Streamlined CRISPR genome engineering in wild-type bacteria using SIBR-Cas

Abstract CRISPR-Cas is a powerful tool for genome editing in bacteria. However, its efficacy is dependent on host factors (such as DNA repair pathways) and/or exogenous expression of recombinases. In this study, we mitigated these constraints by developing a simple and widely applicable genome engin...

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Veröffentlicht in:	Nucleic acids research 2021-11, Vol.49 (19), p.11392-11404
Hauptverfasser:	Patinios, Constantinos, Creutzburg, Sjoerd C A, Arifah, Adini Q, Adiego-Pérez, Belén, Gyimah, Evans A, Ingham, Colin J, Kengen, Servé W M, van der Oost, John, Staals, Raymond H J
Format:	Artikel
Sprache:	eng
Schlagworte:	Base Pairing Base Sequence CRISPR-Cas Systems Escherichia coli - genetics Escherichia coli - metabolism Flavobacterium - genetics Flavobacterium - metabolism Gene Editing - methods Gene Knockout Techniques - methods Genome, Bacterial Homologous Recombination Introns Nucleic Acid Conformation Pseudomonas putida - genetics Pseudomonas putida - metabolism Riboswitch RNA Splicing RNA, Bacterial - genetics RNA, Bacterial - metabolism Synthetic Biology and Bioengineering
Online-Zugang:	Volltext
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