Multiple roles for PARP1 in ALC1-dependent nucleosome remodeling
The SNF2 family ATPase Amplified in Liver Cancer 1 (ALC1) is the only chromatin remodeling enzyme with a poly(ADP-ribose) (PAR) binding macrodomain. ALC1 functions together with poly(ADP-ribose) polymerase PARP1 to remodel nucleosomes. Activation of ALC1 cryptic ATPase activity and the subsequent nu...
Gespeichert in:
| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 2021-09, Vol.118 (36), p.1-7 |
|---|---|
| Hauptverfasser: | , , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 7 |
|---|---|
| container_issue | 36 |
| container_start_page | 1 |
| container_title | Proceedings of the National Academy of Sciences - PNAS |
| container_volume | 118 |
| creator | Ooi, Soon-Keat Sato, Shigeo Tomomori-Sato, Chieri Zhang, Ying Wen, Zhihui Banks, Charles A. S. Washburn, Michael P. Unruh, Jay R. Florens, Laurence Conaway, Ronald C. Conaway, Joan W. |
| description | The SNF2 family ATPase Amplified in Liver Cancer 1 (ALC1) is the only chromatin remodeling enzyme with a poly(ADP-ribose) (PAR) binding macrodomain. ALC1 functions together with poly(ADP-ribose) polymerase PARP1 to remodel nucleosomes. Activation of ALC1 cryptic ATPase activity and the subsequent nucleosome remodeling requires binding of its macrodomain to PAR chains synthesized by PARP1 and NAD⁺. A key question is whether PARP1 has a role(s) in ALC1-dependent nucleosome remodeling beyond simply synthesizing the PAR chains needed to activate the ALC1 ATPase. Here, we identify PARP1 separation-of-function mutants that activate ALC1 ATPase but do not support nucleosome remodeling by ALC1. Investigation of these mutants has revealed multiple functions for PARP1 in ALC1-dependent nucleosome remodeling and provides insights into its multifaceted role in chromatin remodeling. |
| doi_str_mv | 10.1073/pnas.2107277118 |
| format | Article |
| fullrecord | <record><control><sourceid>jstor_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_8433585</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><jstor_id>27075416</jstor_id><sourcerecordid>27075416</sourcerecordid><originalsourceid>FETCH-LOGICAL-c415t-5a0b4daebc8eec30ec036f443e9d790a6939a4399cac68e5d5dd86beb55f7053</originalsourceid><addsrcrecordid>eNpVkM1v1DAQxS0EokvhzAmUI5e04_j7glit-JK2aoV6txx7UlI5cbATJP77ZrVloacZ6f3mzdMj5C2FCwqKXU6jKxfNujZKUaqfkQ0FQ2vJDTwnG4BG1Zo3_Iy8KuUeAIzQ8JKcMc6lkI3YkE9XS5z7KWKVU8RSdSlXN9sfN7Tqx2q739E64IRjwHGuxsVHTCUNK4xDChj78e41edG5WPDN4zwnt18-3-6-1fvrr993233tORVzLRy0PDhsvUb0DNADkx3nDE1QBpw0zDjOjPHOS40iiBC0bLEVolMg2Dn5eLSdlnbA4Nc82UU75X5w-Y9NrrdPlbH_ae_Sb6s5Y0IfDD48GuT0a8Ey26EvHmN0I6al2EZI3QghFV_RyyPqcyolY3d6Q8EeareH2u2_2teL9_-nO_F_e16Bd0fgvswpn_RGgRKcSvYAptOI8w</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2568255674</pqid></control><display><type>article</type><title>Multiple roles for PARP1 in ALC1-dependent nucleosome remodeling</title><source>Jstor Complete Legacy</source><source>MEDLINE</source><source>PubMed Central</source><source>Alma/SFX Local Collection</source><source>Free Full-Text Journals in Chemistry</source><creator>Ooi, Soon-Keat ; Sato, Shigeo ; Tomomori-Sato, Chieri ; Zhang, Ying ; Wen, Zhihui ; Banks, Charles A. S. ; Washburn, Michael P. ; Unruh, Jay R. ; Florens, Laurence ; Conaway, Ronald C. ; Conaway, Joan W.</creator><creatorcontrib>Ooi, Soon-Keat ; Sato, Shigeo ; Tomomori-Sato, Chieri ; Zhang, Ying ; Wen, Zhihui ; Banks, Charles A. S. ; Washburn, Michael P. ; Unruh, Jay R. ; Florens, Laurence ; Conaway, Ronald C. ; Conaway, Joan W.</creatorcontrib><description>The SNF2 family ATPase Amplified in Liver Cancer 1 (ALC1) is the only chromatin remodeling enzyme with a poly(ADP-ribose) (PAR) binding macrodomain. ALC1 functions together with poly(ADP-ribose) polymerase PARP1 to remodel nucleosomes. Activation of ALC1 cryptic ATPase activity and the subsequent nucleosome remodeling requires binding of its macrodomain to PAR chains synthesized by PARP1 and NAD⁺. A key question is whether PARP1 has a role(s) in ALC1-dependent nucleosome remodeling beyond simply synthesizing the PAR chains needed to activate the ALC1 ATPase. Here, we identify PARP1 separation-of-function mutants that activate ALC1 ATPase but do not support nucleosome remodeling by ALC1. Investigation of these mutants has revealed multiple functions for PARP1 in ALC1-dependent nucleosome remodeling and provides insights into its multifaceted role in chromatin remodeling.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.2107277118</identifier><identifier>PMID: 34465625</identifier><language>eng</language><publisher>United States: National Academy of Sciences</publisher><subject>Biological Sciences ; Cell Line, Tumor ; Chromatin Assembly and Disassembly ; DNA Helicases - metabolism ; DNA Repair ; DNA-Binding Proteins - metabolism ; Humans ; Nucleosomes - metabolism ; Poly (ADP-Ribose) Polymerase-1 - metabolism</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 2021-09, Vol.118 (36), p.1-7</ispartof><rights>2021</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c415t-5a0b4daebc8eec30ec036f443e9d790a6939a4399cac68e5d5dd86beb55f7053</citedby><cites>FETCH-LOGICAL-c415t-5a0b4daebc8eec30ec036f443e9d790a6939a4399cac68e5d5dd86beb55f7053</cites><orcidid>0000-0001-7568-2585 ; 0000-0003-3077-4990 ; 0000-0002-9310-6650 ; 0000-0002-2786-0663 ; 0000-0002-0404-2940</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/27075416$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/27075416$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,723,776,780,799,881,27901,27902,53766,53768,57992,58225</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34465625$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ooi, Soon-Keat</creatorcontrib><creatorcontrib>Sato, Shigeo</creatorcontrib><creatorcontrib>Tomomori-Sato, Chieri</creatorcontrib><creatorcontrib>Zhang, Ying</creatorcontrib><creatorcontrib>Wen, Zhihui</creatorcontrib><creatorcontrib>Banks, Charles A. S.</creatorcontrib><creatorcontrib>Washburn, Michael P.</creatorcontrib><creatorcontrib>Unruh, Jay R.</creatorcontrib><creatorcontrib>Florens, Laurence</creatorcontrib><creatorcontrib>Conaway, Ronald C.</creatorcontrib><creatorcontrib>Conaway, Joan W.</creatorcontrib><title>Multiple roles for PARP1 in ALC1-dependent nucleosome remodeling</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>The SNF2 family ATPase Amplified in Liver Cancer 1 (ALC1) is the only chromatin remodeling enzyme with a poly(ADP-ribose) (PAR) binding macrodomain. ALC1 functions together with poly(ADP-ribose) polymerase PARP1 to remodel nucleosomes. Activation of ALC1 cryptic ATPase activity and the subsequent nucleosome remodeling requires binding of its macrodomain to PAR chains synthesized by PARP1 and NAD⁺. A key question is whether PARP1 has a role(s) in ALC1-dependent nucleosome remodeling beyond simply synthesizing the PAR chains needed to activate the ALC1 ATPase. Here, we identify PARP1 separation-of-function mutants that activate ALC1 ATPase but do not support nucleosome remodeling by ALC1. Investigation of these mutants has revealed multiple functions for PARP1 in ALC1-dependent nucleosome remodeling and provides insights into its multifaceted role in chromatin remodeling.</description><subject>Biological Sciences</subject><subject>Cell Line, Tumor</subject><subject>Chromatin Assembly and Disassembly</subject><subject>DNA Helicases - metabolism</subject><subject>DNA Repair</subject><subject>DNA-Binding Proteins - metabolism</subject><subject>Humans</subject><subject>Nucleosomes - metabolism</subject><subject>Poly (ADP-Ribose) Polymerase-1 - metabolism</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkM1v1DAQxS0EokvhzAmUI5e04_j7glit-JK2aoV6txx7UlI5cbATJP77ZrVloacZ6f3mzdMj5C2FCwqKXU6jKxfNujZKUaqfkQ0FQ2vJDTwnG4BG1Zo3_Iy8KuUeAIzQ8JKcMc6lkI3YkE9XS5z7KWKVU8RSdSlXN9sfN7Tqx2q739E64IRjwHGuxsVHTCUNK4xDChj78e41edG5WPDN4zwnt18-3-6-1fvrr993233tORVzLRy0PDhsvUb0DNADkx3nDE1QBpw0zDjOjPHOS40iiBC0bLEVolMg2Dn5eLSdlnbA4Nc82UU75X5w-Y9NrrdPlbH_ae_Sb6s5Y0IfDD48GuT0a8Ey26EvHmN0I6al2EZI3QghFV_RyyPqcyolY3d6Q8EeareH2u2_2teL9_-nO_F_e16Bd0fgvswpn_RGgRKcSvYAptOI8w</recordid><startdate>20210907</startdate><enddate>20210907</enddate><creator>Ooi, Soon-Keat</creator><creator>Sato, Shigeo</creator><creator>Tomomori-Sato, Chieri</creator><creator>Zhang, Ying</creator><creator>Wen, Zhihui</creator><creator>Banks, Charles A. S.</creator><creator>Washburn, Michael P.</creator><creator>Unruh, Jay R.</creator><creator>Florens, Laurence</creator><creator>Conaway, Ronald C.</creator><creator>Conaway, Joan W.</creator><general>National Academy of Sciences</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0001-7568-2585</orcidid><orcidid>https://orcid.org/0000-0003-3077-4990</orcidid><orcidid>https://orcid.org/0000-0002-9310-6650</orcidid><orcidid>https://orcid.org/0000-0002-2786-0663</orcidid><orcidid>https://orcid.org/0000-0002-0404-2940</orcidid></search><sort><creationdate>20210907</creationdate><title>Multiple roles for PARP1 in ALC1-dependent nucleosome remodeling</title><author>Ooi, Soon-Keat ; Sato, Shigeo ; Tomomori-Sato, Chieri ; Zhang, Ying ; Wen, Zhihui ; Banks, Charles A. S. ; Washburn, Michael P. ; Unruh, Jay R. ; Florens, Laurence ; Conaway, Ronald C. ; Conaway, Joan W.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c415t-5a0b4daebc8eec30ec036f443e9d790a6939a4399cac68e5d5dd86beb55f7053</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Biological Sciences</topic><topic>Cell Line, Tumor</topic><topic>Chromatin Assembly and Disassembly</topic><topic>DNA Helicases - metabolism</topic><topic>DNA Repair</topic><topic>DNA-Binding Proteins - metabolism</topic><topic>Humans</topic><topic>Nucleosomes - metabolism</topic><topic>Poly (ADP-Ribose) Polymerase-1 - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ooi, Soon-Keat</creatorcontrib><creatorcontrib>Sato, Shigeo</creatorcontrib><creatorcontrib>Tomomori-Sato, Chieri</creatorcontrib><creatorcontrib>Zhang, Ying</creatorcontrib><creatorcontrib>Wen, Zhihui</creatorcontrib><creatorcontrib>Banks, Charles A. S.</creatorcontrib><creatorcontrib>Washburn, Michael P.</creatorcontrib><creatorcontrib>Unruh, Jay R.</creatorcontrib><creatorcontrib>Florens, Laurence</creatorcontrib><creatorcontrib>Conaway, Ronald C.</creatorcontrib><creatorcontrib>Conaway, Joan W.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ooi, Soon-Keat</au><au>Sato, Shigeo</au><au>Tomomori-Sato, Chieri</au><au>Zhang, Ying</au><au>Wen, Zhihui</au><au>Banks, Charles A. S.</au><au>Washburn, Michael P.</au><au>Unruh, Jay R.</au><au>Florens, Laurence</au><au>Conaway, Ronald C.</au><au>Conaway, Joan W.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Multiple roles for PARP1 in ALC1-dependent nucleosome remodeling</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>2021-09-07</date><risdate>2021</risdate><volume>118</volume><issue>36</issue><spage>1</spage><epage>7</epage><pages>1-7</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><abstract>The SNF2 family ATPase Amplified in Liver Cancer 1 (ALC1) is the only chromatin remodeling enzyme with a poly(ADP-ribose) (PAR) binding macrodomain. ALC1 functions together with poly(ADP-ribose) polymerase PARP1 to remodel nucleosomes. Activation of ALC1 cryptic ATPase activity and the subsequent nucleosome remodeling requires binding of its macrodomain to PAR chains synthesized by PARP1 and NAD⁺. A key question is whether PARP1 has a role(s) in ALC1-dependent nucleosome remodeling beyond simply synthesizing the PAR chains needed to activate the ALC1 ATPase. Here, we identify PARP1 separation-of-function mutants that activate ALC1 ATPase but do not support nucleosome remodeling by ALC1. Investigation of these mutants has revealed multiple functions for PARP1 in ALC1-dependent nucleosome remodeling and provides insights into its multifaceted role in chromatin remodeling.</abstract><cop>United States</cop><pub>National Academy of Sciences</pub><pmid>34465625</pmid><doi>10.1073/pnas.2107277118</doi><tpages>7</tpages><orcidid>https://orcid.org/0000-0001-7568-2585</orcidid><orcidid>https://orcid.org/0000-0003-3077-4990</orcidid><orcidid>https://orcid.org/0000-0002-9310-6650</orcidid><orcidid>https://orcid.org/0000-0002-2786-0663</orcidid><orcidid>https://orcid.org/0000-0002-0404-2940</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0027-8424 |
| ispartof | Proceedings of the National Academy of Sciences - PNAS, 2021-09, Vol.118 (36), p.1-7 |
| issn | 0027-8424 1091-6490 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_8433585 |
| source | Jstor Complete Legacy; MEDLINE; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | Biological Sciences Cell Line, Tumor Chromatin Assembly and Disassembly DNA Helicases - metabolism DNA Repair DNA-Binding Proteins - metabolism Humans Nucleosomes - metabolism Poly (ADP-Ribose) Polymerase-1 - metabolism |
| title | Multiple roles for PARP1 in ALC1-dependent nucleosome remodeling |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-03T08%3A15%3A07IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-jstor_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Multiple%20roles%20for%20PARP1%20in%20ALC1-dependent%20nucleosome%20remodeling&rft.jtitle=Proceedings%20of%20the%20National%20Academy%20of%20Sciences%20-%20PNAS&rft.au=Ooi,%20Soon-Keat&rft.date=2021-09-07&rft.volume=118&rft.issue=36&rft.spage=1&rft.epage=7&rft.pages=1-7&rft.issn=0027-8424&rft.eissn=1091-6490&rft_id=info:doi/10.1073/pnas.2107277118&rft_dat=%3Cjstor_pubme%3E27075416%3C/jstor_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2568255674&rft_id=info:pmid/34465625&rft_jstor_id=27075416&rfr_iscdi=true |