Properties of Degradable Polyhydroxyalkanoates (PHAs) Synthesized by a New Strain, Cupriavidus necator IBP/SFU-1, from Various Carbon Sources
The bacterial strain isolated from soil was identified as Cupriavidus necator IBP/SFU-1 and investigated as a PHA producer. The strain was found to be able to grow and synthesize PHAs under autotrophic conditions and showed a broad organotrophic potential towards different carbon sources: sugars, gl...
Gespeichert in:
| Veröffentlicht in: | Polymers 2021-09, Vol.13 (18), p.3142 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | |
|---|---|
| container_issue | 18 |
| container_start_page | 3142 |
| container_title | Polymers |
| container_volume | 13 |
| creator | Zhila, Natalia O. Sapozhnikova, Kristina Yu Kiselev, Evgeniy G. Vasiliev, Alexander D. Nemtsev, Ivan V. Shishatskaya, Ekaterina I. Volova, Tatiana G. |
| description | The bacterial strain isolated from soil was identified as Cupriavidus necator IBP/SFU-1 and investigated as a PHA producer. The strain was found to be able to grow and synthesize PHAs under autotrophic conditions and showed a broad organotrophic potential towards different carbon sources: sugars, glycerol, fatty acids, and plant oils. The highest cell concentrations (7–8 g/L) and PHA contents were produced from oleic acid (78%), fructose, glucose, and palm oil (over 80%). The type of the carbon source influenced the PHA chemical composition and properties: when grown on oleic acid, the strain synthesized the P(3HB-co-3HV) copolymer; on plant oils, the P(3HB-co-3HV-co-3HHx) terpolymer, and on the other substrates, the P(3HB) homopolymer. The type of the carbon source influenced molecular-weight properties of PHAs: P(3HB) synthesized under autotrophic growth conditions, from CO2, had the highest number-average (290 ± 15 kDa) and weight-average (850 ± 25 kDa) molecular weights and the lowest polydispersity (2.9 ± 0.2); polymers synthesized from organic carbon sources showed increased polydispersity and reduced molecular weight. The carbon source was not found to affect the degree of crystallinity and thermal properties of the PHAs. The type of the carbon source determined not only PHA composition and molecular weight but also surface microstructure and porosity of the polymer films. The new strain can be recommended as a promising P(3HB) producer from palm oil, oleic acid, and sugars (fructose and glucose) and as a producer of P(3HB-co-3HV) from oleic acid and P(3HB-co-3HV-co-3HHx) from palm oil. |
| doi_str_mv | 10.3390/polym13183142 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_8468435</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2576482637</sourcerecordid><originalsourceid>FETCH-LOGICAL-c392t-8c05746fe947e4790fa18a00bc9662ae753f0f445937907ac0cdc67a90e9dec73</originalsourceid><addsrcrecordid>eNpdkU9r3DAQxU1paUKaY--CXlKIE8mSJetSSLf5ByFd2KZXMZbHWaW2tZXsNO536HeuloSSdC4z8H483mOy7D2jR5xrerzx3dwzzirORPEq2y2o4rngkr5-du9k-zHe0TSilJKpt9kOF6WqqCh2sz_L4DcYRoeR-JZ8wdsADdQdkmXyXs9N8A8zdD9g8DAm5mB5cRI_ktU8jGuM7jc2pJ4JkGv8RVZjADccksW0CQ7uXTNFMqCF0Qdy-Xl5vDq7ydkhaYPvyXcIzid9AaH2A1n5KViM77I3LXQR95_2XnZzdvptcZFffT2_XJxc5ZbrYswrS0slZItaKBRK0xZYBZTWVktZAKqSt7QVotQ8iQostY2VCjRF3aBVfC_79Oi7meoeG4tDit6ZFLuHMBsPzrxUBrc2t_7eVEJWgpfJ4ODJIPifE8bR9C5a7DoYMNUyRamUEFqzLfrhP_QulR1SvS0lRVVIvk2UP1I2-BgDtv_CMGq2vzYvfs3_ApIPnNE</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2576482637</pqid></control><display><type>article</type><title>Properties of Degradable Polyhydroxyalkanoates (PHAs) Synthesized by a New Strain, Cupriavidus necator IBP/SFU-1, from Various Carbon Sources</title><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>PubMed Central Open Access</source><source>MDPI - Multidisciplinary Digital Publishing Institute</source><source>PubMed Central</source><creator>Zhila, Natalia O. ; Sapozhnikova, Kristina Yu ; Kiselev, Evgeniy G. ; Vasiliev, Alexander D. ; Nemtsev, Ivan V. ; Shishatskaya, Ekaterina I. ; Volova, Tatiana G.</creator><creatorcontrib>Zhila, Natalia O. ; Sapozhnikova, Kristina Yu ; Kiselev, Evgeniy G. ; Vasiliev, Alexander D. ; Nemtsev, Ivan V. ; Shishatskaya, Ekaterina I. ; Volova, Tatiana G.</creatorcontrib><description>The bacterial strain isolated from soil was identified as Cupriavidus necator IBP/SFU-1 and investigated as a PHA producer. The strain was found to be able to grow and synthesize PHAs under autotrophic conditions and showed a broad organotrophic potential towards different carbon sources: sugars, glycerol, fatty acids, and plant oils. The highest cell concentrations (7–8 g/L) and PHA contents were produced from oleic acid (78%), fructose, glucose, and palm oil (over 80%). The type of the carbon source influenced the PHA chemical composition and properties: when grown on oleic acid, the strain synthesized the P(3HB-co-3HV) copolymer; on plant oils, the P(3HB-co-3HV-co-3HHx) terpolymer, and on the other substrates, the P(3HB) homopolymer. The type of the carbon source influenced molecular-weight properties of PHAs: P(3HB) synthesized under autotrophic growth conditions, from CO2, had the highest number-average (290 ± 15 kDa) and weight-average (850 ± 25 kDa) molecular weights and the lowest polydispersity (2.9 ± 0.2); polymers synthesized from organic carbon sources showed increased polydispersity and reduced molecular weight. The carbon source was not found to affect the degree of crystallinity and thermal properties of the PHAs. The type of the carbon source determined not only PHA composition and molecular weight but also surface microstructure and porosity of the polymer films. The new strain can be recommended as a promising P(3HB) producer from palm oil, oleic acid, and sugars (fructose and glucose) and as a producer of P(3HB-co-3HV) from oleic acid and P(3HB-co-3HV-co-3HHx) from palm oil.</description><identifier>ISSN: 2073-4360</identifier><identifier>EISSN: 2073-4360</identifier><identifier>DOI: 10.3390/polym13183142</identifier><identifier>PMID: 34578042</identifier><language>eng</language><publisher>Basel: MDPI AG</publisher><subject>Biomass ; Carbon ; Chemical composition ; Chromatography ; Copolymers ; Degree of crystallinity ; Fatty acids ; Fermentation ; Fructose ; Glucose ; Glycerol ; Lipids ; Mass spectrometry ; Microorganisms ; Microscopy ; Molecular weight ; Oleic acid ; Organic carbon ; Palm oil ; Phylogenetics ; Polydispersity ; Polyhydroxyalkanoates ; Polymer films ; Polymers ; Proteins ; Scientific imaging ; Software ; Soil investigations ; Soil porosity ; Substrates ; Sugar ; Terpolymers ; Thermodynamic properties ; Weight reduction</subject><ispartof>Polymers, 2021-09, Vol.13 (18), p.3142</ispartof><rights>2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2021 by the authors. 2021</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c392t-8c05746fe947e4790fa18a00bc9662ae753f0f445937907ac0cdc67a90e9dec73</citedby><cites>FETCH-LOGICAL-c392t-8c05746fe947e4790fa18a00bc9662ae753f0f445937907ac0cdc67a90e9dec73</cites><orcidid>0000-0001-6054-4200 ; 0000-0001-8739-4062 ; 0000-0003-4472-7087 ; 0000-0002-4222-3099</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8468435/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8468435/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,27903,27904,53769,53771</link.rule.ids></links><search><creatorcontrib>Zhila, Natalia O.</creatorcontrib><creatorcontrib>Sapozhnikova, Kristina Yu</creatorcontrib><creatorcontrib>Kiselev, Evgeniy G.</creatorcontrib><creatorcontrib>Vasiliev, Alexander D.</creatorcontrib><creatorcontrib>Nemtsev, Ivan V.</creatorcontrib><creatorcontrib>Shishatskaya, Ekaterina I.</creatorcontrib><creatorcontrib>Volova, Tatiana G.</creatorcontrib><title>Properties of Degradable Polyhydroxyalkanoates (PHAs) Synthesized by a New Strain, Cupriavidus necator IBP/SFU-1, from Various Carbon Sources</title><title>Polymers</title><description>The bacterial strain isolated from soil was identified as Cupriavidus necator IBP/SFU-1 and investigated as a PHA producer. The strain was found to be able to grow and synthesize PHAs under autotrophic conditions and showed a broad organotrophic potential towards different carbon sources: sugars, glycerol, fatty acids, and plant oils. The highest cell concentrations (7–8 g/L) and PHA contents were produced from oleic acid (78%), fructose, glucose, and palm oil (over 80%). The type of the carbon source influenced the PHA chemical composition and properties: when grown on oleic acid, the strain synthesized the P(3HB-co-3HV) copolymer; on plant oils, the P(3HB-co-3HV-co-3HHx) terpolymer, and on the other substrates, the P(3HB) homopolymer. The type of the carbon source influenced molecular-weight properties of PHAs: P(3HB) synthesized under autotrophic growth conditions, from CO2, had the highest number-average (290 ± 15 kDa) and weight-average (850 ± 25 kDa) molecular weights and the lowest polydispersity (2.9 ± 0.2); polymers synthesized from organic carbon sources showed increased polydispersity and reduced molecular weight. The carbon source was not found to affect the degree of crystallinity and thermal properties of the PHAs. The type of the carbon source determined not only PHA composition and molecular weight but also surface microstructure and porosity of the polymer films. The new strain can be recommended as a promising P(3HB) producer from palm oil, oleic acid, and sugars (fructose and glucose) and as a producer of P(3HB-co-3HV) from oleic acid and P(3HB-co-3HV-co-3HHx) from palm oil.</description><subject>Biomass</subject><subject>Carbon</subject><subject>Chemical composition</subject><subject>Chromatography</subject><subject>Copolymers</subject><subject>Degree of crystallinity</subject><subject>Fatty acids</subject><subject>Fermentation</subject><subject>Fructose</subject><subject>Glucose</subject><subject>Glycerol</subject><subject>Lipids</subject><subject>Mass spectrometry</subject><subject>Microorganisms</subject><subject>Microscopy</subject><subject>Molecular weight</subject><subject>Oleic acid</subject><subject>Organic carbon</subject><subject>Palm oil</subject><subject>Phylogenetics</subject><subject>Polydispersity</subject><subject>Polyhydroxyalkanoates</subject><subject>Polymer films</subject><subject>Polymers</subject><subject>Proteins</subject><subject>Scientific imaging</subject><subject>Software</subject><subject>Soil investigations</subject><subject>Soil porosity</subject><subject>Substrates</subject><subject>Sugar</subject><subject>Terpolymers</subject><subject>Thermodynamic properties</subject><subject>Weight reduction</subject><issn>2073-4360</issn><issn>2073-4360</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><recordid>eNpdkU9r3DAQxU1paUKaY--CXlKIE8mSJetSSLf5ByFd2KZXMZbHWaW2tZXsNO536HeuloSSdC4z8H483mOy7D2jR5xrerzx3dwzzirORPEq2y2o4rngkr5-du9k-zHe0TSilJKpt9kOF6WqqCh2sz_L4DcYRoeR-JZ8wdsADdQdkmXyXs9N8A8zdD9g8DAm5mB5cRI_ktU8jGuM7jc2pJ4JkGv8RVZjADccksW0CQ7uXTNFMqCF0Qdy-Xl5vDq7ydkhaYPvyXcIzid9AaH2A1n5KViM77I3LXQR95_2XnZzdvptcZFffT2_XJxc5ZbrYswrS0slZItaKBRK0xZYBZTWVktZAKqSt7QVotQ8iQostY2VCjRF3aBVfC_79Oi7meoeG4tDit6ZFLuHMBsPzrxUBrc2t_7eVEJWgpfJ4ODJIPifE8bR9C5a7DoYMNUyRamUEFqzLfrhP_QulR1SvS0lRVVIvk2UP1I2-BgDtv_CMGq2vzYvfs3_ApIPnNE</recordid><startdate>20210917</startdate><enddate>20210917</enddate><creator>Zhila, Natalia O.</creator><creator>Sapozhnikova, Kristina Yu</creator><creator>Kiselev, Evgeniy G.</creator><creator>Vasiliev, Alexander D.</creator><creator>Nemtsev, Ivan V.</creator><creator>Shishatskaya, Ekaterina I.</creator><creator>Volova, Tatiana G.</creator><general>MDPI AG</general><general>MDPI</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7SR</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>HCIFZ</scope><scope>JG9</scope><scope>KB.</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0001-6054-4200</orcidid><orcidid>https://orcid.org/0000-0001-8739-4062</orcidid><orcidid>https://orcid.org/0000-0003-4472-7087</orcidid><orcidid>https://orcid.org/0000-0002-4222-3099</orcidid></search><sort><creationdate>20210917</creationdate><title>Properties of Degradable Polyhydroxyalkanoates (PHAs) Synthesized by a New Strain, Cupriavidus necator IBP/SFU-1, from Various Carbon Sources</title><author>Zhila, Natalia O. ; Sapozhnikova, Kristina Yu ; Kiselev, Evgeniy G. ; Vasiliev, Alexander D. ; Nemtsev, Ivan V. ; Shishatskaya, Ekaterina I. ; Volova, Tatiana G.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c392t-8c05746fe947e4790fa18a00bc9662ae753f0f445937907ac0cdc67a90e9dec73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Biomass</topic><topic>Carbon</topic><topic>Chemical composition</topic><topic>Chromatography</topic><topic>Copolymers</topic><topic>Degree of crystallinity</topic><topic>Fatty acids</topic><topic>Fermentation</topic><topic>Fructose</topic><topic>Glucose</topic><topic>Glycerol</topic><topic>Lipids</topic><topic>Mass spectrometry</topic><topic>Microorganisms</topic><topic>Microscopy</topic><topic>Molecular weight</topic><topic>Oleic acid</topic><topic>Organic carbon</topic><topic>Palm oil</topic><topic>Phylogenetics</topic><topic>Polydispersity</topic><topic>Polyhydroxyalkanoates</topic><topic>Polymer films</topic><topic>Polymers</topic><topic>Proteins</topic><topic>Scientific imaging</topic><topic>Software</topic><topic>Soil investigations</topic><topic>Soil porosity</topic><topic>Substrates</topic><topic>Sugar</topic><topic>Terpolymers</topic><topic>Thermodynamic properties</topic><topic>Weight reduction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhila, Natalia O.</creatorcontrib><creatorcontrib>Sapozhnikova, Kristina Yu</creatorcontrib><creatorcontrib>Kiselev, Evgeniy G.</creatorcontrib><creatorcontrib>Vasiliev, Alexander D.</creatorcontrib><creatorcontrib>Nemtsev, Ivan V.</creatorcontrib><creatorcontrib>Shishatskaya, Ekaterina I.</creatorcontrib><creatorcontrib>Volova, Tatiana G.</creatorcontrib><collection>CrossRef</collection><collection>Engineered Materials Abstracts</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>SciTech Premium Collection</collection><collection>Materials Research Database</collection><collection>Materials Science Database</collection><collection>Materials Science Collection</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Polymers</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhila, Natalia O.</au><au>Sapozhnikova, Kristina Yu</au><au>Kiselev, Evgeniy G.</au><au>Vasiliev, Alexander D.</au><au>Nemtsev, Ivan V.</au><au>Shishatskaya, Ekaterina I.</au><au>Volova, Tatiana G.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Properties of Degradable Polyhydroxyalkanoates (PHAs) Synthesized by a New Strain, Cupriavidus necator IBP/SFU-1, from Various Carbon Sources</atitle><jtitle>Polymers</jtitle><date>2021-09-17</date><risdate>2021</risdate><volume>13</volume><issue>18</issue><spage>3142</spage><pages>3142-</pages><issn>2073-4360</issn><eissn>2073-4360</eissn><abstract>The bacterial strain isolated from soil was identified as Cupriavidus necator IBP/SFU-1 and investigated as a PHA producer. The strain was found to be able to grow and synthesize PHAs under autotrophic conditions and showed a broad organotrophic potential towards different carbon sources: sugars, glycerol, fatty acids, and plant oils. The highest cell concentrations (7–8 g/L) and PHA contents were produced from oleic acid (78%), fructose, glucose, and palm oil (over 80%). The type of the carbon source influenced the PHA chemical composition and properties: when grown on oleic acid, the strain synthesized the P(3HB-co-3HV) copolymer; on plant oils, the P(3HB-co-3HV-co-3HHx) terpolymer, and on the other substrates, the P(3HB) homopolymer. The type of the carbon source influenced molecular-weight properties of PHAs: P(3HB) synthesized under autotrophic growth conditions, from CO2, had the highest number-average (290 ± 15 kDa) and weight-average (850 ± 25 kDa) molecular weights and the lowest polydispersity (2.9 ± 0.2); polymers synthesized from organic carbon sources showed increased polydispersity and reduced molecular weight. The carbon source was not found to affect the degree of crystallinity and thermal properties of the PHAs. The type of the carbon source determined not only PHA composition and molecular weight but also surface microstructure and porosity of the polymer films. The new strain can be recommended as a promising P(3HB) producer from palm oil, oleic acid, and sugars (fructose and glucose) and as a producer of P(3HB-co-3HV) from oleic acid and P(3HB-co-3HV-co-3HHx) from palm oil.</abstract><cop>Basel</cop><pub>MDPI AG</pub><pmid>34578042</pmid><doi>10.3390/polym13183142</doi><orcidid>https://orcid.org/0000-0001-6054-4200</orcidid><orcidid>https://orcid.org/0000-0001-8739-4062</orcidid><orcidid>https://orcid.org/0000-0003-4472-7087</orcidid><orcidid>https://orcid.org/0000-0002-4222-3099</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 2073-4360 |
| ispartof | Polymers, 2021-09, Vol.13 (18), p.3142 |
| issn | 2073-4360 2073-4360 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_8468435 |
| source | Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central Open Access; MDPI - Multidisciplinary Digital Publishing Institute; PubMed Central |
| subjects | Biomass Carbon Chemical composition Chromatography Copolymers Degree of crystallinity Fatty acids Fermentation Fructose Glucose Glycerol Lipids Mass spectrometry Microorganisms Microscopy Molecular weight Oleic acid Organic carbon Palm oil Phylogenetics Polydispersity Polyhydroxyalkanoates Polymer films Polymers Proteins Scientific imaging Software Soil investigations Soil porosity Substrates Sugar Terpolymers Thermodynamic properties Weight reduction |
| title | Properties of Degradable Polyhydroxyalkanoates (PHAs) Synthesized by a New Strain, Cupriavidus necator IBP/SFU-1, from Various Carbon Sources |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-25T13%3A37%3A18IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Properties%20of%20Degradable%20Polyhydroxyalkanoates%20(PHAs)%20Synthesized%20by%20a%20New%20Strain,%20Cupriavidus%20necator%20IBP/SFU-1,%20from%20Various%20Carbon%20Sources&rft.jtitle=Polymers&rft.au=Zhila,%20Natalia%20O.&rft.date=2021-09-17&rft.volume=13&rft.issue=18&rft.spage=3142&rft.pages=3142-&rft.issn=2073-4360&rft.eissn=2073-4360&rft_id=info:doi/10.3390/polym13183142&rft_dat=%3Cproquest_pubme%3E2576482637%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2576482637&rft_id=info:pmid/34578042&rfr_iscdi=true |