Interleukin‑Iβ promotes cartilage degeneration by regulating forkhead box protein O4 and type Ⅱ collagen
Osteoarthritis (OA) is one of the most prevalent pain‑inducing and disabling diseases globally. Aging is a primary contributing factor to the progression of OA. Forkhead box protein O4 (FOXO4) is known to be involved in the cell cycle and apoptosis regulation. The aim of the present study was to inv...
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| Veröffentlicht in: | Molecular medicine reports 2021-11, Vol.24 (5), Article 813 |
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| creator | Wu, Jianxiong Zhang, Hongjun Deng, Rulin Xing, Lifeng Hu, Mingwu Fu, Xiaoling |
| description | Osteoarthritis (OA) is one of the most prevalent pain‑inducing and disabling diseases globally. Aging is a primary contributing factor to the progression of OA. Forkhead box protein O4 (FOXO4) is known to be involved in the cell cycle and apoptosis regulation. The aim of the present study was to investigate the association between FOXO4 expression and chondrocyte degeneration in rats. Chondrocytes were assigned to the control (4‑week‑old rats), natural degeneration (16‑week‑old rats) or induced degeneration (IL‑1β‑treated chondrocytes from 4‑week‑old rats) groups. Immunocytochemical analysis with β‑galactosidase staining revealed a greater number of stained cells present in the natural and induced degeneration groups than in the control group. PCR analysis indicated lower mRNA expression levels of collagen type II α1 chain (Col2α) and higher levels of FOXO4, and western blotting revealed reduced Col2α protein expression levels and significantly elevated FOXO4 levels in the natural and induced degeneration groups, compared with those in the control group. The results of the present study revealed that FOXO4 expression was altered in the natural and induced degeneration groups, and further research and exploration are needed to clarify the underlying mechanism. |
| doi_str_mv | 10.3892/mmr.2021.12453 |
| format | Article |
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Aging is a primary contributing factor to the progression of OA. Forkhead box protein O4 (FOXO4) is known to be involved in the cell cycle and apoptosis regulation. The aim of the present study was to investigate the association between FOXO4 expression and chondrocyte degeneration in rats. Chondrocytes were assigned to the control (4‑week‑old rats), natural degeneration (16‑week‑old rats) or induced degeneration (IL‑1β‑treated chondrocytes from 4‑week‑old rats) groups. Immunocytochemical analysis with β‑galactosidase staining revealed a greater number of stained cells present in the natural and induced degeneration groups than in the control group. PCR analysis indicated lower mRNA expression levels of collagen type II α1 chain (Col2α) and higher levels of FOXO4, and western blotting revealed reduced Col2α protein expression levels and significantly elevated FOXO4 levels in the natural and induced degeneration groups, compared with those in the control group. The results of the present study revealed that FOXO4 expression was altered in the natural and induced degeneration groups, and further research and exploration are needed to clarify the underlying mechanism.</description><identifier>ISSN: 1791-2997</identifier><identifier>EISSN: 1791-3004</identifier><identifier>DOI: 10.3892/mmr.2021.12453</identifier><identifier>PMID: 34549304</identifier><language>eng</language><publisher>Greece: Spandidos Publications UK Ltd</publisher><subject>Aging ; Animals ; Apoptosis ; Arthritis ; Biomarkers ; Cartilage ; Cartilage diseases ; Cartilage, Articular - metabolism ; Cartilage, Articular - pathology ; Cell cycle ; Cells, Cultured ; Chondrocytes ; Chondrocytes - metabolism ; Collagen (type II) ; Collagen Type II - genetics ; Collagen Type II - metabolism ; Degeneration ; Disease Susceptibility ; Experiments ; Forkhead protein ; Forkhead Transcription Factors - genetics ; Forkhead Transcription Factors - metabolism ; FOXO4 protein ; Gene expression ; Gene Expression Regulation ; IL-1β ; Interleukin-1beta - metabolism ; Laboratory animals ; Life sciences ; Male ; Morphology ; Osteoarthritis ; Osteoarthritis - etiology ; Osteoarthritis - metabolism ; Osteoarthritis - pathology ; Prostheses ; Proteins ; Rats ; RNA, Messenger - genetics ; RNA, Messenger - metabolism ; Western blotting ; β-Galactosidase</subject><ispartof>Molecular medicine reports, 2021-11, Vol.24 (5), Article 813</ispartof><rights>Copyright Spandidos Publications UK Ltd. 2021</rights><rights>Copyright: © Wu et al. 2021</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c418t-1b1b724b787fb2b233beabeb640a85852d0e06b8d09bd84fc10540e508b5a6b83</citedby><cites>FETCH-LOGICAL-c418t-1b1b724b787fb2b233beabeb640a85852d0e06b8d09bd84fc10540e508b5a6b83</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,777,781,882,27905,27906</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34549304$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wu, Jianxiong</creatorcontrib><creatorcontrib>Zhang, Hongjun</creatorcontrib><creatorcontrib>Deng, Rulin</creatorcontrib><creatorcontrib>Xing, Lifeng</creatorcontrib><creatorcontrib>Hu, Mingwu</creatorcontrib><creatorcontrib>Fu, Xiaoling</creatorcontrib><title>Interleukin‑Iβ promotes cartilage degeneration by regulating forkhead box protein O4 and type Ⅱ collagen</title><title>Molecular medicine reports</title><addtitle>Mol Med Rep</addtitle><description>Osteoarthritis (OA) is one of the most prevalent pain‑inducing and disabling diseases globally. Aging is a primary contributing factor to the progression of OA. Forkhead box protein O4 (FOXO4) is known to be involved in the cell cycle and apoptosis regulation. The aim of the present study was to investigate the association between FOXO4 expression and chondrocyte degeneration in rats. Chondrocytes were assigned to the control (4‑week‑old rats), natural degeneration (16‑week‑old rats) or induced degeneration (IL‑1β‑treated chondrocytes from 4‑week‑old rats) groups. Immunocytochemical analysis with β‑galactosidase staining revealed a greater number of stained cells present in the natural and induced degeneration groups than in the control group. PCR analysis indicated lower mRNA expression levels of collagen type II α1 chain (Col2α) and higher levels of FOXO4, and western blotting revealed reduced Col2α protein expression levels and significantly elevated FOXO4 levels in the natural and induced degeneration groups, compared with those in the control group. The results of the present study revealed that FOXO4 expression was altered in the natural and induced degeneration groups, and further research and exploration are needed to clarify the underlying mechanism.</description><subject>Aging</subject><subject>Animals</subject><subject>Apoptosis</subject><subject>Arthritis</subject><subject>Biomarkers</subject><subject>Cartilage</subject><subject>Cartilage diseases</subject><subject>Cartilage, Articular - metabolism</subject><subject>Cartilage, Articular - pathology</subject><subject>Cell cycle</subject><subject>Cells, Cultured</subject><subject>Chondrocytes</subject><subject>Chondrocytes - metabolism</subject><subject>Collagen (type II)</subject><subject>Collagen Type II - genetics</subject><subject>Collagen Type II - metabolism</subject><subject>Degeneration</subject><subject>Disease Susceptibility</subject><subject>Experiments</subject><subject>Forkhead protein</subject><subject>Forkhead Transcription Factors - genetics</subject><subject>Forkhead Transcription Factors - metabolism</subject><subject>FOXO4 protein</subject><subject>Gene expression</subject><subject>Gene Expression Regulation</subject><subject>IL-1β</subject><subject>Interleukin-1beta - metabolism</subject><subject>Laboratory animals</subject><subject>Life sciences</subject><subject>Male</subject><subject>Morphology</subject><subject>Osteoarthritis</subject><subject>Osteoarthritis - etiology</subject><subject>Osteoarthritis - metabolism</subject><subject>Osteoarthritis - pathology</subject><subject>Prostheses</subject><subject>Proteins</subject><subject>Rats</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><subject>Western blotting</subject><subject>β-Galactosidase</subject><issn>1791-2997</issn><issn>1791-3004</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNpdkT1uFDEYhi0EIiHQUiJLNDS78e_abpBQBGGlSGmgtuyZbyaTzNiLPROxHU0OkBuQK3AEDsAhchI8yRIBlf-e75VfPQi9pGTJtWGHw5CWjDC6pExI_gjtU2XoghMiHu_2zBi1h57lfE7ISjJpnqI9LqQwnIh9FNZhhNTDdNGF22_X618_8CbFIY6QceXS2PWuBVxDCwGSG7sYsN_iBO3Ul1NocRPTxRm4Gvv4dR4doQv4VGAXajxuN_Dz--3VDa5iPweF5-hJ4_oML3brAfr84f2no4-Lk9Pj9dG7k0UlqB4X1FOvmPBKq8Yzzzj34Dz4lSBOSy1ZTYCsvK6J8bUWTUWJFAQk0V66cs8P0Nv73M3kB6grCGNyvd2kbnBpa6Pr7L8voTuzbby0WihFlSgBb3YBKX6ZII926HIFpUWAOGXLpJJcMaJMQV__h57HKYVSb6a04YxRVajlPVWlmHOC5uEzlNhZpS0q7azS3qksA6_-rvCA_3HHfwMcRZ-6</recordid><startdate>20211101</startdate><enddate>20211101</enddate><creator>Wu, Jianxiong</creator><creator>Zhang, Hongjun</creator><creator>Deng, Rulin</creator><creator>Xing, Lifeng</creator><creator>Hu, Mingwu</creator><creator>Fu, Xiaoling</creator><general>Spandidos Publications UK Ltd</general><general>D.A. 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Aging is a primary contributing factor to the progression of OA. Forkhead box protein O4 (FOXO4) is known to be involved in the cell cycle and apoptosis regulation. The aim of the present study was to investigate the association between FOXO4 expression and chondrocyte degeneration in rats. Chondrocytes were assigned to the control (4‑week‑old rats), natural degeneration (16‑week‑old rats) or induced degeneration (IL‑1β‑treated chondrocytes from 4‑week‑old rats) groups. Immunocytochemical analysis with β‑galactosidase staining revealed a greater number of stained cells present in the natural and induced degeneration groups than in the control group. PCR analysis indicated lower mRNA expression levels of collagen type II α1 chain (Col2α) and higher levels of FOXO4, and western blotting revealed reduced Col2α protein expression levels and significantly elevated FOXO4 levels in the natural and induced degeneration groups, compared with those in the control group. The results of the present study revealed that FOXO4 expression was altered in the natural and induced degeneration groups, and further research and exploration are needed to clarify the underlying mechanism.</abstract><cop>Greece</cop><pub>Spandidos Publications UK Ltd</pub><pmid>34549304</pmid><doi>10.3892/mmr.2021.12453</doi><oa>free_for_read</oa></addata></record> |
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| source | Spandidos Publications Journals; MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Aging Animals Apoptosis Arthritis Biomarkers Cartilage Cartilage diseases Cartilage, Articular - metabolism Cartilage, Articular - pathology Cell cycle Cells, Cultured Chondrocytes Chondrocytes - metabolism Collagen (type II) Collagen Type II - genetics Collagen Type II - metabolism Degeneration Disease Susceptibility Experiments Forkhead protein Forkhead Transcription Factors - genetics Forkhead Transcription Factors - metabolism FOXO4 protein Gene expression Gene Expression Regulation IL-1β Interleukin-1beta - metabolism Laboratory animals Life sciences Male Morphology Osteoarthritis Osteoarthritis - etiology Osteoarthritis - metabolism Osteoarthritis - pathology Prostheses Proteins Rats RNA, Messenger - genetics RNA, Messenger - metabolism Western blotting β-Galactosidase |
| title | Interleukin‑Iβ promotes cartilage degeneration by regulating forkhead box protein O4 and type Ⅱ collagen |
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