic symptoms in cases of SARS-CoV-2 infected placenta, although further investigation is needed.</abstract><cop>Gifu</cop><pub>SOCIETY FOR FREE RADICAL RESEARCH JAPAN</pub><pmid>34857990</pmid><doi>10.3164/jcbn.21-37</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Coronaviruses cytotrophoblast Endonuclease Fetuses Homology Hypoplasia LC-MS/MS Liquid chromatography Mass spectrometry Mass spectroscopy Original Placenta Pre-eclampsia Preeclampsia Proteins Severe acute respiratory syndrome coronavirus 2 Trophoblasts Viral diseases |
| title | Upregulation of ENDOU in cytotrophoblasts from placenta complicated with preeclampsia and fetal growth restriction |
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