Acinetobacter geminorum sp. nov., isolated from human throat swabs
Two isolates of a non-fermenting, Gram-negative bacterial strain were cultured from two throat swabs that were taken from a pair of twins during routine microbiological surveillance screening. As these isolates could not be unambiguously identified using routine diagnostic methods, whole genome sequ...
Gespeichert in:
| Veröffentlicht in: | International journal of systematic and evolutionary microbiology 2021-10, Vol.71 (10) |
|---|---|
| Hauptverfasser: | , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | |
|---|---|
| container_issue | 10 |
| container_start_page | |
| container_title | International journal of systematic and evolutionary microbiology |
| container_volume | 71 |
| creator | Wolf, Sophia Barth-Jakschic, Elisabeth Birkle, Karolin Bader, Baris Marschal, Matthias Liese, Jan Peter, Silke Oberhettinger, Philipp |
| description | Two isolates of a non-fermenting, Gram-negative bacterial strain were cultured from two throat swabs that were taken from a pair of twins during routine microbiological surveillance screening. As these isolates could not be unambiguously identified using routine diagnostic methods, whole genome sequencing was performed followed by phylogenetic analysis based on the
gene sequence and by whole genome datasets. The two strains compose a separate branch within the clade formed by the
(ACB) complex with
CIP 70.29
as the most closely related species. The average nucleotide identity compared to all other species of the ACB complex was below 94.2% and digital DNA-DNA hybridization values were less than 60%. Biochemical characteristics confirm affiliation to the ACB complex with some specific phenotypic differences. As a result of the described data, a new
species is introduced, for which the name
sp. nov. is proposed. The type strain is J00019
with a G+C DNA content of 38.8 mol% and it is deposited in the DSMZ Germany (DSM 111094
) and CCUG Sweden (CCUG 74625
). |
| doi_str_mv | 10.1099/ijsem.0.005018 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_8604166</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2581281050</sourcerecordid><originalsourceid>FETCH-LOGICAL-c390t-29ffad455cb3c615d58792696813b66740bac61f1b373a950d090dfbea7be3223</originalsourceid><addsrcrecordid>eNpVkL1PwzAQxS0EoqWwMqKMDCSc7diNFySo-JIqscBsOYnTuorjYjtF_PcEWiqY7nT37t3TD6FzDBkGIa7NKmibQQbAABcHaIxzzlMGND_c94SP0EkIK4BhAHCMRjTnlApCx-jutjKdjq5UVdQ-WWhrOud7m4R1lnRuk10lJrhWRV0njXc2WfZWdUlceqdiEj5UGU7RUaPaoM92dYLeHu5fZ0_p_OXxeXY7TysqIKZENI2qc8aqklYcs5oVU0G44AWmJefTHIYMHDe4pFOqBIMaBNRNqdW01JQQOkE3W991X1pdV7qLXrVy7Y1V_lM6ZeT_TWeWcuE2suCQY84Hg8udgXfvvQ5RWhMq3baq064PkrACkwIPJAdptpVW3oXgdbN_g0F-g5c_4CXILfjh4OJvuL38lzT9AjAmgB4</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2581281050</pqid></control><display><type>article</type><title>Acinetobacter geminorum sp. nov., isolated from human throat swabs</title><source>MEDLINE</source><source>Microbiology Society</source><source>EZB-FREE-00999 freely available EZB journals</source><source>Alma/SFX Local Collection</source><creator>Wolf, Sophia ; Barth-Jakschic, Elisabeth ; Birkle, Karolin ; Bader, Baris ; Marschal, Matthias ; Liese, Jan ; Peter, Silke ; Oberhettinger, Philipp</creator><creatorcontrib>Wolf, Sophia ; Barth-Jakschic, Elisabeth ; Birkle, Karolin ; Bader, Baris ; Marschal, Matthias ; Liese, Jan ; Peter, Silke ; Oberhettinger, Philipp</creatorcontrib><description>Two isolates of a non-fermenting, Gram-negative bacterial strain were cultured from two throat swabs that were taken from a pair of twins during routine microbiological surveillance screening. As these isolates could not be unambiguously identified using routine diagnostic methods, whole genome sequencing was performed followed by phylogenetic analysis based on the
gene sequence and by whole genome datasets. The two strains compose a separate branch within the clade formed by the
(ACB) complex with
CIP 70.29
as the most closely related species. The average nucleotide identity compared to all other species of the ACB complex was below 94.2% and digital DNA-DNA hybridization values were less than 60%. Biochemical characteristics confirm affiliation to the ACB complex with some specific phenotypic differences. As a result of the described data, a new
species is introduced, for which the name
sp. nov. is proposed. The type strain is J00019
with a G+C DNA content of 38.8 mol% and it is deposited in the DSMZ Germany (DSM 111094
) and CCUG Sweden (CCUG 74625
).</description><identifier>ISSN: 1466-5026</identifier><identifier>EISSN: 1466-5034</identifier><identifier>DOI: 10.1099/ijsem.0.005018</identifier><identifier>PMID: 34633923</identifier><language>eng</language><publisher>England: Microbiology Society</publisher><subject>Acinetobacter - classification ; Acinetobacter - isolation & purification ; Bacterial Typing Techniques ; Base Composition ; DNA, Bacterial - genetics ; Genes, Bacterial ; Humans ; New Taxa ; Nucleic Acid Hybridization ; Pharynx - microbiology ; Phylogeny ; RNA, Ribosomal, 16S - genetics ; Sequence Analysis, DNA</subject><ispartof>International journal of systematic and evolutionary microbiology, 2021-10, Vol.71 (10)</ispartof><rights>2021 The Authors 2021</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c390t-29ffad455cb3c615d58792696813b66740bac61f1b373a950d090dfbea7be3223</citedby><cites>FETCH-LOGICAL-c390t-29ffad455cb3c615d58792696813b66740bac61f1b373a950d090dfbea7be3223</cites><orcidid>0000-0002-7712-9238 ; 0000-0003-1717-3517 ; 0000-0002-6279-3259</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,780,784,885,3744,27922,27923</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34633923$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wolf, Sophia</creatorcontrib><creatorcontrib>Barth-Jakschic, Elisabeth</creatorcontrib><creatorcontrib>Birkle, Karolin</creatorcontrib><creatorcontrib>Bader, Baris</creatorcontrib><creatorcontrib>Marschal, Matthias</creatorcontrib><creatorcontrib>Liese, Jan</creatorcontrib><creatorcontrib>Peter, Silke</creatorcontrib><creatorcontrib>Oberhettinger, Philipp</creatorcontrib><title>Acinetobacter geminorum sp. nov., isolated from human throat swabs</title><title>International journal of systematic and evolutionary microbiology</title><addtitle>Int J Syst Evol Microbiol</addtitle><description>Two isolates of a non-fermenting, Gram-negative bacterial strain were cultured from two throat swabs that were taken from a pair of twins during routine microbiological surveillance screening. As these isolates could not be unambiguously identified using routine diagnostic methods, whole genome sequencing was performed followed by phylogenetic analysis based on the
gene sequence and by whole genome datasets. The two strains compose a separate branch within the clade formed by the
(ACB) complex with
CIP 70.29
as the most closely related species. The average nucleotide identity compared to all other species of the ACB complex was below 94.2% and digital DNA-DNA hybridization values were less than 60%. Biochemical characteristics confirm affiliation to the ACB complex with some specific phenotypic differences. As a result of the described data, a new
species is introduced, for which the name
sp. nov. is proposed. The type strain is J00019
with a G+C DNA content of 38.8 mol% and it is deposited in the DSMZ Germany (DSM 111094
) and CCUG Sweden (CCUG 74625
).</description><subject>Acinetobacter - classification</subject><subject>Acinetobacter - isolation & purification</subject><subject>Bacterial Typing Techniques</subject><subject>Base Composition</subject><subject>DNA, Bacterial - genetics</subject><subject>Genes, Bacterial</subject><subject>Humans</subject><subject>New Taxa</subject><subject>Nucleic Acid Hybridization</subject><subject>Pharynx - microbiology</subject><subject>Phylogeny</subject><subject>RNA, Ribosomal, 16S - genetics</subject><subject>Sequence Analysis, DNA</subject><issn>1466-5026</issn><issn>1466-5034</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkL1PwzAQxS0EoqWwMqKMDCSc7diNFySo-JIqscBsOYnTuorjYjtF_PcEWiqY7nT37t3TD6FzDBkGIa7NKmibQQbAABcHaIxzzlMGND_c94SP0EkIK4BhAHCMRjTnlApCx-jutjKdjq5UVdQ-WWhrOud7m4R1lnRuk10lJrhWRV0njXc2WfZWdUlceqdiEj5UGU7RUaPaoM92dYLeHu5fZ0_p_OXxeXY7TysqIKZENI2qc8aqklYcs5oVU0G44AWmJefTHIYMHDe4pFOqBIMaBNRNqdW01JQQOkE3W991X1pdV7qLXrVy7Y1V_lM6ZeT_TWeWcuE2suCQY84Hg8udgXfvvQ5RWhMq3baq064PkrACkwIPJAdptpVW3oXgdbN_g0F-g5c_4CXILfjh4OJvuL38lzT9AjAmgB4</recordid><startdate>202110</startdate><enddate>202110</enddate><creator>Wolf, Sophia</creator><creator>Barth-Jakschic, Elisabeth</creator><creator>Birkle, Karolin</creator><creator>Bader, Baris</creator><creator>Marschal, Matthias</creator><creator>Liese, Jan</creator><creator>Peter, Silke</creator><creator>Oberhettinger, Philipp</creator><general>Microbiology Society</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-7712-9238</orcidid><orcidid>https://orcid.org/0000-0003-1717-3517</orcidid><orcidid>https://orcid.org/0000-0002-6279-3259</orcidid></search><sort><creationdate>202110</creationdate><title>Acinetobacter geminorum sp. nov., isolated from human throat swabs</title><author>Wolf, Sophia ; Barth-Jakschic, Elisabeth ; Birkle, Karolin ; Bader, Baris ; Marschal, Matthias ; Liese, Jan ; Peter, Silke ; Oberhettinger, Philipp</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c390t-29ffad455cb3c615d58792696813b66740bac61f1b373a950d090dfbea7be3223</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Acinetobacter - classification</topic><topic>Acinetobacter - isolation & purification</topic><topic>Bacterial Typing Techniques</topic><topic>Base Composition</topic><topic>DNA, Bacterial - genetics</topic><topic>Genes, Bacterial</topic><topic>Humans</topic><topic>New Taxa</topic><topic>Nucleic Acid Hybridization</topic><topic>Pharynx - microbiology</topic><topic>Phylogeny</topic><topic>RNA, Ribosomal, 16S - genetics</topic><topic>Sequence Analysis, DNA</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wolf, Sophia</creatorcontrib><creatorcontrib>Barth-Jakschic, Elisabeth</creatorcontrib><creatorcontrib>Birkle, Karolin</creatorcontrib><creatorcontrib>Bader, Baris</creatorcontrib><creatorcontrib>Marschal, Matthias</creatorcontrib><creatorcontrib>Liese, Jan</creatorcontrib><creatorcontrib>Peter, Silke</creatorcontrib><creatorcontrib>Oberhettinger, Philipp</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>International journal of systematic and evolutionary microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wolf, Sophia</au><au>Barth-Jakschic, Elisabeth</au><au>Birkle, Karolin</au><au>Bader, Baris</au><au>Marschal, Matthias</au><au>Liese, Jan</au><au>Peter, Silke</au><au>Oberhettinger, Philipp</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Acinetobacter geminorum sp. nov., isolated from human throat swabs</atitle><jtitle>International journal of systematic and evolutionary microbiology</jtitle><addtitle>Int J Syst Evol Microbiol</addtitle><date>2021-10</date><risdate>2021</risdate><volume>71</volume><issue>10</issue><issn>1466-5026</issn><eissn>1466-5034</eissn><abstract>Two isolates of a non-fermenting, Gram-negative bacterial strain were cultured from two throat swabs that were taken from a pair of twins during routine microbiological surveillance screening. As these isolates could not be unambiguously identified using routine diagnostic methods, whole genome sequencing was performed followed by phylogenetic analysis based on the
gene sequence and by whole genome datasets. The two strains compose a separate branch within the clade formed by the
(ACB) complex with
CIP 70.29
as the most closely related species. The average nucleotide identity compared to all other species of the ACB complex was below 94.2% and digital DNA-DNA hybridization values were less than 60%. Biochemical characteristics confirm affiliation to the ACB complex with some specific phenotypic differences. As a result of the described data, a new
species is introduced, for which the name
sp. nov. is proposed. The type strain is J00019
with a G+C DNA content of 38.8 mol% and it is deposited in the DSMZ Germany (DSM 111094
) and CCUG Sweden (CCUG 74625
).</abstract><cop>England</cop><pub>Microbiology Society</pub><pmid>34633923</pmid><doi>10.1099/ijsem.0.005018</doi><orcidid>https://orcid.org/0000-0002-7712-9238</orcidid><orcidid>https://orcid.org/0000-0003-1717-3517</orcidid><orcidid>https://orcid.org/0000-0002-6279-3259</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1466-5026 |
| ispartof | International journal of systematic and evolutionary microbiology, 2021-10, Vol.71 (10) |
| issn | 1466-5026 1466-5034 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_8604166 |
| source | MEDLINE; Microbiology Society; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Acinetobacter - classification Acinetobacter - isolation & purification Bacterial Typing Techniques Base Composition DNA, Bacterial - genetics Genes, Bacterial Humans New Taxa Nucleic Acid Hybridization Pharynx - microbiology Phylogeny RNA, Ribosomal, 16S - genetics Sequence Analysis, DNA |
| title | Acinetobacter geminorum sp. nov., isolated from human throat swabs |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-13T12%3A48%3A53IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Acinetobacter%20geminorum%20sp.%20nov.,%20isolated%20from%20human%20throat%20swabs&rft.jtitle=International%20journal%20of%20systematic%20and%20evolutionary%20microbiology&rft.au=Wolf,%20Sophia&rft.date=2021-10&rft.volume=71&rft.issue=10&rft.issn=1466-5026&rft.eissn=1466-5034&rft_id=info:doi/10.1099/ijsem.0.005018&rft_dat=%3Cproquest_pubme%3E2581281050%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2581281050&rft_id=info:pmid/34633923&rfr_iscdi=true |