High‐Resolution Imaging of Human Viruses in Liquid Droplets
Liquid‐phase electron microscopy (LP‐EM) is an exciting new area in the materials imaging field, providing unprecedented views of molecular processes. Time‐resolved insights from LP‐EM studies are a strong complement to the remarkable results achievable with other high‐resolution techniques. Here, t...
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| Veröffentlicht in: | Advanced materials (Weinheim) 2021-09, Vol.33 (37), p.e2103221-n/a |
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| creator | Jonaid, GM Dearnaley, William J. Casasanta, Michael A. Kaylor, Liam Berry, Samantha Dukes, Madeline J. Spilman, Michael S. Gray, Jennifer L. Kelly, Deborah F. |
| description | Liquid‐phase electron microscopy (LP‐EM) is an exciting new area in the materials imaging field, providing unprecedented views of molecular processes. Time‐resolved insights from LP‐EM studies are a strong complement to the remarkable results achievable with other high‐resolution techniques. Here, the opportunities to expand LP‐EM technology beyond 2D temporal assessments and into the 3D regime are described. The results show new structures and dynamic insights of human viruses contained in minute volumes of liquid while acquired in a rapid timeframe. To develop this strategy, adeno‐associated virus (AAV) is used as a model system. AAV is a well‐known gene therapy vehicle with current applications involving drug delivery and vaccine development for COVID‐19. Improving the understanding of the physical properties of biological entities in a liquid state, as maintained in the human body, has broad societal implications for human health and disease.
The first high‐resolution view of biological assemblies contained in a liquid environment is presented. The foundation for analyzing structure and dynamics of soft materials in real‐time and in 3D is established. |
| doi_str_mv | 10.1002/adma.202103221 |
| format | Article |
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The first high‐resolution view of biological assemblies contained in a liquid environment is presented. The foundation for analyzing structure and dynamics of soft materials in real‐time and in 3D is established.</description><subject>adeno‐associated virus</subject><subject>Biological properties</subject><subject>COVID-19</subject><subject>COVID-19 Vaccines</subject><subject>Cryoelectron Microscopy - methods</subject><subject>Dependovirus</subject><subject>direct electron detector</subject><subject>Drug Delivery Systems</subject><subject>Equipment Design</subject><subject>Gene therapy</subject><subject>Genetic Therapy</subject><subject>HEK293 Cells - virology</subject><subject>Humans</subject><subject>Hydrogen-Ion Concentration</subject><subject>Immunoglobulin G - chemistry</subject><subject>liquid‐phase electron microscopy</subject><subject>Materials science</subject><subject>Materials Testing</subject><subject>Particle Size</subject><subject>Physical properties</subject><subject>SARS-CoV-2</subject><subject>silicon nitride</subject><subject>Technology assessment</subject><subject>Viruses</subject><issn>0935-9648</issn><issn>1521-4095</issn><issn>1521-4095</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkcFOGzEQhi3UClLKlSNaqRcum854be_6AFIELUEKqlS1vVrOZhyMdtfBzrbi1kfoM_ZJ2Cg0hV56msN882l-_YwdI4wRgL-3i9aOOXCEgnPcYyOUHHMBWr5iI9CFzLUS1QF7k9IdAGgFap8dFKIALgBH7Gzql7e_f_76TCk0_dqHLrtu7dJ3yyy4bNq3tsu--dgnSpnvspm_7_0iu4xh1dA6vWWvnW0SHT3NQ_b144cvF9N89unq-mIyy2uhKsxpjqhrp-paICnnuFbKkZQkhbXIHfASqJKiFFQ6MadqbnW9UEoWJXIUvDhk51vvqp-3tKipW0fbmFX0rY0PJlhvXm46f2uW4buphACh9SA4fRLEcN9TWpvWp5qaxnYU-mS4lBKxKBQM6Lt_0LvQx26IN1Al54BQbajxlqpjSCmS2z2DYDbNmE0zZtfMcHDyPMIO_1PFAOgt8MM39PAfnZlc3kz-yh8B5amaag</recordid><startdate>20210901</startdate><enddate>20210901</enddate><creator>Jonaid, GM</creator><creator>Dearnaley, William J.</creator><creator>Casasanta, Michael A.</creator><creator>Kaylor, Liam</creator><creator>Berry, Samantha</creator><creator>Dukes, Madeline J.</creator><creator>Spilman, Michael S.</creator><creator>Gray, Jennifer L.</creator><creator>Kelly, Deborah F.</creator><general>Wiley Subscription Services, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SR</scope><scope>8BQ</scope><scope>8FD</scope><scope>JG9</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-7341-7435</orcidid></search><sort><creationdate>20210901</creationdate><title>High‐Resolution Imaging of Human Viruses in Liquid Droplets</title><author>Jonaid, GM ; Dearnaley, William J. ; Casasanta, Michael A. ; Kaylor, Liam ; Berry, Samantha ; Dukes, Madeline J. ; Spilman, Michael S. ; Gray, Jennifer L. ; Kelly, Deborah F.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4681-eb119cf6cc41e6ff2966fe55e54aa12f0270e85474e7f4be8ba9cd66537121423</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>adeno‐associated virus</topic><topic>Biological properties</topic><topic>COVID-19</topic><topic>COVID-19 Vaccines</topic><topic>Cryoelectron Microscopy - methods</topic><topic>Dependovirus</topic><topic>direct electron detector</topic><topic>Drug Delivery Systems</topic><topic>Equipment Design</topic><topic>Gene therapy</topic><topic>Genetic Therapy</topic><topic>HEK293 Cells - virology</topic><topic>Humans</topic><topic>Hydrogen-Ion Concentration</topic><topic>Immunoglobulin G - chemistry</topic><topic>liquid‐phase electron microscopy</topic><topic>Materials science</topic><topic>Materials Testing</topic><topic>Particle Size</topic><topic>Physical properties</topic><topic>SARS-CoV-2</topic><topic>silicon nitride</topic><topic>Technology assessment</topic><topic>Viruses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Jonaid, GM</creatorcontrib><creatorcontrib>Dearnaley, William J.</creatorcontrib><creatorcontrib>Casasanta, Michael A.</creatorcontrib><creatorcontrib>Kaylor, Liam</creatorcontrib><creatorcontrib>Berry, Samantha</creatorcontrib><creatorcontrib>Dukes, Madeline J.</creatorcontrib><creatorcontrib>Spilman, Michael S.</creatorcontrib><creatorcontrib>Gray, Jennifer L.</creatorcontrib><creatorcontrib>Kelly, Deborah F.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Engineered Materials Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Materials Research Database</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Advanced materials (Weinheim)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jonaid, GM</au><au>Dearnaley, William J.</au><au>Casasanta, Michael A.</au><au>Kaylor, Liam</au><au>Berry, Samantha</au><au>Dukes, Madeline J.</au><au>Spilman, Michael S.</au><au>Gray, Jennifer L.</au><au>Kelly, Deborah F.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>High‐Resolution Imaging of Human Viruses in Liquid Droplets</atitle><jtitle>Advanced materials (Weinheim)</jtitle><addtitle>Adv Mater</addtitle><date>2021-09-01</date><risdate>2021</risdate><volume>33</volume><issue>37</issue><spage>e2103221</spage><epage>n/a</epage><pages>e2103221-n/a</pages><issn>0935-9648</issn><issn>1521-4095</issn><eissn>1521-4095</eissn><abstract>Liquid‐phase electron microscopy (LP‐EM) is an exciting new area in the materials imaging field, providing unprecedented views of molecular processes. 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| subjects | adeno‐associated virus Biological properties COVID-19 COVID-19 Vaccines Cryoelectron Microscopy - methods Dependovirus direct electron detector Drug Delivery Systems Equipment Design Gene therapy Genetic Therapy HEK293 Cells - virology Humans Hydrogen-Ion Concentration Immunoglobulin G - chemistry liquid‐phase electron microscopy Materials science Materials Testing Particle Size Physical properties SARS-CoV-2 silicon nitride Technology assessment Viruses |
| title | High‐Resolution Imaging of Human Viruses in Liquid Droplets |
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