Methods and platforms for analysis of nucleic acids from single-cell based on microfluidics
Single-cell nucleic acid analysis aims at discovering the genetic differences between individual cells which is well known as the cellular heterogeneity. This technology facilitates cancer diagnosis, stem cell research, immune system analysis, and other life science applications. The conventional pl...
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| Veröffentlicht in: | Microfluidics and nanofluidics 2021-11, Vol.25 (11), p.87-87, Article 87 |
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| container_title | Microfluidics and nanofluidics |
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| creator | Liu, Luyao Dong, Xiaobin Tu, Yunping Miao, Guijun Zhang, Zhongping Zhang, Lulu Wei, Zewen Yu, Duli Qiu, Xianbo |
| description | Single-cell nucleic acid analysis aims at discovering the genetic differences between individual cells which is well known as the cellular heterogeneity. This technology facilitates cancer diagnosis, stem cell research, immune system analysis, and other life science applications. The conventional platforms for single-cell nucleic acid analysis more rely on manual operation or bulky devices. Recently, the emerging microfluidic technology has provided a perfect platform for single-cell nucleic acid analysis with the characteristic of accurate and automatic single-cell manipulation. In this review, we briefly summarized the procedure of single-cell nucleic acid analysis including single-cell isolation, single-cell lysis, nucleic acid amplification, and genetic analysis. And then, three representative microfluidic platforms for single-cell nucleic acid analysis are concluded as valve-, microwell-, and droplet-based platforms. Furthermore, we described the state-of-the-art integrated single-cell nucleic acid analysis systems based on the three platforms. Finally, the future development and challenges of microfluidics-based single-cell nucleic acid analysis are discussed as well. |
| doi_str_mv | 10.1007/s10404-021-02485-0 |
| format | Article |
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This technology facilitates cancer diagnosis, stem cell research, immune system analysis, and other life science applications. The conventional platforms for single-cell nucleic acid analysis more rely on manual operation or bulky devices. Recently, the emerging microfluidic technology has provided a perfect platform for single-cell nucleic acid analysis with the characteristic of accurate and automatic single-cell manipulation. In this review, we briefly summarized the procedure of single-cell nucleic acid analysis including single-cell isolation, single-cell lysis, nucleic acid amplification, and genetic analysis. And then, three representative microfluidic platforms for single-cell nucleic acid analysis are concluded as valve-, microwell-, and droplet-based platforms. Furthermore, we described the state-of-the-art integrated single-cell nucleic acid analysis systems based on the three platforms. Finally, the future development and challenges of microfluidics-based single-cell nucleic acid analysis are discussed as well.</description><identifier>ISSN: 1613-4982</identifier><identifier>EISSN: 1613-4990</identifier><identifier>DOI: 10.1007/s10404-021-02485-0</identifier><identifier>PMID: 34580578</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Acids ; Analytical Chemistry ; Biomedical Engineering and Bioengineering ; Engineering ; Engineering Fluid Dynamics ; Genetic analysis ; Heterogeneity ; Immune system ; Immunity ; Lysis ; Microfluidics ; Nanotechnology and Microengineering ; Nucleic acids ; Platforms ; Review ; Stem cells ; Systems analysis ; Technology ; Work platforms</subject><ispartof>Microfluidics and nanofluidics, 2021-11, Vol.25 (11), p.87-87, Article 87</ispartof><rights>The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2021</rights><rights>The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2021.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c474t-43e7d103cb47f118c6d04afc0934278756ac00cadc11ec9d4187aab17354ad2d3</citedby><cites>FETCH-LOGICAL-c474t-43e7d103cb47f118c6d04afc0934278756ac00cadc11ec9d4187aab17354ad2d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10404-021-02485-0$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10404-021-02485-0$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>230,314,780,784,885,27923,27924,41487,42556,51318</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34580578$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Liu, Luyao</creatorcontrib><creatorcontrib>Dong, Xiaobin</creatorcontrib><creatorcontrib>Tu, Yunping</creatorcontrib><creatorcontrib>Miao, Guijun</creatorcontrib><creatorcontrib>Zhang, Zhongping</creatorcontrib><creatorcontrib>Zhang, Lulu</creatorcontrib><creatorcontrib>Wei, Zewen</creatorcontrib><creatorcontrib>Yu, Duli</creatorcontrib><creatorcontrib>Qiu, Xianbo</creatorcontrib><title>Methods and platforms for analysis of nucleic acids from single-cell based on microfluidics</title><title>Microfluidics and nanofluidics</title><addtitle>Microfluid Nanofluid</addtitle><addtitle>Microfluid Nanofluidics</addtitle><description>Single-cell nucleic acid analysis aims at discovering the genetic differences between individual cells which is well known as the cellular heterogeneity. This technology facilitates cancer diagnosis, stem cell research, immune system analysis, and other life science applications. The conventional platforms for single-cell nucleic acid analysis more rely on manual operation or bulky devices. Recently, the emerging microfluidic technology has provided a perfect platform for single-cell nucleic acid analysis with the characteristic of accurate and automatic single-cell manipulation. In this review, we briefly summarized the procedure of single-cell nucleic acid analysis including single-cell isolation, single-cell lysis, nucleic acid amplification, and genetic analysis. And then, three representative microfluidic platforms for single-cell nucleic acid analysis are concluded as valve-, microwell-, and droplet-based platforms. Furthermore, we described the state-of-the-art integrated single-cell nucleic acid analysis systems based on the three platforms. Finally, the future development and challenges of microfluidics-based single-cell nucleic acid analysis are discussed as well.</description><subject>Acids</subject><subject>Analytical Chemistry</subject><subject>Biomedical Engineering and Bioengineering</subject><subject>Engineering</subject><subject>Engineering Fluid Dynamics</subject><subject>Genetic analysis</subject><subject>Heterogeneity</subject><subject>Immune system</subject><subject>Immunity</subject><subject>Lysis</subject><subject>Microfluidics</subject><subject>Nanotechnology and Microengineering</subject><subject>Nucleic acids</subject><subject>Platforms</subject><subject>Review</subject><subject>Stem cells</subject><subject>Systems analysis</subject><subject>Technology</subject><subject>Work 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from single-cell based on microfluidics</title><author>Liu, Luyao ; Dong, Xiaobin ; Tu, Yunping ; Miao, Guijun ; Zhang, Zhongping ; Zhang, Lulu ; Wei, Zewen ; Yu, Duli ; Qiu, Xianbo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c474t-43e7d103cb47f118c6d04afc0934278756ac00cadc11ec9d4187aab17354ad2d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Acids</topic><topic>Analytical Chemistry</topic><topic>Biomedical Engineering and Bioengineering</topic><topic>Engineering</topic><topic>Engineering Fluid Dynamics</topic><topic>Genetic analysis</topic><topic>Heterogeneity</topic><topic>Immune system</topic><topic>Immunity</topic><topic>Lysis</topic><topic>Microfluidics</topic><topic>Nanotechnology and Microengineering</topic><topic>Nucleic acids</topic><topic>Platforms</topic><topic>Review</topic><topic>Stem cells</topic><topic>Systems 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Nanofluidics</addtitle><date>2021-11-01</date><risdate>2021</risdate><volume>25</volume><issue>11</issue><spage>87</spage><epage>87</epage><pages>87-87</pages><artnum>87</artnum><issn>1613-4982</issn><eissn>1613-4990</eissn><abstract>Single-cell nucleic acid analysis aims at discovering the genetic differences between individual cells which is well known as the cellular heterogeneity. This technology facilitates cancer diagnosis, stem cell research, immune system analysis, and other life science applications. The conventional platforms for single-cell nucleic acid analysis more rely on manual operation or bulky devices. Recently, the emerging microfluidic technology has provided a perfect platform for single-cell nucleic acid analysis with the characteristic of accurate and automatic single-cell manipulation. In this review, we briefly summarized the procedure of single-cell nucleic acid analysis including single-cell isolation, single-cell lysis, nucleic acid amplification, and genetic analysis. And then, three representative microfluidic platforms for single-cell nucleic acid analysis are concluded as valve-, microwell-, and droplet-based platforms. Furthermore, we described the state-of-the-art integrated single-cell nucleic acid analysis systems based on the three platforms. Finally, the future development and challenges of microfluidics-based single-cell nucleic acid analysis are discussed as well.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>34580578</pmid><doi>10.1007/s10404-021-02485-0</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Microfluidics and nanofluidics, 2021-11, Vol.25 (11), p.87-87, Article 87 |
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| subjects | Acids Analytical Chemistry Biomedical Engineering and Bioengineering Engineering Engineering Fluid Dynamics Genetic analysis Heterogeneity Immune system Immunity Lysis Microfluidics Nanotechnology and Microengineering Nucleic acids Platforms Review Stem cells Systems analysis Technology Work platforms |
| title | Methods and platforms for analysis of nucleic acids from single-cell based on microfluidics |
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