Electrochemical biosensor for detecting pathogenic bacteria based on a hybridization chain reaction and CRISPR-Cas12a

In this study, Lba Cas12a (Cpf1) as one of the CRISPR systems from Lachnospiraceae bacterium was coupled with a hybridization chain reaction (HCR) to develop an electrochemical biosensor for detecting the pathogenic bacterium, Salmonella typhimurium . Autonomous cross-opening of functional DNA hairp...

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Veröffentlicht in:	Analytical and bioanalytical chemistry 2022-01, Vol.414 (2), p.1073-1080
Hauptverfasser:	Liu, Xiu, Bu, Shengjun, Feng, Jingqi, Wei, Hongguo, Wang, Ze, Li, Xue, Zhou, Hongyu, He, Xiuxia, Wan, Jiayu
Format:	Artikel
Sprache:	eng
Schlagworte:	Analysis Analytical Chemistry Aptamers Bacteria Biochemistry Biosensing Techniques - methods Biosensors Characterization and Evaluation of Materials Chemistry Chemistry and Materials Science CRISPR CRISPR-Cas Systems Deoxyribonucleic acid Detection limits DNA Electrochemical apparatus Electrochemical Techniques - methods Electrochemistry Electron transfer Electrophoresis, Polyacrylamide Gel Food Science Genetic aspects Hybridization Identification and classification Laboratory Medicine Methods Monitoring/Environmental Analysis Polymers Research Paper Salmonella Salmonella typhimurium Salmonella typhimurium - isolation & purification Salmonella typhimurium - pathogenicity Single-stranded DNA Tags
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