The CaCIPK3 gene positively regulates drought tolerance in pepper
Drought stress is a major agricultural problem restricting the growth, development, and productivity of plants. Calcineurin B-like proteins (CBLs) and CBL-interacting protein kinases (CIPKs) significantly influence the plant response to different stresses. However, the molecular mechanisms of CBL–CI...
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| Veröffentlicht in: | Horticulture research 2021-10, Vol.8 (1), Article 216 |
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| description | Drought stress is a major agricultural problem restricting the growth, development, and productivity of plants. Calcineurin B-like proteins (CBLs) and CBL-interacting protein kinases (CIPKs) significantly influence the plant response to different stresses. However, the molecular mechanisms of CBL–CIPK in the drought stress response of pepper are still unknown. Here, the function of
CaCIPK3
in the regulation of drought stress in pepper (
Capsicum annuum
L.) was explored. Transcriptomic data and quantitative real-time PCR (qRT-PCR) analysis revealed that
CaCIPK3
participates in the response to multiple stresses. Knockdown of
CaCIPK3
in pepper increased the sensitivity to mannitol and methyl jasmonate (MeJA). Transient overexpression of
CaCIPK3
improved drought tolerance by enhancing the activities of the antioxidant system and positively regulating jasmonate (JA)-related genes. Ectopic expression of
CaCIPK3
in tomato also improved drought and MeJA resistance. As the CaCIPK3-interacting partner, CaCBL2 positively influenced drought resistance. Additionally, CaWRKY1 and CaWRKY41 directly bound the
CaCIPK3
promoter to influence its expression. This study shows that
CaCIPK3
acts as a positive regulator in drought stress resistance via the CBL–CIPK network to regulate MeJA signaling and the antioxidant defense system. |
| doi_str_mv | 10.1038/s41438-021-00651-7 |
| format | Article |
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CaCIPK3
in the regulation of drought stress in pepper (
Capsicum annuum
L.) was explored. Transcriptomic data and quantitative real-time PCR (qRT-PCR) analysis revealed that
CaCIPK3
participates in the response to multiple stresses. Knockdown of
CaCIPK3
in pepper increased the sensitivity to mannitol and methyl jasmonate (MeJA). Transient overexpression of
CaCIPK3
improved drought tolerance by enhancing the activities of the antioxidant system and positively regulating jasmonate (JA)-related genes. Ectopic expression of
CaCIPK3
in tomato also improved drought and MeJA resistance. As the CaCIPK3-interacting partner, CaCBL2 positively influenced drought resistance. Additionally, CaWRKY1 and CaWRKY41 directly bound the
CaCIPK3
promoter to influence its expression. This study shows that
CaCIPK3
acts as a positive regulator in drought stress resistance via the CBL–CIPK network to regulate MeJA signaling and the antioxidant defense system.</description><identifier>ISSN: 2662-6810</identifier><identifier>EISSN: 2052-7276</identifier><identifier>DOI: 10.1038/s41438-021-00651-7</identifier><identifier>PMID: 34593788</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>631/449/2661/2146 ; 631/449/2675 ; Agriculture ; Antioxidants ; Biomedical and Life Sciences ; Calcineurin ; Capsicum annuum ; Cbl protein ; Drought ; Drought resistance ; Ecology ; Ectopic expression ; Gene expression ; Kinases ; Life Sciences ; Mannitol ; Methyl jasmonate ; Molecular modelling ; Peppers ; Plant Breeding/Biotechnology ; Plant Genetics and Genomics ; Plant Sciences ; Protein kinase ; Proteins ; Stresses ; Tomatoes ; Vegetables</subject><ispartof>Horticulture research, 2021-10, Vol.8 (1), Article 216</ispartof><rights>The Author(s) 2021</rights><rights>The Author(s) 2021. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c483t-f4c90d6604a40c0a52146323be0bda260780c54f7d95d845fe52e0f0c5f1b5623</citedby><cites>FETCH-LOGICAL-c483t-f4c90d6604a40c0a52146323be0bda260780c54f7d95d845fe52e0f0c5f1b5623</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8484583/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8484583/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,41120,42189,51576,53791,53793</link.rule.ids></links><search><creatorcontrib>Ma, Xiao</creatorcontrib><creatorcontrib>Li, Yang</creatorcontrib><creatorcontrib>Gai, Wen-Xian</creatorcontrib><creatorcontrib>Li, Chuang</creatorcontrib><creatorcontrib>Gong, Zhen-Hui</creatorcontrib><title>The CaCIPK3 gene positively regulates drought tolerance in pepper</title><title>Horticulture research</title><addtitle>Hortic Res</addtitle><description>Drought stress is a major agricultural problem restricting the growth, development, and productivity of plants. Calcineurin B-like proteins (CBLs) and CBL-interacting protein kinases (CIPKs) significantly influence the plant response to different stresses. However, the molecular mechanisms of CBL–CIPK in the drought stress response of pepper are still unknown. Here, the function of
CaCIPK3
in the regulation of drought stress in pepper (
Capsicum annuum
L.) was explored. Transcriptomic data and quantitative real-time PCR (qRT-PCR) analysis revealed that
CaCIPK3
participates in the response to multiple stresses. Knockdown of
CaCIPK3
in pepper increased the sensitivity to mannitol and methyl jasmonate (MeJA). Transient overexpression of
CaCIPK3
improved drought tolerance by enhancing the activities of the antioxidant system and positively regulating jasmonate (JA)-related genes. Ectopic expression of
CaCIPK3
in tomato also improved drought and MeJA resistance. As the CaCIPK3-interacting partner, CaCBL2 positively influenced drought resistance. Additionally, CaWRKY1 and CaWRKY41 directly bound the
CaCIPK3
promoter to influence its expression. This study shows that
CaCIPK3
acts as a positive regulator in drought stress resistance via the CBL–CIPK network to regulate MeJA signaling and the antioxidant defense system.</description><subject>631/449/2661/2146</subject><subject>631/449/2675</subject><subject>Agriculture</subject><subject>Antioxidants</subject><subject>Biomedical and Life Sciences</subject><subject>Calcineurin</subject><subject>Capsicum annuum</subject><subject>Cbl protein</subject><subject>Drought</subject><subject>Drought resistance</subject><subject>Ecology</subject><subject>Ectopic expression</subject><subject>Gene expression</subject><subject>Kinases</subject><subject>Life Sciences</subject><subject>Mannitol</subject><subject>Methyl jasmonate</subject><subject>Molecular modelling</subject><subject>Peppers</subject><subject>Plant Breeding/Biotechnology</subject><subject>Plant Genetics and Genomics</subject><subject>Plant Sciences</subject><subject>Protein kinase</subject><subject>Proteins</subject><subject>Stresses</subject><subject>Tomatoes</subject><subject>Vegetables</subject><issn>2662-6810</issn><issn>2052-7276</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kMtKxEAQRRtRVMb5AVcB19Hqd2cjyOBjcEAXum46SSUTiUnsTgT_3tYMihtXVVTde6q4hJxSOKfAzUUQVHCTAqMpgJI01XvkmIFkqWZa7cdeKZYqQ-GILEN4AQAqBeNSH5IjLmTGtTHH5Oppi8nKrdaP9zypscNk6EMzNu_YfiQe66l1I4ak9P1Ub8dk7Fv0riswabpkwGFAf0IOKtcGXO7qgjzfXD-t7tLNw-16dbVJC2H4mFaiyKBUCoQTUICTjArFGc8R8tIxBdpAIUWly0yWRsgKJUOo4qyiuVSML8jlzB2m_BXLArvRu9YOvnl1_sP2rrF_N12ztXX_bo2IOMMj4GwH8P3bhGG0L_3ku_izZVLrjHKuTFSxWVX4PgSP1c8FCvYreTsnb2Py9jt5q6OJz6YQxV2N_hf9j-sT5kiEJg</recordid><startdate>20211001</startdate><enddate>20211001</enddate><creator>Ma, Xiao</creator><creator>Li, Yang</creator><creator>Gai, Wen-Xian</creator><creator>Li, Chuang</creator><creator>Gong, Zhen-Hui</creator><general>Nature Publishing Group UK</general><general>Oxford University Press</general><scope>C6C</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>5PM</scope></search><sort><creationdate>20211001</creationdate><title>The CaCIPK3 gene positively regulates drought tolerance in pepper</title><author>Ma, Xiao ; Li, Yang ; Gai, Wen-Xian ; Li, Chuang ; Gong, Zhen-Hui</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c483t-f4c90d6604a40c0a52146323be0bda260780c54f7d95d845fe52e0f0c5f1b5623</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>631/449/2661/2146</topic><topic>631/449/2675</topic><topic>Agriculture</topic><topic>Antioxidants</topic><topic>Biomedical and Life Sciences</topic><topic>Calcineurin</topic><topic>Capsicum annuum</topic><topic>Cbl protein</topic><topic>Drought</topic><topic>Drought resistance</topic><topic>Ecology</topic><topic>Ectopic expression</topic><topic>Gene expression</topic><topic>Kinases</topic><topic>Life Sciences</topic><topic>Mannitol</topic><topic>Methyl jasmonate</topic><topic>Molecular modelling</topic><topic>Peppers</topic><topic>Plant Breeding/Biotechnology</topic><topic>Plant Genetics and Genomics</topic><topic>Plant Sciences</topic><topic>Protein kinase</topic><topic>Proteins</topic><topic>Stresses</topic><topic>Tomatoes</topic><topic>Vegetables</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ma, Xiao</creatorcontrib><creatorcontrib>Li, Yang</creatorcontrib><creatorcontrib>Gai, Wen-Xian</creatorcontrib><creatorcontrib>Li, Chuang</creatorcontrib><creatorcontrib>Gong, Zhen-Hui</creatorcontrib><collection>Springer Nature OA Free Journals</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Biological Science Database</collection><collection>Access via ProQuest (Open Access)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Horticulture research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ma, Xiao</au><au>Li, Yang</au><au>Gai, Wen-Xian</au><au>Li, Chuang</au><au>Gong, Zhen-Hui</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The CaCIPK3 gene positively regulates drought tolerance in pepper</atitle><jtitle>Horticulture research</jtitle><stitle>Hortic Res</stitle><date>2021-10-01</date><risdate>2021</risdate><volume>8</volume><issue>1</issue><artnum>216</artnum><issn>2662-6810</issn><eissn>2052-7276</eissn><abstract>Drought stress is a major agricultural problem restricting the growth, development, and productivity of plants. Calcineurin B-like proteins (CBLs) and CBL-interacting protein kinases (CIPKs) significantly influence the plant response to different stresses. However, the molecular mechanisms of CBL–CIPK in the drought stress response of pepper are still unknown. Here, the function of
CaCIPK3
in the regulation of drought stress in pepper (
Capsicum annuum
L.) was explored. Transcriptomic data and quantitative real-time PCR (qRT-PCR) analysis revealed that
CaCIPK3
participates in the response to multiple stresses. Knockdown of
CaCIPK3
in pepper increased the sensitivity to mannitol and methyl jasmonate (MeJA). Transient overexpression of
CaCIPK3
improved drought tolerance by enhancing the activities of the antioxidant system and positively regulating jasmonate (JA)-related genes. Ectopic expression of
CaCIPK3
in tomato also improved drought and MeJA resistance. As the CaCIPK3-interacting partner, CaCBL2 positively influenced drought resistance. Additionally, CaWRKY1 and CaWRKY41 directly bound the
CaCIPK3
promoter to influence its expression. This study shows that
CaCIPK3
acts as a positive regulator in drought stress resistance via the CBL–CIPK network to regulate MeJA signaling and the antioxidant defense system.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>34593788</pmid><doi>10.1038/s41438-021-00651-7</doi><oa>free_for_read</oa></addata></record> |
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| subjects | 631/449/2661/2146 631/449/2675 Agriculture Antioxidants Biomedical and Life Sciences Calcineurin Capsicum annuum Cbl protein Drought Drought resistance Ecology Ectopic expression Gene expression Kinases Life Sciences Mannitol Methyl jasmonate Molecular modelling Peppers Plant Breeding/Biotechnology Plant Genetics and Genomics Plant Sciences Protein kinase Proteins Stresses Tomatoes Vegetables |
| title | The CaCIPK3 gene positively regulates drought tolerance in pepper |
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