Recovery of Memory B-cell Subsets and Persistence of Antibodies in Convalescent COVID-19 Patients
It is essential to examine the longevity of the defensive immune response engendered by severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) infection. We examined the SARS-CoV-2-specific antibody responses and ex vivo memory B-cell subsets in seven groups of individuals with COVID-19 classi...
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| Veröffentlicht in: | The American journal of tropical medicine and hygiene 2021-11, Vol.105 (5), p.1255-1260 |
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| container_title | The American journal of tropical medicine and hygiene |
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| creator | Rajamanickam, Anuradha Kumar, Nathella Pavan Nancy P, Arul Selvaraj, Nandhini Munisankar, Saravanan Renji, Rachel Mariam V, Vijayalakshmi Murhekar, Manoj Thangaraj, Jeromie Wesley Vivian Kumar, Muthusamy Santhosh Kumar, C P Girish Bhatnagar, Tarun Ponnaiah, Manickam Sabarinathan, R Kumar, V Saravana Babu, Subash |
| description | It is essential to examine the longevity of the defensive immune response engendered by severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) infection. We examined the SARS-CoV-2-specific antibody responses and ex vivo memory B-cell subsets in seven groups of individuals with COVID-19 classified based on days since reverse-transcription polymerase chain reaction confirmation of SARS-CoV-2 infection. Our data showed that the levels of IgG and neutralizing antibodies started increasing from days 15 to 30 to days 61 to 90, and plateaued thereafter. The frequencies of naive B cells and atypical memory B cells decreased from days 15 to 30 to days 61 to 90, and plateaued thereafter. In contrast, the frequencies of immature B cells, classical memory B cells, activated memory B cells, and plasma cells increased from days 15 to 30 to days 61 to 90, and plateaued thereafter. Patients with severe COVID-19 exhibited increased frequencies of naive cells, atypical memory B cells, and activated memory B cells, and lower frequencies of immature B cells, central memory B cells, and plasma cells when compared with patients with mild COVID-19. Therefore, our data suggest modifications in memory B-cell subset frequencies and persistence of humoral immunity in convalescent individuals with COVID-19. |
| doi_str_mv | 10.4269/ajtmh.21-0883 |
| format | Article |
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We examined the SARS-CoV-2-specific antibody responses and ex vivo memory B-cell subsets in seven groups of individuals with COVID-19 classified based on days since reverse-transcription polymerase chain reaction confirmation of SARS-CoV-2 infection. Our data showed that the levels of IgG and neutralizing antibodies started increasing from days 15 to 30 to days 61 to 90, and plateaued thereafter. The frequencies of naive B cells and atypical memory B cells decreased from days 15 to 30 to days 61 to 90, and plateaued thereafter. In contrast, the frequencies of immature B cells, classical memory B cells, activated memory B cells, and plasma cells increased from days 15 to 30 to days 61 to 90, and plateaued thereafter. Patients with severe COVID-19 exhibited increased frequencies of naive cells, atypical memory B cells, and activated memory B cells, and lower frequencies of immature B cells, central memory B cells, and plasma cells when compared with patients with mild COVID-19. 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Therefore, our data suggest modifications in memory B-cell subset frequencies and persistence of humoral immunity in convalescent individuals with COVID-19.</description><subject>Acute Disease</subject><subject>Adolescent</subject><subject>Adult</subject><subject>Aged</subject><subject>Antibodies, Neutralizing - blood</subject><subject>Antibodies, Viral - blood</subject><subject>B-Lymphocyte Subsets - immunology</subject><subject>Convalescence</subject><subject>Coronaviruses</subject><subject>COVID-19</subject><subject>COVID-19 - immunology</subject><subject>Cross-Sectional Studies</subject><subject>Female</subject><subject>Humans</subject><subject>Immunity, Humoral</subject><subject>India</subject><subject>Male</subject><subject>Memory B Cells - immunology</subject><subject>Middle Aged</subject><subject>Severe acute respiratory syndrome coronavirus 2</subject><subject>Young Adult</subject><issn>0002-9637</issn><issn>1476-1645</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkUtv1TAQhS0EoreFJVsUiQ0bF7_jbJDKpUClolZQsbUce0x9lditnVyp_56ElgpYzWjm05nHQegVJceCqe6d3U3j9TGjmGjNn6ANFa3CVAn5FG0IIQx3ircH6LDWHSFUM0qfowMupOaciw2y38DlPZS7JofmK4x5yT5gB8PQfJ_7ClNtbPLNJZQa6wTJwQqepCn22UeoTUzNNqe9HaA6SFOzvfhx9hHTrrm0U1wK9QV6FuxQ4eVDPEJXn06vtl_w-cXns-3JOXaCygk7Za1TrQfPZWuVCFoQEbiw3nsigw5BW0Gpd8BFB5qEvndaa8948I4KfoTe38vezP0Ift2l2MHclDjacmeyjebfTorX5mfeGy07xhhdBN4-CJR8O0OdzBjr-gibIM_VMNm2Ledar7Pe_Ifu8lzSct1CdUoxJaVaKHxPuZJrLRAel6HErN6Z394ZRs3q3cK__vuCR_qPWfwXsj6W9g</recordid><startdate>20211101</startdate><enddate>20211101</enddate><creator>Rajamanickam, Anuradha</creator><creator>Kumar, Nathella Pavan</creator><creator>Nancy P, Arul</creator><creator>Selvaraj, Nandhini</creator><creator>Munisankar, Saravanan</creator><creator>Renji, Rachel Mariam</creator><creator>V, Vijayalakshmi</creator><creator>Murhekar, Manoj</creator><creator>Thangaraj, Jeromie Wesley Vivian</creator><creator>Kumar, Muthusamy Santhosh</creator><creator>Kumar, C P Girish</creator><creator>Bhatnagar, Tarun</creator><creator>Ponnaiah, Manickam</creator><creator>Sabarinathan, R</creator><creator>Kumar, V Saravana</creator><creator>Babu, Subash</creator><general>Institute of Tropical Medicine</general><general>The American Society of Tropical Medicine and Hygiene</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20211101</creationdate><title>Recovery of Memory B-cell Subsets and Persistence of Antibodies in Convalescent COVID-19 Patients</title><author>Rajamanickam, Anuradha ; 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We examined the SARS-CoV-2-specific antibody responses and ex vivo memory B-cell subsets in seven groups of individuals with COVID-19 classified based on days since reverse-transcription polymerase chain reaction confirmation of SARS-CoV-2 infection. Our data showed that the levels of IgG and neutralizing antibodies started increasing from days 15 to 30 to days 61 to 90, and plateaued thereafter. The frequencies of naive B cells and atypical memory B cells decreased from days 15 to 30 to days 61 to 90, and plateaued thereafter. In contrast, the frequencies of immature B cells, classical memory B cells, activated memory B cells, and plasma cells increased from days 15 to 30 to days 61 to 90, and plateaued thereafter. Patients with severe COVID-19 exhibited increased frequencies of naive cells, atypical memory B cells, and activated memory B cells, and lower frequencies of immature B cells, central memory B cells, and plasma cells when compared with patients with mild COVID-19. Therefore, our data suggest modifications in memory B-cell subset frequencies and persistence of humoral immunity in convalescent individuals with COVID-19.</abstract><cop>United States</cop><pub>Institute of Tropical Medicine</pub><pmid>34583334</pmid><doi>10.4269/ajtmh.21-0883</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Acute Disease Adolescent Adult Aged Antibodies, Neutralizing - blood Antibodies, Viral - blood B-Lymphocyte Subsets - immunology Convalescence Coronaviruses COVID-19 COVID-19 - immunology Cross-Sectional Studies Female Humans Immunity, Humoral India Male Memory B Cells - immunology Middle Aged Severe acute respiratory syndrome coronavirus 2 Young Adult |
| title | Recovery of Memory B-cell Subsets and Persistence of Antibodies in Convalescent COVID-19 Patients |
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