Evaluation of IL-1α and IL-1β, COX-2, and iNOS mRNA Expression in Orthodontic Patients Given Chitosan Mouthwash During Treatment with Miniscrew
Chitosan is a biomaterial with antibacterial properties that may benefit from maintaining peri-miniscrew hygiene and preventing inflammation. This study aimed to evaluate the expression of inflammatory-related molecules from the gingival crevicular fluid (GCF) after treatment of 1% chitosan when com...
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| Veröffentlicht in: | Journal of International Society of Preventive & Community Dentistry 2021-09, Vol.11 (5), p.561-565 |
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| creator | Anggani, Haru Setyo Hasriati, Erlina Winiati Bachtiar, Endang |
| description | Chitosan is a biomaterial with antibacterial properties that may benefit from maintaining peri-miniscrew hygiene and preventing inflammation. This study aimed to evaluate the expression of inflammatory-related molecules from the gingival crevicular fluid (GCF) after treatment of 1% chitosan when compared with 0.2% chlorhexidine mouthwash of patients with orthodontic miniscrew.
A total of 30 subjects were divided into three groups: the first group received mouthwash containing 1% chitosan, the second group 0.2% chlorhexidine digluconate, and the control group received aquadest. The GCF was collected before and after 4 days of rinsing, and relative expressions of IL-1α and IL-1β, cyclooxygenase-2 (COX-2), and inducible nitric oxide synthase (iNOS) were evaluated by real-time qPCR.
The expression of IL-1α was the highest in chitosan-treated patients when compared with that of IL-1β in between-groups. Patients receiving chlorhexidine have the highest expression of COX-2 and iNOS when compared with the chitosan and control groups, respectively.
A mouthwash containing 1% of chitosan could suppress the expression of inflammatory mediators IL-1β, COX-2, and iNOS. |
| doi_str_mv | 10.4103/jispcd.JISPCD_163_21 |
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A total of 30 subjects were divided into three groups: the first group received mouthwash containing 1% chitosan, the second group 0.2% chlorhexidine digluconate, and the control group received aquadest. The GCF was collected before and after 4 days of rinsing, and relative expressions of IL-1α and IL-1β, cyclooxygenase-2 (COX-2), and inducible nitric oxide synthase (iNOS) were evaluated by real-time qPCR.
The expression of IL-1α was the highest in chitosan-treated patients when compared with that of IL-1β in between-groups. Patients receiving chlorhexidine have the highest expression of COX-2 and iNOS when compared with the chitosan and control groups, respectively.
A mouthwash containing 1% of chitosan could suppress the expression of inflammatory mediators IL-1β, COX-2, and iNOS.</description><identifier>ISSN: 2231-0762</identifier><identifier>EISSN: 2250-1002</identifier><identifier>DOI: 10.4103/jispcd.JISPCD_163_21</identifier><identifier>PMID: 34760801</identifier><language>eng</language><publisher>India: Medknow Publications & Media Pvt. Ltd</publisher><subject>Biomaterials ; Chitosan ; Chlorhexidine ; Cyclooxygenase-2 ; Gene expression ; Hygiene ; IL-1β ; Inflammation ; Mouthwashes ; Nitric oxide ; Nitric-oxide synthase ; Original ; Orthodontics ; Patients</subject><ispartof>Journal of International Society of Preventive & Community Dentistry, 2021-09, Vol.11 (5), p.561-565</ispartof><rights>Copyright: © 2021 Journal of International Society of Preventive and Community Dentistry.</rights><rights>2021. This article is published under (http://creativecommons.org/licenses/by-nc-sa/3.0/) (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>Copyright: © 2021 Journal of International Society of Preventive and Community Dentistry 2021</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c436t-1a4893f6c90a987d90ca903f8bbe46f2d979517b2bf7bcd14e79a0ec20291e8f3</citedby><cites>FETCH-LOGICAL-c436t-1a4893f6c90a987d90ca903f8bbe46f2d979517b2bf7bcd14e79a0ec20291e8f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8533032/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8533032/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34760801$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Anggani, Haru Setyo</creatorcontrib><creatorcontrib>Hasriati, Erlina</creatorcontrib><creatorcontrib>Winiati Bachtiar, Endang</creatorcontrib><title>Evaluation of IL-1α and IL-1β, COX-2, and iNOS mRNA Expression in Orthodontic Patients Given Chitosan Mouthwash During Treatment with Miniscrew</title><title>Journal of International Society of Preventive & Community Dentistry</title><addtitle>J Int Soc Prev Community Dent</addtitle><description>Chitosan is a biomaterial with antibacterial properties that may benefit from maintaining peri-miniscrew hygiene and preventing inflammation. This study aimed to evaluate the expression of inflammatory-related molecules from the gingival crevicular fluid (GCF) after treatment of 1% chitosan when compared with 0.2% chlorhexidine mouthwash of patients with orthodontic miniscrew.
A total of 30 subjects were divided into three groups: the first group received mouthwash containing 1% chitosan, the second group 0.2% chlorhexidine digluconate, and the control group received aquadest. The GCF was collected before and after 4 days of rinsing, and relative expressions of IL-1α and IL-1β, cyclooxygenase-2 (COX-2), and inducible nitric oxide synthase (iNOS) were evaluated by real-time qPCR.
The expression of IL-1α was the highest in chitosan-treated patients when compared with that of IL-1β in between-groups. Patients receiving chlorhexidine have the highest expression of COX-2 and iNOS when compared with the chitosan and control groups, respectively.
A mouthwash containing 1% of chitosan could suppress the expression of inflammatory mediators IL-1β, COX-2, and iNOS.</description><subject>Biomaterials</subject><subject>Chitosan</subject><subject>Chlorhexidine</subject><subject>Cyclooxygenase-2</subject><subject>Gene expression</subject><subject>Hygiene</subject><subject>IL-1β</subject><subject>Inflammation</subject><subject>Mouthwashes</subject><subject>Nitric oxide</subject><subject>Nitric-oxide synthase</subject><subject>Original</subject><subject>Orthodontics</subject><subject>Patients</subject><issn>2231-0762</issn><issn>2250-1002</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNpVUdtOGzEQtSpQQZQ_qJAlXlnqy669fkFCS0pTBYIKlfpmeb1e1lFiB9ubtJ_Bp9AP6Td1Qygq8zKjmXPOHOkA8BGj0xwj-mlm41I3p1_HtzfVhcSMSoLfgX1CCpRhhMjOZqY4Q5yRPXAY4wwNxcpccPYe7NGcM1QivA8eRys171Wy3kHfwvEkw3-eoHLNdvx9Aqvpj4ycPK_s9fQWLr5dn8PRz2UwMW5Y1sFpSJ1vvEtWw5tBy7gU4aVdGQerziYflYNXvk_dWsUOXvTBunt4F4xKiwEK1zZ18Mo6G3Uw6w9gt1XzaA5f-gH4_nl0V33JJtPLcXU-yXROWcqwyktBW6YFUqLkjUBaCUTbsq5NzlrSCC4KzGtSt7zWDc4NFwoZTRAR2JQtPQBnW91lXy9MowcnQc3lMtiFCr-kV1a-vTjbyXu_kmVBKaJkEDh-EQj-oTcxyZnvgxs8S1JwViKKeTGg8i1KBx9jMO3rB4zkJku5zVK-yXKgHf3v7pX0Lzn6F6inn3U</recordid><startdate>20210901</startdate><enddate>20210901</enddate><creator>Anggani, Haru Setyo</creator><creator>Hasriati, Erlina</creator><creator>Winiati Bachtiar, Endang</creator><general>Medknow Publications & Media Pvt. 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This study aimed to evaluate the expression of inflammatory-related molecules from the gingival crevicular fluid (GCF) after treatment of 1% chitosan when compared with 0.2% chlorhexidine mouthwash of patients with orthodontic miniscrew.
A total of 30 subjects were divided into three groups: the first group received mouthwash containing 1% chitosan, the second group 0.2% chlorhexidine digluconate, and the control group received aquadest. The GCF was collected before and after 4 days of rinsing, and relative expressions of IL-1α and IL-1β, cyclooxygenase-2 (COX-2), and inducible nitric oxide synthase (iNOS) were evaluated by real-time qPCR.
The expression of IL-1α was the highest in chitosan-treated patients when compared with that of IL-1β in between-groups. Patients receiving chlorhexidine have the highest expression of COX-2 and iNOS when compared with the chitosan and control groups, respectively.
A mouthwash containing 1% of chitosan could suppress the expression of inflammatory mediators IL-1β, COX-2, and iNOS.</abstract><cop>India</cop><pub>Medknow Publications & Media Pvt. Ltd</pub><pmid>34760801</pmid><doi>10.4103/jispcd.JISPCD_163_21</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Journal of International Society of Preventive & Community Dentistry, 2021-09, Vol.11 (5), p.561-565 |
| issn | 2231-0762 2250-1002 |
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| source | Medknow Open Access Medical Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; PubMed Central Open Access |
| subjects | Biomaterials Chitosan Chlorhexidine Cyclooxygenase-2 Gene expression Hygiene IL-1β Inflammation Mouthwashes Nitric oxide Nitric-oxide synthase Original Orthodontics Patients |
| title | Evaluation of IL-1α and IL-1β, COX-2, and iNOS mRNA Expression in Orthodontic Patients Given Chitosan Mouthwash During Treatment with Miniscrew |
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