Alginate-Based Bioinks for 3D Bioprinting and Fabrication of Anatomically Accurate Bone Grafts
To realize the promise of three-dimensional (3D) bioprinting, it is imperative to develop bioinks that possess the necessary biological and rheological characteristics for printing cell-laden tissue grafts. Alginate is widely used as a bioink because its rheological properties can be modified throug...
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| Veröffentlicht in: | Tissue engineering. Part A 2021-09, Vol.27 (17-18), p.1168-1181 |
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| creator | Gonzalez-Fernandez, Tomas Tenorio, Alejandro J Campbell, Kevin T Silva, Eduardo A Leach, J Kent |
| description | To realize the promise of three-dimensional (3D) bioprinting, it is imperative to develop bioinks that possess the necessary biological and rheological characteristics for printing cell-laden tissue grafts. Alginate is widely used as a bioink because its rheological properties can be modified through precrosslinking or the addition of thickening agents to increase printing resolution. However, modification of alginate's physiochemical characteristics using common crosslinking agents can affect its cytocompatibility. Therefore, we evaluated the printability, physicochemical properties, and osteogenic potential of four common alginate bioinks: alginate-CaCl
2
(alg-CaCl
2
), alginate-CaSO
4
(alg-CaSO
4
), alginate-gelatin (alg-gel), and alginate-nanocellulose (alg-ncel) for the 3D bioprinting of anatomically accurate osteogenic grafts. While all bioinks possessed similar viscosity, printing fidelity was lower in the precrosslinked bioinks. When used to print geometrically defined constructs, alg-CaSO
4
and alg-ncel exhibited higher mechanical properties and lower mesh size than those printed with alg-CaCl
2
or alg-gel. The physical properties of these constructs affected the biological performance of encapsulated bone marrow-derived mesenchymal stromal cells (MSCs). Cell-laden constructs printed using alg-CaSO
4
and alg-ncel exhibited greater cell apoptosis and contained fewer living cells 7 days postprinting. In addition, effective cell–matrix interactions were only observed in alg-CaCl
2
printed constructs. When cultured in osteogenic media, MSCs in alg-CaCl
2
constructs exhibited increased osteogenic differentiation compared to the other three bioinks. This bioink was then used to 3D print anatomically accurate cell-laden scaphoid bones that were capable of partial mineralization after 14 days of
in vitro
culture. These results highlight the importance of bioink properties to modulate cell behavior and the biofabrication of clinically relevant bone tissues. |
| doi_str_mv | 10.1089/ten.tea.2020.0305 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_8558068</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2571942087</sourcerecordid><originalsourceid>FETCH-LOGICAL-c523t-5a59e32181c40eb4521e4657e591b2e07a5fea2830667645c353d63f3eb060d33</originalsourceid><addsrcrecordid>eNqNUU1r3DAUFKEhX-0PyKUIeunFG31Ykn0J7KZJWgj00kJPFbL8vFHilRJJLuTfR2aTpempByG9p5lhhkHolJIFJU17lsEvMpgFI4wsCCdiDx3RlquKc_Hr3e5d00N0nNIdIZJIpQ7QIeeMNqxlR-j3clw7bzJUK5OgxysXnL9PeAgR8y_z-BCdz86vsfE9vjJddNZkFzwOA14WZtiUxTg-4aW1UyxKeBU84Otohpzeo_3BjAk-vNwn6OfV5Y-Lr9XN9-tvF8ubygrGcyWMaGH2RG1NoKsFo1BLoUC0tGNAlBEDGNZwIqWStbBc8F7ygUNXMvWcn6Dzre7D1G2gt-BzNKMu3jcmPulgnH77492tXoc_uhGiIbIpAp9fBGJ4nCBlvXHJwjgaD2FKmtWSUyIErQv00z_QuzBFX-JpJhRta0YaVVB0i7IxpBRh2JmhRM_t6dJeOUbP7em5vcL5-HeKHeO1rgJQW8C8Nt6PDjqI-T-knwFFR6lI</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2571942087</pqid></control><display><type>article</type><title>Alginate-Based Bioinks for 3D Bioprinting and Fabrication of Anatomically Accurate Bone Grafts</title><source>Alma/SFX Local Collection</source><creator>Gonzalez-Fernandez, Tomas ; Tenorio, Alejandro J ; Campbell, Kevin T ; Silva, Eduardo A ; Leach, J Kent</creator><creatorcontrib>Gonzalez-Fernandez, Tomas ; Tenorio, Alejandro J ; Campbell, Kevin T ; Silva, Eduardo A ; Leach, J Kent</creatorcontrib><description>To realize the promise of three-dimensional (3D) bioprinting, it is imperative to develop bioinks that possess the necessary biological and rheological characteristics for printing cell-laden tissue grafts. Alginate is widely used as a bioink because its rheological properties can be modified through precrosslinking or the addition of thickening agents to increase printing resolution. However, modification of alginate's physiochemical characteristics using common crosslinking agents can affect its cytocompatibility. Therefore, we evaluated the printability, physicochemical properties, and osteogenic potential of four common alginate bioinks: alginate-CaCl
2
(alg-CaCl
2
), alginate-CaSO
4
(alg-CaSO
4
), alginate-gelatin (alg-gel), and alginate-nanocellulose (alg-ncel) for the 3D bioprinting of anatomically accurate osteogenic grafts. While all bioinks possessed similar viscosity, printing fidelity was lower in the precrosslinked bioinks. When used to print geometrically defined constructs, alg-CaSO
4
and alg-ncel exhibited higher mechanical properties and lower mesh size than those printed with alg-CaCl
2
or alg-gel. The physical properties of these constructs affected the biological performance of encapsulated bone marrow-derived mesenchymal stromal cells (MSCs). Cell-laden constructs printed using alg-CaSO
4
and alg-ncel exhibited greater cell apoptosis and contained fewer living cells 7 days postprinting. In addition, effective cell–matrix interactions were only observed in alg-CaCl
2
printed constructs. When cultured in osteogenic media, MSCs in alg-CaCl
2
constructs exhibited increased osteogenic differentiation compared to the other three bioinks. This bioink was then used to 3D print anatomically accurate cell-laden scaphoid bones that were capable of partial mineralization after 14 days of
in vitro
culture. These results highlight the importance of bioink properties to modulate cell behavior and the biofabrication of clinically relevant bone tissues.</description><identifier>ISSN: 1937-3341</identifier><identifier>EISSN: 1937-335X</identifier><identifier>DOI: 10.1089/ten.tea.2020.0305</identifier><identifier>PMID: 33218292</identifier><language>eng</language><publisher>United States: Mary Ann Liebert, Inc., publishers</publisher><subject>3-D printers ; Alginic acid ; Apoptosis ; Biocompatibility ; Bone marrow ; Calcium chloride ; Cell culture ; Cell survival ; Gelatin ; Mechanical properties ; Mesenchyme ; Mineralization ; Original ; Original Articles ; Osteogenesis ; Physical properties ; Physicochemical properties ; Printing ; Rheology ; Skin & tissue grafts ; Stromal cells</subject><ispartof>Tissue engineering. Part A, 2021-09, Vol.27 (17-18), p.1168-1181</ispartof><rights>2021, Mary Ann Liebert, Inc., publishers</rights><rights>Copyright Mary Ann Liebert, Inc. Sep 2021</rights><rights>Copyright 2021, Mary Ann Liebert, Inc., publishers 2021 Mary Ann Liebert, Inc., publishers</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c523t-5a59e32181c40eb4521e4657e591b2e07a5fea2830667645c353d63f3eb060d33</citedby><cites>FETCH-LOGICAL-c523t-5a59e32181c40eb4521e4657e591b2e07a5fea2830667645c353d63f3eb060d33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,780,784,885,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33218292$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gonzalez-Fernandez, Tomas</creatorcontrib><creatorcontrib>Tenorio, Alejandro J</creatorcontrib><creatorcontrib>Campbell, Kevin T</creatorcontrib><creatorcontrib>Silva, Eduardo A</creatorcontrib><creatorcontrib>Leach, J Kent</creatorcontrib><title>Alginate-Based Bioinks for 3D Bioprinting and Fabrication of Anatomically Accurate Bone Grafts</title><title>Tissue engineering. Part A</title><addtitle>Tissue Eng Part A</addtitle><description>To realize the promise of three-dimensional (3D) bioprinting, it is imperative to develop bioinks that possess the necessary biological and rheological characteristics for printing cell-laden tissue grafts. Alginate is widely used as a bioink because its rheological properties can be modified through precrosslinking or the addition of thickening agents to increase printing resolution. However, modification of alginate's physiochemical characteristics using common crosslinking agents can affect its cytocompatibility. Therefore, we evaluated the printability, physicochemical properties, and osteogenic potential of four common alginate bioinks: alginate-CaCl
2
(alg-CaCl
2
), alginate-CaSO
4
(alg-CaSO
4
), alginate-gelatin (alg-gel), and alginate-nanocellulose (alg-ncel) for the 3D bioprinting of anatomically accurate osteogenic grafts. While all bioinks possessed similar viscosity, printing fidelity was lower in the precrosslinked bioinks. When used to print geometrically defined constructs, alg-CaSO
4
and alg-ncel exhibited higher mechanical properties and lower mesh size than those printed with alg-CaCl
2
or alg-gel. The physical properties of these constructs affected the biological performance of encapsulated bone marrow-derived mesenchymal stromal cells (MSCs). Cell-laden constructs printed using alg-CaSO
4
and alg-ncel exhibited greater cell apoptosis and contained fewer living cells 7 days postprinting. In addition, effective cell–matrix interactions were only observed in alg-CaCl
2
printed constructs. When cultured in osteogenic media, MSCs in alg-CaCl
2
constructs exhibited increased osteogenic differentiation compared to the other three bioinks. This bioink was then used to 3D print anatomically accurate cell-laden scaphoid bones that were capable of partial mineralization after 14 days of
in vitro
culture. These results highlight the importance of bioink properties to modulate cell behavior and the biofabrication of clinically relevant bone tissues.</description><subject>3-D printers</subject><subject>Alginic acid</subject><subject>Apoptosis</subject><subject>Biocompatibility</subject><subject>Bone marrow</subject><subject>Calcium chloride</subject><subject>Cell culture</subject><subject>Cell survival</subject><subject>Gelatin</subject><subject>Mechanical properties</subject><subject>Mesenchyme</subject><subject>Mineralization</subject><subject>Original</subject><subject>Original Articles</subject><subject>Osteogenesis</subject><subject>Physical properties</subject><subject>Physicochemical properties</subject><subject>Printing</subject><subject>Rheology</subject><subject>Skin & tissue grafts</subject><subject>Stromal cells</subject><issn>1937-3341</issn><issn>1937-335X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><recordid>eNqNUU1r3DAUFKEhX-0PyKUIeunFG31Ykn0J7KZJWgj00kJPFbL8vFHilRJJLuTfR2aTpempByG9p5lhhkHolJIFJU17lsEvMpgFI4wsCCdiDx3RlquKc_Hr3e5d00N0nNIdIZJIpQ7QIeeMNqxlR-j3clw7bzJUK5OgxysXnL9PeAgR8y_z-BCdz86vsfE9vjJddNZkFzwOA14WZtiUxTg-4aW1UyxKeBU84Otohpzeo_3BjAk-vNwn6OfV5Y-Lr9XN9-tvF8ubygrGcyWMaGH2RG1NoKsFo1BLoUC0tGNAlBEDGNZwIqWStbBc8F7ygUNXMvWcn6Dzre7D1G2gt-BzNKMu3jcmPulgnH77492tXoc_uhGiIbIpAp9fBGJ4nCBlvXHJwjgaD2FKmtWSUyIErQv00z_QuzBFX-JpJhRta0YaVVB0i7IxpBRh2JmhRM_t6dJeOUbP7em5vcL5-HeKHeO1rgJQW8C8Nt6PDjqI-T-knwFFR6lI</recordid><startdate>20210901</startdate><enddate>20210901</enddate><creator>Gonzalez-Fernandez, Tomas</creator><creator>Tenorio, Alejandro J</creator><creator>Campbell, Kevin T</creator><creator>Silva, Eduardo A</creator><creator>Leach, J Kent</creator><general>Mary Ann Liebert, Inc., publishers</general><general>Mary Ann Liebert, Inc</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7T5</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>H94</scope><scope>K9.</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20210901</creationdate><title>Alginate-Based Bioinks for 3D Bioprinting and Fabrication of Anatomically Accurate Bone Grafts</title><author>Gonzalez-Fernandez, Tomas ; Tenorio, Alejandro J ; Campbell, Kevin T ; Silva, Eduardo A ; Leach, J Kent</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c523t-5a59e32181c40eb4521e4657e591b2e07a5fea2830667645c353d63f3eb060d33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>3-D printers</topic><topic>Alginic acid</topic><topic>Apoptosis</topic><topic>Biocompatibility</topic><topic>Bone marrow</topic><topic>Calcium chloride</topic><topic>Cell culture</topic><topic>Cell survival</topic><topic>Gelatin</topic><topic>Mechanical properties</topic><topic>Mesenchyme</topic><topic>Mineralization</topic><topic>Original</topic><topic>Original Articles</topic><topic>Osteogenesis</topic><topic>Physical properties</topic><topic>Physicochemical properties</topic><topic>Printing</topic><topic>Rheology</topic><topic>Skin & tissue grafts</topic><topic>Stromal cells</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gonzalez-Fernandez, Tomas</creatorcontrib><creatorcontrib>Tenorio, Alejandro J</creatorcontrib><creatorcontrib>Campbell, Kevin T</creatorcontrib><creatorcontrib>Silva, Eduardo A</creatorcontrib><creatorcontrib>Leach, J Kent</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Immunology Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Tissue engineering. Part A</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gonzalez-Fernandez, Tomas</au><au>Tenorio, Alejandro J</au><au>Campbell, Kevin T</au><au>Silva, Eduardo A</au><au>Leach, J Kent</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Alginate-Based Bioinks for 3D Bioprinting and Fabrication of Anatomically Accurate Bone Grafts</atitle><jtitle>Tissue engineering. Part A</jtitle><addtitle>Tissue Eng Part A</addtitle><date>2021-09-01</date><risdate>2021</risdate><volume>27</volume><issue>17-18</issue><spage>1168</spage><epage>1181</epage><pages>1168-1181</pages><issn>1937-3341</issn><eissn>1937-335X</eissn><abstract>To realize the promise of three-dimensional (3D) bioprinting, it is imperative to develop bioinks that possess the necessary biological and rheological characteristics for printing cell-laden tissue grafts. Alginate is widely used as a bioink because its rheological properties can be modified through precrosslinking or the addition of thickening agents to increase printing resolution. However, modification of alginate's physiochemical characteristics using common crosslinking agents can affect its cytocompatibility. Therefore, we evaluated the printability, physicochemical properties, and osteogenic potential of four common alginate bioinks: alginate-CaCl
2
(alg-CaCl
2
), alginate-CaSO
4
(alg-CaSO
4
), alginate-gelatin (alg-gel), and alginate-nanocellulose (alg-ncel) for the 3D bioprinting of anatomically accurate osteogenic grafts. While all bioinks possessed similar viscosity, printing fidelity was lower in the precrosslinked bioinks. When used to print geometrically defined constructs, alg-CaSO
4
and alg-ncel exhibited higher mechanical properties and lower mesh size than those printed with alg-CaCl
2
or alg-gel. The physical properties of these constructs affected the biological performance of encapsulated bone marrow-derived mesenchymal stromal cells (MSCs). Cell-laden constructs printed using alg-CaSO
4
and alg-ncel exhibited greater cell apoptosis and contained fewer living cells 7 days postprinting. In addition, effective cell–matrix interactions were only observed in alg-CaCl
2
printed constructs. When cultured in osteogenic media, MSCs in alg-CaCl
2
constructs exhibited increased osteogenic differentiation compared to the other three bioinks. This bioink was then used to 3D print anatomically accurate cell-laden scaphoid bones that were capable of partial mineralization after 14 days of
in vitro
culture. These results highlight the importance of bioink properties to modulate cell behavior and the biofabrication of clinically relevant bone tissues.</abstract><cop>United States</cop><pub>Mary Ann Liebert, Inc., publishers</pub><pmid>33218292</pmid><doi>10.1089/ten.tea.2020.0305</doi><tpages>14</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1937-3341 |
| ispartof | Tissue engineering. Part A, 2021-09, Vol.27 (17-18), p.1168-1181 |
| issn | 1937-3341 1937-335X |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_8558068 |
| source | Alma/SFX Local Collection |
| subjects | 3-D printers Alginic acid Apoptosis Biocompatibility Bone marrow Calcium chloride Cell culture Cell survival Gelatin Mechanical properties Mesenchyme Mineralization Original Original Articles Osteogenesis Physical properties Physicochemical properties Printing Rheology Skin & tissue grafts Stromal cells |
| title | Alginate-Based Bioinks for 3D Bioprinting and Fabrication of Anatomically Accurate Bone Grafts |
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