ZNF410 Uniquely Activates the NuRD Component CHD4 to Silence Fetal Hemoglobin Expression

Metazoan transcription factors typically regulate large numbers of genes. Here we identify via a CRISPR-Cas9 genetic screen ZNF410, a pentadactyl DNA-binding protein that in human erythroid cells directly activates only a single gene, the NuRD component CHD4. Specificity is conveyed by two highly ev...

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Veröffentlicht in:	Molecular cell 2021-01, Vol.81 (2), p.239-254.e8
Hauptverfasser:	Lan, Xianjiang, Ren, Ren, Feng, Ruopeng, Ly, Lana C., Lan, Yemin, Zhang, Zhe, Aboreden, Nicholas, Qin, Kunhua, Horton, John R., Grevet, Jeremy D., Mayuranathan, Thiyagaraj, Abdulmalik, Osheiza, Keller, Cheryl A., Giardine, Belinda, Hardison, Ross C., Crossley, Merlin, Weiss, Mitchell J., Cheng, Xiaodong, Shi, Junwei, Blobel, Gerd A.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Binding Sites CHD4 Chlorocebus aethiops COS Cells CRISPR screen CRISPR-Cas Systems DNA - genetics DNA - metabolism erythroid biology Erythroid Precursor Cells - cytology Erythroid Precursor Cells - metabolism Erythroid Precursor Cells - transplantation Fetal Blood - cytology Fetal Blood - metabolism fetal hemoglobin Fetal Hemoglobin - genetics Fetal Hemoglobin - metabolism Fetus Gene Editing HEK293 Cells hemoglobin switching Heterografts Humans Mi-2 Nucleosome Remodeling and Deacetylase Complex - chemistry Mi-2 Nucleosome Remodeling and Deacetylase Complex - genetics Mi-2 Nucleosome Remodeling and Deacetylase Complex - metabolism Mice Models, Molecular Mouse Embryonic Stem Cells - cytology NuRD Protein Binding Protein Conformation, alpha-Helical Protein Conformation, beta-Strand Protein Interaction Domains and Motifs sickle cell disease transcription transcription factor Transcription Factors - chemistry Transcription Factors - genetics Transcription Factors - metabolism Transcriptional Activation ZNF410
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