Dynamic changes of podocytes caused by fibroblast growth factor 2 in culture
Fibroblast growth factor 2 (FGF2) augments podocyte injury, which induces glomerulosclerosis, although the mechanisms remain obscure. In this study, we investigated the effects of FGF2 on cultured podocytes with interdigitating cell processes in rats. After 48 h incubation with FGF2 dynamic changes...
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| Veröffentlicht in: | Cell and tissue research 2021-10, Vol.386 (1), p.117-126 |
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| creator | Yaoita, Eishin Nameta, Masaaki Yoshida, Yutaka Fujinaka, Hidehiko |
| description | Fibroblast growth factor 2 (FGF2) augments podocyte injury, which induces glomerulosclerosis, although the mechanisms remain obscure. In this study, we investigated the effects of FGF2 on cultured podocytes with interdigitating cell processes in rats. After 48 h incubation with FGF2 dynamic changes in the shape of primary processes and cell bodies of podocytes resulted in the loss of interdigitation, which was clearly shown by time-lapse photography. FGF2 reduced the gene expressions of constituents of the slit diaphragm, inflections of intercellular junctions positive for nephrin, and the width of the intercellular space. Immunostaining for the proliferation marker Ki-67 was rarely seen and weakly stained in the control without FGF2, whereas intensely stained cells were frequently found in the presence of FGF2. Binucleation and cell division were also observed, although no significant increase in cell number was shown. An in vitro scratch assay revealed that FGF2 enhanced migration of podocytes. These findings show that FGF2 makes podocytes to transition from the quiescent state into the cell cycle and change their morphology due to enhanced motility, and that the culture system in this study is useful for analyzing the pathological changes of podocytes in vivo. |
| doi_str_mv | 10.1007/s00441-021-03511-x |
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In this study, we investigated the effects of FGF2 on cultured podocytes with interdigitating cell processes in rats. After 48 h incubation with FGF2 dynamic changes in the shape of primary processes and cell bodies of podocytes resulted in the loss of interdigitation, which was clearly shown by time-lapse photography. FGF2 reduced the gene expressions of constituents of the slit diaphragm, inflections of intercellular junctions positive for nephrin, and the width of the intercellular space. Immunostaining for the proliferation marker Ki-67 was rarely seen and weakly stained in the control without FGF2, whereas intensely stained cells were frequently found in the presence of FGF2. Binucleation and cell division were also observed, although no significant increase in cell number was shown. An in vitro scratch assay revealed that FGF2 enhanced migration of podocytes. These findings show that FGF2 makes podocytes to transition from the quiescent state into the cell cycle and change their morphology due to enhanced motility, and that the culture system in this study is useful for analyzing the pathological changes of podocytes in vivo.</description><identifier>ISSN: 0302-766X</identifier><identifier>EISSN: 1432-0878</identifier><identifier>DOI: 10.1007/s00441-021-03511-x</identifier><identifier>PMID: 34309730</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Analysis ; Animals ; Biomedical and Life Sciences ; Biomedicine ; Cell culture ; Cell cycle ; Cell division ; Cell number ; Cells, Cultured ; Cytology ; Fibroblast growth factor 2 ; Fibroblast Growth Factor 2 - metabolism ; Fibroblast growth factors ; Fibroblasts ; Gene expression ; Growth factors ; Human Genetics ; Male ; Molecular Medicine ; Photography ; Physiological aspects ; Podocytes - metabolism ; Proteomics ; Rats ; Rats, Sprague-Dawley ; Regular ; Regular Article</subject><ispartof>Cell and tissue research, 2021-10, Vol.386 (1), p.117-126</ispartof><rights>The Author(s) 2021</rights><rights>2021. The Author(s).</rights><rights>COPYRIGHT 2021 Springer</rights><rights>The Author(s) 2021. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). 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In this study, we investigated the effects of FGF2 on cultured podocytes with interdigitating cell processes in rats. After 48 h incubation with FGF2 dynamic changes in the shape of primary processes and cell bodies of podocytes resulted in the loss of interdigitation, which was clearly shown by time-lapse photography. FGF2 reduced the gene expressions of constituents of the slit diaphragm, inflections of intercellular junctions positive for nephrin, and the width of the intercellular space. Immunostaining for the proliferation marker Ki-67 was rarely seen and weakly stained in the control without FGF2, whereas intensely stained cells were frequently found in the presence of FGF2. Binucleation and cell division were also observed, although no significant increase in cell number was shown. An in vitro scratch assay revealed that FGF2 enhanced migration of podocytes. 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Cell and tissue research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yaoita, Eishin</au><au>Nameta, Masaaki</au><au>Yoshida, Yutaka</au><au>Fujinaka, Hidehiko</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Dynamic changes of podocytes caused by fibroblast growth factor 2 in culture</atitle><jtitle>Cell and tissue research</jtitle><stitle>Cell Tissue Res</stitle><addtitle>Cell Tissue Res</addtitle><date>2021-10-01</date><risdate>2021</risdate><volume>386</volume><issue>1</issue><spage>117</spage><epage>126</epage><pages>117-126</pages><issn>0302-766X</issn><eissn>1432-0878</eissn><abstract>Fibroblast growth factor 2 (FGF2) augments podocyte injury, which induces glomerulosclerosis, although the mechanisms remain obscure. In this study, we investigated the effects of FGF2 on cultured podocytes with interdigitating cell processes in rats. After 48 h incubation with FGF2 dynamic changes in the shape of primary processes and cell bodies of podocytes resulted in the loss of interdigitation, which was clearly shown by time-lapse photography. FGF2 reduced the gene expressions of constituents of the slit diaphragm, inflections of intercellular junctions positive for nephrin, and the width of the intercellular space. Immunostaining for the proliferation marker Ki-67 was rarely seen and weakly stained in the control without FGF2, whereas intensely stained cells were frequently found in the presence of FGF2. Binucleation and cell division were also observed, although no significant increase in cell number was shown. An in vitro scratch assay revealed that FGF2 enhanced migration of podocytes. These findings show that FGF2 makes podocytes to transition from the quiescent state into the cell cycle and change their morphology due to enhanced motility, and that the culture system in this study is useful for analyzing the pathological changes of podocytes in vivo.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>34309730</pmid><doi>10.1007/s00441-021-03511-x</doi><tpages>10</tpages><orcidid>https://orcid.org/0000-0003-4051-0702</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Analysis Animals Biomedical and Life Sciences Biomedicine Cell culture Cell cycle Cell division Cell number Cells, Cultured Cytology Fibroblast growth factor 2 Fibroblast Growth Factor 2 - metabolism Fibroblast growth factors Fibroblasts Gene expression Growth factors Human Genetics Male Molecular Medicine Photography Physiological aspects Podocytes - metabolism Proteomics Rats Rats, Sprague-Dawley Regular Regular Article |
| title | Dynamic changes of podocytes caused by fibroblast growth factor 2 in culture |
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