Calcium-Silicate-Incorporated Gellan-Chitosan Induced Osteogenic Differentiation in Mesenchymal Stromal Cells
Gellan-chitosan (GC) incorporated with CS: 0% (GC-0 CS), 10% (GC-10 CS), 20% (GC-20 CS) or 40% (GC-40 CS) / was prepared using freeze-drying method to investigate its physicochemical, biocompatible, and osteoinductive properties in human bone-marrow mesenchymal stromal cells (hBMSCs). The compositio...
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| Veröffentlicht in: | Polymers 2021-09, Vol.13 (19), p.3211 |
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| creator | Genasan, Krishnamurithy Mehrali, Mohammad Veerappan, Tarini Talebian, Sepehr Malliga Raman, Murali Singh, Simmrat Swamiappan, Sasikumar Mehrali, Mehdi Kamarul, Tunku Balaji Raghavendran, Hanumantha Rao |
| description | Gellan-chitosan (GC) incorporated with CS: 0% (GC-0 CS), 10% (GC-10 CS), 20% (GC-20 CS) or 40% (GC-40 CS)
/
was prepared using freeze-drying method to investigate its physicochemical, biocompatible, and osteoinductive properties in human bone-marrow mesenchymal stromal cells (hBMSCs). The composition of different groups was reflected in physicochemical analyses performed using BET, FTIR, and XRD. The SEM micrographs revealed excellent hBMSCs attachment in GC-40 CS. The Alamar Blue assay indicated an increased proliferation and viability of seeded hBMSCs in all groups on day 21 as compared with day 0. The hBMSCs seeded in GC-40 CS indicated osteogenic differentiation based on an amplified alkaline-phosphatase release on day 7 and 14 as compared with day 0. These cells supported bone mineralization on GC-40 CS based on Alizarin-Red assay on day 21 as compared with day 7 and increased their osteogenic gene expression (RUNX2, ALP, BGLAP, BMP, and Osteonectin) on day 21. The GC-40 CS-seeded hBMSCs initiated their osteogenic differentiation on day 7 as compared with counterparts based on an increased expression of type-1 collagen and BMP2 in immunocytochemistry analysis. In conclusion, the incorporation of 40% (
/
) calcium silicate in gellan-chitosan showed osteoinduction potential in hBMSCs, making it a potential biomaterial to treat critical bone defects. |
| doi_str_mv | 10.3390/polym13193211 |
| format | Article |
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/
was prepared using freeze-drying method to investigate its physicochemical, biocompatible, and osteoinductive properties in human bone-marrow mesenchymal stromal cells (hBMSCs). The composition of different groups was reflected in physicochemical analyses performed using BET, FTIR, and XRD. The SEM micrographs revealed excellent hBMSCs attachment in GC-40 CS. The Alamar Blue assay indicated an increased proliferation and viability of seeded hBMSCs in all groups on day 21 as compared with day 0. The hBMSCs seeded in GC-40 CS indicated osteogenic differentiation based on an amplified alkaline-phosphatase release on day 7 and 14 as compared with day 0. These cells supported bone mineralization on GC-40 CS based on Alizarin-Red assay on day 21 as compared with day 7 and increased their osteogenic gene expression (RUNX2, ALP, BGLAP, BMP, and Osteonectin) on day 21. The GC-40 CS-seeded hBMSCs initiated their osteogenic differentiation on day 7 as compared with counterparts based on an increased expression of type-1 collagen and BMP2 in immunocytochemistry analysis. In conclusion, the incorporation of 40% (
/
) calcium silicate in gellan-chitosan showed osteoinduction potential in hBMSCs, making it a potential biomaterial to treat critical bone defects.</description><identifier>ISSN: 2073-4360</identifier><identifier>EISSN: 2073-4360</identifier><identifier>DOI: 10.3390/polym13193211</identifier><identifier>PMID: 34641027</identifier><language>eng</language><publisher>Switzerland: MDPI AG</publisher><subject>Alizarin ; Biocompatibility ; Biomedical materials ; Bone marrow ; Calcium silicates ; Cell growth ; Chitosan ; Differentiation (biology) ; Gene expression ; Laboratories ; Phosphatase ; Photomicrographs ; Polymers ; Scanning electron microscopy ; Tissue engineering</subject><ispartof>Polymers, 2021-09, Vol.13 (19), p.3211</ispartof><rights>2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2021 by the authors. 2021</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c415t-78281390846c41f6f3fdc49d45098ff26f526412d7e154636ee04366df24a1c83</citedby><cites>FETCH-LOGICAL-c415t-78281390846c41f6f3fdc49d45098ff26f526412d7e154636ee04366df24a1c83</cites><orcidid>0000-0002-4735-0986 ; 0000-0002-3503-3705</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8512901/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8512901/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34641027$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Genasan, Krishnamurithy</creatorcontrib><creatorcontrib>Mehrali, Mohammad</creatorcontrib><creatorcontrib>Veerappan, Tarini</creatorcontrib><creatorcontrib>Talebian, Sepehr</creatorcontrib><creatorcontrib>Malliga Raman, Murali</creatorcontrib><creatorcontrib>Singh, Simmrat</creatorcontrib><creatorcontrib>Swamiappan, Sasikumar</creatorcontrib><creatorcontrib>Mehrali, Mehdi</creatorcontrib><creatorcontrib>Kamarul, Tunku</creatorcontrib><creatorcontrib>Balaji Raghavendran, Hanumantha Rao</creatorcontrib><title>Calcium-Silicate-Incorporated Gellan-Chitosan Induced Osteogenic Differentiation in Mesenchymal Stromal Cells</title><title>Polymers</title><addtitle>Polymers (Basel)</addtitle><description>Gellan-chitosan (GC) incorporated with CS: 0% (GC-0 CS), 10% (GC-10 CS), 20% (GC-20 CS) or 40% (GC-40 CS)
/
was prepared using freeze-drying method to investigate its physicochemical, biocompatible, and osteoinductive properties in human bone-marrow mesenchymal stromal cells (hBMSCs). The composition of different groups was reflected in physicochemical analyses performed using BET, FTIR, and XRD. The SEM micrographs revealed excellent hBMSCs attachment in GC-40 CS. The Alamar Blue assay indicated an increased proliferation and viability of seeded hBMSCs in all groups on day 21 as compared with day 0. The hBMSCs seeded in GC-40 CS indicated osteogenic differentiation based on an amplified alkaline-phosphatase release on day 7 and 14 as compared with day 0. These cells supported bone mineralization on GC-40 CS based on Alizarin-Red assay on day 21 as compared with day 7 and increased their osteogenic gene expression (RUNX2, ALP, BGLAP, BMP, and Osteonectin) on day 21. The GC-40 CS-seeded hBMSCs initiated their osteogenic differentiation on day 7 as compared with counterparts based on an increased expression of type-1 collagen and BMP2 in immunocytochemistry analysis. In conclusion, the incorporation of 40% (
/
) calcium silicate in gellan-chitosan showed osteoinduction potential in hBMSCs, making it a potential biomaterial to treat critical bone defects.</description><subject>Alizarin</subject><subject>Biocompatibility</subject><subject>Biomedical materials</subject><subject>Bone marrow</subject><subject>Calcium silicates</subject><subject>Cell growth</subject><subject>Chitosan</subject><subject>Differentiation (biology)</subject><subject>Gene expression</subject><subject>Laboratories</subject><subject>Phosphatase</subject><subject>Photomicrographs</subject><subject>Polymers</subject><subject>Scanning electron microscopy</subject><subject>Tissue engineering</subject><issn>2073-4360</issn><issn>2073-4360</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNpdkc1LAzEQxYMoVqpHr7LgxctqvjabvQiyai0oPajnELNJG9lNarIr9L83rbWoucww-fGYNw-AUwQvCang1dK3qw4RVBGM0B44wrAkOSUM7v_qR-AkxneYHi0YQ-UhGBHKKIK4PAJdLVtlhy5_tq1Vstf51Ckflj6kvskmum2ly-uF7X2ULpu6ZlBpPou99nPtrMpurTE6aNdb2VvvMuuyJx21U4tVJ9vsuQ9-XeukFI_BgZFt1CfbOgav93cv9UP-OJtM65vHXFFU9HnJMUfJH6csDQwzxDSKVg0tYMWNwcwUOBnATalRQRlhWsPklDUGU4kUJ2Nw_a27HN463ai0XZCtWAbbybASXlrx98fZhZj7T8ELhCuIksDFViD4j0HHXnQ2qs0xtB-iwAVHHGJcVAk9_4e--yG4ZG9DQVRxViYq_6ZU8DEGbXbLICjWWYo_WSb-7LeDHf2THPkCCtKbkQ</recordid><startdate>20210922</startdate><enddate>20210922</enddate><creator>Genasan, Krishnamurithy</creator><creator>Mehrali, Mohammad</creator><creator>Veerappan, Tarini</creator><creator>Talebian, Sepehr</creator><creator>Malliga Raman, Murali</creator><creator>Singh, Simmrat</creator><creator>Swamiappan, Sasikumar</creator><creator>Mehrali, Mehdi</creator><creator>Kamarul, Tunku</creator><creator>Balaji Raghavendran, Hanumantha Rao</creator><general>MDPI AG</general><general>MDPI</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SR</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>HCIFZ</scope><scope>JG9</scope><scope>KB.</scope><scope>PDBOC</scope><scope>PHGZM</scope><scope>PHGZT</scope><scope>PIMPY</scope><scope>PKEHL</scope><scope>PQEST</scope><scope>PQGLB</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-4735-0986</orcidid><orcidid>https://orcid.org/0000-0002-3503-3705</orcidid></search><sort><creationdate>20210922</creationdate><title>Calcium-Silicate-Incorporated Gellan-Chitosan Induced Osteogenic Differentiation in Mesenchymal Stromal Cells</title><author>Genasan, Krishnamurithy ; Mehrali, Mohammad ; Veerappan, Tarini ; Talebian, Sepehr ; Malliga Raman, Murali ; Singh, Simmrat ; Swamiappan, Sasikumar ; Mehrali, Mehdi ; Kamarul, Tunku ; Balaji Raghavendran, Hanumantha Rao</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c415t-78281390846c41f6f3fdc49d45098ff26f526412d7e154636ee04366df24a1c83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Alizarin</topic><topic>Biocompatibility</topic><topic>Biomedical materials</topic><topic>Bone marrow</topic><topic>Calcium silicates</topic><topic>Cell growth</topic><topic>Chitosan</topic><topic>Differentiation (biology)</topic><topic>Gene expression</topic><topic>Laboratories</topic><topic>Phosphatase</topic><topic>Photomicrographs</topic><topic>Polymers</topic><topic>Scanning electron microscopy</topic><topic>Tissue engineering</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Genasan, Krishnamurithy</creatorcontrib><creatorcontrib>Mehrali, Mohammad</creatorcontrib><creatorcontrib>Veerappan, Tarini</creatorcontrib><creatorcontrib>Talebian, Sepehr</creatorcontrib><creatorcontrib>Malliga Raman, Murali</creatorcontrib><creatorcontrib>Singh, Simmrat</creatorcontrib><creatorcontrib>Swamiappan, Sasikumar</creatorcontrib><creatorcontrib>Mehrali, Mehdi</creatorcontrib><creatorcontrib>Kamarul, Tunku</creatorcontrib><creatorcontrib>Balaji Raghavendran, Hanumantha Rao</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Engineered Materials Abstracts</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>SciTech Premium Collection</collection><collection>Materials Research Database</collection><collection>Materials Science Database</collection><collection>Materials Science Collection</collection><collection>ProQuest Central (New)</collection><collection>ProQuest One Academic (New)</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Middle East (New)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Applied & Life Sciences</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Polymers</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Genasan, Krishnamurithy</au><au>Mehrali, Mohammad</au><au>Veerappan, Tarini</au><au>Talebian, Sepehr</au><au>Malliga Raman, Murali</au><au>Singh, Simmrat</au><au>Swamiappan, Sasikumar</au><au>Mehrali, Mehdi</au><au>Kamarul, Tunku</au><au>Balaji Raghavendran, Hanumantha Rao</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Calcium-Silicate-Incorporated Gellan-Chitosan Induced Osteogenic Differentiation in Mesenchymal Stromal Cells</atitle><jtitle>Polymers</jtitle><addtitle>Polymers (Basel)</addtitle><date>2021-09-22</date><risdate>2021</risdate><volume>13</volume><issue>19</issue><spage>3211</spage><pages>3211-</pages><issn>2073-4360</issn><eissn>2073-4360</eissn><abstract>Gellan-chitosan (GC) incorporated with CS: 0% (GC-0 CS), 10% (GC-10 CS), 20% (GC-20 CS) or 40% (GC-40 CS)
/
was prepared using freeze-drying method to investigate its physicochemical, biocompatible, and osteoinductive properties in human bone-marrow mesenchymal stromal cells (hBMSCs). The composition of different groups was reflected in physicochemical analyses performed using BET, FTIR, and XRD. The SEM micrographs revealed excellent hBMSCs attachment in GC-40 CS. The Alamar Blue assay indicated an increased proliferation and viability of seeded hBMSCs in all groups on day 21 as compared with day 0. The hBMSCs seeded in GC-40 CS indicated osteogenic differentiation based on an amplified alkaline-phosphatase release on day 7 and 14 as compared with day 0. These cells supported bone mineralization on GC-40 CS based on Alizarin-Red assay on day 21 as compared with day 7 and increased their osteogenic gene expression (RUNX2, ALP, BGLAP, BMP, and Osteonectin) on day 21. The GC-40 CS-seeded hBMSCs initiated their osteogenic differentiation on day 7 as compared with counterparts based on an increased expression of type-1 collagen and BMP2 in immunocytochemistry analysis. In conclusion, the incorporation of 40% (
/
) calcium silicate in gellan-chitosan showed osteoinduction potential in hBMSCs, making it a potential biomaterial to treat critical bone defects.</abstract><cop>Switzerland</cop><pub>MDPI AG</pub><pmid>34641027</pmid><doi>10.3390/polym13193211</doi><orcidid>https://orcid.org/0000-0002-4735-0986</orcidid><orcidid>https://orcid.org/0000-0002-3503-3705</orcidid><oa>free_for_read</oa></addata></record> |
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| source | MDPI - Multidisciplinary Digital Publishing Institute; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; PubMed Central Open Access |
| subjects | Alizarin Biocompatibility Biomedical materials Bone marrow Calcium silicates Cell growth Chitosan Differentiation (biology) Gene expression Laboratories Phosphatase Photomicrographs Polymers Scanning electron microscopy Tissue engineering |
| title | Calcium-Silicate-Incorporated Gellan-Chitosan Induced Osteogenic Differentiation in Mesenchymal Stromal Cells |
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