LncRNA BASP1‐AS1 interacts with YBX1 to regulate Notch transcription and drives the malignancy of melanoma
Melanoma is a fatal skin malignant tumor with a poor prognosis. We found that long noncoding RNA BASP1‐AS1 is essential for the development and prognosis of melanoma. The methylation, RNA sequencing, copy number variation, mutation data, and sample follow‐up information of melanoma from The Cancer G...
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| Veröffentlicht in: | Cancer science 2021-11, Vol.112 (11), p.4526-4542 |
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| creator | Li, YaLing Gao, YaLi Niu, XueLi Tang, MingSui Li, JingYi Song, Bing Guan, XiuHao |
| description | Melanoma is a fatal skin malignant tumor with a poor prognosis. We found that long noncoding RNA BASP1‐AS1 is essential for the development and prognosis of melanoma. The methylation, RNA sequencing, copy number variation, mutation data, and sample follow‐up information of melanoma from The Cancer Genome Atlas (TCGA) were analyzed using weighted gene co‐expression network analysis and 366 samples common to the three omics were selected for multigroup clustering analysis. A four‐gene prognostic model (BASP1‐AS1, LOC100506098, ARHGAP27P1, and LINC01532) was constructed in the TCGA cohort and validated using the GSE65904 series. The expression of BASP1‐AS1 was upregulated in melanoma tissues and various melanoma cell lines. Functionally, the ectopic expression of BASP1‐AS1 promoted cell proliferation, migration, and invasion in both A375 and SK‐MEL‐2 cells. Mechanically, BASP1‐AS1 interacted with YBX1 and recruited it to the promoter of NOTCH3, initiating its transcription process. The activation of the Notch signaling then resulted in the transcription of multiple oncogenes, including c‐MYC, PCNA, and CDK4, which contributed to melanoma progression. Thus, BASP1‐AS1 could act as a potential biomarker for cutaneous malignant melanoma.
We identified a new type of long noncoding RNA (LncRNA) BASP1‐AS1, which can promote melanoma development both in vivo and in vitro. Detailed studies on the molecular mechanism showed that BASP1‐AS1 promoted the proliferation, invasion, and migration of melanoma cells by regulating YBX1. In addition, LncRNA BASP1‐AS1 is a poor prognostic indicator and a potential therapeutic target for melanoma. |
| doi_str_mv | 10.1111/cas.15140 |
| format | Article |
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We identified a new type of long noncoding RNA (LncRNA) BASP1‐AS1, which can promote melanoma development both in vivo and in vitro. Detailed studies on the molecular mechanism showed that BASP1‐AS1 promoted the proliferation, invasion, and migration of melanoma cells by regulating YBX1. In addition, LncRNA BASP1‐AS1 is a poor prognostic indicator and a potential therapeutic target for melanoma.</description><identifier>ISSN: 1347-9032</identifier><identifier>EISSN: 1349-7006</identifier><identifier>DOI: 10.1111/cas.15140</identifier><identifier>PMID: 34533860</identifier><language>eng</language><publisher>England: John Wiley & Sons, Inc</publisher><subject>Animals ; Antibodies ; Apoptosis ; BASP1‐AS1 ; Biomarkers ; Cancer ; Cell growth ; Cell Line, Tumor ; Cell migration ; Cell Movement ; Cell Proliferation ; Clustering ; Copy number ; DNA methylation ; Ectopic expression ; Gene expression ; Gene Silencing ; Genomes ; GTPase-Activating Proteins - metabolism ; Humans ; Male ; Malignancy ; Medical prognosis ; Melanoma ; Melanoma - metabolism ; Melanoma - mortality ; Melanoma - pathology ; Melanoma, Cutaneous Malignant ; Membrane Proteins - genetics ; Membrane Proteins - metabolism ; Mice ; Mice, Inbred BALB C ; Murine pneumonia virus ; Mutation ; Myc protein ; Neoplasm Invasiveness ; Neoplasm Proteins - metabolism ; Neoplasm Transplantation ; Neoplastic Stem Cells ; Nerve Tissue Proteins - genetics ; Nerve Tissue Proteins - metabolism ; NOTCH3 ; Original ; Prognosis ; Proteins ; Proto-Oncogene Proteins c-bcl-2 - metabolism ; Random Allocation ; Receptor, Notch3 - metabolism ; Repressor Proteins - genetics ; Repressor Proteins - metabolism ; RNA, Long Noncoding - metabolism ; signature ; Skin Neoplasms - metabolism ; Skin Neoplasms - mortality ; Skin Neoplasms - pathology ; Software packages ; Survival analysis ; Transcription activation ; Transcription, Genetic ; Tumors ; Up-Regulation ; Y-Box-Binding Protein 1 - metabolism ; YBX1</subject><ispartof>Cancer science, 2021-11, Vol.112 (11), p.4526-4542</ispartof><rights>2021 The Authors. published by John Wiley & Sons Australia, Ltd on behalf of Japanese Cancer Association.</rights><rights>2021 The Authors. Cancer Science published by John Wiley & Sons Australia, Ltd on behalf of Japanese Cancer Association.</rights><rights>2021. This work is published under http://creativecommons.org/licenses/by-nc-nd/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4670-ff75320a4602b8549ab42ae846ea658c31f63bed1fcfbdd1e9f047c7bb13e6403</citedby><cites>FETCH-LOGICAL-c4670-ff75320a4602b8549ab42ae846ea658c31f63bed1fcfbdd1e9f047c7bb13e6403</cites><orcidid>0000-0002-7308-7077</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8586662/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8586662/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,1411,11542,27903,27904,45553,45554,46031,46455,53770,53772</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34533860$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Li, YaLing</creatorcontrib><creatorcontrib>Gao, YaLi</creatorcontrib><creatorcontrib>Niu, XueLi</creatorcontrib><creatorcontrib>Tang, MingSui</creatorcontrib><creatorcontrib>Li, JingYi</creatorcontrib><creatorcontrib>Song, Bing</creatorcontrib><creatorcontrib>Guan, XiuHao</creatorcontrib><title>LncRNA BASP1‐AS1 interacts with YBX1 to regulate Notch transcription and drives the malignancy of melanoma</title><title>Cancer science</title><addtitle>Cancer Sci</addtitle><description>Melanoma is a fatal skin malignant tumor with a poor prognosis. We found that long noncoding RNA BASP1‐AS1 is essential for the development and prognosis of melanoma. The methylation, RNA sequencing, copy number variation, mutation data, and sample follow‐up information of melanoma from The Cancer Genome Atlas (TCGA) were analyzed using weighted gene co‐expression network analysis and 366 samples common to the three omics were selected for multigroup clustering analysis. A four‐gene prognostic model (BASP1‐AS1, LOC100506098, ARHGAP27P1, and LINC01532) was constructed in the TCGA cohort and validated using the GSE65904 series. The expression of BASP1‐AS1 was upregulated in melanoma tissues and various melanoma cell lines. Functionally, the ectopic expression of BASP1‐AS1 promoted cell proliferation, migration, and invasion in both A375 and SK‐MEL‐2 cells. Mechanically, BASP1‐AS1 interacted with YBX1 and recruited it to the promoter of NOTCH3, initiating its transcription process. The activation of the Notch signaling then resulted in the transcription of multiple oncogenes, including c‐MYC, PCNA, and CDK4, which contributed to melanoma progression. Thus, BASP1‐AS1 could act as a potential biomarker for cutaneous malignant melanoma.
We identified a new type of long noncoding RNA (LncRNA) BASP1‐AS1, which can promote melanoma development both in vivo and in vitro. Detailed studies on the molecular mechanism showed that BASP1‐AS1 promoted the proliferation, invasion, and migration of melanoma cells by regulating YBX1. In addition, LncRNA BASP1‐AS1 is a poor prognostic indicator and a potential therapeutic target for melanoma.</description><subject>Animals</subject><subject>Antibodies</subject><subject>Apoptosis</subject><subject>BASP1‐AS1</subject><subject>Biomarkers</subject><subject>Cancer</subject><subject>Cell growth</subject><subject>Cell Line, Tumor</subject><subject>Cell migration</subject><subject>Cell Movement</subject><subject>Cell Proliferation</subject><subject>Clustering</subject><subject>Copy number</subject><subject>DNA methylation</subject><subject>Ectopic expression</subject><subject>Gene expression</subject><subject>Gene Silencing</subject><subject>Genomes</subject><subject>GTPase-Activating Proteins - metabolism</subject><subject>Humans</subject><subject>Male</subject><subject>Malignancy</subject><subject>Medical prognosis</subject><subject>Melanoma</subject><subject>Melanoma - metabolism</subject><subject>Melanoma - mortality</subject><subject>Melanoma - pathology</subject><subject>Melanoma, Cutaneous Malignant</subject><subject>Membrane Proteins - genetics</subject><subject>Membrane Proteins - metabolism</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Murine pneumonia virus</subject><subject>Mutation</subject><subject>Myc protein</subject><subject>Neoplasm Invasiveness</subject><subject>Neoplasm Proteins - metabolism</subject><subject>Neoplasm Transplantation</subject><subject>Neoplastic Stem Cells</subject><subject>Nerve Tissue Proteins - genetics</subject><subject>Nerve Tissue Proteins - metabolism</subject><subject>NOTCH3</subject><subject>Original</subject><subject>Prognosis</subject><subject>Proteins</subject><subject>Proto-Oncogene Proteins c-bcl-2 - metabolism</subject><subject>Random Allocation</subject><subject>Receptor, Notch3 - metabolism</subject><subject>Repressor Proteins - genetics</subject><subject>Repressor Proteins - metabolism</subject><subject>RNA, Long Noncoding - metabolism</subject><subject>signature</subject><subject>Skin Neoplasms - metabolism</subject><subject>Skin Neoplasms - mortality</subject><subject>Skin Neoplasms - pathology</subject><subject>Software packages</subject><subject>Survival analysis</subject><subject>Transcription activation</subject><subject>Transcription, Genetic</subject><subject>Tumors</subject><subject>Up-Regulation</subject><subject>Y-Box-Binding Protein 1 - metabolism</subject><subject>YBX1</subject><issn>1347-9032</issn><issn>1349-7006</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>24P</sourceid><sourceid>WIN</sourceid><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp1kd9qFDEUh4Mo9o9e-AIS8MZeTJtMMpmZG2G7tFpYqlgFvQqZzMluykyyJpmWvfMRfEafxHS3FhU8NzmQjx-_w4fQC0qOaZ4TreIxrSgnj9A-ZbwtakLE4-1eFy1h5R46iPGaECZ4y5-iPcYrxhpB9tGwcPrj5Qyfzq4-0J_ff8yuKLYuQVA6RXxr0wp_Pf1CcfI4wHIaVAJ86ZNe4RSUizrYdbLeYeV63Ad7AxGnFeBRDXbplNMb7A0eYVDOj-oZemLUEOH5_XuIPp-ffZq_Kxbv317MZ4tCc1GTwpi6YiVRXJCyayreqo6XChouQImq0YwawTroqdGm63sKrSG81nXXUQaCE3aI3uxy11M3Qq_B5bKDXAc7qrCRXln594-zK7n0N7KpGiFEmQNe3wcE_22CmORoo4YhnwF-irKsas5akqtl9NU_6LWfgsvnZaoVJeFlRTN1tKN08DEGMA9lKJF3DmV2KLcOM_vyz_YP5G9pGTjZAbd2gM3_k-Q829xG_gLJN6bW</recordid><startdate>202111</startdate><enddate>202111</enddate><creator>Li, YaLing</creator><creator>Gao, YaLi</creator><creator>Niu, XueLi</creator><creator>Tang, MingSui</creator><creator>Li, JingYi</creator><creator>Song, Bing</creator><creator>Guan, XiuHao</creator><general>John Wiley & Sons, Inc</general><general>John Wiley and Sons Inc</general><scope>24P</scope><scope>WIN</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FE</scope><scope>8FH</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-7308-7077</orcidid></search><sort><creationdate>202111</creationdate><title>LncRNA BASP1‐AS1 interacts with YBX1 to regulate Notch transcription and drives the malignancy of melanoma</title><author>Li, YaLing ; Gao, YaLi ; Niu, XueLi ; Tang, MingSui ; Li, JingYi ; Song, Bing ; Guan, XiuHao</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4670-ff75320a4602b8549ab42ae846ea658c31f63bed1fcfbdd1e9f047c7bb13e6403</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Animals</topic><topic>Antibodies</topic><topic>Apoptosis</topic><topic>BASP1‐AS1</topic><topic>Biomarkers</topic><topic>Cancer</topic><topic>Cell growth</topic><topic>Cell Line, Tumor</topic><topic>Cell migration</topic><topic>Cell Movement</topic><topic>Cell Proliferation</topic><topic>Clustering</topic><topic>Copy number</topic><topic>DNA methylation</topic><topic>Ectopic expression</topic><topic>Gene expression</topic><topic>Gene Silencing</topic><topic>Genomes</topic><topic>GTPase-Activating Proteins - metabolism</topic><topic>Humans</topic><topic>Male</topic><topic>Malignancy</topic><topic>Medical prognosis</topic><topic>Melanoma</topic><topic>Melanoma - metabolism</topic><topic>Melanoma - mortality</topic><topic>Melanoma - pathology</topic><topic>Melanoma, Cutaneous Malignant</topic><topic>Membrane Proteins - genetics</topic><topic>Membrane Proteins - metabolism</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Murine pneumonia virus</topic><topic>Mutation</topic><topic>Myc protein</topic><topic>Neoplasm Invasiveness</topic><topic>Neoplasm Proteins - metabolism</topic><topic>Neoplasm Transplantation</topic><topic>Neoplastic Stem Cells</topic><topic>Nerve Tissue Proteins - genetics</topic><topic>Nerve Tissue Proteins - metabolism</topic><topic>NOTCH3</topic><topic>Original</topic><topic>Prognosis</topic><topic>Proteins</topic><topic>Proto-Oncogene Proteins c-bcl-2 - metabolism</topic><topic>Random Allocation</topic><topic>Receptor, Notch3 - metabolism</topic><topic>Repressor Proteins - genetics</topic><topic>Repressor Proteins - metabolism</topic><topic>RNA, Long Noncoding - metabolism</topic><topic>signature</topic><topic>Skin Neoplasms - metabolism</topic><topic>Skin Neoplasms - mortality</topic><topic>Skin Neoplasms - pathology</topic><topic>Software packages</topic><topic>Survival analysis</topic><topic>Transcription activation</topic><topic>Transcription, Genetic</topic><topic>Tumors</topic><topic>Up-Regulation</topic><topic>Y-Box-Binding Protein 1 - metabolism</topic><topic>YBX1</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Li, YaLing</creatorcontrib><creatorcontrib>Gao, YaLi</creatorcontrib><creatorcontrib>Niu, XueLi</creatorcontrib><creatorcontrib>Tang, MingSui</creatorcontrib><creatorcontrib>Li, JingYi</creatorcontrib><creatorcontrib>Song, Bing</creatorcontrib><creatorcontrib>Guan, XiuHao</creatorcontrib><collection>Wiley Online Library Open Access</collection><collection>Wiley-Blackwell Free Backfiles(OpenAccess)</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>ProQuest Biological Science Journals</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Cancer science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, YaLing</au><au>Gao, YaLi</au><au>Niu, XueLi</au><au>Tang, MingSui</au><au>Li, JingYi</au><au>Song, Bing</au><au>Guan, XiuHao</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>LncRNA BASP1‐AS1 interacts with YBX1 to regulate Notch transcription and drives the malignancy of melanoma</atitle><jtitle>Cancer science</jtitle><addtitle>Cancer Sci</addtitle><date>2021-11</date><risdate>2021</risdate><volume>112</volume><issue>11</issue><spage>4526</spage><epage>4542</epage><pages>4526-4542</pages><issn>1347-9032</issn><eissn>1349-7006</eissn><abstract>Melanoma is a fatal skin malignant tumor with a poor prognosis. We found that long noncoding RNA BASP1‐AS1 is essential for the development and prognosis of melanoma. The methylation, RNA sequencing, copy number variation, mutation data, and sample follow‐up information of melanoma from The Cancer Genome Atlas (TCGA) were analyzed using weighted gene co‐expression network analysis and 366 samples common to the three omics were selected for multigroup clustering analysis. A four‐gene prognostic model (BASP1‐AS1, LOC100506098, ARHGAP27P1, and LINC01532) was constructed in the TCGA cohort and validated using the GSE65904 series. The expression of BASP1‐AS1 was upregulated in melanoma tissues and various melanoma cell lines. Functionally, the ectopic expression of BASP1‐AS1 promoted cell proliferation, migration, and invasion in both A375 and SK‐MEL‐2 cells. Mechanically, BASP1‐AS1 interacted with YBX1 and recruited it to the promoter of NOTCH3, initiating its transcription process. The activation of the Notch signaling then resulted in the transcription of multiple oncogenes, including c‐MYC, PCNA, and CDK4, which contributed to melanoma progression. Thus, BASP1‐AS1 could act as a potential biomarker for cutaneous malignant melanoma.
We identified a new type of long noncoding RNA (LncRNA) BASP1‐AS1, which can promote melanoma development both in vivo and in vitro. Detailed studies on the molecular mechanism showed that BASP1‐AS1 promoted the proliferation, invasion, and migration of melanoma cells by regulating YBX1. In addition, LncRNA BASP1‐AS1 is a poor prognostic indicator and a potential therapeutic target for melanoma.</abstract><cop>England</cop><pub>John Wiley & Sons, Inc</pub><pmid>34533860</pmid><doi>10.1111/cas.15140</doi><tpages>0</tpages><orcidid>https://orcid.org/0000-0002-7308-7077</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Antibodies Apoptosis BASP1‐AS1 Biomarkers Cancer Cell growth Cell Line, Tumor Cell migration Cell Movement Cell Proliferation Clustering Copy number DNA methylation Ectopic expression Gene expression Gene Silencing Genomes GTPase-Activating Proteins - metabolism Humans Male Malignancy Medical prognosis Melanoma Melanoma - metabolism Melanoma - mortality Melanoma - pathology Melanoma, Cutaneous Malignant Membrane Proteins - genetics Membrane Proteins - metabolism Mice Mice, Inbred BALB C Murine pneumonia virus Mutation Myc protein Neoplasm Invasiveness Neoplasm Proteins - metabolism Neoplasm Transplantation Neoplastic Stem Cells Nerve Tissue Proteins - genetics Nerve Tissue Proteins - metabolism NOTCH3 Original Prognosis Proteins Proto-Oncogene Proteins c-bcl-2 - metabolism Random Allocation Receptor, Notch3 - metabolism Repressor Proteins - genetics Repressor Proteins - metabolism RNA, Long Noncoding - metabolism signature Skin Neoplasms - metabolism Skin Neoplasms - mortality Skin Neoplasms - pathology Software packages Survival analysis Transcription activation Transcription, Genetic Tumors Up-Regulation Y-Box-Binding Protein 1 - metabolism YBX1 |
| title | LncRNA BASP1‐AS1 interacts with YBX1 to regulate Notch transcription and drives the malignancy of melanoma |
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