Femtosecond diode-based time lens laser for multiphoton microscopy
We demonstrate a near-infrared, femtosecond, diode laser-based source with kW peak power for two-photon microscopy. At a wavelength of 976 nm, the system produces sub-ps pulses operating at a repetition rate of 10 MHz with kilowatt class peak powers suitable for deep tissue two-photon microscopy. Th...
Gespeichert in:
| Veröffentlicht in: | Biomedical optics express 2021-10, Vol.12 (10), p.6269-6276 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 6276 |
|---|---|
| container_issue | 10 |
| container_start_page | 6269 |
| container_title | Biomedical optics express |
| container_volume | 12 |
| creator | Lange Simmons, Y. Underwood, Kenneth J. Supekar, Omkar D. Heffernan, Brendan M. Welton, Tarah A. Gibson, Emily A. Gopinath, Juliet T. |
| description | We demonstrate a near-infrared, femtosecond, diode laser-based source with kW peak power for two-photon microscopy. At a wavelength of 976 nm, the system produces sub-ps pulses operating at a repetition rate of 10 MHz with kilowatt class peak powers suitable for deep tissue two-photon microscopy. The system, integrated with a laser-scanning microscope, images to a depth of 900 µm in a fixed sample of PLP-eGFP labeled mouse brain tissue. This represents a significant development that will lead to more efficient, compact, and accessible laser sources for biomedical imaging. |
| doi_str_mv | 10.1364/BOE.430549 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_8548023</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2595116163</sourcerecordid><originalsourceid>FETCH-LOGICAL-c314t-960d071ba286db575f25557a991acc803128d6da826cab77993d1fffd488fd7b3</originalsourceid><addsrcrecordid>eNpVkE1Lw0AQhhdRbKm9-AtyFCF1N_uZi2BLq0KhFz0vm_2wK0k27iZC_70pLaJzmRnm5X2ZB4BbBBcIM_Kw3K0XBENKygswLRBlOYeCXv6ZJ2Ce0iccixAOsbgGE0w4oRzTKVhubNOHZHVoTWZ8MDavVLIm631js9q2KavHPWYuxKwZ6t53-9CHNmu8jiHp0B1uwJVTdbLzc5-B9836bfWSb3fPr6unba4xIn1eMmggR5UqBDMV5dQVlFKuyhIprQXEqBCGGSUKplXFeVlig5xzhgjhDK_wDDyefLuhaqzRtu2jqmUXfaPiQQbl5f9L6_fyI3xLQYmABR4N7s4GMXwNNvWy8UnbulatDUOSBS0pQgyxo_T-JD0-maJ1vzEIyiN3OXKXJ-74B96sdJM</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2595116163</pqid></control><display><type>article</type><title>Femtosecond diode-based time lens laser for multiphoton microscopy</title><source>DOAJ Directory of Open Access Journals</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><creator>Lange Simmons, Y. ; Underwood, Kenneth J. ; Supekar, Omkar D. ; Heffernan, Brendan M. ; Welton, Tarah A. ; Gibson, Emily A. ; Gopinath, Juliet T.</creator><creatorcontrib>Lange Simmons, Y. ; Underwood, Kenneth J. ; Supekar, Omkar D. ; Heffernan, Brendan M. ; Welton, Tarah A. ; Gibson, Emily A. ; Gopinath, Juliet T.</creatorcontrib><description>We demonstrate a near-infrared, femtosecond, diode laser-based source with kW peak power for two-photon microscopy. At a wavelength of 976 nm, the system produces sub-ps pulses operating at a repetition rate of 10 MHz with kilowatt class peak powers suitable for deep tissue two-photon microscopy. The system, integrated with a laser-scanning microscope, images to a depth of 900 µm in a fixed sample of PLP-eGFP labeled mouse brain tissue. This represents a significant development that will lead to more efficient, compact, and accessible laser sources for biomedical imaging.</description><identifier>ISSN: 2156-7085</identifier><identifier>EISSN: 2156-7085</identifier><identifier>DOI: 10.1364/BOE.430549</identifier><identifier>PMID: 34745735</identifier><language>eng</language><publisher>Optical Society of America</publisher><ispartof>Biomedical optics express, 2021-10, Vol.12 (10), p.6269-6276</ispartof><rights>2021 Optical Society of America under the terms of the 2021 Optical Society of America</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c314t-960d071ba286db575f25557a991acc803128d6da826cab77993d1fffd488fd7b3</cites><orcidid>0000-0003-2387-5371 ; 0000-0002-2392-1887 ; 0000-0003-1777-8508</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8548023/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8548023/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,864,885,27924,27925,53791,53793</link.rule.ids></links><search><creatorcontrib>Lange Simmons, Y.</creatorcontrib><creatorcontrib>Underwood, Kenneth J.</creatorcontrib><creatorcontrib>Supekar, Omkar D.</creatorcontrib><creatorcontrib>Heffernan, Brendan M.</creatorcontrib><creatorcontrib>Welton, Tarah A.</creatorcontrib><creatorcontrib>Gibson, Emily A.</creatorcontrib><creatorcontrib>Gopinath, Juliet T.</creatorcontrib><title>Femtosecond diode-based time lens laser for multiphoton microscopy</title><title>Biomedical optics express</title><description>We demonstrate a near-infrared, femtosecond, diode laser-based source with kW peak power for two-photon microscopy. At a wavelength of 976 nm, the system produces sub-ps pulses operating at a repetition rate of 10 MHz with kilowatt class peak powers suitable for deep tissue two-photon microscopy. The system, integrated with a laser-scanning microscope, images to a depth of 900 µm in a fixed sample of PLP-eGFP labeled mouse brain tissue. This represents a significant development that will lead to more efficient, compact, and accessible laser sources for biomedical imaging.</description><issn>2156-7085</issn><issn>2156-7085</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><recordid>eNpVkE1Lw0AQhhdRbKm9-AtyFCF1N_uZi2BLq0KhFz0vm_2wK0k27iZC_70pLaJzmRnm5X2ZB4BbBBcIM_Kw3K0XBENKygswLRBlOYeCXv6ZJ2Ce0iccixAOsbgGE0w4oRzTKVhubNOHZHVoTWZ8MDavVLIm631js9q2KavHPWYuxKwZ6t53-9CHNmu8jiHp0B1uwJVTdbLzc5-B9836bfWSb3fPr6unba4xIn1eMmggR5UqBDMV5dQVlFKuyhIprQXEqBCGGSUKplXFeVlig5xzhgjhDK_wDDyefLuhaqzRtu2jqmUXfaPiQQbl5f9L6_fyI3xLQYmABR4N7s4GMXwNNvWy8UnbulatDUOSBS0pQgyxo_T-JD0-maJ1vzEIyiN3OXKXJ-74B96sdJM</recordid><startdate>20211001</startdate><enddate>20211001</enddate><creator>Lange Simmons, Y.</creator><creator>Underwood, Kenneth J.</creator><creator>Supekar, Omkar D.</creator><creator>Heffernan, Brendan M.</creator><creator>Welton, Tarah A.</creator><creator>Gibson, Emily A.</creator><creator>Gopinath, Juliet T.</creator><general>Optical Society of America</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0003-2387-5371</orcidid><orcidid>https://orcid.org/0000-0002-2392-1887</orcidid><orcidid>https://orcid.org/0000-0003-1777-8508</orcidid></search><sort><creationdate>20211001</creationdate><title>Femtosecond diode-based time lens laser for multiphoton microscopy</title><author>Lange Simmons, Y. ; Underwood, Kenneth J. ; Supekar, Omkar D. ; Heffernan, Brendan M. ; Welton, Tarah A. ; Gibson, Emily A. ; Gopinath, Juliet T.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c314t-960d071ba286db575f25557a991acc803128d6da826cab77993d1fffd488fd7b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lange Simmons, Y.</creatorcontrib><creatorcontrib>Underwood, Kenneth J.</creatorcontrib><creatorcontrib>Supekar, Omkar D.</creatorcontrib><creatorcontrib>Heffernan, Brendan M.</creatorcontrib><creatorcontrib>Welton, Tarah A.</creatorcontrib><creatorcontrib>Gibson, Emily A.</creatorcontrib><creatorcontrib>Gopinath, Juliet T.</creatorcontrib><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Biomedical optics express</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lange Simmons, Y.</au><au>Underwood, Kenneth J.</au><au>Supekar, Omkar D.</au><au>Heffernan, Brendan M.</au><au>Welton, Tarah A.</au><au>Gibson, Emily A.</au><au>Gopinath, Juliet T.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Femtosecond diode-based time lens laser for multiphoton microscopy</atitle><jtitle>Biomedical optics express</jtitle><date>2021-10-01</date><risdate>2021</risdate><volume>12</volume><issue>10</issue><spage>6269</spage><epage>6276</epage><pages>6269-6276</pages><issn>2156-7085</issn><eissn>2156-7085</eissn><abstract>We demonstrate a near-infrared, femtosecond, diode laser-based source with kW peak power for two-photon microscopy. At a wavelength of 976 nm, the system produces sub-ps pulses operating at a repetition rate of 10 MHz with kilowatt class peak powers suitable for deep tissue two-photon microscopy. The system, integrated with a laser-scanning microscope, images to a depth of 900 µm in a fixed sample of PLP-eGFP labeled mouse brain tissue. This represents a significant development that will lead to more efficient, compact, and accessible laser sources for biomedical imaging.</abstract><pub>Optical Society of America</pub><pmid>34745735</pmid><doi>10.1364/BOE.430549</doi><tpages>8</tpages><orcidid>https://orcid.org/0000-0003-2387-5371</orcidid><orcidid>https://orcid.org/0000-0002-2392-1887</orcidid><orcidid>https://orcid.org/0000-0003-1777-8508</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 2156-7085 |
| ispartof | Biomedical optics express, 2021-10, Vol.12 (10), p.6269-6276 |
| issn | 2156-7085 2156-7085 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_8548023 |
| source | DOAJ Directory of Open Access Journals; EZB-FREE-00999 freely available EZB journals; PubMed Central |
| title | Femtosecond diode-based time lens laser for multiphoton microscopy |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-29T15%3A12%3A12IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Femtosecond%20diode-based%20time%20lens%20laser%20for%20multiphoton%20microscopy&rft.jtitle=Biomedical%20optics%20express&rft.au=Lange%20Simmons,%20Y.&rft.date=2021-10-01&rft.volume=12&rft.issue=10&rft.spage=6269&rft.epage=6276&rft.pages=6269-6276&rft.issn=2156-7085&rft.eissn=2156-7085&rft_id=info:doi/10.1364/BOE.430549&rft_dat=%3Cproquest_pubme%3E2595116163%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2595116163&rft_id=info:pmid/34745735&rfr_iscdi=true |