Comparison of susceptibility testing methods with mecA gene analysis for determining oxacillin (Methicillin) resistance in clinical isolates of Staphylococcus aureus and coagulase-negative Staphylococcus spp
Ninety-nine clinical staphylococcal isolates (58 coagulase-negative Staphylococcus spp. [CoNS] and 41 Staphylococcus aureus isolates) were evaluated for susceptibility to oxacillin. The following susceptibility testing methods, media, and incubation conditions were studied: agar dilution by using Mu...
Gespeichert in:
| Veröffentlicht in: | Journal of clinical microbiology 1999-09, Vol.37 (9), p.2952-2961 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 2961 |
|---|---|
| container_issue | 9 |
| container_start_page | 2952 |
| container_title | Journal of clinical microbiology |
| container_volume | 37 |
| creator | KOHNER, P UHL, J KOLBERT, C PERSING, D III, F. C |
| description | Ninety-nine clinical staphylococcal isolates (58 coagulase-negative Staphylococcus spp. [CoNS] and 41 Staphylococcus aureus isolates) were evaluated for susceptibility to oxacillin. The following susceptibility testing methods, media, and incubation conditions were studied: agar dilution by using Mueller-Hinton (MH) medium (Difco) supplemented with either 0, 2, or 4% NaCl and incubation at 30 or 35 degrees C in ambient air for 24 or 48 h; disk diffusion by using commercially prepared MH medium (Difco) and MH II agar (BBL) and incubation at 35 degrees C in ambient air for 24 or 48 h; and agar screen (spot or swab inoculation) by using commercially prepared agar (Remel) or MH agar (Difco) prepared in-house, each containing 4% NaCl and 6 microg of oxacillin/ml (0.6-microg/ml oxacillin was also studied with MH agar prepared in-house for the agar swab method and CoNS isolates) and incubation at 35 degrees C in ambient air for 24 or 48 h for swab inoculation and at 30 or 35 degrees C in ambient air for 24 or 48 h for spot inoculation. The results for these methods were compared to the results for mecA gene detection by a PCR method. Given the ability to support growth and the results for susceptibility testing (the breakpoint for susceptible isolates was |
| doi_str_mv | 10.1128/jcm.37.9.2952-2961.1999 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_85421</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>69976626</sourcerecordid><originalsourceid>FETCH-LOGICAL-c525t-688d75cef979c5165cbb6b87636b667c24aa8484870d3a97bc8fa705a86263f23</originalsourceid><addsrcrecordid>eNqFks-O1SAUxhujca6jr6AsjNFFK5QWSuJmcuPfzMSFmrgjp5Tey4RCBTp6n9JXkube6OjGsDg58Ps4cM5XFE8Irgipu5fXaqoor0RVi7Yua8FIRYQQd4oNwaIrGcNf7xYbjEVbEkL5WfEgxmuMSdO07f3ijOCmEU1HNsXPrZ9mCCZ6h_yI4hKVnpPpjTXpgJKOybgdmnTa-yGi7ybtc6Iu0E47jcCBPUQT0egDGnTSYTJu5f0PUMZa49Dzqyw1x-QFCjrTCZzSKJ-pvGcUWJSrW8i11hd8SjDvD9Yrr9QSESxBr8ENSHnYLRaiLp3eQTI3-l84zvPD4t4INupHp3hefHnz-vP2XXn58e377cVlqdq6TSXruoG3So-CC9US1qq-Z33HGWU9Y1zVDUDX5MXxQEHwXnUjcNxCx2pGx5qeF6-O985LP-lBaZcCWDkHM0E4SA9G_n3izF7u_I3s2qYmWf7sJA_-25K7LCeTO28tOO2XKJkQnK2l_gcSTmvWUZpBfgRV8DEGPf5-C8Fy9Yz8sL2SlEshV8_I1TNy9UxWPr79lVu6o0ky8PQEQMzjGkMeoIl_OEEbUmP6C31r0vo</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>17326833</pqid></control><display><type>article</type><title>Comparison of susceptibility testing methods with mecA gene analysis for determining oxacillin (Methicillin) resistance in clinical isolates of Staphylococcus aureus and coagulase-negative Staphylococcus spp</title><source>American Society for Microbiology</source><source>MEDLINE</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>PubMed Central</source><creator>KOHNER, P ; UHL, J ; KOLBERT, C ; PERSING, D ; III, F. C</creator><creatorcontrib>KOHNER, P ; UHL, J ; KOLBERT, C ; PERSING, D ; III, F. C</creatorcontrib><description>Ninety-nine clinical staphylococcal isolates (58 coagulase-negative Staphylococcus spp. [CoNS] and 41 Staphylococcus aureus isolates) were evaluated for susceptibility to oxacillin. The following susceptibility testing methods, media, and incubation conditions were studied: agar dilution by using Mueller-Hinton (MH) medium (Difco) supplemented with either 0, 2, or 4% NaCl and incubation at 30 or 35 degrees C in ambient air for 24 or 48 h; disk diffusion by using commercially prepared MH medium (Difco) and MH II agar (BBL) and incubation at 35 degrees C in ambient air for 24 or 48 h; and agar screen (spot or swab inoculation) by using commercially prepared agar (Remel) or MH agar (Difco) prepared in-house, each containing 4% NaCl and 6 microg of oxacillin/ml (0.6-microg/ml oxacillin was also studied with MH agar prepared in-house for the agar swab method and CoNS isolates) and incubation at 35 degrees C in ambient air for 24 or 48 h for swab inoculation and at 30 or 35 degrees C in ambient air for 24 or 48 h for spot inoculation. The results for these methods were compared to the results for mecA gene detection by a PCR method. Given the ability to support growth and the results for susceptibility testing (the breakpoint for susceptible isolates was </=2 microg/ml), the best methods for CoNS isolates were (i) agar dilution by using MH medium supplemented with 4% NaCl and incubation at 35 degrees C for 48 h (no growth failures were noted, and sensitivity was 97.6%) and (ii) agar screen (swab inoculation) by using MH medium prepared in-house supplemented with 4% NaCl and containing 0.6 microg oxacillin/ml and incubation at 35 degrees C for 48 h (one isolate that did not carry the mecA gene did not grow, and the sensitivity was 100%). All but one (agar dilution without added NaCl and incubation at 30 degrees C for 48 h) of the methods tested revealed all oxacillin-resistant S. aureus isolates, and no growth failures occurred with any method. If the breakpoint for susceptibility was lowered to </=1 microg/ml for agar dilution methods, more CoNS isolates with oxacillin resistance related to the mecA gene were detected when 0 or 2% NaCl agar supplementation was used. Only one CoNS isolate with mecA gene-associated resistance was not detected by using agar dilution and MH medium supplemented with 4% NaCl with incubation for 48 h. When the breakpoint for susceptibility was decreased 10-fold (from 6.0 to 0.6 microg of oxacillin per ml) for the agar swab screen method, fully 100% of the CoNS isolates that carried the mecA gene were identified.</description><identifier>ISSN: 0095-1137</identifier><identifier>EISSN: 1098-660X</identifier><identifier>DOI: 10.1128/jcm.37.9.2952-2961.1999</identifier><identifier>PMID: 10449481</identifier><identifier>CODEN: JCMIDW</identifier><language>eng</language><publisher>Washington, DC: American Society for Microbiology</publisher><subject>Antibacterial agents ; Antibiotics. Antiinfectious agents. Antiparasitic agents ; Bacterial Proteins - genetics ; Bacteriology ; Biological and medical sciences ; Carrier Proteins - genetics ; Coagulase - metabolism ; Genes, Bacterial ; Hexosyltransferases ; Humans ; Medical sciences ; Microbial Sensitivity Tests - methods ; Muramoylpentapeptide Carboxypeptidase - genetics ; Oxacillin - pharmacology ; Penicillin Resistance ; Penicillin-Binding Proteins ; Peptidyl Transferases ; Pharmacology. Drug treatments ; Sodium Chloride - pharmacology ; Staphylococcus ; Staphylococcus - drug effects ; Staphylococcus aureus ; Staphylococcus aureus - drug effects</subject><ispartof>Journal of clinical microbiology, 1999-09, Vol.37 (9), p.2952-2961</ispartof><rights>1999 INIST-CNRS</rights><rights>Copyright © 1999, American Society for Microbiology 1999</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c525t-688d75cef979c5165cbb6b87636b667c24aa8484870d3a97bc8fa705a86263f23</citedby><cites>FETCH-LOGICAL-c525t-688d75cef979c5165cbb6b87636b667c24aa8484870d3a97bc8fa705a86263f23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC85421/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC85421/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,315,728,781,785,886,3189,27929,27930,53796,53798</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1934120$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10449481$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>KOHNER, P</creatorcontrib><creatorcontrib>UHL, J</creatorcontrib><creatorcontrib>KOLBERT, C</creatorcontrib><creatorcontrib>PERSING, D</creatorcontrib><creatorcontrib>III, F. C</creatorcontrib><title>Comparison of susceptibility testing methods with mecA gene analysis for determining oxacillin (Methicillin) resistance in clinical isolates of Staphylococcus aureus and coagulase-negative Staphylococcus spp</title><title>Journal of clinical microbiology</title><addtitle>J Clin Microbiol</addtitle><description>Ninety-nine clinical staphylococcal isolates (58 coagulase-negative Staphylococcus spp. [CoNS] and 41 Staphylococcus aureus isolates) were evaluated for susceptibility to oxacillin. The following susceptibility testing methods, media, and incubation conditions were studied: agar dilution by using Mueller-Hinton (MH) medium (Difco) supplemented with either 0, 2, or 4% NaCl and incubation at 30 or 35 degrees C in ambient air for 24 or 48 h; disk diffusion by using commercially prepared MH medium (Difco) and MH II agar (BBL) and incubation at 35 degrees C in ambient air for 24 or 48 h; and agar screen (spot or swab inoculation) by using commercially prepared agar (Remel) or MH agar (Difco) prepared in-house, each containing 4% NaCl and 6 microg of oxacillin/ml (0.6-microg/ml oxacillin was also studied with MH agar prepared in-house for the agar swab method and CoNS isolates) and incubation at 35 degrees C in ambient air for 24 or 48 h for swab inoculation and at 30 or 35 degrees C in ambient air for 24 or 48 h for spot inoculation. The results for these methods were compared to the results for mecA gene detection by a PCR method. Given the ability to support growth and the results for susceptibility testing (the breakpoint for susceptible isolates was </=2 microg/ml), the best methods for CoNS isolates were (i) agar dilution by using MH medium supplemented with 4% NaCl and incubation at 35 degrees C for 48 h (no growth failures were noted, and sensitivity was 97.6%) and (ii) agar screen (swab inoculation) by using MH medium prepared in-house supplemented with 4% NaCl and containing 0.6 microg oxacillin/ml and incubation at 35 degrees C for 48 h (one isolate that did not carry the mecA gene did not grow, and the sensitivity was 100%). All but one (agar dilution without added NaCl and incubation at 30 degrees C for 48 h) of the methods tested revealed all oxacillin-resistant S. aureus isolates, and no growth failures occurred with any method. If the breakpoint for susceptibility was lowered to </=1 microg/ml for agar dilution methods, more CoNS isolates with oxacillin resistance related to the mecA gene were detected when 0 or 2% NaCl agar supplementation was used. Only one CoNS isolate with mecA gene-associated resistance was not detected by using agar dilution and MH medium supplemented with 4% NaCl with incubation for 48 h. When the breakpoint for susceptibility was decreased 10-fold (from 6.0 to 0.6 microg of oxacillin per ml) for the agar swab screen method, fully 100% of the CoNS isolates that carried the mecA gene were identified.</description><subject>Antibacterial agents</subject><subject>Antibiotics. Antiinfectious agents. Antiparasitic agents</subject><subject>Bacterial Proteins - genetics</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Carrier Proteins - genetics</subject><subject>Coagulase - metabolism</subject><subject>Genes, Bacterial</subject><subject>Hexosyltransferases</subject><subject>Humans</subject><subject>Medical sciences</subject><subject>Microbial Sensitivity Tests - methods</subject><subject>Muramoylpentapeptide Carboxypeptidase - genetics</subject><subject>Oxacillin - pharmacology</subject><subject>Penicillin Resistance</subject><subject>Penicillin-Binding Proteins</subject><subject>Peptidyl Transferases</subject><subject>Pharmacology. Drug treatments</subject><subject>Sodium Chloride - pharmacology</subject><subject>Staphylococcus</subject><subject>Staphylococcus - drug effects</subject><subject>Staphylococcus aureus</subject><subject>Staphylococcus aureus - drug effects</subject><issn>0095-1137</issn><issn>1098-660X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFks-O1SAUxhujca6jr6AsjNFFK5QWSuJmcuPfzMSFmrgjp5Tey4RCBTp6n9JXkube6OjGsDg58Ps4cM5XFE8Irgipu5fXaqoor0RVi7Yua8FIRYQQd4oNwaIrGcNf7xYbjEVbEkL5WfEgxmuMSdO07f3ijOCmEU1HNsXPrZ9mCCZ6h_yI4hKVnpPpjTXpgJKOybgdmnTa-yGi7ybtc6Iu0E47jcCBPUQT0egDGnTSYTJu5f0PUMZa49Dzqyw1x-QFCjrTCZzSKJ-pvGcUWJSrW8i11hd8SjDvD9Yrr9QSESxBr8ENSHnYLRaiLp3eQTI3-l84zvPD4t4INupHp3hefHnz-vP2XXn58e377cVlqdq6TSXruoG3So-CC9US1qq-Z33HGWU9Y1zVDUDX5MXxQEHwXnUjcNxCx2pGx5qeF6-O985LP-lBaZcCWDkHM0E4SA9G_n3izF7u_I3s2qYmWf7sJA_-25K7LCeTO28tOO2XKJkQnK2l_gcSTmvWUZpBfgRV8DEGPf5-C8Fy9Yz8sL2SlEshV8_I1TNy9UxWPr79lVu6o0ky8PQEQMzjGkMeoIl_OEEbUmP6C31r0vo</recordid><startdate>19990901</startdate><enddate>19990901</enddate><creator>KOHNER, P</creator><creator>UHL, J</creator><creator>KOLBERT, C</creator><creator>PERSING, D</creator><creator>III, F. C</creator><general>American Society for Microbiology</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19990901</creationdate><title>Comparison of susceptibility testing methods with mecA gene analysis for determining oxacillin (Methicillin) resistance in clinical isolates of Staphylococcus aureus and coagulase-negative Staphylococcus spp</title><author>KOHNER, P ; UHL, J ; KOLBERT, C ; PERSING, D ; III, F. C</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c525t-688d75cef979c5165cbb6b87636b667c24aa8484870d3a97bc8fa705a86263f23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Antibacterial agents</topic><topic>Antibiotics. Antiinfectious agents. Antiparasitic agents</topic><topic>Bacterial Proteins - genetics</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>Carrier Proteins - genetics</topic><topic>Coagulase - metabolism</topic><topic>Genes, Bacterial</topic><topic>Hexosyltransferases</topic><topic>Humans</topic><topic>Medical sciences</topic><topic>Microbial Sensitivity Tests - methods</topic><topic>Muramoylpentapeptide Carboxypeptidase - genetics</topic><topic>Oxacillin - pharmacology</topic><topic>Penicillin Resistance</topic><topic>Penicillin-Binding Proteins</topic><topic>Peptidyl Transferases</topic><topic>Pharmacology. Drug treatments</topic><topic>Sodium Chloride - pharmacology</topic><topic>Staphylococcus</topic><topic>Staphylococcus - drug effects</topic><topic>Staphylococcus aureus</topic><topic>Staphylococcus aureus - drug effects</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>KOHNER, P</creatorcontrib><creatorcontrib>UHL, J</creatorcontrib><creatorcontrib>KOLBERT, C</creatorcontrib><creatorcontrib>PERSING, D</creatorcontrib><creatorcontrib>III, F. C</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of clinical microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>KOHNER, P</au><au>UHL, J</au><au>KOLBERT, C</au><au>PERSING, D</au><au>III, F. C</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comparison of susceptibility testing methods with mecA gene analysis for determining oxacillin (Methicillin) resistance in clinical isolates of Staphylococcus aureus and coagulase-negative Staphylococcus spp</atitle><jtitle>Journal of clinical microbiology</jtitle><addtitle>J Clin Microbiol</addtitle><date>1999-09-01</date><risdate>1999</risdate><volume>37</volume><issue>9</issue><spage>2952</spage><epage>2961</epage><pages>2952-2961</pages><issn>0095-1137</issn><eissn>1098-660X</eissn><coden>JCMIDW</coden><abstract>Ninety-nine clinical staphylococcal isolates (58 coagulase-negative Staphylococcus spp. [CoNS] and 41 Staphylococcus aureus isolates) were evaluated for susceptibility to oxacillin. The following susceptibility testing methods, media, and incubation conditions were studied: agar dilution by using Mueller-Hinton (MH) medium (Difco) supplemented with either 0, 2, or 4% NaCl and incubation at 30 or 35 degrees C in ambient air for 24 or 48 h; disk diffusion by using commercially prepared MH medium (Difco) and MH II agar (BBL) and incubation at 35 degrees C in ambient air for 24 or 48 h; and agar screen (spot or swab inoculation) by using commercially prepared agar (Remel) or MH agar (Difco) prepared in-house, each containing 4% NaCl and 6 microg of oxacillin/ml (0.6-microg/ml oxacillin was also studied with MH agar prepared in-house for the agar swab method and CoNS isolates) and incubation at 35 degrees C in ambient air for 24 or 48 h for swab inoculation and at 30 or 35 degrees C in ambient air for 24 or 48 h for spot inoculation. The results for these methods were compared to the results for mecA gene detection by a PCR method. Given the ability to support growth and the results for susceptibility testing (the breakpoint for susceptible isolates was </=2 microg/ml), the best methods for CoNS isolates were (i) agar dilution by using MH medium supplemented with 4% NaCl and incubation at 35 degrees C for 48 h (no growth failures were noted, and sensitivity was 97.6%) and (ii) agar screen (swab inoculation) by using MH medium prepared in-house supplemented with 4% NaCl and containing 0.6 microg oxacillin/ml and incubation at 35 degrees C for 48 h (one isolate that did not carry the mecA gene did not grow, and the sensitivity was 100%). All but one (agar dilution without added NaCl and incubation at 30 degrees C for 48 h) of the methods tested revealed all oxacillin-resistant S. aureus isolates, and no growth failures occurred with any method. If the breakpoint for susceptibility was lowered to </=1 microg/ml for agar dilution methods, more CoNS isolates with oxacillin resistance related to the mecA gene were detected when 0 or 2% NaCl agar supplementation was used. Only one CoNS isolate with mecA gene-associated resistance was not detected by using agar dilution and MH medium supplemented with 4% NaCl with incubation for 48 h. When the breakpoint for susceptibility was decreased 10-fold (from 6.0 to 0.6 microg of oxacillin per ml) for the agar swab screen method, fully 100% of the CoNS isolates that carried the mecA gene were identified.</abstract><cop>Washington, DC</cop><pub>American Society for Microbiology</pub><pmid>10449481</pmid><doi>10.1128/jcm.37.9.2952-2961.1999</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0095-1137 |
| ispartof | Journal of clinical microbiology, 1999-09, Vol.37 (9), p.2952-2961 |
| issn | 0095-1137 1098-660X |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_85421 |
| source | American Society for Microbiology; MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central |
| subjects | Antibacterial agents Antibiotics. Antiinfectious agents. Antiparasitic agents Bacterial Proteins - genetics Bacteriology Biological and medical sciences Carrier Proteins - genetics Coagulase - metabolism Genes, Bacterial Hexosyltransferases Humans Medical sciences Microbial Sensitivity Tests - methods Muramoylpentapeptide Carboxypeptidase - genetics Oxacillin - pharmacology Penicillin Resistance Penicillin-Binding Proteins Peptidyl Transferases Pharmacology. Drug treatments Sodium Chloride - pharmacology Staphylococcus Staphylococcus - drug effects Staphylococcus aureus Staphylococcus aureus - drug effects |
| title | Comparison of susceptibility testing methods with mecA gene analysis for determining oxacillin (Methicillin) resistance in clinical isolates of Staphylococcus aureus and coagulase-negative Staphylococcus spp |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-13T01%3A53%3A15IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Comparison%20of%20susceptibility%20testing%20methods%20with%20mecA%20gene%20analysis%20for%20determining%20oxacillin%20(Methicillin)%20resistance%20in%20clinical%20isolates%20of%20Staphylococcus%20aureus%20and%20coagulase-negative%20Staphylococcus%20spp&rft.jtitle=Journal%20of%20clinical%20microbiology&rft.au=KOHNER,%20P&rft.date=1999-09-01&rft.volume=37&rft.issue=9&rft.spage=2952&rft.epage=2961&rft.pages=2952-2961&rft.issn=0095-1137&rft.eissn=1098-660X&rft.coden=JCMIDW&rft_id=info:doi/10.1128/jcm.37.9.2952-2961.1999&rft_dat=%3Cproquest_pubme%3E69976626%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=17326833&rft_id=info:pmid/10449481&rfr_iscdi=true |