Membrane protein extraction and purification using partially-esterified SMA polymers

Styrene maleic acid (SMA) polymers have proven to be very successful for the extraction of membrane proteins, forming SMA lipid particles (SMALPs), which maintain a lipid bilayer around the membrane protein. SMALP-encapsulated membrane proteins can be used for functional and structural studies. The...

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Veröffentlicht in:	Biochimica et biophysica acta. Biomembranes 2021-12, Vol.1863 (12), p.183758-183758, Article 183758
Hauptverfasser:	Hawkins, Olivia P., Jahromi, Christine Parisa T., Gulamhussein, Aiman A., Nestorow, Stephanie, Bahra, Taranpreet, Shelton, Christian, Owusu-Mensah, Quincy K., Mohiddin, Naadiya, O'Rourke, Hannah, Ajmal, Mariam, Byrnes, Kara, Khan, Madiha, Nahar, Nila N., Lim, Arcella, Harris, Cassandra, Healy, Hannah, Hasan, Syeda W., Ahmed, Asma, Evans, Lora, Vaitsopoulou, Afroditi, Akram, Aneel, Williams, Chris, Binding, Johanna, Thandi, Rumandeep K., Joby, Aswathy, Guest, Ashley, Tariq, Mohammad Z., Rasool, Farah, Cavanagh, Luke, Kang, Simran, Asparuhov, Biser, Jestin, Aleksandr, Dafforn, Timothy R., Simms, John, Bill, Roslyn M., Goddard, Alan D., Rothnie, Alice J.
Format:	Artikel
Sprache:	eng
Schlagworte:	Cations Cyanobacteria - chemistry Esterification Lipid Bilayers - chemistry Lipids - chemistry Maleates - chemistry Membrane Proteins - chemistry Membrane Proteins - isolation & purification Nanoparticle Polystyrenes - chemistry Protein Conformation SMALP Solubilisation Thylakoids - chemistry Thylakoids - genetics
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