High-resolution structural and functional deep brain imaging using adaptive optics three-photon microscopy
Multiphoton microscopy has become a powerful tool with which to visualize the morphology and function of neural cells and circuits in the intact mammalian brain. However, tissue scattering, optical aberrations and motion artifacts degrade the imaging performance at depth. Here we describe a minimall...
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| Veröffentlicht in: | Nature methods 2021-10, Vol.18 (10), p.1253-1258 |
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| creator | Streich, Lina Boffi, Juan Carlos Wang, Ling Alhalaseh, Khaleel Barbieri, Matteo Rehm, Ronja Deivasigamani, Senthilkumar Gross, Cornelius T. Agarwal, Amit Prevedel, Robert |
| description | Multiphoton microscopy has become a powerful tool with which to visualize the morphology and function of neural cells and circuits in the intact mammalian brain. However, tissue scattering, optical aberrations and motion artifacts degrade the imaging performance at depth. Here we describe a minimally invasive intravital imaging methodology based on three-photon excitation, indirect adaptive optics (AO) and active electrocardiogram gating to advance deep-tissue imaging. Our modal-based, sensorless AO approach is robust to low signal-to-noise ratios as commonly encountered in deep scattering tissues such as the mouse brain, and permits AO correction over large axial fields of view. We demonstrate near-diffraction-limited imaging of deep cortical spines and (sub)cortical dendrites up to a depth of 1.4 mm (the edge of the mouse CA1 hippocampus). In addition, we show applications to deep-layer calcium imaging of astrocytes, including fibrous astrocytes that reside in the highly scattering corpus callosum.
Three-photon microscopy in combination with adaptive optics-based aberration correction and ECG-triggered gating allows high-resolution imaging of neurons and astrocytes up to a depth of 1.4 mm in the mouse brain. |
| doi_str_mv | 10.1038/s41592-021-01257-6 |
| format | Article |
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Three-photon microscopy in combination with adaptive optics-based aberration correction and ECG-triggered gating allows high-resolution imaging of neurons and astrocytes up to a depth of 1.4 mm in the mouse brain.</description><subject>631/1647/245/2225</subject><subject>631/1647/328/2057</subject><subject>631/1647/328/2235</subject><subject>631/378/2596</subject><subject>Adaptive optics</subject><subject>Animals</subject><subject>Astrocytes</subject><subject>Astrocytes - metabolism</subject><subject>Bioinformatics</subject><subject>Biological Microscopy</subject><subject>Biological Techniques</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedical Engineering/Biotechnology</subject><subject>Brain</subject><subject>Brain research</subject><subject>Calcium imaging</subject><subject>Calcium Signaling</subject><subject>Corpus callosum</subject><subject>Cytology</subject><subject>Dendritic 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| subjects | 631/1647/245/2225 631/1647/328/2057 631/1647/328/2235 631/378/2596 Adaptive optics Animals Astrocytes Astrocytes - metabolism Bioinformatics Biological Microscopy Biological Techniques Biomedical and Life Sciences Biomedical Engineering/Biotechnology Brain Brain research Calcium imaging Calcium Signaling Corpus callosum Cytology Dendritic spines EKG Electrocardiography Female Gating Green Fluorescent Proteins High resolution Image Processing, Computer-Assisted - methods Image resolution Laser scanning microscopy Life Sciences Male Methods Mice Mice, Transgenic Microscopy Microscopy, Fluorescence, Multiphoton - methods Morphology Neuroimaging Neuroimaging - methods Neurophysiology Optics Photons Proteomics Scattering Software Structure-function relationships Thy-1 Antigens |
| title | High-resolution structural and functional deep brain imaging using adaptive optics three-photon microscopy |
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