TROP2 modulates the progression in papillary thyroid carcinoma
Tumor-associated calcium signal transducer 2 (TROP2) is over expressed in various kinds of human cancers and plays important roles in the proliferation, invasion and metastasis of tumor cells. However, the expression and molecular mechanism of TROP2 in thyroid papillary carcinoma (PTC) are unclear....
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| Veröffentlicht in: | Journal of Cancer 2021-01, Vol.12 (22), p.6883-6893 |
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| container_title | Journal of Cancer |
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| creator | Sun, Huali Chen, Qi Liu, Weiping Liu, Yanmei Ruan, Sihan Zhu, Chumeng Ruan, Yanyun Ying, Shenpeng Lin, Peipei |
| description | Tumor-associated calcium signal transducer 2 (TROP2) is over expressed in various kinds of human cancers and plays important roles in the proliferation, invasion and metastasis of tumor cells. However, the expression and molecular mechanism of TROP2 in thyroid papillary carcinoma (PTC) are unclear.
The expressions of TROP2 in PTC and control tissue were detected by real-time reverse transcription polymerase chain reaction (RT-PCR) and immunohistochemistry. The proliferation and invasion of PTC cell lines were examined by cell cloning and transwell assays. RNA sequencing analysis and public data analysis were assessed to investigate the potential mechanisms of TROP2 in PTC. Gene correlation analysis was conducted to explore the association between TROP2 and the related gene ISG15 in patients with PTC.
The expression of TROP2 was significantly higher in PTC than control. The high expression of TROP2 protein was associated with lymph node metastasis, tumor size and capsular infiltration (P |
| doi_str_mv | 10.7150/jca.62461 |
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The expressions of TROP2 in PTC and control tissue were detected by real-time reverse transcription polymerase chain reaction (RT-PCR) and immunohistochemistry. The proliferation and invasion of PTC cell lines were examined by cell cloning and transwell assays. RNA sequencing analysis and public data analysis were assessed to investigate the potential mechanisms of TROP2 in PTC. Gene correlation analysis was conducted to explore the association between TROP2 and the related gene ISG15 in patients with PTC.
The expression of TROP2 was significantly higher in PTC than control. The high expression of TROP2 protein was associated with lymph node metastasis, tumor size and capsular infiltration (P<0.05). SiRNA-mediated TROP2 gene expression silencing can significantly inhibit proliferation and migration of PTC cells. ISG15 decreased in TROP2 siRNA PTC cells and increased in PTC patients significantly. There was a significant correlation between the expression of TROP2 and ISG15 in PTC patients. TROP2 interacted directly with ATP6V1A, CEBPA and SOX5 and then further interacted with the immune genes. TROP2 expression and tumor-infiltrating immune cells were also correlated in thyroid cancer microenvironment.
TROP2 promotes the development of PTC. TROP2 expression was correlated with ISG15 and tumor-infiltrating immune cells in thyroid cancer.</description><identifier>ISSN: 1837-9664</identifier><identifier>EISSN: 1837-9664</identifier><identifier>DOI: 10.7150/jca.62461</identifier><identifier>PMID: 34659576</identifier><language>eng</language><publisher>Australia: Ivyspring International Publisher Pty Ltd</publisher><subject>Antibodies ; Cell culture ; Gene expression ; Membranes ; Proteins ; Research Paper ; Thyroid cancer ; Tumors ; Wound healing</subject><ispartof>Journal of Cancer, 2021-01, Vol.12 (22), p.6883-6893</ispartof><rights>The author(s).</rights><rights>2021. This work is published under https://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>The author(s) 2021</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c403t-dd44447a77b80d63c36e9c4fb2b3e2212eef226ce25646e429baa386e8451c5e3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8518010/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8518010/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34659576$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sun, Huali</creatorcontrib><creatorcontrib>Chen, Qi</creatorcontrib><creatorcontrib>Liu, Weiping</creatorcontrib><creatorcontrib>Liu, Yanmei</creatorcontrib><creatorcontrib>Ruan, Sihan</creatorcontrib><creatorcontrib>Zhu, Chumeng</creatorcontrib><creatorcontrib>Ruan, Yanyun</creatorcontrib><creatorcontrib>Ying, Shenpeng</creatorcontrib><creatorcontrib>Lin, Peipei</creatorcontrib><title>TROP2 modulates the progression in papillary thyroid carcinoma</title><title>Journal of Cancer</title><addtitle>J Cancer</addtitle><description>Tumor-associated calcium signal transducer 2 (TROP2) is over expressed in various kinds of human cancers and plays important roles in the proliferation, invasion and metastasis of tumor cells. However, the expression and molecular mechanism of TROP2 in thyroid papillary carcinoma (PTC) are unclear.
The expressions of TROP2 in PTC and control tissue were detected by real-time reverse transcription polymerase chain reaction (RT-PCR) and immunohistochemistry. The proliferation and invasion of PTC cell lines were examined by cell cloning and transwell assays. RNA sequencing analysis and public data analysis were assessed to investigate the potential mechanisms of TROP2 in PTC. Gene correlation analysis was conducted to explore the association between TROP2 and the related gene ISG15 in patients with PTC.
The expression of TROP2 was significantly higher in PTC than control. The high expression of TROP2 protein was associated with lymph node metastasis, tumor size and capsular infiltration (P<0.05). SiRNA-mediated TROP2 gene expression silencing can significantly inhibit proliferation and migration of PTC cells. ISG15 decreased in TROP2 siRNA PTC cells and increased in PTC patients significantly. There was a significant correlation between the expression of TROP2 and ISG15 in PTC patients. TROP2 interacted directly with ATP6V1A, CEBPA and SOX5 and then further interacted with the immune genes. TROP2 expression and tumor-infiltrating immune cells were also correlated in thyroid cancer microenvironment.
TROP2 promotes the development of PTC. TROP2 expression was correlated with ISG15 and tumor-infiltrating immune cells in thyroid cancer.</description><subject>Antibodies</subject><subject>Cell culture</subject><subject>Gene expression</subject><subject>Membranes</subject><subject>Proteins</subject><subject>Research Paper</subject><subject>Thyroid cancer</subject><subject>Tumors</subject><subject>Wound healing</subject><issn>1837-9664</issn><issn>1837-9664</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><recordid>eNpdkVtLxDAQhYMo7rL64B-Qgi_6UM297cuCLN5gQRF9Dmk6dbO0TU1aYf-98crqvMzAfBzOzEHoiODzjAh8sTb6XFIuyQ6akpxlaSEl392aJ-gwhDWOxQqacbaPJoxLUYhMTtH86fH-gSatq8ZGDxCSYQVJ792LhxCs6xLbJb3ubdNov4nLjXe2Soz2xnau1Qdor9ZNgMPvPkPP11dPi9t0eX9zt7hcpoZjNqRVxWNlOsvKHFeSGSahMLwuacmAUkIBakqlASokl8BpUWrNcgk5F8QIYDM0_9Ltx7KFykA3eN2o3ts2-lJOW_V309mVenFvKhckxwRHgdNvAe9eRwiDam0wEM_qwI1BUZEzRrCkNKIn_9C1G30Xz4tUIaRk8ZGROvuijHcheKh_zRCsPoJRMRj1GUxkj7fd_5I_MbB3Kg6IgA</recordid><startdate>20210101</startdate><enddate>20210101</enddate><creator>Sun, Huali</creator><creator>Chen, Qi</creator><creator>Liu, Weiping</creator><creator>Liu, Yanmei</creator><creator>Ruan, Sihan</creator><creator>Zhu, Chumeng</creator><creator>Ruan, Yanyun</creator><creator>Ying, Shenpeng</creator><creator>Lin, Peipei</creator><general>Ivyspring International Publisher Pty Ltd</general><general>Ivyspring International Publisher</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>COVID</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>M0S</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20210101</creationdate><title>TROP2 modulates the progression in papillary thyroid carcinoma</title><author>Sun, Huali ; Chen, Qi ; Liu, Weiping ; Liu, Yanmei ; Ruan, Sihan ; Zhu, Chumeng ; Ruan, Yanyun ; Ying, Shenpeng ; Lin, Peipei</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c403t-dd44447a77b80d63c36e9c4fb2b3e2212eef226ce25646e429baa386e8451c5e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Antibodies</topic><topic>Cell culture</topic><topic>Gene expression</topic><topic>Membranes</topic><topic>Proteins</topic><topic>Research Paper</topic><topic>Thyroid cancer</topic><topic>Tumors</topic><topic>Wound healing</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sun, Huali</creatorcontrib><creatorcontrib>Chen, Qi</creatorcontrib><creatorcontrib>Liu, Weiping</creatorcontrib><creatorcontrib>Liu, Yanmei</creatorcontrib><creatorcontrib>Ruan, Sihan</creatorcontrib><creatorcontrib>Zhu, Chumeng</creatorcontrib><creatorcontrib>Ruan, Yanyun</creatorcontrib><creatorcontrib>Ying, Shenpeng</creatorcontrib><creatorcontrib>Lin, Peipei</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>Coronavirus Research Database</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of Cancer</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sun, Huali</au><au>Chen, Qi</au><au>Liu, Weiping</au><au>Liu, Yanmei</au><au>Ruan, Sihan</au><au>Zhu, Chumeng</au><au>Ruan, Yanyun</au><au>Ying, Shenpeng</au><au>Lin, Peipei</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>TROP2 modulates the progression in papillary thyroid carcinoma</atitle><jtitle>Journal of Cancer</jtitle><addtitle>J Cancer</addtitle><date>2021-01-01</date><risdate>2021</risdate><volume>12</volume><issue>22</issue><spage>6883</spage><epage>6893</epage><pages>6883-6893</pages><issn>1837-9664</issn><eissn>1837-9664</eissn><abstract>Tumor-associated calcium signal transducer 2 (TROP2) is over expressed in various kinds of human cancers and plays important roles in the proliferation, invasion and metastasis of tumor cells. However, the expression and molecular mechanism of TROP2 in thyroid papillary carcinoma (PTC) are unclear.
The expressions of TROP2 in PTC and control tissue were detected by real-time reverse transcription polymerase chain reaction (RT-PCR) and immunohistochemistry. The proliferation and invasion of PTC cell lines were examined by cell cloning and transwell assays. RNA sequencing analysis and public data analysis were assessed to investigate the potential mechanisms of TROP2 in PTC. Gene correlation analysis was conducted to explore the association between TROP2 and the related gene ISG15 in patients with PTC.
The expression of TROP2 was significantly higher in PTC than control. The high expression of TROP2 protein was associated with lymph node metastasis, tumor size and capsular infiltration (P<0.05). SiRNA-mediated TROP2 gene expression silencing can significantly inhibit proliferation and migration of PTC cells. ISG15 decreased in TROP2 siRNA PTC cells and increased in PTC patients significantly. There was a significant correlation between the expression of TROP2 and ISG15 in PTC patients. TROP2 interacted directly with ATP6V1A, CEBPA and SOX5 and then further interacted with the immune genes. TROP2 expression and tumor-infiltrating immune cells were also correlated in thyroid cancer microenvironment.
TROP2 promotes the development of PTC. TROP2 expression was correlated with ISG15 and tumor-infiltrating immune cells in thyroid cancer.</abstract><cop>Australia</cop><pub>Ivyspring International Publisher Pty Ltd</pub><pmid>34659576</pmid><doi>10.7150/jca.62461</doi><tpages>11</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Antibodies Cell culture Gene expression Membranes Proteins Research Paper Thyroid cancer Tumors Wound healing |
| title | TROP2 modulates the progression in papillary thyroid carcinoma |
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