Prebiotic Enriched Exclusive Enteral Nutrition Suppresses Colitis via Gut Microbiome Modulation and Expansion of Anti-inflammatory T Cells in a Mouse Model of Colitis
Exclusive enteral nutrition (EEN) is used to treat pediatric Crohn’s disease (CD), but therapeutic benefits are variable, and EEN can lead to microbial dysbiosis. Because of reported lower efficacy EEN is not routinely used to treat pediatric ulcerative colitis (UC). Inulin-type fructans (IN) benefi...
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Veröffentlicht in: | Cellular and molecular gastroenterology and hepatology 2021-01, Vol.12 (4), p.1251-1266 |
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creator | Lunken, Genelle R. Tsai, Kevin Schick, Alana Lisko, Daniel J. Cook, Laura Vallance, Bruce A. Jacobson, Kevan |
description | Exclusive enteral nutrition (EEN) is used to treat pediatric Crohn’s disease (CD), but therapeutic benefits are variable, and EEN can lead to microbial dysbiosis. Because of reported lower efficacy EEN is not routinely used to treat pediatric ulcerative colitis (UC). Inulin-type fructans (IN) beneficially modulate the gut microbiome and promote expansion of anti-inflammatory immune cells. We hypothesized that enriching EEN with IN (EEN IN) would enhance treatment efficacy. To test this, we examined the effects of EEN IN on colitis development, the gut microbiome, and CD4+ T cells using an adoptive T-cell transfer model of colitis.
TCR-β deficient (-/-) mice were randomized to 1 of 4 groups: (1) Control, (2) Chow, (3) EEN, and (4) EEN IN, and naive CD4+ T cells were adoptively transferred into groups 2–4, after which mice were monitored for 5 weeks before experimental endpoint.
Mice fed EEN IN showed greater colitis protection, with colonic shortening, goblet cell, and crypt density loss reduced compared with EEN fed mice and reduced disease activity and immune cell infiltration compared with chow fed mice, and less crypt hyperplasia and higher survival compared with both groups. EEN IN mice had less deterioration in the colonic mucus layer and had increased levels of Foxp3+IL-10+ and Rorγt+IL-22+ and reduced levels of Tbet+IFNγ+ and Tbet+TNF+ CD4+ T cells. EEN IN also led to higher butyrate concentrations, Bifidobacterium spp. and Anaerostipes caccae relative abundance, and lower [Clostridium] innocuum group spp. and Escherichia-Shigella spp. relative abundance.
The EEN IN group showed reduced colitis development as compared with the chow and EEN groups. This highlights the potential benefits of EEN IN as a novel induction therapy for pediatric CD and UC patients.
[Display omitted] |
doi_str_mv | 10.1016/j.jcmgh.2021.06.011 |
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TCR-β deficient (-/-) mice were randomized to 1 of 4 groups: (1) Control, (2) Chow, (3) EEN, and (4) EEN IN, and naive CD4+ T cells were adoptively transferred into groups 2–4, after which mice were monitored for 5 weeks before experimental endpoint.
Mice fed EEN IN showed greater colitis protection, with colonic shortening, goblet cell, and crypt density loss reduced compared with EEN fed mice and reduced disease activity and immune cell infiltration compared with chow fed mice, and less crypt hyperplasia and higher survival compared with both groups. EEN IN mice had less deterioration in the colonic mucus layer and had increased levels of Foxp3+IL-10+ and Rorγt+IL-22+ and reduced levels of Tbet+IFNγ+ and Tbet+TNF+ CD4+ T cells. EEN IN also led to higher butyrate concentrations, Bifidobacterium spp. and Anaerostipes caccae relative abundance, and lower [Clostridium] innocuum group spp. and Escherichia-Shigella spp. relative abundance.
The EEN IN group showed reduced colitis development as compared with the chow and EEN groups. This highlights the potential benefits of EEN IN as a novel induction therapy for pediatric CD and UC patients.
[Display omitted]</description><identifier>ISSN: 2352-345X</identifier><identifier>EISSN: 2352-345X</identifier><identifier>DOI: 10.1016/j.jcmgh.2021.06.011</identifier><identifier>PMID: 34214707</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; Biomarkers ; Colitis - etiology ; Colitis - metabolism ; Colitis - pathology ; Colitis - therapy ; Disease Models, Animal ; Disease Susceptibility ; Enteral Nutrition - methods ; Forkhead Transcription Factors - metabolism ; Gastrointestinal Microbiome ; Immunomodulation ; Inflammatory Bowel Disease ; Mice ; Mice, Knockout ; Microbiome ; Original Research ; Phenotype ; Prebiotic ; Prebiotics ; Regulatory T Cells ; T-Lymphocytes - immunology ; T-Lymphocytes - metabolism</subject><ispartof>Cellular and molecular gastroenterology and hepatology, 2021-01, Vol.12 (4), p.1251-1266</ispartof><rights>2021 The Authors</rights><rights>Copyright © 2021 The Authors. Published by Elsevier Inc. All rights reserved.</rights><rights>2021 The Authors 2021</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c459t-5a27b0bcae5dfd85e911f4ba14c08b26d938b6c2bc339d5d4203a18ac29dc8033</citedby><cites>FETCH-LOGICAL-c459t-5a27b0bcae5dfd85e911f4ba14c08b26d938b6c2bc339d5d4203a18ac29dc8033</cites><orcidid>0000-0001-7269-8557</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8453203/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8453203/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34214707$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lunken, Genelle R.</creatorcontrib><creatorcontrib>Tsai, Kevin</creatorcontrib><creatorcontrib>Schick, Alana</creatorcontrib><creatorcontrib>Lisko, Daniel J.</creatorcontrib><creatorcontrib>Cook, Laura</creatorcontrib><creatorcontrib>Vallance, Bruce A.</creatorcontrib><creatorcontrib>Jacobson, Kevan</creatorcontrib><title>Prebiotic Enriched Exclusive Enteral Nutrition Suppresses Colitis via Gut Microbiome Modulation and Expansion of Anti-inflammatory T Cells in a Mouse Model of Colitis</title><title>Cellular and molecular gastroenterology and hepatology</title><addtitle>Cell Mol Gastroenterol Hepatol</addtitle><description>Exclusive enteral nutrition (EEN) is used to treat pediatric Crohn’s disease (CD), but therapeutic benefits are variable, and EEN can lead to microbial dysbiosis. Because of reported lower efficacy EEN is not routinely used to treat pediatric ulcerative colitis (UC). Inulin-type fructans (IN) beneficially modulate the gut microbiome and promote expansion of anti-inflammatory immune cells. We hypothesized that enriching EEN with IN (EEN IN) would enhance treatment efficacy. To test this, we examined the effects of EEN IN on colitis development, the gut microbiome, and CD4+ T cells using an adoptive T-cell transfer model of colitis.
TCR-β deficient (-/-) mice were randomized to 1 of 4 groups: (1) Control, (2) Chow, (3) EEN, and (4) EEN IN, and naive CD4+ T cells were adoptively transferred into groups 2–4, after which mice were monitored for 5 weeks before experimental endpoint.
Mice fed EEN IN showed greater colitis protection, with colonic shortening, goblet cell, and crypt density loss reduced compared with EEN fed mice and reduced disease activity and immune cell infiltration compared with chow fed mice, and less crypt hyperplasia and higher survival compared with both groups. EEN IN mice had less deterioration in the colonic mucus layer and had increased levels of Foxp3+IL-10+ and Rorγt+IL-22+ and reduced levels of Tbet+IFNγ+ and Tbet+TNF+ CD4+ T cells. EEN IN also led to higher butyrate concentrations, Bifidobacterium spp. and Anaerostipes caccae relative abundance, and lower [Clostridium] innocuum group spp. and Escherichia-Shigella spp. relative abundance.
The EEN IN group showed reduced colitis development as compared with the chow and EEN groups. This highlights the potential benefits of EEN IN as a novel induction therapy for pediatric CD and UC patients.
[Display omitted]</description><subject>Animals</subject><subject>Biomarkers</subject><subject>Colitis - etiology</subject><subject>Colitis - metabolism</subject><subject>Colitis - pathology</subject><subject>Colitis - therapy</subject><subject>Disease Models, Animal</subject><subject>Disease Susceptibility</subject><subject>Enteral Nutrition - methods</subject><subject>Forkhead Transcription Factors - metabolism</subject><subject>Gastrointestinal Microbiome</subject><subject>Immunomodulation</subject><subject>Inflammatory Bowel Disease</subject><subject>Mice</subject><subject>Mice, Knockout</subject><subject>Microbiome</subject><subject>Original Research</subject><subject>Phenotype</subject><subject>Prebiotic</subject><subject>Prebiotics</subject><subject>Regulatory T Cells</subject><subject>T-Lymphocytes - immunology</subject><subject>T-Lymphocytes - metabolism</subject><issn>2352-345X</issn><issn>2352-345X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9Uctu1DAUjRCIVqVfgIS8ZJPUjziPBUjVaFoqtYBEkdhZjn3T8Sixg-2M6A_xnTgzQ1U2rOx7fR6-92TZW4ILgkl1sS22anzYFBRTUuCqwIS8yE4p4zRnJf_x8tn9JDsPYYsxJmVd1Zi_zk5YSVOB69Ps91cPnXHRKLS23qgNaLT-pYY5mB2kVgQvB_R5jt5E4yz6Nk-ThxAgoJUbUi-gnZHoeo7ozijvktYI6M7peZB7grSL4CRtWCrXo0sbTW5sP8hxlNH5R3SPVjAMAZmETtQ57AVgWNBHkzfZq14OAc6P51n2_Wp9v_qU3365vlld3uaq5G3MuaR1hzslgeteNxxaQvqyk6RUuOlopVvWdJWinWKs1VyXFDNJGqloq1WDGTvLPh50p7kbQSuwMc0vJm9G6R-Fk0b8-2LNRjy4nWhKzuhe4P1RwLufM4QoRhNUGk9aSJMJysuGtW1d8wRlB2haWwge-icbgsUSstiKfchiCVngSqSQE-vd8x8-cf5GmgAfDgBIe9oZ8CIoA1aBNh5UFNqZ_xr8AWwOveg</recordid><startdate>20210101</startdate><enddate>20210101</enddate><creator>Lunken, Genelle R.</creator><creator>Tsai, Kevin</creator><creator>Schick, Alana</creator><creator>Lisko, Daniel J.</creator><creator>Cook, Laura</creator><creator>Vallance, Bruce A.</creator><creator>Jacobson, Kevan</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0001-7269-8557</orcidid></search><sort><creationdate>20210101</creationdate><title>Prebiotic Enriched Exclusive Enteral Nutrition Suppresses Colitis via Gut Microbiome Modulation and Expansion of Anti-inflammatory T Cells in a Mouse Model of Colitis</title><author>Lunken, Genelle R. ; 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Because of reported lower efficacy EEN is not routinely used to treat pediatric ulcerative colitis (UC). Inulin-type fructans (IN) beneficially modulate the gut microbiome and promote expansion of anti-inflammatory immune cells. We hypothesized that enriching EEN with IN (EEN IN) would enhance treatment efficacy. To test this, we examined the effects of EEN IN on colitis development, the gut microbiome, and CD4+ T cells using an adoptive T-cell transfer model of colitis.
TCR-β deficient (-/-) mice were randomized to 1 of 4 groups: (1) Control, (2) Chow, (3) EEN, and (4) EEN IN, and naive CD4+ T cells were adoptively transferred into groups 2–4, after which mice were monitored for 5 weeks before experimental endpoint.
Mice fed EEN IN showed greater colitis protection, with colonic shortening, goblet cell, and crypt density loss reduced compared with EEN fed mice and reduced disease activity and immune cell infiltration compared with chow fed mice, and less crypt hyperplasia and higher survival compared with both groups. EEN IN mice had less deterioration in the colonic mucus layer and had increased levels of Foxp3+IL-10+ and Rorγt+IL-22+ and reduced levels of Tbet+IFNγ+ and Tbet+TNF+ CD4+ T cells. EEN IN also led to higher butyrate concentrations, Bifidobacterium spp. and Anaerostipes caccae relative abundance, and lower [Clostridium] innocuum group spp. and Escherichia-Shigella spp. relative abundance.
The EEN IN group showed reduced colitis development as compared with the chow and EEN groups. This highlights the potential benefits of EEN IN as a novel induction therapy for pediatric CD and UC patients.
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subjects | Animals Biomarkers Colitis - etiology Colitis - metabolism Colitis - pathology Colitis - therapy Disease Models, Animal Disease Susceptibility Enteral Nutrition - methods Forkhead Transcription Factors - metabolism Gastrointestinal Microbiome Immunomodulation Inflammatory Bowel Disease Mice Mice, Knockout Microbiome Original Research Phenotype Prebiotic Prebiotics Regulatory T Cells T-Lymphocytes - immunology T-Lymphocytes - metabolism |
title | Prebiotic Enriched Exclusive Enteral Nutrition Suppresses Colitis via Gut Microbiome Modulation and Expansion of Anti-inflammatory T Cells in a Mouse Model of Colitis |
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