Modification of human pericardium by chemical crosslinking
Autologous and allogenic human pericardia used as biomaterials for cardiovascular surgery are traditionally crosslinked with glutaraldehyde. In this work, we have evaluated the resistivity to collagenase digestion and the cytotoxicity of human pericardium crosslinked with various concentrations of g...
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| Veröffentlicht in: | Physiological research 2020-02, Vol.69 (1), p.49-59 |
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| creator | Filová, E Staňková, L Eckhardt, A Svobodová, J Musílková, J Pala, J Hadraba, D Brynda, E Koňařík, M Pirk, J Bačáková, L |
| description | Autologous and allogenic human pericardia used as biomaterials for cardiovascular surgery are traditionally crosslinked with glutaraldehyde. In this work, we have evaluated the resistivity to collagenase digestion and the cytotoxicity of human pericardium crosslinked with various concentrations of glutaraldehyde in comparison with pericardium crosslinked by genipin, nordihydroguaiaretic acid, tannic acid, and in comparison with unmodified pericardium. Crosslinking retained the wavy-like morphology of native pericardium visualized by second harmonic generation microscopy. The collagenase digestion products were analyzed using SDS-PAGE, capillary electrophoresis, and a hydroxyproline assay. Glutaraldehyde and genipin crosslinking protected the native pericardium efficiently against digestion with collagenase III. Only low protection was provided by the other crosslinking agents. The cytotoxicity of crosslinked pericardium was evaluated using xCELLigence by monitoring the viability of porcine valve interstitial cells cultured in eluates from crosslinked pericardium. The highest cell index, reflecting both the number and the shape of the monitored cells was observed in eluates from genipin. Crosslinking pericardium grafts with genipin therefore seems to be a promising alternative procedure to the traditional crosslinking with glutaraldehyde, because it provides similarly high protection against degradation with collagenase, without cytotoxic effects. |
| doi_str_mv | 10.33549/physiolres.934335 |
| format | Article |
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In this work, we have evaluated the resistivity to collagenase digestion and the cytotoxicity of human pericardium crosslinked with various concentrations of glutaraldehyde in comparison with pericardium crosslinked by genipin, nordihydroguaiaretic acid, tannic acid, and in comparison with unmodified pericardium. Crosslinking retained the wavy-like morphology of native pericardium visualized by second harmonic generation microscopy. The collagenase digestion products were analyzed using SDS-PAGE, capillary electrophoresis, and a hydroxyproline assay. Glutaraldehyde and genipin crosslinking protected the native pericardium efficiently against digestion with collagenase III. Only low protection was provided by the other crosslinking agents. The cytotoxicity of crosslinked pericardium was evaluated using xCELLigence by monitoring the viability of porcine valve interstitial cells cultured in eluates from crosslinked pericardium. The highest cell index, reflecting both the number and the shape of the monitored cells was observed in eluates from genipin. Crosslinking pericardium grafts with genipin therefore seems to be a promising alternative procedure to the traditional crosslinking with glutaraldehyde, because it provides similarly high protection against degradation with collagenase, without cytotoxic effects.</description><identifier>ISSN: 0862-8408</identifier><identifier>EISSN: 1802-9973</identifier><identifier>DOI: 10.33549/physiolres.934335</identifier><identifier>PMID: 31852209</identifier><language>eng</language><publisher>Czech Republic: Institute of Physiology</publisher><subject>Acids ; Autografts ; Biocompatible Materials ; Biomaterials ; Biomedical materials ; Calcification ; Capillary electrophoresis ; Collagen ; Collagenase ; Collagenase 3 ; Cross-Linking Reagents ; Cytotoxicity ; Gel electrophoresis ; Genipin ; Glutaral ; Glutaraldehyde ; Humans ; Hydroxyproline ; Interstitial cells ; Iridoids ; Masoprocol ; Nordihydroguaiaretic acid ; Pathogens ; Pericardium ; Pericardium - chemistry ; Sodium lauryl sulfate ; Surgery ; Tannic acid ; Tannins ; Transplants - chemistry</subject><ispartof>Physiological research, 2020-02, Vol.69 (1), p.49-59</ispartof><rights>Copyright Institute of Physiology 2020</rights><rights>2020 Institute of Physiology of the Czech Academy of Sciences, Prague, Czech Republic 2020</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c397t-79a09c3dc972339bb7e01706ab70a23961551392e09ee7ecce92bf1196a22eb3</citedby><cites>FETCH-LOGICAL-c397t-79a09c3dc972339bb7e01706ab70a23961551392e09ee7ecce92bf1196a22eb3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8565964/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8565964/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,864,885,27923,27924,53790,53792</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31852209$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Filová, E</creatorcontrib><creatorcontrib>Staňková, L</creatorcontrib><creatorcontrib>Eckhardt, A</creatorcontrib><creatorcontrib>Svobodová, J</creatorcontrib><creatorcontrib>Musílková, J</creatorcontrib><creatorcontrib>Pala, J</creatorcontrib><creatorcontrib>Hadraba, D</creatorcontrib><creatorcontrib>Brynda, E</creatorcontrib><creatorcontrib>Koňařík, M</creatorcontrib><creatorcontrib>Pirk, J</creatorcontrib><creatorcontrib>Bačáková, L</creatorcontrib><title>Modification of human pericardium by chemical crosslinking</title><title>Physiological research</title><addtitle>Physiol Res</addtitle><description>Autologous and allogenic human pericardia used as biomaterials for cardiovascular surgery are traditionally crosslinked with glutaraldehyde. In this work, we have evaluated the resistivity to collagenase digestion and the cytotoxicity of human pericardium crosslinked with various concentrations of glutaraldehyde in comparison with pericardium crosslinked by genipin, nordihydroguaiaretic acid, tannic acid, and in comparison with unmodified pericardium. Crosslinking retained the wavy-like morphology of native pericardium visualized by second harmonic generation microscopy. The collagenase digestion products were analyzed using SDS-PAGE, capillary electrophoresis, and a hydroxyproline assay. Glutaraldehyde and genipin crosslinking protected the native pericardium efficiently against digestion with collagenase III. Only low protection was provided by the other crosslinking agents. The cytotoxicity of crosslinked pericardium was evaluated using xCELLigence by monitoring the viability of porcine valve interstitial cells cultured in eluates from crosslinked pericardium. The highest cell index, reflecting both the number and the shape of the monitored cells was observed in eluates from genipin. Crosslinking pericardium grafts with genipin therefore seems to be a promising alternative procedure to the traditional crosslinking with glutaraldehyde, because it provides similarly high protection against degradation with collagenase, without cytotoxic effects.</description><subject>Acids</subject><subject>Autografts</subject><subject>Biocompatible Materials</subject><subject>Biomaterials</subject><subject>Biomedical materials</subject><subject>Calcification</subject><subject>Capillary electrophoresis</subject><subject>Collagen</subject><subject>Collagenase</subject><subject>Collagenase 3</subject><subject>Cross-Linking Reagents</subject><subject>Cytotoxicity</subject><subject>Gel electrophoresis</subject><subject>Genipin</subject><subject>Glutaral</subject><subject>Glutaraldehyde</subject><subject>Humans</subject><subject>Hydroxyproline</subject><subject>Interstitial cells</subject><subject>Iridoids</subject><subject>Masoprocol</subject><subject>Nordihydroguaiaretic acid</subject><subject>Pathogens</subject><subject>Pericardium</subject><subject>Pericardium - chemistry</subject><subject>Sodium lauryl sulfate</subject><subject>Surgery</subject><subject>Tannic acid</subject><subject>Tannins</subject><subject>Transplants - chemistry</subject><issn>0862-8408</issn><issn>1802-9973</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNpVUMtOwzAQtBCIlsIPcECROKf4kdhZDkio4iUVcendchyncUniYCdI_XuiFgqcVrs7Mzs7CF0SPGcsTeCmq7bButqbMAeWjLMjNCUZpjGAYMdoijNO4yzB2QSdhbDBmAos2CmaMJKllGKYottXV9jSatVb10aujKqhUW3UGT_OfGGHJsq3ka5MM_Z1pL0Lobbtu23X5-ikVHUwF991hlaPD6vFc7x8e3pZ3C9jzUD0sQCFQbNCg6CMQZ4Lg4nAXOUCK8qAkzQlDKjBYIwwWhugeUkIcEWpydkM3e1luyFvTKFN23tVy87bRvmtdMrK_5vWVnLtPmWW8hR4Mgpcfwt49zGY0MuNG3w7WpaUiQyShFMyougetXvRm_JwgWC5i1v-xi33cY-kq7_eDpSffNkX2RB_kQ</recordid><startdate>20200201</startdate><enddate>20200201</enddate><creator>Filová, E</creator><creator>Staňková, L</creator><creator>Eckhardt, A</creator><creator>Svobodová, J</creator><creator>Musílková, J</creator><creator>Pala, J</creator><creator>Hadraba, D</creator><creator>Brynda, E</creator><creator>Koňařík, M</creator><creator>Pirk, J</creator><creator>Bačáková, L</creator><general>Institute of Physiology</general><general>Institute of Physiology of the Czech Academy of Sciences</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>4T-</scope><scope>4U-</scope><scope>7RV</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>BYOGL</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB0</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>M7P</scope><scope>MBDVC</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>5PM</scope></search><sort><creationdate>20200201</creationdate><title>Modification of human pericardium by chemical crosslinking</title><author>Filová, E ; Staňková, L ; Eckhardt, A ; Svobodová, J ; Musílková, J ; Pala, J ; Hadraba, D ; Brynda, E ; Koňařík, M ; Pirk, J ; Bačáková, L</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c397t-79a09c3dc972339bb7e01706ab70a23961551392e09ee7ecce92bf1196a22eb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Acids</topic><topic>Autografts</topic><topic>Biocompatible Materials</topic><topic>Biomaterials</topic><topic>Biomedical materials</topic><topic>Calcification</topic><topic>Capillary electrophoresis</topic><topic>Collagen</topic><topic>Collagenase</topic><topic>Collagenase 3</topic><topic>Cross-Linking Reagents</topic><topic>Cytotoxicity</topic><topic>Gel electrophoresis</topic><topic>Genipin</topic><topic>Glutaral</topic><topic>Glutaraldehyde</topic><topic>Humans</topic><topic>Hydroxyproline</topic><topic>Interstitial cells</topic><topic>Iridoids</topic><topic>Masoprocol</topic><topic>Nordihydroguaiaretic acid</topic><topic>Pathogens</topic><topic>Pericardium</topic><topic>Pericardium - 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In this work, we have evaluated the resistivity to collagenase digestion and the cytotoxicity of human pericardium crosslinked with various concentrations of glutaraldehyde in comparison with pericardium crosslinked by genipin, nordihydroguaiaretic acid, tannic acid, and in comparison with unmodified pericardium. Crosslinking retained the wavy-like morphology of native pericardium visualized by second harmonic generation microscopy. The collagenase digestion products were analyzed using SDS-PAGE, capillary electrophoresis, and a hydroxyproline assay. Glutaraldehyde and genipin crosslinking protected the native pericardium efficiently against digestion with collagenase III. Only low protection was provided by the other crosslinking agents. The cytotoxicity of crosslinked pericardium was evaluated using xCELLigence by monitoring the viability of porcine valve interstitial cells cultured in eluates from crosslinked pericardium. The highest cell index, reflecting both the number and the shape of the monitored cells was observed in eluates from genipin. Crosslinking pericardium grafts with genipin therefore seems to be a promising alternative procedure to the traditional crosslinking with glutaraldehyde, because it provides similarly high protection against degradation with collagenase, without cytotoxic effects.</abstract><cop>Czech Republic</cop><pub>Institute of Physiology</pub><pmid>31852209</pmid><doi>10.33549/physiolres.934335</doi><tpages>11</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Acids Autografts Biocompatible Materials Biomaterials Biomedical materials Calcification Capillary electrophoresis Collagen Collagenase Collagenase 3 Cross-Linking Reagents Cytotoxicity Gel electrophoresis Genipin Glutaral Glutaraldehyde Humans Hydroxyproline Interstitial cells Iridoids Masoprocol Nordihydroguaiaretic acid Pathogens Pericardium Pericardium - chemistry Sodium lauryl sulfate Surgery Tannic acid Tannins Transplants - chemistry |
| title | Modification of human pericardium by chemical crosslinking |
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