Urethral Tissue Reconstruction Using the Acellular Dermal Matrix Patch Modified with Collagen-Binding VEGF in Beagle Urethral Injury Models
Objectives. Urethral tissue reconstruction for hypospadias is challenging for urologists. In this study, bovine acellular dermal matrix (ADM) patch loading with collagen-binding vascular endothelial growth factor (CBD-VEGF) was used to repair the urethral injury in beagles. Methods. The safety and e...
Gespeichert in:
| Veröffentlicht in: | BioMed research international 2021, Vol.2021 (1), p.5502740-5502740 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 5502740 |
|---|---|
| container_issue | 1 |
| container_start_page | 5502740 |
| container_title | BioMed research international |
| container_volume | 2021 |
| creator | Wang, Yanni Wang, Guannan Hou, Xianglin Zhao, Yannan Chen, Bing Dai, Jianwu Sun, Ning |
| description | Objectives. Urethral tissue reconstruction for hypospadias is challenging for urologists. In this study, bovine acellular dermal matrix (ADM) patch loading with collagen-binding vascular endothelial growth factor (CBD-VEGF) was used to repair the urethral injury in beagles. Methods. The safety and effectiveness of the scaffold implantation were carefully evaluated by comparing among the urethral injury control group, ADM implantation group, and ADM modified with CBD-VEGF implantation group during 6 months. Urodynamic examination, urethral angiography, and pathological examination were performed to evaluate the recovery of urethral tissue. Results. Stricture, urethral diverticulum, and increased urethral closure pressure were observed in the control group. Fistula was observed in one animal in the ADM group. By contrast, no related complications or other adverse situations were observed in animals treated with ADM patch modified with CBD-VEGF. The average urethra diameter was significantly smaller in the control animals than in scaffold implantation groups. Pathological examination revealed more distribution of proliferative blood vessels in the animals treated with ADM modified with CBD-VEGF. Conclusions. Overall, ADM patches modified with CBD-VEGF demonstrated an optimized tissue repair performance in a way to increase tissue angiogenesis and maintain urethral function without inducing severe inflammation and scar formation. |
| doi_str_mv | 10.1155/2021/5502740 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_8536433</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A683552474</galeid><sourcerecordid>A683552474</sourcerecordid><originalsourceid>FETCH-LOGICAL-c504t-32bf3c7cea1abf85208f9c9456102ef3225d4498c7cfd57ec577882e8a434d013</originalsourceid><addsrcrecordid>eNp9kktv1DAUhSMEolXpjjWyxAYJQv2Os0GaDm2p1AqEOmwtj3Mz8ShjFzuh9Dfwp3GYYXgs6o0t-bvn-F6fonhO8FtChDihmJITITCtOH5UHFJGeCkJJ4_3Z8YOiuOU1jgvRSSu5dPigHFZU8XIYfFjEWHoounRjUtpBPQZbPBpiKMdXPBokZxfoaEDNLPQ92NvInoPcZMLrs0Q3Xf0yQy2Q9ehca2DBt25oUPz0PdmBb48db6ZBL6cXZwj59EpmFUPaG966ddjvJ-qoU_Piiet6RMc7_ajYnF-djP_UF59vLicz65KKzAfSkaXLbOVBUPMslWCYtXWtuZCEkyhZZSKhvNaZaRtRAVWVJVSFJThjDeYsKPi3Vb3dlxuoLHgh_wWfRvdxsR7HYzT_9541-lV-KaVYJIzlgVe7QRi-DpCGvTGpWk8xkMYk6ZCiZrWhPKMvvwPXYcx-tzeL4rUiknxh1qZHrTzbci-dhLVM1lLxQXD8mFKMSEorybHN1vKxpBShHbfGMF6Co2eQqN3ocn4i7-HsYd_RyQDr7dAl__S3LmH5X4CJdvHvQ</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2585198365</pqid></control><display><type>article</type><title>Urethral Tissue Reconstruction Using the Acellular Dermal Matrix Patch Modified with Collagen-Binding VEGF in Beagle Urethral Injury Models</title><source>MEDLINE</source><source>PubMed Central Open Access</source><source>Wiley Online Library Open Access</source><source>PubMed Central</source><source>Alma/SFX Local Collection</source><creator>Wang, Yanni ; Wang, Guannan ; Hou, Xianglin ; Zhao, Yannan ; Chen, Bing ; Dai, Jianwu ; Sun, Ning</creator><contributor>Wang, Aijun ; Aijun Wang</contributor><creatorcontrib>Wang, Yanni ; Wang, Guannan ; Hou, Xianglin ; Zhao, Yannan ; Chen, Bing ; Dai, Jianwu ; Sun, Ning ; Wang, Aijun ; Aijun Wang</creatorcontrib><description>Objectives. Urethral tissue reconstruction for hypospadias is challenging for urologists. In this study, bovine acellular dermal matrix (ADM) patch loading with collagen-binding vascular endothelial growth factor (CBD-VEGF) was used to repair the urethral injury in beagles. Methods. The safety and effectiveness of the scaffold implantation were carefully evaluated by comparing among the urethral injury control group, ADM implantation group, and ADM modified with CBD-VEGF implantation group during 6 months. Urodynamic examination, urethral angiography, and pathological examination were performed to evaluate the recovery of urethral tissue. Results. Stricture, urethral diverticulum, and increased urethral closure pressure were observed in the control group. Fistula was observed in one animal in the ADM group. By contrast, no related complications or other adverse situations were observed in animals treated with ADM patch modified with CBD-VEGF. The average urethra diameter was significantly smaller in the control animals than in scaffold implantation groups. Pathological examination revealed more distribution of proliferative blood vessels in the animals treated with ADM modified with CBD-VEGF. Conclusions. Overall, ADM patches modified with CBD-VEGF demonstrated an optimized tissue repair performance in a way to increase tissue angiogenesis and maintain urethral function without inducing severe inflammation and scar formation.</description><identifier>ISSN: 2314-6133</identifier><identifier>EISSN: 2314-6141</identifier><identifier>DOI: 10.1155/2021/5502740</identifier><identifier>PMID: 34692831</identifier><language>eng</language><publisher>United States: Hindawi</publisher><subject>Acellular Dermis - metabolism ; Angiogenesis ; Angiography ; Animals ; Binding ; Biomedical materials ; Bladder ; Blood vessels ; Care and treatment ; Catheters ; Cattle ; Cell growth ; Collagen ; Collagen - metabolism ; Complications ; Disease Models, Animal ; Dogs ; Equipment and supplies ; Growth factors ; Health aspects ; Hypospadias ; Hypospadias - metabolism ; Hypospadias - pathology ; Hypospadias - surgery ; Implantation ; Injuries ; Male ; Medical imaging ; Methods ; Penis ; Pharmaceuticals ; Physiological aspects ; Quantitative analysis ; Reconstruction ; Reconstructive Surgical Procedures - methods ; Repair ; Scaffolds ; Skin & tissue grafts ; Software ; Surgery ; Surgery, Plastic ; Tissue engineering ; Tissue Scaffolds ; Tissues ; Urethra ; Urethra - chemistry ; Urethra - surgery ; Urethra - transplantation ; Urethral diseases ; Urinary organs ; Vascular endothelial growth factor ; Vascular Endothelial Growth Factor A - genetics ; Vascular Endothelial Growth Factor A - metabolism ; Wound healing ; Wound Healing - drug effects</subject><ispartof>BioMed research international, 2021, Vol.2021 (1), p.5502740-5502740</ispartof><rights>Copyright © 2021 Yanni Wang et al.</rights><rights>COPYRIGHT 2021 John Wiley & Sons, Inc.</rights><rights>Copyright © 2021 Yanni Wang et al. This is an open access article distributed under the Creative Commons Attribution License (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. https://creativecommons.org/licenses/by/4.0</rights><rights>Copyright © 2021 Yanni Wang et al. 2021</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c504t-32bf3c7cea1abf85208f9c9456102ef3225d4498c7cfd57ec577882e8a434d013</citedby><cites>FETCH-LOGICAL-c504t-32bf3c7cea1abf85208f9c9456102ef3225d4498c7cfd57ec577882e8a434d013</cites><orcidid>0000-0002-7963-4625 ; 0000-0002-3379-9053</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8536433/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8536433/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,4009,27902,27903,27904,53770,53772</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34692831$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Wang, Aijun</contributor><contributor>Aijun Wang</contributor><creatorcontrib>Wang, Yanni</creatorcontrib><creatorcontrib>Wang, Guannan</creatorcontrib><creatorcontrib>Hou, Xianglin</creatorcontrib><creatorcontrib>Zhao, Yannan</creatorcontrib><creatorcontrib>Chen, Bing</creatorcontrib><creatorcontrib>Dai, Jianwu</creatorcontrib><creatorcontrib>Sun, Ning</creatorcontrib><title>Urethral Tissue Reconstruction Using the Acellular Dermal Matrix Patch Modified with Collagen-Binding VEGF in Beagle Urethral Injury Models</title><title>BioMed research international</title><addtitle>Biomed Res Int</addtitle><description>Objectives. Urethral tissue reconstruction for hypospadias is challenging for urologists. In this study, bovine acellular dermal matrix (ADM) patch loading with collagen-binding vascular endothelial growth factor (CBD-VEGF) was used to repair the urethral injury in beagles. Methods. The safety and effectiveness of the scaffold implantation were carefully evaluated by comparing among the urethral injury control group, ADM implantation group, and ADM modified with CBD-VEGF implantation group during 6 months. Urodynamic examination, urethral angiography, and pathological examination were performed to evaluate the recovery of urethral tissue. Results. Stricture, urethral diverticulum, and increased urethral closure pressure were observed in the control group. Fistula was observed in one animal in the ADM group. By contrast, no related complications or other adverse situations were observed in animals treated with ADM patch modified with CBD-VEGF. The average urethra diameter was significantly smaller in the control animals than in scaffold implantation groups. Pathological examination revealed more distribution of proliferative blood vessels in the animals treated with ADM modified with CBD-VEGF. Conclusions. Overall, ADM patches modified with CBD-VEGF demonstrated an optimized tissue repair performance in a way to increase tissue angiogenesis and maintain urethral function without inducing severe inflammation and scar formation.</description><subject>Acellular Dermis - metabolism</subject><subject>Angiogenesis</subject><subject>Angiography</subject><subject>Animals</subject><subject>Binding</subject><subject>Biomedical materials</subject><subject>Bladder</subject><subject>Blood vessels</subject><subject>Care and treatment</subject><subject>Catheters</subject><subject>Cattle</subject><subject>Cell growth</subject><subject>Collagen</subject><subject>Collagen - metabolism</subject><subject>Complications</subject><subject>Disease Models, Animal</subject><subject>Dogs</subject><subject>Equipment and supplies</subject><subject>Growth factors</subject><subject>Health aspects</subject><subject>Hypospadias</subject><subject>Hypospadias - metabolism</subject><subject>Hypospadias - pathology</subject><subject>Hypospadias - surgery</subject><subject>Implantation</subject><subject>Injuries</subject><subject>Male</subject><subject>Medical imaging</subject><subject>Methods</subject><subject>Penis</subject><subject>Pharmaceuticals</subject><subject>Physiological aspects</subject><subject>Quantitative analysis</subject><subject>Reconstruction</subject><subject>Reconstructive Surgical Procedures - methods</subject><subject>Repair</subject><subject>Scaffolds</subject><subject>Skin & tissue grafts</subject><subject>Software</subject><subject>Surgery</subject><subject>Surgery, Plastic</subject><subject>Tissue engineering</subject><subject>Tissue Scaffolds</subject><subject>Tissues</subject><subject>Urethra</subject><subject>Urethra - chemistry</subject><subject>Urethra - surgery</subject><subject>Urethra - transplantation</subject><subject>Urethral diseases</subject><subject>Urinary organs</subject><subject>Vascular endothelial growth factor</subject><subject>Vascular Endothelial Growth Factor A - genetics</subject><subject>Vascular Endothelial Growth Factor A - metabolism</subject><subject>Wound healing</subject><subject>Wound Healing - drug effects</subject><issn>2314-6133</issn><issn>2314-6141</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>RHX</sourceid><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kktv1DAUhSMEolXpjjWyxAYJQv2Os0GaDm2p1AqEOmwtj3Mz8ShjFzuh9Dfwp3GYYXgs6o0t-bvn-F6fonhO8FtChDihmJITITCtOH5UHFJGeCkJJ4_3Z8YOiuOU1jgvRSSu5dPigHFZU8XIYfFjEWHoounRjUtpBPQZbPBpiKMdXPBokZxfoaEDNLPQ92NvInoPcZMLrs0Q3Xf0yQy2Q9ehca2DBt25oUPz0PdmBb48db6ZBL6cXZwj59EpmFUPaG966ddjvJ-qoU_Piiet6RMc7_ajYnF-djP_UF59vLicz65KKzAfSkaXLbOVBUPMslWCYtXWtuZCEkyhZZSKhvNaZaRtRAVWVJVSFJThjDeYsKPi3Vb3dlxuoLHgh_wWfRvdxsR7HYzT_9541-lV-KaVYJIzlgVe7QRi-DpCGvTGpWk8xkMYk6ZCiZrWhPKMvvwPXYcx-tzeL4rUiknxh1qZHrTzbci-dhLVM1lLxQXD8mFKMSEorybHN1vKxpBShHbfGMF6Co2eQqN3ocn4i7-HsYd_RyQDr7dAl__S3LmH5X4CJdvHvQ</recordid><startdate>2021</startdate><enddate>2021</enddate><creator>Wang, Yanni</creator><creator>Wang, Guannan</creator><creator>Hou, Xianglin</creator><creator>Zhao, Yannan</creator><creator>Chen, Bing</creator><creator>Dai, Jianwu</creator><creator>Sun, Ning</creator><general>Hindawi</general><general>John Wiley & Sons, Inc</general><general>Hindawi Limited</general><scope>RHU</scope><scope>RHW</scope><scope>RHX</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7QO</scope><scope>7T7</scope><scope>7TK</scope><scope>7U7</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>CWDGH</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-7963-4625</orcidid><orcidid>https://orcid.org/0000-0002-3379-9053</orcidid></search><sort><creationdate>2021</creationdate><title>Urethral Tissue Reconstruction Using the Acellular Dermal Matrix Patch Modified with Collagen-Binding VEGF in Beagle Urethral Injury Models</title><author>Wang, Yanni ; Wang, Guannan ; Hou, Xianglin ; Zhao, Yannan ; Chen, Bing ; Dai, Jianwu ; Sun, Ning</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c504t-32bf3c7cea1abf85208f9c9456102ef3225d4498c7cfd57ec577882e8a434d013</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Acellular Dermis - metabolism</topic><topic>Angiogenesis</topic><topic>Angiography</topic><topic>Animals</topic><topic>Binding</topic><topic>Biomedical materials</topic><topic>Bladder</topic><topic>Blood vessels</topic><topic>Care and treatment</topic><topic>Catheters</topic><topic>Cattle</topic><topic>Cell growth</topic><topic>Collagen</topic><topic>Collagen - metabolism</topic><topic>Complications</topic><topic>Disease Models, Animal</topic><topic>Dogs</topic><topic>Equipment and supplies</topic><topic>Growth factors</topic><topic>Health aspects</topic><topic>Hypospadias</topic><topic>Hypospadias - metabolism</topic><topic>Hypospadias - pathology</topic><topic>Hypospadias - surgery</topic><topic>Implantation</topic><topic>Injuries</topic><topic>Male</topic><topic>Medical imaging</topic><topic>Methods</topic><topic>Penis</topic><topic>Pharmaceuticals</topic><topic>Physiological aspects</topic><topic>Quantitative analysis</topic><topic>Reconstruction</topic><topic>Reconstructive Surgical Procedures - methods</topic><topic>Repair</topic><topic>Scaffolds</topic><topic>Skin & tissue grafts</topic><topic>Software</topic><topic>Surgery</topic><topic>Surgery, Plastic</topic><topic>Tissue engineering</topic><topic>Tissue Scaffolds</topic><topic>Tissues</topic><topic>Urethra</topic><topic>Urethra - chemistry</topic><topic>Urethra - surgery</topic><topic>Urethra - transplantation</topic><topic>Urethral diseases</topic><topic>Urinary organs</topic><topic>Vascular endothelial growth factor</topic><topic>Vascular Endothelial Growth Factor A - genetics</topic><topic>Vascular Endothelial Growth Factor A - metabolism</topic><topic>Wound healing</topic><topic>Wound Healing - drug effects</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wang, Yanni</creatorcontrib><creatorcontrib>Wang, Guannan</creatorcontrib><creatorcontrib>Hou, Xianglin</creatorcontrib><creatorcontrib>Zhao, Yannan</creatorcontrib><creatorcontrib>Chen, Bing</creatorcontrib><creatorcontrib>Dai, Jianwu</creatorcontrib><creatorcontrib>Sun, Ning</creatorcontrib><collection>Hindawi Publishing Complete</collection><collection>Hindawi Publishing Subscription Journals</collection><collection>Hindawi Publishing Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>Middle East & Africa Database</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>BioMed research international</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, Yanni</au><au>Wang, Guannan</au><au>Hou, Xianglin</au><au>Zhao, Yannan</au><au>Chen, Bing</au><au>Dai, Jianwu</au><au>Sun, Ning</au><au>Wang, Aijun</au><au>Aijun Wang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Urethral Tissue Reconstruction Using the Acellular Dermal Matrix Patch Modified with Collagen-Binding VEGF in Beagle Urethral Injury Models</atitle><jtitle>BioMed research international</jtitle><addtitle>Biomed Res Int</addtitle><date>2021</date><risdate>2021</risdate><volume>2021</volume><issue>1</issue><spage>5502740</spage><epage>5502740</epage><pages>5502740-5502740</pages><issn>2314-6133</issn><eissn>2314-6141</eissn><abstract>Objectives. Urethral tissue reconstruction for hypospadias is challenging for urologists. In this study, bovine acellular dermal matrix (ADM) patch loading with collagen-binding vascular endothelial growth factor (CBD-VEGF) was used to repair the urethral injury in beagles. Methods. The safety and effectiveness of the scaffold implantation were carefully evaluated by comparing among the urethral injury control group, ADM implantation group, and ADM modified with CBD-VEGF implantation group during 6 months. Urodynamic examination, urethral angiography, and pathological examination were performed to evaluate the recovery of urethral tissue. Results. Stricture, urethral diverticulum, and increased urethral closure pressure were observed in the control group. Fistula was observed in one animal in the ADM group. By contrast, no related complications or other adverse situations were observed in animals treated with ADM patch modified with CBD-VEGF. The average urethra diameter was significantly smaller in the control animals than in scaffold implantation groups. Pathological examination revealed more distribution of proliferative blood vessels in the animals treated with ADM modified with CBD-VEGF. Conclusions. Overall, ADM patches modified with CBD-VEGF demonstrated an optimized tissue repair performance in a way to increase tissue angiogenesis and maintain urethral function without inducing severe inflammation and scar formation.</abstract><cop>United States</cop><pub>Hindawi</pub><pmid>34692831</pmid><doi>10.1155/2021/5502740</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0002-7963-4625</orcidid><orcidid>https://orcid.org/0000-0002-3379-9053</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 2314-6133 |
| ispartof | BioMed research international, 2021, Vol.2021 (1), p.5502740-5502740 |
| issn | 2314-6133 2314-6141 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_8536433 |
| source | MEDLINE; PubMed Central Open Access; Wiley Online Library Open Access; PubMed Central; Alma/SFX Local Collection |
| subjects | Acellular Dermis - metabolism Angiogenesis Angiography Animals Binding Biomedical materials Bladder Blood vessels Care and treatment Catheters Cattle Cell growth Collagen Collagen - metabolism Complications Disease Models, Animal Dogs Equipment and supplies Growth factors Health aspects Hypospadias Hypospadias - metabolism Hypospadias - pathology Hypospadias - surgery Implantation Injuries Male Medical imaging Methods Penis Pharmaceuticals Physiological aspects Quantitative analysis Reconstruction Reconstructive Surgical Procedures - methods Repair Scaffolds Skin & tissue grafts Software Surgery Surgery, Plastic Tissue engineering Tissue Scaffolds Tissues Urethra Urethra - chemistry Urethra - surgery Urethra - transplantation Urethral diseases Urinary organs Vascular endothelial growth factor Vascular Endothelial Growth Factor A - genetics Vascular Endothelial Growth Factor A - metabolism Wound healing Wound Healing - drug effects |
| title | Urethral Tissue Reconstruction Using the Acellular Dermal Matrix Patch Modified with Collagen-Binding VEGF in Beagle Urethral Injury Models |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-22T06%3A15%3A45IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Urethral%20Tissue%20Reconstruction%20Using%20the%20Acellular%20Dermal%20Matrix%20Patch%20Modified%20with%20Collagen-Binding%20VEGF%20in%20Beagle%20Urethral%20Injury%20Models&rft.jtitle=BioMed%20research%20international&rft.au=Wang,%20Yanni&rft.date=2021&rft.volume=2021&rft.issue=1&rft.spage=5502740&rft.epage=5502740&rft.pages=5502740-5502740&rft.issn=2314-6133&rft.eissn=2314-6141&rft_id=info:doi/10.1155/2021/5502740&rft_dat=%3Cgale_pubme%3EA683552474%3C/gale_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2585198365&rft_id=info:pmid/34692831&rft_galeid=A683552474&rfr_iscdi=true |