Lead-Induced Motor Dysfunction Is Associated with Oxidative Stress, Proteome Modulation, and Neurodegeneration in Motor Cortex of Rats
Lead (Pb) is a toxic metal with great neurotoxic potential. The aim of this study was to investigate the effects of a long-term Pb intoxication on the global proteomic profile, oxidative biochemistry and neuronal density in motor cortex of adult rats, and the possible outcomes related to motor funct...
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| creator | Leão, Luana Ketlen Reis Bittencourt, Leonardo Oliveira Oliveira, Ana Carolina Alves Nascimento, Priscila Cunha Ferreira, Maria Karolina Martins Miranda, Giza Hellen Nonato Ferreira, Railson de Oliveira Eiró-Quirino, Luciana Puty, Bruna Dionizio, Aline Cartágenes, Sabrina Carvalho Freire, Marco Aurelio M. Buzalaf, Marília Afonso Rabelo Crespo-Lopez, Maria Elena Maia, Cristiane Socorro Ferraz Lima, Rafael Rodrigues |
| description | Lead (Pb) is a toxic metal with great neurotoxic potential. The aim of this study was to investigate the effects of a long-term Pb intoxication on the global proteomic profile, oxidative biochemistry and neuronal density in motor cortex of adult rats, and the possible outcomes related to motor functions. For this, Wistar rats received for 55 days a dose of 50 mg/Kg of Pb acetate by intragastric gavage. Then, the motor abilities were evaluated by open field and inclined plane tests. To investigate the possible oxidative biochemistry modulation, the levels of pro-oxidant parameters as lipid peroxidation and nitrites were evaluated. The global proteomic profile was evaluated by ultraefficiency liquid chromatography system coupled with mass spectrometry (UPLC/MS) followed by bioinformatic analysis. Moreover, it was evaluated the mature neuron density by anti-NeuN immunostaining. The statistical analysis was performed through Student’s t-test, considering p |
| doi_str_mv | 10.1155/2021/5595047 |
| format | Article |
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The aim of this study was to investigate the effects of a long-term Pb intoxication on the global proteomic profile, oxidative biochemistry and neuronal density in motor cortex of adult rats, and the possible outcomes related to motor functions. For this, Wistar rats received for 55 days a dose of 50 mg/Kg of Pb acetate by intragastric gavage. Then, the motor abilities were evaluated by open field and inclined plane tests. To investigate the possible oxidative biochemistry modulation, the levels of pro-oxidant parameters as lipid peroxidation and nitrites were evaluated. The global proteomic profile was evaluated by ultraefficiency liquid chromatography system coupled with mass spectrometry (UPLC/MS) followed by bioinformatic analysis. Moreover, it was evaluated the mature neuron density by anti-NeuN immunostaining. The statistical analysis was performed through Student’s t-test, considering p<0.05. We observed oxidative stress triggering by the increase in malonaldehyde and nitrite levels in motor cortex. In the proteomic analysis, the motor cortex presented alterations in proteins associated with neural functioning, morphological organization, and neurodegenerative features. In addition, it was observed a decrease in the number of mature neurons. These findings, associated with previous evidences observed in spinal cord, cerebellum, and hippocampus under the same Pb administration protocol, corroborate with the motor deficits in the rats towards Pb. Thus, we conclude that the long-term administration to Pb in young Wistar rats triggers impairments at several organizational levels, such as biochemical and morphological, which resulted in poor motor performance.</description><identifier>ISSN: 1942-0900</identifier><identifier>EISSN: 1942-0994</identifier><identifier>DOI: 10.1155/2021/5595047</identifier><identifier>PMID: 34659634</identifier><language>eng</language><publisher>United States: Hindawi</publisher><subject>Animal cognition ; Animals ; Child development ; Laboratory animals ; Lead - adverse effects ; Lead content ; Lipid peroxidation ; Male ; Motor Cortex - pathology ; Neurodegenerative Diseases - chemically induced ; Oxidative Stress ; Proteins ; Proteome - metabolism ; Protocol ; Rats ; Rats, Wistar ; Spinal cord</subject><ispartof>Oxidative medicine and cellular longevity, 2021, Vol.2021 (1), p.5595047-5595047</ispartof><rights>Copyright © 2021 Luana Ketlen Reis Leão et al.</rights><rights>Copyright © 2021 Luana Ketlen Reis Leão et al. This is an open access article distributed under the Creative Commons Attribution License (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. https://creativecommons.org/licenses/by/4.0</rights><rights>Copyright © 2021 Luana Ketlen Reis Leão et al. 2021</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c448t-2f8e331f8da03301634d717fa9e19612159867a1a3ce10dbe161fc75866a2463</citedby><cites>FETCH-LOGICAL-c448t-2f8e331f8da03301634d717fa9e19612159867a1a3ce10dbe161fc75866a2463</cites><orcidid>0000-0002-8771-9784 ; 0000-0002-1335-6853 ; 0000-0002-8687-0124 ; 0000-0001-6412-9450 ; 0000-0001-8718-9341 ; 0000-0001-6890-0695 ; 0000-0003-1486-4013 ; 0000-0002-0643-8770 ; 0000-0002-9525-2723 ; 0000-0001-5570-8530 ; 0000-0002-5985-3951 ; 0000-0003-4949-2870 ; 0000-0003-4493-7375 ; 0000-0001-9002-6279 ; 0000-0002-4489-0880 ; 0000-0002-6483-0498</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8516562/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8516562/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,4024,27923,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34659634$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Ciobica, Alin</contributor><contributor>Alin Ciobica</contributor><creatorcontrib>Leão, Luana Ketlen Reis</creatorcontrib><creatorcontrib>Bittencourt, Leonardo Oliveira</creatorcontrib><creatorcontrib>Oliveira, Ana Carolina Alves</creatorcontrib><creatorcontrib>Nascimento, Priscila Cunha</creatorcontrib><creatorcontrib>Ferreira, Maria Karolina Martins</creatorcontrib><creatorcontrib>Miranda, Giza Hellen Nonato</creatorcontrib><creatorcontrib>Ferreira, Railson de Oliveira</creatorcontrib><creatorcontrib>Eiró-Quirino, Luciana</creatorcontrib><creatorcontrib>Puty, Bruna</creatorcontrib><creatorcontrib>Dionizio, Aline</creatorcontrib><creatorcontrib>Cartágenes, Sabrina Carvalho</creatorcontrib><creatorcontrib>Freire, Marco Aurelio M.</creatorcontrib><creatorcontrib>Buzalaf, Marília Afonso Rabelo</creatorcontrib><creatorcontrib>Crespo-Lopez, Maria Elena</creatorcontrib><creatorcontrib>Maia, Cristiane Socorro Ferraz</creatorcontrib><creatorcontrib>Lima, Rafael Rodrigues</creatorcontrib><title>Lead-Induced Motor Dysfunction Is Associated with Oxidative Stress, Proteome Modulation, and Neurodegeneration in Motor Cortex of Rats</title><title>Oxidative medicine and cellular longevity</title><addtitle>Oxid Med Cell Longev</addtitle><description>Lead (Pb) is a toxic metal with great neurotoxic potential. The aim of this study was to investigate the effects of a long-term Pb intoxication on the global proteomic profile, oxidative biochemistry and neuronal density in motor cortex of adult rats, and the possible outcomes related to motor functions. For this, Wistar rats received for 55 days a dose of 50 mg/Kg of Pb acetate by intragastric gavage. Then, the motor abilities were evaluated by open field and inclined plane tests. To investigate the possible oxidative biochemistry modulation, the levels of pro-oxidant parameters as lipid peroxidation and nitrites were evaluated. The global proteomic profile was evaluated by ultraefficiency liquid chromatography system coupled with mass spectrometry (UPLC/MS) followed by bioinformatic analysis. Moreover, it was evaluated the mature neuron density by anti-NeuN immunostaining. The statistical analysis was performed through Student’s t-test, considering p<0.05. We observed oxidative stress triggering by the increase in malonaldehyde and nitrite levels in motor cortex. In the proteomic analysis, the motor cortex presented alterations in proteins associated with neural functioning, morphological organization, and neurodegenerative features. In addition, it was observed a decrease in the number of mature neurons. These findings, associated with previous evidences observed in spinal cord, cerebellum, and hippocampus under the same Pb administration protocol, corroborate with the motor deficits in the rats towards Pb. Thus, we conclude that the long-term administration to Pb in young Wistar rats triggers impairments at several organizational levels, such as biochemical and morphological, which resulted in poor motor performance.</description><subject>Animal cognition</subject><subject>Animals</subject><subject>Child development</subject><subject>Laboratory animals</subject><subject>Lead - adverse effects</subject><subject>Lead content</subject><subject>Lipid peroxidation</subject><subject>Male</subject><subject>Motor Cortex - pathology</subject><subject>Neurodegenerative Diseases - chemically induced</subject><subject>Oxidative Stress</subject><subject>Proteins</subject><subject>Proteome - metabolism</subject><subject>Protocol</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Spinal 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The aim of this study was to investigate the effects of a long-term Pb intoxication on the global proteomic profile, oxidative biochemistry and neuronal density in motor cortex of adult rats, and the possible outcomes related to motor functions. For this, Wistar rats received for 55 days a dose of 50 mg/Kg of Pb acetate by intragastric gavage. Then, the motor abilities were evaluated by open field and inclined plane tests. To investigate the possible oxidative biochemistry modulation, the levels of pro-oxidant parameters as lipid peroxidation and nitrites were evaluated. The global proteomic profile was evaluated by ultraefficiency liquid chromatography system coupled with mass spectrometry (UPLC/MS) followed by bioinformatic analysis. Moreover, it was evaluated the mature neuron density by anti-NeuN immunostaining. The statistical analysis was performed through Student’s t-test, considering p<0.05. We observed oxidative stress triggering by the increase in malonaldehyde and nitrite levels in motor cortex. In the proteomic analysis, the motor cortex presented alterations in proteins associated with neural functioning, morphological organization, and neurodegenerative features. In addition, it was observed a decrease in the number of mature neurons. These findings, associated with previous evidences observed in spinal cord, cerebellum, and hippocampus under the same Pb administration protocol, corroborate with the motor deficits in the rats towards Pb. 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| fulltext | fulltext |
| identifier | ISSN: 1942-0900 |
| ispartof | Oxidative medicine and cellular longevity, 2021, Vol.2021 (1), p.5595047-5595047 |
| issn | 1942-0900 1942-0994 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_8516562 |
| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central Open Access; Wiley Online Library (Open Access Collection); PubMed Central; Alma/SFX Local Collection |
| subjects | Animal cognition Animals Child development Laboratory animals Lead - adverse effects Lead content Lipid peroxidation Male Motor Cortex - pathology Neurodegenerative Diseases - chemically induced Oxidative Stress Proteins Proteome - metabolism Protocol Rats Rats, Wistar Spinal cord |
| title | Lead-Induced Motor Dysfunction Is Associated with Oxidative Stress, Proteome Modulation, and Neurodegeneration in Motor Cortex of Rats |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-19T01%3A54%3A33IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Lead-Induced%20Motor%20Dysfunction%20Is%20Associated%20with%20Oxidative%20Stress,%20Proteome%20Modulation,%20and%20Neurodegeneration%20in%20Motor%20Cortex%20of%20Rats&rft.jtitle=Oxidative%20medicine%20and%20cellular%20longevity&rft.au=Le%C3%A3o,%20Luana%20Ketlen%20Reis&rft.date=2021&rft.volume=2021&rft.issue=1&rft.spage=5595047&rft.epage=5595047&rft.pages=5595047-5595047&rft.issn=1942-0900&rft.eissn=1942-0994&rft_id=info:doi/10.1155/2021/5595047&rft_dat=%3Cproquest_pubme%3E2583313791%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2582650417&rft_id=info:pmid/34659634&rfr_iscdi=true |