Pre-Analytical Modification of Serum miRNAs: Diagnostic Reliability of Serum miRNAs in Hemolytic Diseases
Circulating microRNAs (miRNAs) are useful biomarkers of hemolysis. Since blood cells are the main origins of circulating miRNAs, we evaluated blood cell-related pre-analytical modification of the miRNA signatures during blood drawing and serum processing. The levels of miRNA before and after ex vivo...
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| Veröffentlicht in: | Journal of clinical medicine 2021-10, Vol.10 (21), p.5045 |
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| creator | Takada, Yukichi Shibuta, Tatsuki Hatano, Mayu Sato, Kenichi Koga, Mari Ishibashi, Ayaka Harada, Tetsuhiro Hisatomi, Takashi Shimura, Hanae Fukushima, Noriyasu Leecharoenkiat, Kamonlak Chamnanchanunt, Supat Svasti, Saovaros Fucharoen, Suthat Umemura, Tsukuru |
| description | Circulating microRNAs (miRNAs) are useful biomarkers of hemolysis. Since blood cells are the main origins of circulating miRNAs, we evaluated blood cell-related pre-analytical modification of the miRNA signatures during blood drawing and serum processing. The levels of miRNA before and after ex vivo blood drawing were analyzed with the reverse transcriptase-based polymerase chain reaction method. Furthermore, the changes of miRNA signatures caused by different time-lag between blood drawing and serum preparation by 24 h were evaluated. Finally, we compared the miRNA levels between leftover samples and samples of hemolytic diseases. Blood drawing procedure induced increments of red blood cell (RBC)-related miRNAs (miR-451a, miR-486) about 2-fold. One hour standing of blood samples before serum separation induced almost the same increases in RBC-related miRNAs. To test the clinical usefulness of miR-451a as a biomarker of hemolytic diseases, we analyzed miRNAs of samples from 10 normal subjects, 30 leftover samples in the clinical laboratory, and 20 samples from patients with hemolytic diseases. Serum miR-451a significantly increased in patients with hemolytic anemia more than the levels of pre-analytical modification. In conclusion, the pre-analytical modification of serum miRNAs did not disturb the usefulness of RBC-derived miRNAs as biomarkers of hemolytic diseases. |
| doi_str_mv | 10.3390/jcm10215045 |
| format | Article |
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Since blood cells are the main origins of circulating miRNAs, we evaluated blood cell-related pre-analytical modification of the miRNA signatures during blood drawing and serum processing. The levels of miRNA before and after ex vivo blood drawing were analyzed with the reverse transcriptase-based polymerase chain reaction method. Furthermore, the changes of miRNA signatures caused by different time-lag between blood drawing and serum preparation by 24 h were evaluated. Finally, we compared the miRNA levels between leftover samples and samples of hemolytic diseases. Blood drawing procedure induced increments of red blood cell (RBC)-related miRNAs (miR-451a, miR-486) about 2-fold. One hour standing of blood samples before serum separation induced almost the same increases in RBC-related miRNAs. To test the clinical usefulness of miR-451a as a biomarker of hemolytic diseases, we analyzed miRNAs of samples from 10 normal subjects, 30 leftover samples in the clinical laboratory, and 20 samples from patients with hemolytic diseases. Serum miR-451a significantly increased in patients with hemolytic anemia more than the levels of pre-analytical modification. In conclusion, the pre-analytical modification of serum miRNAs did not disturb the usefulness of RBC-derived miRNAs as biomarkers of hemolytic diseases.</description><identifier>ISSN: 2077-0383</identifier><identifier>EISSN: 2077-0383</identifier><identifier>DOI: 10.3390/jcm10215045</identifier><identifier>PMID: 34768564</identifier><language>eng</language><publisher>Basel: MDPI AG</publisher><subject>Anemia ; Biomarkers ; Blood ; Blood diseases ; Bone marrow ; Clinical medicine ; Electron tubes ; Granulocytes ; Hemoglobin ; Medical laboratories ; Methods ; MicroRNAs ; Potassium</subject><ispartof>Journal of clinical medicine, 2021-10, Vol.10 (21), p.5045</ispartof><rights>2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2021 by the authors. 2021</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c452t-1bdf7d798d9e176d7142bdd2ab1659aad32723517da9827fbef5344890c465893</citedby><cites>FETCH-LOGICAL-c452t-1bdf7d798d9e176d7142bdd2ab1659aad32723517da9827fbef5344890c465893</cites><orcidid>0000-0003-1956-1097 ; 0000-0003-1581-4974</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8584813/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8584813/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids></links><search><creatorcontrib>Takada, Yukichi</creatorcontrib><creatorcontrib>Shibuta, Tatsuki</creatorcontrib><creatorcontrib>Hatano, Mayu</creatorcontrib><creatorcontrib>Sato, Kenichi</creatorcontrib><creatorcontrib>Koga, Mari</creatorcontrib><creatorcontrib>Ishibashi, Ayaka</creatorcontrib><creatorcontrib>Harada, Tetsuhiro</creatorcontrib><creatorcontrib>Hisatomi, Takashi</creatorcontrib><creatorcontrib>Shimura, Hanae</creatorcontrib><creatorcontrib>Fukushima, Noriyasu</creatorcontrib><creatorcontrib>Leecharoenkiat, Kamonlak</creatorcontrib><creatorcontrib>Chamnanchanunt, Supat</creatorcontrib><creatorcontrib>Svasti, Saovaros</creatorcontrib><creatorcontrib>Fucharoen, Suthat</creatorcontrib><creatorcontrib>Umemura, Tsukuru</creatorcontrib><title>Pre-Analytical Modification of Serum miRNAs: Diagnostic Reliability of Serum miRNAs in Hemolytic Diseases</title><title>Journal of clinical medicine</title><description>Circulating microRNAs (miRNAs) are useful biomarkers of hemolysis. Since blood cells are the main origins of circulating miRNAs, we evaluated blood cell-related pre-analytical modification of the miRNA signatures during blood drawing and serum processing. The levels of miRNA before and after ex vivo blood drawing were analyzed with the reverse transcriptase-based polymerase chain reaction method. Furthermore, the changes of miRNA signatures caused by different time-lag between blood drawing and serum preparation by 24 h were evaluated. Finally, we compared the miRNA levels between leftover samples and samples of hemolytic diseases. Blood drawing procedure induced increments of red blood cell (RBC)-related miRNAs (miR-451a, miR-486) about 2-fold. One hour standing of blood samples before serum separation induced almost the same increases in RBC-related miRNAs. To test the clinical usefulness of miR-451a as a biomarker of hemolytic diseases, we analyzed miRNAs of samples from 10 normal subjects, 30 leftover samples in the clinical laboratory, and 20 samples from patients with hemolytic diseases. Serum miR-451a significantly increased in patients with hemolytic anemia more than the levels of pre-analytical modification. In conclusion, the pre-analytical modification of serum miRNAs did not disturb the usefulness of RBC-derived miRNAs as biomarkers of hemolytic diseases.</description><subject>Anemia</subject><subject>Biomarkers</subject><subject>Blood</subject><subject>Blood diseases</subject><subject>Bone marrow</subject><subject>Clinical medicine</subject><subject>Electron tubes</subject><subject>Granulocytes</subject><subject>Hemoglobin</subject><subject>Medical laboratories</subject><subject>Methods</subject><subject>MicroRNAs</subject><subject>Potassium</subject><issn>2077-0383</issn><issn>2077-0383</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><recordid>eNpdkVtLxDAQhYMoKus--QcCvghSzT2pD8KyXsEbqz6HtEk1S9to0gr77627i6jzMgfmm8MwB4B9jI4pzdHJvGwwIpgjxjfALkFSZogquvlL74BxSnM0lFKMYLkNdiiTQnHBdoF_jC6btKZedL40NbwL1leD6nxoYajgk4t9Axs_u5-kU3juzWsb0oDCmau9KXztu8V_DvoWXrsmLD2HneRMcmkPbFWmTm687iPwcnnxPL3Obh-ubqaT26xknHQZLmwlrcyVzR2WwkrMSGEtMQUWPDfGUiIJ5Vhakysiq8JVnDKmclQywVVOR-Bs5fveF42zpWu7aGr9Hn1j4kIH4_XfSevf9Gv41IorpjAdDA7XBjF89C51uvGpdHVtWhf6pAnPJVOCMDygB__Qeejj8M0lJRAVSrCBOlpRZQwpRVf9HIOR_k5R_0qRfgFN_I34</recordid><startdate>20211028</startdate><enddate>20211028</enddate><creator>Takada, Yukichi</creator><creator>Shibuta, Tatsuki</creator><creator>Hatano, Mayu</creator><creator>Sato, Kenichi</creator><creator>Koga, Mari</creator><creator>Ishibashi, Ayaka</creator><creator>Harada, Tetsuhiro</creator><creator>Hisatomi, Takashi</creator><creator>Shimura, Hanae</creator><creator>Fukushima, Noriyasu</creator><creator>Leecharoenkiat, Kamonlak</creator><creator>Chamnanchanunt, Supat</creator><creator>Svasti, Saovaros</creator><creator>Fucharoen, Suthat</creator><creator>Umemura, Tsukuru</creator><general>MDPI AG</general><general>MDPI</general><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>M0S</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0003-1956-1097</orcidid><orcidid>https://orcid.org/0000-0003-1581-4974</orcidid></search><sort><creationdate>20211028</creationdate><title>Pre-Analytical Modification of Serum miRNAs: Diagnostic Reliability of Serum miRNAs in Hemolytic Diseases</title><author>Takada, Yukichi ; 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Since blood cells are the main origins of circulating miRNAs, we evaluated blood cell-related pre-analytical modification of the miRNA signatures during blood drawing and serum processing. The levels of miRNA before and after ex vivo blood drawing were analyzed with the reverse transcriptase-based polymerase chain reaction method. Furthermore, the changes of miRNA signatures caused by different time-lag between blood drawing and serum preparation by 24 h were evaluated. Finally, we compared the miRNA levels between leftover samples and samples of hemolytic diseases. Blood drawing procedure induced increments of red blood cell (RBC)-related miRNAs (miR-451a, miR-486) about 2-fold. One hour standing of blood samples before serum separation induced almost the same increases in RBC-related miRNAs. To test the clinical usefulness of miR-451a as a biomarker of hemolytic diseases, we analyzed miRNAs of samples from 10 normal subjects, 30 leftover samples in the clinical laboratory, and 20 samples from patients with hemolytic diseases. Serum miR-451a significantly increased in patients with hemolytic anemia more than the levels of pre-analytical modification. In conclusion, the pre-analytical modification of serum miRNAs did not disturb the usefulness of RBC-derived miRNAs as biomarkers of hemolytic diseases.</abstract><cop>Basel</cop><pub>MDPI AG</pub><pmid>34768564</pmid><doi>10.3390/jcm10215045</doi><orcidid>https://orcid.org/0000-0003-1956-1097</orcidid><orcidid>https://orcid.org/0000-0003-1581-4974</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Anemia Biomarkers Blood Blood diseases Bone marrow Clinical medicine Electron tubes Granulocytes Hemoglobin Medical laboratories Methods MicroRNAs Potassium |
| title | Pre-Analytical Modification of Serum miRNAs: Diagnostic Reliability of Serum miRNAs in Hemolytic Diseases |
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