Encapsulation of Cochleates Derived from Salmonella Infantis with Biopolymers to Develop a Potential Oral Poultry Vaccine
The aim of this study was to develop and characterize Salmonellaenterica serovar Infantis (S. Infantis) cochleates protected by encapsulation technology as a potential vaccine and to determine its safety in pullets. Cochleates were encapsulated by two technologies, spray drying and ionotropic gelati...
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| Veröffentlicht in: | Polymers 2021-10, Vol.13 (19), p.3426 |
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| creator | Avendaño, Constanza Vidal, Sonia Villamizar-Sarmiento, María Gabriela Guzmán, Miguel Hidalgo, Héctor Lapierre, Lisette Valenzuela, Carolina Sáenz, Leonardo |
| description | The aim of this study was to develop and characterize Salmonellaenterica serovar Infantis (S. Infantis) cochleates protected by encapsulation technology as a potential vaccine and to determine its safety in pullets. Cochleates were encapsulated by two technologies, spray drying and ionotropic gelation at different concentrations (0–15% v/v), and were characterized by physicochemical properties, protein content and Fourier Transform Infrared Spectroscopy (FTIR). The cochleates were white liquid suspensions with tubular shapes and a protein content of 1.0–2.1 mg/mL. After encapsulation by spray drying, microparticles ranged in size from 10.4–16.9 µm, were spherical in shape, and the protein content was 0.7–1.8 mg/g. After encapsulation by ionotropic gelation, beads ranged in size from 1620–1950 µm and were spherical in shape with a protein content of 1.0–2.5 mg/g. FTIR analysis indicated that both encapsulation processes were efficient. The cochleates encapsulated by ionotropic gelation were then tested for safety in pullets. No ill effect on the health of animals was observed upon physical or postmortem examination. In conclusion, this study was the first step in developing a potential oral S. Infantis vaccine safe for poultry using a novel cochleate encapsulation technology. Future studies are needed to determine the effectiveness of the vaccine. |
| doi_str_mv | 10.3390/polym13193426 |
| format | Article |
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Infantis) cochleates protected by encapsulation technology as a potential vaccine and to determine its safety in pullets. Cochleates were encapsulated by two technologies, spray drying and ionotropic gelation at different concentrations (0–15% v/v), and were characterized by physicochemical properties, protein content and Fourier Transform Infrared Spectroscopy (FTIR). The cochleates were white liquid suspensions with tubular shapes and a protein content of 1.0–2.1 mg/mL. After encapsulation by spray drying, microparticles ranged in size from 10.4–16.9 µm, were spherical in shape, and the protein content was 0.7–1.8 mg/g. After encapsulation by ionotropic gelation, beads ranged in size from 1620–1950 µm and were spherical in shape with a protein content of 1.0–2.5 mg/g. FTIR analysis indicated that both encapsulation processes were efficient. The cochleates encapsulated by ionotropic gelation were then tested for safety in pullets. No ill effect on the health of animals was observed upon physical or postmortem examination. In conclusion, this study was the first step in developing a potential oral S. Infantis vaccine safe for poultry using a novel cochleate encapsulation technology. Future studies are needed to determine the effectiveness of the vaccine.</description><identifier>ISSN: 2073-4360</identifier><identifier>EISSN: 2073-4360</identifier><identifier>DOI: 10.3390/polym13193426</identifier><identifier>PMID: 34641241</identifier><language>eng</language><publisher>Basel: MDPI AG</publisher><subject>Bacteria ; Beads ; Biopolymers ; Encapsulation ; Fourier transforms ; Gelation ; Infrared spectroscopy ; Meat ; Microparticles ; Poultry ; Proteins ; Public health ; Safety ; Salmonella ; Sediments ; Small intestine ; Sodium ; Spray drying ; Vaccines</subject><ispartof>Polymers, 2021-10, Vol.13 (19), p.3426</ispartof><rights>2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2021 by the authors. 2021</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c392t-63c77397aeb20b4a350f2d9b2c1b58a57fbba7b46cd55fbed7572db2356794643</citedby><cites>FETCH-LOGICAL-c392t-63c77397aeb20b4a350f2d9b2c1b58a57fbba7b46cd55fbed7572db2356794643</cites><orcidid>0000-0003-1627-3452 ; 0000-0002-2264-4128 ; 0000-0001-7647-9714</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8512329/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8512329/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,724,777,781,882,27905,27906,53772,53774</link.rule.ids></links><search><creatorcontrib>Avendaño, Constanza</creatorcontrib><creatorcontrib>Vidal, Sonia</creatorcontrib><creatorcontrib>Villamizar-Sarmiento, María Gabriela</creatorcontrib><creatorcontrib>Guzmán, Miguel</creatorcontrib><creatorcontrib>Hidalgo, Héctor</creatorcontrib><creatorcontrib>Lapierre, Lisette</creatorcontrib><creatorcontrib>Valenzuela, Carolina</creatorcontrib><creatorcontrib>Sáenz, Leonardo</creatorcontrib><title>Encapsulation of Cochleates Derived from Salmonella Infantis with Biopolymers to Develop a Potential Oral Poultry Vaccine</title><title>Polymers</title><description>The aim of this study was to develop and characterize Salmonellaenterica serovar Infantis (S. Infantis) cochleates protected by encapsulation technology as a potential vaccine and to determine its safety in pullets. Cochleates were encapsulated by two technologies, spray drying and ionotropic gelation at different concentrations (0–15% v/v), and were characterized by physicochemical properties, protein content and Fourier Transform Infrared Spectroscopy (FTIR). The cochleates were white liquid suspensions with tubular shapes and a protein content of 1.0–2.1 mg/mL. After encapsulation by spray drying, microparticles ranged in size from 10.4–16.9 µm, were spherical in shape, and the protein content was 0.7–1.8 mg/g. After encapsulation by ionotropic gelation, beads ranged in size from 1620–1950 µm and were spherical in shape with a protein content of 1.0–2.5 mg/g. FTIR analysis indicated that both encapsulation processes were efficient. The cochleates encapsulated by ionotropic gelation were then tested for safety in pullets. No ill effect on the health of animals was observed upon physical or postmortem examination. In conclusion, this study was the first step in developing a potential oral S. Infantis vaccine safe for poultry using a novel cochleate encapsulation technology. Future studies are needed to determine the effectiveness of the vaccine.</description><subject>Bacteria</subject><subject>Beads</subject><subject>Biopolymers</subject><subject>Encapsulation</subject><subject>Fourier transforms</subject><subject>Gelation</subject><subject>Infrared spectroscopy</subject><subject>Meat</subject><subject>Microparticles</subject><subject>Poultry</subject><subject>Proteins</subject><subject>Public health</subject><subject>Safety</subject><subject>Salmonella</subject><subject>Sediments</subject><subject>Small intestine</subject><subject>Sodium</subject><subject>Spray drying</subject><subject>Vaccines</subject><issn>2073-4360</issn><issn>2073-4360</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><recordid>eNpdkctrHSEUxqW0NCHJsnuhm24m9TGOM5tCe5s0gUACfWxFHafX4Him6txy__uaB6HJWXgO-PPj-zwIvaPklPOBfFwg7GfK6cBb1r1Ch4xI3rS8I6__mw_QSc63pFYruo7Kt-iAt11LWUsP0f4sWr3kNejiIWKY8AbsNjhdXMZfXfI7N-IpwYy_6zBDdCFofBknHYvP-K8vW_zFw70PlzIuUB_tXIAFa3wDxVVMB3yd6nEDayhpj39pa310x-jNpEN2J4_9CP08P_uxuWiurr9dbj5fNZYPrDQdt1LyQWpnGDGt5oJMbBwMs9SIXgs5GaOlaTs7CjEZN0oh2WgYF50cakx-hD496C6rmd1oq6XqRi3JzzrtFWivnt9Ev1W_Yad6QRlnQxX48CiQ4M_qclGzz_buI6KDNSsmetoTRvq-ou9foLewpljj3VOEUsJJpZoHyibIObnpyQwl6m6v6tle-T9pw5cv</recordid><startdate>20211006</startdate><enddate>20211006</enddate><creator>Avendaño, Constanza</creator><creator>Vidal, Sonia</creator><creator>Villamizar-Sarmiento, María Gabriela</creator><creator>Guzmán, Miguel</creator><creator>Hidalgo, Héctor</creator><creator>Lapierre, Lisette</creator><creator>Valenzuela, Carolina</creator><creator>Sáenz, Leonardo</creator><general>MDPI AG</general><general>MDPI</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7SR</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>HCIFZ</scope><scope>JG9</scope><scope>KB.</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0003-1627-3452</orcidid><orcidid>https://orcid.org/0000-0002-2264-4128</orcidid><orcidid>https://orcid.org/0000-0001-7647-9714</orcidid></search><sort><creationdate>20211006</creationdate><title>Encapsulation of Cochleates Derived from Salmonella Infantis with Biopolymers to Develop a Potential Oral Poultry Vaccine</title><author>Avendaño, Constanza ; Vidal, Sonia ; Villamizar-Sarmiento, María Gabriela ; Guzmán, Miguel ; Hidalgo, Héctor ; Lapierre, Lisette ; Valenzuela, Carolina ; Sáenz, Leonardo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c392t-63c77397aeb20b4a350f2d9b2c1b58a57fbba7b46cd55fbed7572db2356794643</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Bacteria</topic><topic>Beads</topic><topic>Biopolymers</topic><topic>Encapsulation</topic><topic>Fourier transforms</topic><topic>Gelation</topic><topic>Infrared spectroscopy</topic><topic>Meat</topic><topic>Microparticles</topic><topic>Poultry</topic><topic>Proteins</topic><topic>Public health</topic><topic>Safety</topic><topic>Salmonella</topic><topic>Sediments</topic><topic>Small intestine</topic><topic>Sodium</topic><topic>Spray drying</topic><topic>Vaccines</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Avendaño, Constanza</creatorcontrib><creatorcontrib>Vidal, Sonia</creatorcontrib><creatorcontrib>Villamizar-Sarmiento, María Gabriela</creatorcontrib><creatorcontrib>Guzmán, Miguel</creatorcontrib><creatorcontrib>Hidalgo, Héctor</creatorcontrib><creatorcontrib>Lapierre, Lisette</creatorcontrib><creatorcontrib>Valenzuela, Carolina</creatorcontrib><creatorcontrib>Sáenz, Leonardo</creatorcontrib><collection>CrossRef</collection><collection>Engineered Materials Abstracts</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>SciTech Premium Collection</collection><collection>Materials Research Database</collection><collection>Materials Science Database</collection><collection>Materials Science Collection</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Polymers</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Avendaño, Constanza</au><au>Vidal, Sonia</au><au>Villamizar-Sarmiento, María Gabriela</au><au>Guzmán, Miguel</au><au>Hidalgo, Héctor</au><au>Lapierre, Lisette</au><au>Valenzuela, Carolina</au><au>Sáenz, Leonardo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Encapsulation of Cochleates Derived from Salmonella Infantis with Biopolymers to Develop a Potential Oral Poultry Vaccine</atitle><jtitle>Polymers</jtitle><date>2021-10-06</date><risdate>2021</risdate><volume>13</volume><issue>19</issue><spage>3426</spage><pages>3426-</pages><issn>2073-4360</issn><eissn>2073-4360</eissn><abstract>The aim of this study was to develop and characterize Salmonellaenterica serovar Infantis (S. Infantis) cochleates protected by encapsulation technology as a potential vaccine and to determine its safety in pullets. Cochleates were encapsulated by two technologies, spray drying and ionotropic gelation at different concentrations (0–15% v/v), and were characterized by physicochemical properties, protein content and Fourier Transform Infrared Spectroscopy (FTIR). The cochleates were white liquid suspensions with tubular shapes and a protein content of 1.0–2.1 mg/mL. After encapsulation by spray drying, microparticles ranged in size from 10.4–16.9 µm, were spherical in shape, and the protein content was 0.7–1.8 mg/g. After encapsulation by ionotropic gelation, beads ranged in size from 1620–1950 µm and were spherical in shape with a protein content of 1.0–2.5 mg/g. FTIR analysis indicated that both encapsulation processes were efficient. The cochleates encapsulated by ionotropic gelation were then tested for safety in pullets. No ill effect on the health of animals was observed upon physical or postmortem examination. In conclusion, this study was the first step in developing a potential oral S. Infantis vaccine safe for poultry using a novel cochleate encapsulation technology. Future studies are needed to determine the effectiveness of the vaccine.</abstract><cop>Basel</cop><pub>MDPI AG</pub><pmid>34641241</pmid><doi>10.3390/polym13193426</doi><orcidid>https://orcid.org/0000-0003-1627-3452</orcidid><orcidid>https://orcid.org/0000-0002-2264-4128</orcidid><orcidid>https://orcid.org/0000-0001-7647-9714</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Bacteria Beads Biopolymers Encapsulation Fourier transforms Gelation Infrared spectroscopy Meat Microparticles Poultry Proteins Public health Safety Salmonella Sediments Small intestine Sodium Spray drying Vaccines |
| title | Encapsulation of Cochleates Derived from Salmonella Infantis with Biopolymers to Develop a Potential Oral Poultry Vaccine |
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