In vitro assessment of the impact of nickel on the viability and steroidogenesis in the human adrenocortical carcinoma (NCI-H295R) cell line

Nickel is a ubiquitous environmental pollutant, which has various effects on reproductive endocrinology. In this study, human adrenocortical carcinoma (NCI-H295R) cell line was used as an in vitro biological model to study the effect of nickel chloride (NiCl2) on the viability and steroidogenesis. T...

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Veröffentlicht in:Physiological research 2020-11, Vol.69 (5), p.871-883
Hauptverfasser: Lukac, N, Forgacs, Z, Duranova, H, Jambor, T, Zemanova, J, Massanyi, P, Tombarkiewicz, B, Roychoudhury, S, Knazicka, Z
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container_end_page 883
container_issue 5
container_start_page 871
container_title Physiological research
container_volume 69
creator Lukac, N
Forgacs, Z
Duranova, H
Jambor, T
Zemanova, J
Massanyi, P
Tombarkiewicz, B
Roychoudhury, S
Knazicka, Z
description Nickel is a ubiquitous environmental pollutant, which has various effects on reproductive endocrinology. In this study, human adrenocortical carcinoma (NCI-H295R) cell line was used as an in vitro biological model to study the effect of nickel chloride (NiCl2) on the viability and steroidogenesis. The cells were exposed to different concentrations (3.90; 7.80; 15.60; 31.20; 62.50; 125; 250 and 500 microM) of NiCl2 and compared with control group (culture medium without NiCl2). The cell viability was measured by the metabolic activity assay. Production of sexual steroid hormones was quantified by enzyme linked immunosorbent assay. Following 48 h culture of the cells in the presence of NiCl2 a dose-dependent depletion of progesterone release was observed even at the lower concentrations. In fact, lower levels of progesterone were detected in groups with higher doses (>/=125 microM) of NiCl2 (P
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In this study, human adrenocortical carcinoma (NCI-H295R) cell line was used as an in vitro biological model to study the effect of nickel chloride (NiCl2) on the viability and steroidogenesis. The cells were exposed to different concentrations (3.90; 7.80; 15.60; 31.20; 62.50; 125; 250 and 500 microM) of NiCl2 and compared with control group (culture medium without NiCl2). The cell viability was measured by the metabolic activity assay. Production of sexual steroid hormones was quantified by enzyme linked immunosorbent assay. Following 48 h culture of the cells in the presence of NiCl2 a dose-dependent depletion of progesterone release was observed even at the lower concentrations. In fact, lower levels of progesterone were detected in groups with higher doses (&gt;/=125 microM) of NiCl2 (P&lt;0.01), which also elicited cytotoxic action. A more prominent decrease in testosterone production (P&lt;0.01) was also noted in comparison to that of progesterone. On the other hand, the release of 17beta-estradiol was substantially increased at low concentrations (3.90 to 62.50 microM) of NiCl2. The cell viability remained relatively unaltered up to 125 microM (P&gt;0.05) and slightly decreased from 250 microM of NiCl2 (P&lt;0.05). Our results indicate endocrine disruptive effect of NiCl2 on the release of progesterone and testosterone in the NCI-H295R cell line. Although no detrimental effect of NiCl2 (&lt;/=62.50 microM) could be found on 17beta-estradiol production, its toxicity may reflect at other points of the steroidogenic pathway.</description><identifier>ISSN: 0862-8408</identifier><identifier>EISSN: 1802-9973</identifier><identifier>DOI: 10.33549/PHYSIOLRES.934452</identifier><identifier>PMID: 32901497</identifier><language>eng</language><publisher>Czech Republic: Institute of Physiology</publisher><subject>17β-Estradiol ; Adrenal Cortex Neoplasms - metabolism ; Adrenal Cortex Neoplasms - pathology ; Adrenocortical Carcinoma - metabolism ; Adrenocortical Carcinoma - pathology ; Cancer ; Cell culture ; Cell Line, Tumor ; Cell Survival ; Cell viability ; Cytotoxicity ; Endocrine Disruptors - pharmacology ; Endocrinology ; Enzyme-linked immunosorbent assay ; Estradiol - metabolism ; Hormones ; Humans ; In Vitro Techniques ; Laboratories ; Neuroendocrine tumors ; Nickel - pharmacology ; Nickel chloride ; Physiology ; Pollutants ; Progesterone ; Progesterone - metabolism ; Steroid hormones ; Steroidogenesis ; Steroids ; Testosterone ; Testosterone - metabolism</subject><ispartof>Physiological research, 2020-11, Vol.69 (5), p.871-883</ispartof><rights>Copyright Institute of Physiology 2020</rights><rights>2020 Institute of Physiology of the Czech Academy of Sciences, Prague, Czech Republic 2020</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c397t-6ba6226d777cabf900600ed547ec9854a43fb2d256e06b1a7a584980b667ae773</citedby><cites>FETCH-LOGICAL-c397t-6ba6226d777cabf900600ed547ec9854a43fb2d256e06b1a7a584980b667ae773</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8549914/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8549914/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,724,777,781,861,882,27905,27906,53772,53774</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32901497$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lukac, N</creatorcontrib><creatorcontrib>Forgacs, Z</creatorcontrib><creatorcontrib>Duranova, H</creatorcontrib><creatorcontrib>Jambor, T</creatorcontrib><creatorcontrib>Zemanova, J</creatorcontrib><creatorcontrib>Massanyi, P</creatorcontrib><creatorcontrib>Tombarkiewicz, B</creatorcontrib><creatorcontrib>Roychoudhury, S</creatorcontrib><creatorcontrib>Knazicka, Z</creatorcontrib><title>In vitro assessment of the impact of nickel on the viability and steroidogenesis in the human adrenocortical carcinoma (NCI-H295R) cell line</title><title>Physiological research</title><addtitle>Physiol Res</addtitle><description>Nickel is a ubiquitous environmental pollutant, which has various effects on reproductive endocrinology. In this study, human adrenocortical carcinoma (NCI-H295R) cell line was used as an in vitro biological model to study the effect of nickel chloride (NiCl2) on the viability and steroidogenesis. The cells were exposed to different concentrations (3.90; 7.80; 15.60; 31.20; 62.50; 125; 250 and 500 microM) of NiCl2 and compared with control group (culture medium without NiCl2). The cell viability was measured by the metabolic activity assay. Production of sexual steroid hormones was quantified by enzyme linked immunosorbent assay. Following 48 h culture of the cells in the presence of NiCl2 a dose-dependent depletion of progesterone release was observed even at the lower concentrations. In fact, lower levels of progesterone were detected in groups with higher doses (&gt;/=125 microM) of NiCl2 (P&lt;0.01), which also elicited cytotoxic action. A more prominent decrease in testosterone production (P&lt;0.01) was also noted in comparison to that of progesterone. 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Although no detrimental effect of NiCl2 (&lt;/=62.50 microM) could be found on 17beta-estradiol production, its toxicity may reflect at other points of the steroidogenic pathway.</description><subject>17β-Estradiol</subject><subject>Adrenal Cortex Neoplasms - metabolism</subject><subject>Adrenal Cortex Neoplasms - pathology</subject><subject>Adrenocortical Carcinoma - metabolism</subject><subject>Adrenocortical Carcinoma - pathology</subject><subject>Cancer</subject><subject>Cell culture</subject><subject>Cell Line, Tumor</subject><subject>Cell Survival</subject><subject>Cell viability</subject><subject>Cytotoxicity</subject><subject>Endocrine Disruptors - pharmacology</subject><subject>Endocrinology</subject><subject>Enzyme-linked immunosorbent assay</subject><subject>Estradiol - metabolism</subject><subject>Hormones</subject><subject>Humans</subject><subject>In Vitro Techniques</subject><subject>Laboratories</subject><subject>Neuroendocrine tumors</subject><subject>Nickel - pharmacology</subject><subject>Nickel chloride</subject><subject>Physiology</subject><subject>Pollutants</subject><subject>Progesterone</subject><subject>Progesterone - metabolism</subject><subject>Steroid hormones</subject><subject>Steroidogenesis</subject><subject>Steroids</subject><subject>Testosterone</subject><subject>Testosterone - metabolism</subject><issn>0862-8408</issn><issn>1802-9973</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNpVUU1vEzEQtRAVDYU_wAFZ4gKHLV7ba68vSCgqJFJEUT8OnKxZ72zjsmun9iZS_gM_mlXSVnAazcx7b97oEfKuZOdCVNJ8_rn4db28XF1dXJ8bIWXFX5BZWTNeGKPFSzJjteJFLVl9Sl7nfM8Y10yLV-RUcMNKafSM_FkGuvNjihRyxpwHDCONHR3XSP2wAXfogne_sacxHOY7D43v_binEFqaR0zRt_EOA2afqT-C1tsBAoU2YYguptE76KmD5HyIA9CPP-bLYsFNdfWJOux72vuAb8hJB33Gt4_1jNx-u7iZL4rV5ffl_OuqcMLosVANKM5Vq7V20HSGMcUYtpXU6ExdSZCia3jLK4VMNSVoqGppatYopQG1Fmfky1F3s20GbN30c4LebpIfIO1tBG__3wS_tndxZydxY0o5CXx4FEjxYYt5tPdxm8Lk2XLFdWkUk2JC8SPKpZhzwu75QsnsIUG7We-zj33CbI8JTqT3_3p7pjxFJv4Cs-SbKw</recordid><startdate>20201116</startdate><enddate>20201116</enddate><creator>Lukac, N</creator><creator>Forgacs, Z</creator><creator>Duranova, H</creator><creator>Jambor, T</creator><creator>Zemanova, J</creator><creator>Massanyi, P</creator><creator>Tombarkiewicz, B</creator><creator>Roychoudhury, S</creator><creator>Knazicka, Z</creator><general>Institute of Physiology</general><general>Institute of Physiology of the Czech Academy of Sciences</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>4T-</scope><scope>4U-</scope><scope>7RV</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>BYOGL</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB0</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>M7P</scope><scope>MBDVC</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>5PM</scope></search><sort><creationdate>20201116</creationdate><title>In vitro assessment of the impact of nickel on the viability and steroidogenesis in the human adrenocortical carcinoma (NCI-H295R) cell line</title><author>Lukac, N ; 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Medical Complete (Alumni)</collection><collection>Nursing &amp; Allied Health Database (Alumni Edition)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health &amp; Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Research Library</collection><collection>Biological Science Database</collection><collection>Research Library (Corporate)</collection><collection>Nursing &amp; Allied Health Premium</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Physiological research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lukac, N</au><au>Forgacs, Z</au><au>Duranova, H</au><au>Jambor, T</au><au>Zemanova, J</au><au>Massanyi, P</au><au>Tombarkiewicz, B</au><au>Roychoudhury, S</au><au>Knazicka, Z</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>In vitro assessment of the impact of nickel on the viability and steroidogenesis in the human adrenocortical carcinoma (NCI-H295R) cell line</atitle><jtitle>Physiological research</jtitle><addtitle>Physiol Res</addtitle><date>2020-11-16</date><risdate>2020</risdate><volume>69</volume><issue>5</issue><spage>871</spage><epage>883</epage><pages>871-883</pages><issn>0862-8408</issn><eissn>1802-9973</eissn><abstract>Nickel is a ubiquitous environmental pollutant, which has various effects on reproductive endocrinology. In this study, human adrenocortical carcinoma (NCI-H295R) cell line was used as an in vitro biological model to study the effect of nickel chloride (NiCl2) on the viability and steroidogenesis. The cells were exposed to different concentrations (3.90; 7.80; 15.60; 31.20; 62.50; 125; 250 and 500 microM) of NiCl2 and compared with control group (culture medium without NiCl2). The cell viability was measured by the metabolic activity assay. Production of sexual steroid hormones was quantified by enzyme linked immunosorbent assay. Following 48 h culture of the cells in the presence of NiCl2 a dose-dependent depletion of progesterone release was observed even at the lower concentrations. In fact, lower levels of progesterone were detected in groups with higher doses (&gt;/=125 microM) of NiCl2 (P&lt;0.01), which also elicited cytotoxic action. A more prominent decrease in testosterone production (P&lt;0.01) was also noted in comparison to that of progesterone. On the other hand, the release of 17beta-estradiol was substantially increased at low concentrations (3.90 to 62.50 microM) of NiCl2. The cell viability remained relatively unaltered up to 125 microM (P&gt;0.05) and slightly decreased from 250 microM of NiCl2 (P&lt;0.05). Our results indicate endocrine disruptive effect of NiCl2 on the release of progesterone and testosterone in the NCI-H295R cell line. Although no detrimental effect of NiCl2 (&lt;/=62.50 microM) could be found on 17beta-estradiol production, its toxicity may reflect at other points of the steroidogenic pathway.</abstract><cop>Czech Republic</cop><pub>Institute of Physiology</pub><pmid>32901497</pmid><doi>10.33549/PHYSIOLRES.934452</doi><tpages>13</tpages><oa>free_for_read</oa></addata></record>
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subjects 17β-Estradiol
Adrenal Cortex Neoplasms - metabolism
Adrenal Cortex Neoplasms - pathology
Adrenocortical Carcinoma - metabolism
Adrenocortical Carcinoma - pathology
Cancer
Cell culture
Cell Line, Tumor
Cell Survival
Cell viability
Cytotoxicity
Endocrine Disruptors - pharmacology
Endocrinology
Enzyme-linked immunosorbent assay
Estradiol - metabolism
Hormones
Humans
In Vitro Techniques
Laboratories
Neuroendocrine tumors
Nickel - pharmacology
Nickel chloride
Physiology
Pollutants
Progesterone
Progesterone - metabolism
Steroid hormones
Steroidogenesis
Steroids
Testosterone
Testosterone - metabolism
title In vitro assessment of the impact of nickel on the viability and steroidogenesis in the human adrenocortical carcinoma (NCI-H295R) cell line
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