Chinese Medicine Leptochloa chinensis Inhibits the Malignant Behaviors of Renal Cell Carcinoma 786-O Cells by Regulating the mTOR Pathway
Background. Renal cell carcinoma (RCC) is a common malignant tumor of the urinary system that seriously threatens human life and health. This study aims to explore the role of the traditional Chinese medicine Leptochloa chinensis in the pathogenesis of RCC. Meanwhile, this study also revealed the mo...
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| Veröffentlicht in: | Evidence-based complementary and alternative medicine 2021, Vol.2021, p.5122380-7 |
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| creator | Tan, Yongshun Li, Lingyun Liu, Hongyue Yu, Jiadong Wang, Qijun Lin, Qiuju |
| description | Background. Renal cell carcinoma (RCC) is a common malignant tumor of the urinary system that seriously threatens human life and health. This study aims to explore the role of the traditional Chinese medicine Leptochloa chinensis in the pathogenesis of RCC. Meanwhile, this study also revealed the molecular biological mechanism of its antitumor activity. Methods. Human RCC 786-O cells were cultured in the RPMI-1640 medium, which contains different concentrations of Leptochloa chinensis (1,000, 3,000, and 9,000 μg/ml). MTT and flow cytometry assays were used to detect the viability of 786-O cells. Transwell and wound healing assays were used to detect cell metastasis. The protein expression was observed by western blot analysis. Results. Leptochloa can inhibit cell proliferation and induce apoptosis in RCC 786-O cells. In addition, Leptochloa can weaken the migration and invasion of 786-O cells. More importantly, Leptochloa can block the mTOR pathway by inhibiting the protein expression of p-mTOR. Moreover, the high concentration of Leptochloa chinensis has a better inhibitory effect on 786-O cells. Conclusion. The traditional Chinese medicine Leptochloa chinensis inhibits the viability and metastasis of 786-O cells by blocking the mTOR pathway. |
| doi_str_mv | 10.1155/2021/5122380 |
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Renal cell carcinoma (RCC) is a common malignant tumor of the urinary system that seriously threatens human life and health. This study aims to explore the role of the traditional Chinese medicine Leptochloa chinensis in the pathogenesis of RCC. Meanwhile, this study also revealed the molecular biological mechanism of its antitumor activity. Methods. Human RCC 786-O cells were cultured in the RPMI-1640 medium, which contains different concentrations of Leptochloa chinensis (1,000, 3,000, and 9,000 μg/ml). MTT and flow cytometry assays were used to detect the viability of 786-O cells. Transwell and wound healing assays were used to detect cell metastasis. The protein expression was observed by western blot analysis. Results. Leptochloa can inhibit cell proliferation and induce apoptosis in RCC 786-O cells. In addition, Leptochloa can weaken the migration and invasion of 786-O cells. More importantly, Leptochloa can block the mTOR pathway by inhibiting the protein expression of p-mTOR. Moreover, the high concentration of Leptochloa chinensis has a better inhibitory effect on 786-O cells. Conclusion. The traditional Chinese medicine Leptochloa chinensis inhibits the viability and metastasis of 786-O cells by blocking the mTOR pathway.</description><identifier>ISSN: 1741-427X</identifier><identifier>EISSN: 1741-4288</identifier><identifier>DOI: 10.1155/2021/5122380</identifier><identifier>PMID: 34675984</identifier><language>eng</language><publisher>United States: Hindawi</publisher><subject>Antitumor activity ; Apoptosis ; Cell culture ; Cell growth ; Cell proliferation ; Drug dosages ; Flow cytometry ; Kidney cancer ; Leptochloa ; Leptochloa chinensis ; Medical research ; Medicine ; Metastases ; Metastasis ; Proteins ; Renal cell carcinoma ; Statistical analysis ; TOR protein ; Traditional Chinese medicine ; Tumors ; Wound healing</subject><ispartof>Evidence-based complementary and alternative medicine, 2021, Vol.2021, p.5122380-7</ispartof><rights>Copyright © 2021 Yongshun Tan et al.</rights><rights>Copyright © 2021 Yongshun Tan et al. This is an open access article distributed under the Creative Commons Attribution License (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. https://creativecommons.org/licenses/by/4.0</rights><rights>Copyright © 2021 Yongshun Tan et al. 2021</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c448t-f0a0ed7c8f9ee4e166d15af04fcd912a1c51a8a4b79af469cf7523d1ca11dc523</citedby><cites>FETCH-LOGICAL-c448t-f0a0ed7c8f9ee4e166d15af04fcd912a1c51a8a4b79af469cf7523d1ca11dc523</cites><orcidid>0000-0002-5948-771X ; 0000-0001-7804-6269 ; 0000-0001-7346-4840 ; 0000-0001-7307-0067 ; 0000-0001-5210-0749 ; 0000-0002-4911-1794</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8526199/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8526199/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,4010,27900,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34675984$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Khan, Nadeem</contributor><contributor>Nadeem Khan</contributor><creatorcontrib>Tan, Yongshun</creatorcontrib><creatorcontrib>Li, Lingyun</creatorcontrib><creatorcontrib>Liu, Hongyue</creatorcontrib><creatorcontrib>Yu, Jiadong</creatorcontrib><creatorcontrib>Wang, Qijun</creatorcontrib><creatorcontrib>Lin, Qiuju</creatorcontrib><title>Chinese Medicine Leptochloa chinensis Inhibits the Malignant Behaviors of Renal Cell Carcinoma 786-O Cells by Regulating the mTOR Pathway</title><title>Evidence-based complementary and alternative medicine</title><addtitle>Evid Based Complement Alternat Med</addtitle><description>Background. Renal cell carcinoma (RCC) is a common malignant tumor of the urinary system that seriously threatens human life and health. This study aims to explore the role of the traditional Chinese medicine Leptochloa chinensis in the pathogenesis of RCC. Meanwhile, this study also revealed the molecular biological mechanism of its antitumor activity. Methods. Human RCC 786-O cells were cultured in the RPMI-1640 medium, which contains different concentrations of Leptochloa chinensis (1,000, 3,000, and 9,000 μg/ml). MTT and flow cytometry assays were used to detect the viability of 786-O cells. Transwell and wound healing assays were used to detect cell metastasis. The protein expression was observed by western blot analysis. Results. Leptochloa can inhibit cell proliferation and induce apoptosis in RCC 786-O cells. In addition, Leptochloa can weaken the migration and invasion of 786-O cells. More importantly, Leptochloa can block the mTOR pathway by inhibiting the protein expression of p-mTOR. Moreover, the high concentration of Leptochloa chinensis has a better inhibitory effect on 786-O cells. Conclusion. The traditional Chinese medicine Leptochloa chinensis inhibits the viability and metastasis of 786-O cells by blocking the mTOR pathway.</description><subject>Antitumor activity</subject><subject>Apoptosis</subject><subject>Cell culture</subject><subject>Cell growth</subject><subject>Cell proliferation</subject><subject>Drug dosages</subject><subject>Flow cytometry</subject><subject>Kidney cancer</subject><subject>Leptochloa</subject><subject>Leptochloa chinensis</subject><subject>Medical research</subject><subject>Medicine</subject><subject>Metastases</subject><subject>Metastasis</subject><subject>Proteins</subject><subject>Renal cell carcinoma</subject><subject>Statistical analysis</subject><subject>TOR protein</subject><subject>Traditional Chinese medicine</subject><subject>Tumors</subject><subject>Wound healing</subject><issn>1741-427X</issn><issn>1741-4288</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>RHX</sourceid><sourceid>8G5</sourceid><sourceid>BENPR</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNp9kV1rFDEUhgdR7IfeeS0Bb4R27CSbTDI3gi5aCysrpYJ34Wwm2UmZSbZJpmV_gv_a7EcX64U3ySHn4eWcPEXxBlcfMGbsglQEXzBMyERUz4pjzCkuKRHi-aHmv46Kkxhvq4o0nPOXxdGE1pw1gh4Xv6eddTpq9F23VuUSzfQqedX1HpDa9Fy0EV25zi5siih1GYXeLh24hD7rDu6tDxF5g661gx5NdZ8PCDnLD4C4qMv59jGixTozy7GHZN1ymzTczK_RD0jdA6xfFS8M9FG_3t-nxc-vX26m38rZ_PJq-mlWKkpFKk0FlW65EqbRmmpc1y1mYCpqVNtgAlgxDALogjdgaN0owxmZtFgBxq3K5WnxcZe7GheDbpV2KUAvV8EOENbSg5VPO852cunvpWCkxk2TA97vA4K_G3VMcrBR5Q3BaT9GSZigdMIZZRl99w9668eQv2lLMYKzO56p8x2lgo8xaHMYBldy41huHMu944y__XuBA_woNQNnOyDra-HB_j_uD_pDr9s</recordid><startdate>2021</startdate><enddate>2021</enddate><creator>Tan, Yongshun</creator><creator>Li, Lingyun</creator><creator>Liu, Hongyue</creator><creator>Yu, Jiadong</creator><creator>Wang, Qijun</creator><creator>Lin, Qiuju</creator><general>Hindawi</general><general>Hindawi Limited</general><scope>RHU</scope><scope>RHW</scope><scope>RHX</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7RV</scope><scope>7T5</scope><scope>7TO</scope><scope>7X7</scope><scope>7XB</scope><scope>88G</scope><scope>8AO</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H94</scope><scope>K9.</scope><scope>KB0</scope><scope>M0S</scope><scope>M2M</scope><scope>M2O</scope><scope>MBDVC</scope><scope>NAPCQ</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PSYQQ</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-5948-771X</orcidid><orcidid>https://orcid.org/0000-0001-7804-6269</orcidid><orcidid>https://orcid.org/0000-0001-7346-4840</orcidid><orcidid>https://orcid.org/0000-0001-7307-0067</orcidid><orcidid>https://orcid.org/0000-0001-5210-0749</orcidid><orcidid>https://orcid.org/0000-0002-4911-1794</orcidid></search><sort><creationdate>2021</creationdate><title>Chinese Medicine Leptochloa chinensis Inhibits the Malignant Behaviors of Renal Cell Carcinoma 786-O Cells by Regulating the mTOR Pathway</title><author>Tan, Yongshun ; Li, Lingyun ; Liu, Hongyue ; Yu, Jiadong ; Wang, Qijun ; Lin, Qiuju</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c448t-f0a0ed7c8f9ee4e166d15af04fcd912a1c51a8a4b79af469cf7523d1ca11dc523</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Antitumor activity</topic><topic>Apoptosis</topic><topic>Cell culture</topic><topic>Cell growth</topic><topic>Cell proliferation</topic><topic>Drug dosages</topic><topic>Flow cytometry</topic><topic>Kidney cancer</topic><topic>Leptochloa</topic><topic>Leptochloa chinensis</topic><topic>Medical research</topic><topic>Medicine</topic><topic>Metastases</topic><topic>Metastasis</topic><topic>Proteins</topic><topic>Renal cell carcinoma</topic><topic>Statistical analysis</topic><topic>TOR protein</topic><topic>Traditional Chinese medicine</topic><topic>Tumors</topic><topic>Wound healing</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tan, Yongshun</creatorcontrib><creatorcontrib>Li, Lingyun</creatorcontrib><creatorcontrib>Liu, Hongyue</creatorcontrib><creatorcontrib>Yu, Jiadong</creatorcontrib><creatorcontrib>Wang, Qijun</creatorcontrib><creatorcontrib>Lin, Qiuju</creatorcontrib><collection>Hindawi Publishing Complete</collection><collection>Hindawi Publishing Subscription Journals</collection><collection>Hindawi Publishing Open Access</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Nursing & Allied Health Database</collection><collection>Immunology Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Psychology Database (Alumni)</collection><collection>ProQuest Pharma Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>ProQuest Psychology</collection><collection>Research Library</collection><collection>Research Library (Corporate)</collection><collection>Nursing & Allied Health Premium</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest One Psychology</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Evidence-based complementary and alternative medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tan, Yongshun</au><au>Li, Lingyun</au><au>Liu, Hongyue</au><au>Yu, Jiadong</au><au>Wang, Qijun</au><au>Lin, Qiuju</au><au>Khan, Nadeem</au><au>Nadeem Khan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Chinese Medicine Leptochloa chinensis Inhibits the Malignant Behaviors of Renal Cell Carcinoma 786-O Cells by Regulating the mTOR Pathway</atitle><jtitle>Evidence-based complementary and alternative medicine</jtitle><addtitle>Evid Based Complement Alternat Med</addtitle><date>2021</date><risdate>2021</risdate><volume>2021</volume><spage>5122380</spage><epage>7</epage><pages>5122380-7</pages><issn>1741-427X</issn><eissn>1741-4288</eissn><abstract>Background. Renal cell carcinoma (RCC) is a common malignant tumor of the urinary system that seriously threatens human life and health. This study aims to explore the role of the traditional Chinese medicine Leptochloa chinensis in the pathogenesis of RCC. Meanwhile, this study also revealed the molecular biological mechanism of its antitumor activity. Methods. Human RCC 786-O cells were cultured in the RPMI-1640 medium, which contains different concentrations of Leptochloa chinensis (1,000, 3,000, and 9,000 μg/ml). MTT and flow cytometry assays were used to detect the viability of 786-O cells. Transwell and wound healing assays were used to detect cell metastasis. The protein expression was observed by western blot analysis. Results. Leptochloa can inhibit cell proliferation and induce apoptosis in RCC 786-O cells. In addition, Leptochloa can weaken the migration and invasion of 786-O cells. More importantly, Leptochloa can block the mTOR pathway by inhibiting the protein expression of p-mTOR. Moreover, the high concentration of Leptochloa chinensis has a better inhibitory effect on 786-O cells. Conclusion. The traditional Chinese medicine Leptochloa chinensis inhibits the viability and metastasis of 786-O cells by blocking the mTOR pathway.</abstract><cop>United States</cop><pub>Hindawi</pub><pmid>34675984</pmid><doi>10.1155/2021/5122380</doi><tpages>7</tpages><orcidid>https://orcid.org/0000-0002-5948-771X</orcidid><orcidid>https://orcid.org/0000-0001-7804-6269</orcidid><orcidid>https://orcid.org/0000-0001-7346-4840</orcidid><orcidid>https://orcid.org/0000-0001-7307-0067</orcidid><orcidid>https://orcid.org/0000-0001-5210-0749</orcidid><orcidid>https://orcid.org/0000-0002-4911-1794</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Antitumor activity Apoptosis Cell culture Cell growth Cell proliferation Drug dosages Flow cytometry Kidney cancer Leptochloa Leptochloa chinensis Medical research Medicine Metastases Metastasis Proteins Renal cell carcinoma Statistical analysis TOR protein Traditional Chinese medicine Tumors Wound healing |
| title | Chinese Medicine Leptochloa chinensis Inhibits the Malignant Behaviors of Renal Cell Carcinoma 786-O Cells by Regulating the mTOR Pathway |
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