Protein Arginine Methyltransferase 5 Promotes the Migration of AML Cells by Regulating the Expression of Leukocyte Immunoglobulin-Like Receptor B4
Acute myeloid leukemia (AML) is the most common type of acute leukemia in adults with poor prognosis. Especially for AML-M5 type, due to the strong cell migration ability, the possibility of extramedullary invasion is large and widespread, which leads to poor therapeutic effect. Previous studies hav...
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| description | Acute myeloid leukemia (AML) is the most common type of acute leukemia in adults with poor prognosis. Especially for AML-M5 type, due to the strong cell migration ability, the possibility of extramedullary invasion is large and widespread, which leads to poor therapeutic effect. Previous studies have found that protein arginine methyltransferase 5 (PRMT5) could promote the proliferation and differentiation of leukemic cells in AML, but its regulation on the invasive ability of AML cells remains unclear. This study was designed to explore the role of PRMT5 in regulating the invasion of AML cells and to investigate the mechanisms. Patient samples were collected for detection of PRMT5 expression level. AML cells were used for exploring the function of PRMT5. The results of clinical samples showed that the expression of PRMT5 was significantly increased in newly diagnosed and recurrent AML patients, and the expression of leukocyte immunoglobulin-like receptor B4 (LILRB4) was positively correlated with the level of PRMT5. In the cell experiment in vitro, we found that when PRMT5 was knocked down, the invasion, migration, and adhesion capacities of MV-4-11 cells and THP-1 cells were decreased, and the mRNA and protein levels of LILRB4 were also decreased. Moreover, we screened related signaling pathways and found that PRMT5 affected the expression of downstream LILRB4 by activating mTOR pathway, which in turn enhanced the invasive ability of AML cells. Taken together, PRMT5 plays an important role in the invasion of AML, which acts via regulating the expression of LILRB4. PRMT5 could act as a potential therapeutic candidate for AML. |
| doi_str_mv | 10.1155/2021/7329072 |
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Especially for AML-M5 type, due to the strong cell migration ability, the possibility of extramedullary invasion is large and widespread, which leads to poor therapeutic effect. Previous studies have found that protein arginine methyltransferase 5 (PRMT5) could promote the proliferation and differentiation of leukemic cells in AML, but its regulation on the invasive ability of AML cells remains unclear. This study was designed to explore the role of PRMT5 in regulating the invasion of AML cells and to investigate the mechanisms. Patient samples were collected for detection of PRMT5 expression level. AML cells were used for exploring the function of PRMT5. The results of clinical samples showed that the expression of PRMT5 was significantly increased in newly diagnosed and recurrent AML patients, and the expression of leukocyte immunoglobulin-like receptor B4 (LILRB4) was positively correlated with the level of PRMT5. In the cell experiment in vitro, we found that when PRMT5 was knocked down, the invasion, migration, and adhesion capacities of MV-4-11 cells and THP-1 cells were decreased, and the mRNA and protein levels of LILRB4 were also decreased. Moreover, we screened related signaling pathways and found that PRMT5 affected the expression of downstream LILRB4 by activating mTOR pathway, which in turn enhanced the invasive ability of AML cells. Taken together, PRMT5 plays an important role in the invasion of AML, which acts via regulating the expression of LILRB4. PRMT5 could act as a potential therapeutic candidate for AML.</description><identifier>ISSN: 2314-6133</identifier><identifier>EISSN: 2314-6141</identifier><identifier>DOI: 10.1155/2021/7329072</identifier><identifier>PMID: 34712735</identifier><language>eng</language><publisher>United States: Hindawi</publisher><subject>Acute myeloid leukemia ; Adult ; Aged ; Analysis ; Antibodies ; Bone marrow ; Cancer cells ; Cell adhesion ; Cell Adhesion - drug effects ; Cell Adhesion - genetics ; Cell differentiation ; Cell Line, Tumor ; Cell migration ; Cell Movement - drug effects ; Cell Movement - genetics ; Cell proliferation ; Down-Regulation - drug effects ; Down-Regulation - genetics ; Enzymes ; Extracellular Signal-Regulated MAP Kinases - metabolism ; Female ; Gene expression ; Gene Expression Regulation, Leukemic - drug effects ; Genetic aspects ; Hospitals ; Humans ; Identification and classification ; Immunoglobulins ; Invasiveness ; Isoquinolines - pharmacology ; Laboratories ; Leukemia ; Leukemia, Myeloid, Acute - genetics ; Leukemia, Myeloid, Acute - pathology ; Leukocytes ; Male ; Medical prognosis ; Membrane Glycoproteins - genetics ; Membrane Glycoproteins - metabolism ; Membrane proteins ; Middle Aged ; mRNA ; Myeloid leukemia ; Neoplasm Invasiveness ; Patients ; Phosphatase ; Properties ; Protein arginine methyltransferase ; Protein-Arginine N-Methyltransferases - metabolism ; Proteins ; Proto-Oncogene Proteins c-akt - metabolism ; Pyrimidines - pharmacology ; Receptors ; Receptors, Immunologic - genetics ; Receptors, Immunologic - metabolism ; Signal transduction ; Signal Transduction - drug effects ; Stem cells ; TOR protein ; TOR Serine-Threonine Kinases - metabolism ; Transplants & implants ; Young Adult</subject><ispartof>BioMed research international, 2021, Vol.2021 (1), p.7329072</ispartof><rights>Copyright © 2021 Lu Zhao et al.</rights><rights>COPYRIGHT 2021 John Wiley & Sons, Inc.</rights><rights>Copyright © 2021 Lu Zhao et al. This is an open access article distributed under the Creative Commons Attribution License (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. https://creativecommons.org/licenses/by/4.0</rights><rights>Copyright © 2021 Lu Zhao et al. 2021</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c504t-bf36e4bc7ac4b8fa1281a6464ef6971e19fb65deb51507b108f31d22ba44fd653</citedby><cites>FETCH-LOGICAL-c504t-bf36e4bc7ac4b8fa1281a6464ef6971e19fb65deb51507b108f31d22ba44fd653</cites><orcidid>0000-0001-5043-9626 ; 0000-0003-1550-989X ; 0000-0001-6298-9406 ; 0000-0002-8548-2832 ; 0000-0002-5568-2884 ; 0000-0002-9220-7898 ; 0000-0002-5107-382X ; 0000-0003-1535-8809</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8548104/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8548104/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,4010,27902,27903,27904,53769,53771</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34712735$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Yang, Jun</contributor><contributor>Jun Yang</contributor><creatorcontrib>Zhao, Lu</creatorcontrib><creatorcontrib>Cheng, Bingqing</creatorcontrib><creatorcontrib>Xiong, Jie</creatorcontrib><creatorcontrib>Ma, Dan</creatorcontrib><creatorcontrib>Liu, Xin</creatorcontrib><creatorcontrib>Wang, Li</creatorcontrib><creatorcontrib>Zhang, Xi</creatorcontrib><creatorcontrib>Wang, Jishi</creatorcontrib><title>Protein Arginine Methyltransferase 5 Promotes the Migration of AML Cells by Regulating the Expression of Leukocyte Immunoglobulin-Like Receptor B4</title><title>BioMed research international</title><addtitle>Biomed Res Int</addtitle><description>Acute myeloid leukemia (AML) is the most common type of acute leukemia in adults with poor prognosis. Especially for AML-M5 type, due to the strong cell migration ability, the possibility of extramedullary invasion is large and widespread, which leads to poor therapeutic effect. Previous studies have found that protein arginine methyltransferase 5 (PRMT5) could promote the proliferation and differentiation of leukemic cells in AML, but its regulation on the invasive ability of AML cells remains unclear. This study was designed to explore the role of PRMT5 in regulating the invasion of AML cells and to investigate the mechanisms. Patient samples were collected for detection of PRMT5 expression level. AML cells were used for exploring the function of PRMT5. The results of clinical samples showed that the expression of PRMT5 was significantly increased in newly diagnosed and recurrent AML patients, and the expression of leukocyte immunoglobulin-like receptor B4 (LILRB4) was positively correlated with the level of PRMT5. In the cell experiment in vitro, we found that when PRMT5 was knocked down, the invasion, migration, and adhesion capacities of MV-4-11 cells and THP-1 cells were decreased, and the mRNA and protein levels of LILRB4 were also decreased. Moreover, we screened related signaling pathways and found that PRMT5 affected the expression of downstream LILRB4 by activating mTOR pathway, which in turn enhanced the invasive ability of AML cells. Taken together, PRMT5 plays an important role in the invasion of AML, which acts via regulating the expression of LILRB4. PRMT5 could act as a potential therapeutic candidate for AML.</description><subject>Acute myeloid leukemia</subject><subject>Adult</subject><subject>Aged</subject><subject>Analysis</subject><subject>Antibodies</subject><subject>Bone marrow</subject><subject>Cancer cells</subject><subject>Cell adhesion</subject><subject>Cell Adhesion - drug effects</subject><subject>Cell Adhesion - genetics</subject><subject>Cell differentiation</subject><subject>Cell Line, Tumor</subject><subject>Cell migration</subject><subject>Cell Movement - drug effects</subject><subject>Cell Movement - genetics</subject><subject>Cell proliferation</subject><subject>Down-Regulation - drug effects</subject><subject>Down-Regulation - genetics</subject><subject>Enzymes</subject><subject>Extracellular Signal-Regulated MAP Kinases - metabolism</subject><subject>Female</subject><subject>Gene expression</subject><subject>Gene Expression Regulation, Leukemic - drug effects</subject><subject>Genetic aspects</subject><subject>Hospitals</subject><subject>Humans</subject><subject>Identification and classification</subject><subject>Immunoglobulins</subject><subject>Invasiveness</subject><subject>Isoquinolines - pharmacology</subject><subject>Laboratories</subject><subject>Leukemia</subject><subject>Leukemia, Myeloid, Acute - genetics</subject><subject>Leukemia, Myeloid, Acute - pathology</subject><subject>Leukocytes</subject><subject>Male</subject><subject>Medical prognosis</subject><subject>Membrane Glycoproteins - genetics</subject><subject>Membrane Glycoproteins - metabolism</subject><subject>Membrane proteins</subject><subject>Middle Aged</subject><subject>mRNA</subject><subject>Myeloid leukemia</subject><subject>Neoplasm Invasiveness</subject><subject>Patients</subject><subject>Phosphatase</subject><subject>Properties</subject><subject>Protein arginine methyltransferase</subject><subject>Protein-Arginine N-Methyltransferases - metabolism</subject><subject>Proteins</subject><subject>Proto-Oncogene Proteins c-akt - metabolism</subject><subject>Pyrimidines - pharmacology</subject><subject>Receptors</subject><subject>Receptors, Immunologic - genetics</subject><subject>Receptors, Immunologic - metabolism</subject><subject>Signal transduction</subject><subject>Signal Transduction - drug effects</subject><subject>Stem cells</subject><subject>TOR protein</subject><subject>TOR Serine-Threonine Kinases - metabolism</subject><subject>Transplants & implants</subject><subject>Young 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Arginine Methyltransferase 5 Promotes the Migration of AML Cells by Regulating the Expression of Leukocyte Immunoglobulin-Like Receptor B4</title><author>Zhao, Lu ; Cheng, Bingqing ; Xiong, Jie ; Ma, Dan ; Liu, Xin ; Wang, Li ; Zhang, Xi ; Wang, Jishi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c504t-bf36e4bc7ac4b8fa1281a6464ef6971e19fb65deb51507b108f31d22ba44fd653</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Acute myeloid leukemia</topic><topic>Adult</topic><topic>Aged</topic><topic>Analysis</topic><topic>Antibodies</topic><topic>Bone marrow</topic><topic>Cancer cells</topic><topic>Cell adhesion</topic><topic>Cell Adhesion - drug effects</topic><topic>Cell Adhesion - genetics</topic><topic>Cell differentiation</topic><topic>Cell Line, Tumor</topic><topic>Cell migration</topic><topic>Cell Movement - drug effects</topic><topic>Cell Movement - genetics</topic><topic>Cell proliferation</topic><topic>Down-Regulation - drug effects</topic><topic>Down-Regulation - genetics</topic><topic>Enzymes</topic><topic>Extracellular Signal-Regulated MAP Kinases - metabolism</topic><topic>Female</topic><topic>Gene expression</topic><topic>Gene Expression Regulation, Leukemic - drug effects</topic><topic>Genetic aspects</topic><topic>Hospitals</topic><topic>Humans</topic><topic>Identification and classification</topic><topic>Immunoglobulins</topic><topic>Invasiveness</topic><topic>Isoquinolines - pharmacology</topic><topic>Laboratories</topic><topic>Leukemia</topic><topic>Leukemia, Myeloid, Acute - genetics</topic><topic>Leukemia, Myeloid, Acute - pathology</topic><topic>Leukocytes</topic><topic>Male</topic><topic>Medical prognosis</topic><topic>Membrane Glycoproteins - genetics</topic><topic>Membrane Glycoproteins - metabolism</topic><topic>Membrane proteins</topic><topic>Middle Aged</topic><topic>mRNA</topic><topic>Myeloid 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of AML Cells by Regulating the Expression of Leukocyte Immunoglobulin-Like Receptor B4</atitle><jtitle>BioMed research international</jtitle><addtitle>Biomed Res Int</addtitle><date>2021</date><risdate>2021</risdate><volume>2021</volume><issue>1</issue><spage>7329072</spage><pages>7329072-</pages><issn>2314-6133</issn><eissn>2314-6141</eissn><abstract>Acute myeloid leukemia (AML) is the most common type of acute leukemia in adults with poor prognosis. Especially for AML-M5 type, due to the strong cell migration ability, the possibility of extramedullary invasion is large and widespread, which leads to poor therapeutic effect. Previous studies have found that protein arginine methyltransferase 5 (PRMT5) could promote the proliferation and differentiation of leukemic cells in AML, but its regulation on the invasive ability of AML cells remains unclear. This study was designed to explore the role of PRMT5 in regulating the invasion of AML cells and to investigate the mechanisms. Patient samples were collected for detection of PRMT5 expression level. AML cells were used for exploring the function of PRMT5. The results of clinical samples showed that the expression of PRMT5 was significantly increased in newly diagnosed and recurrent AML patients, and the expression of leukocyte immunoglobulin-like receptor B4 (LILRB4) was positively correlated with the level of PRMT5. In the cell experiment in vitro, we found that when PRMT5 was knocked down, the invasion, migration, and adhesion capacities of MV-4-11 cells and THP-1 cells were decreased, and the mRNA and protein levels of LILRB4 were also decreased. Moreover, we screened related signaling pathways and found that PRMT5 affected the expression of downstream LILRB4 by activating mTOR pathway, which in turn enhanced the invasive ability of AML cells. Taken together, PRMT5 plays an important role in the invasion of AML, which acts via regulating the expression of LILRB4. PRMT5 could act as a potential therapeutic candidate for AML.</abstract><cop>United States</cop><pub>Hindawi</pub><pmid>34712735</pmid><doi>10.1155/2021/7329072</doi><orcidid>https://orcid.org/0000-0001-5043-9626</orcidid><orcidid>https://orcid.org/0000-0003-1550-989X</orcidid><orcidid>https://orcid.org/0000-0001-6298-9406</orcidid><orcidid>https://orcid.org/0000-0002-8548-2832</orcidid><orcidid>https://orcid.org/0000-0002-5568-2884</orcidid><orcidid>https://orcid.org/0000-0002-9220-7898</orcidid><orcidid>https://orcid.org/0000-0002-5107-382X</orcidid><orcidid>https://orcid.org/0000-0003-1535-8809</orcidid><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Wiley Online Library Open Access; PubMed Central; Alma/SFX Local Collection; PubMed Central Open Access |
| subjects | Acute myeloid leukemia Adult Aged Analysis Antibodies Bone marrow Cancer cells Cell adhesion Cell Adhesion - drug effects Cell Adhesion - genetics Cell differentiation Cell Line, Tumor Cell migration Cell Movement - drug effects Cell Movement - genetics Cell proliferation Down-Regulation - drug effects Down-Regulation - genetics Enzymes Extracellular Signal-Regulated MAP Kinases - metabolism Female Gene expression Gene Expression Regulation, Leukemic - drug effects Genetic aspects Hospitals Humans Identification and classification Immunoglobulins Invasiveness Isoquinolines - pharmacology Laboratories Leukemia Leukemia, Myeloid, Acute - genetics Leukemia, Myeloid, Acute - pathology Leukocytes Male Medical prognosis Membrane Glycoproteins - genetics Membrane Glycoproteins - metabolism Membrane proteins Middle Aged mRNA Myeloid leukemia Neoplasm Invasiveness Patients Phosphatase Properties Protein arginine methyltransferase Protein-Arginine N-Methyltransferases - metabolism Proteins Proto-Oncogene Proteins c-akt - metabolism Pyrimidines - pharmacology Receptors Receptors, Immunologic - genetics Receptors, Immunologic - metabolism Signal transduction Signal Transduction - drug effects Stem cells TOR protein TOR Serine-Threonine Kinases - metabolism Transplants & implants Young Adult |
| title | Protein Arginine Methyltransferase 5 Promotes the Migration of AML Cells by Regulating the Expression of Leukocyte Immunoglobulin-Like Receptor B4 |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-27T20%3A50%3A00IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Protein%20Arginine%20Methyltransferase%205%20Promotes%20the%20Migration%20of%20AML%20Cells%20by%20Regulating%20the%20Expression%20of%20Leukocyte%20Immunoglobulin-Like%20Receptor%20B4&rft.jtitle=BioMed%20research%20international&rft.au=Zhao,%20Lu&rft.date=2021&rft.volume=2021&rft.issue=1&rft.spage=7329072&rft.pages=7329072-&rft.issn=2314-6133&rft.eissn=2314-6141&rft_id=info:doi/10.1155/2021/7329072&rft_dat=%3Cgale_pubme%3EA696844222%3C/gale_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2589570699&rft_id=info:pmid/34712735&rft_galeid=A696844222&rfr_iscdi=true |