Femtomolar SARS-CoV-2 Antigen Detection Using the Microbubbling Digital Assay with Smartphone Readout Enables Antigen Burden Quantitation and Tracking

Abstract Background High-sensitivity severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) antigen assays are desirable to mitigate false negative results. Limited data are available to quantify and track SARS-CoV-2 antigen burden in respiratory samples from different populations. Methods We...

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Veröffentlicht in:	Clinical chemistry (Baltimore, Md.) Md.), 2022-01, Vol.68 (1), p.230-239
Hauptverfasser:	Chen, Hui, Li, Zhao, Feng, Sheng, Richard-Greenblatt, Melissa, Hutson, Emily, Andrianus, Stefen, Glaser, Laurel J, Rodino, Kyle G, Qian, Jianing, Jayaraman, Dinesh, Collman, Ronald G, Glascock, Abigail, Bushman, Frederic D, Lee, Jae Seung, Cherry, Sara, Fausto, Alejandra, Weiss, Susan R, Koo, Hyun, Corby, Patricia M, Oceguera, Alfonso, O’Doherty, Una, Garfall, Alfred L, Vogl, Dan T, Stadtmauer, Edward A, Wang, Ping
Format:	Artikel
Sprache:	eng
Schlagworte:	Antigens Antigens, Viral - analysis Assaying Blood cancer Computer viruses Computer vision Coronaviruses COVID-19 COVID-19 - diagnosis COVID-19 Testing - methods Humans Immunocompromised hosts Learning algorithms Longitudinal studies Machine Learning Methods Microbiological assay Nucleic acids Nucleocapsids Patients Polymerase chain reaction Quantitation Respiratory diseases Reverse transcription SARS-CoV-2 Sensitivity and Specificity Severe acute respiratory syndrome coronavirus 2 Smartphone Smartphones Tracking Viral diseases
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