Development of a nanobody-based ELISA for the detection of the insecticides cyantraniliprole and chlorantraniliprole in soil and the vegetable bok choy
Cyantraniliprole and chlorantraniliprole are anthranilic diamide insecticides acting on ryanodine receptors. In this study, two camel-derived nanobodies (Nbs, named C1 and C2) recognizing cyantraniliprole as well as chlorantraniliprole were generated. C1-based enzyme-linked immunosorbent assays (ELI...
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| Veröffentlicht in: | Analytical and bioanalytical chemistry 2021-04, Vol.413 (9), p.2503-2511 |
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| creator | Xu, Bojie Wang, Kai Vasylieva, Natalia Zhou, Hang Xue, Xianle Wang, Baomin Li, Qing X. Hammock, Bruce D. Xu, Ting |
| description | Cyantraniliprole and chlorantraniliprole are anthranilic diamide insecticides acting on ryanodine receptors. In this study, two camel-derived nanobodies (Nbs, named C1 and C2) recognizing cyantraniliprole as well as chlorantraniliprole were generated. C1-based enzyme-linked immunosorbent assays (ELISAs) for the detection of the two insecticides were developed. The half-maximum signal inhibition concentrations (IC
50
) of cyantraniliprole and chlorantraniliprole by ELISA were 1.2 and 1.5 ng mL
−1
, respectively. This assay was employed to detect these two insecticides in soil and vegetables. The average recoveries of cyantraniliprole from both bok choy (
Brassica chinensis
L.) and soil samples were 90–129%, while those of chlorantraniliprole were in a range of 89–120%. The insecticide residues in soil and bok choy, which were collected from plots sprayed with cyantraniliprole and chlorantraniliprole, were simultaneously detected by the resulting ELISA and a high-performance liquid chromatography (HPLC) method, showing a satisfactory correlation. Higher concentrations of chlorantraniliprole than cyantraniliprole were detected in soil and vegetables, which indicates the longer persistence of chlorantraniliprole in the environment. |
| doi_str_mv | 10.1007/s00216-021-03205-x |
| format | Article |
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50
) of cyantraniliprole and chlorantraniliprole by ELISA were 1.2 and 1.5 ng mL
−1
, respectively. This assay was employed to detect these two insecticides in soil and vegetables. The average recoveries of cyantraniliprole from both bok choy (
Brassica chinensis
L.) and soil samples were 90–129%, while those of chlorantraniliprole were in a range of 89–120%. The insecticide residues in soil and bok choy, which were collected from plots sprayed with cyantraniliprole and chlorantraniliprole, were simultaneously detected by the resulting ELISA and a high-performance liquid chromatography (HPLC) method, showing a satisfactory correlation. Higher concentrations of chlorantraniliprole than cyantraniliprole were detected in soil and vegetables, which indicates the longer persistence of chlorantraniliprole in the environment.</description><identifier>ISSN: 1618-2642</identifier><identifier>EISSN: 1618-2650</identifier><identifier>DOI: 10.1007/s00216-021-03205-x</identifier><identifier>PMID: 33580830</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Analysis ; Analytical Chemistry ; Biochemistry ; Brassica ; Brassica - chemistry ; Characterization and Evaluation of Materials ; Chemistry ; Chemistry and Materials Science ; Chinese cabbage ; Enzyme-linked immunosorbent assay ; Enzyme-Linked Immunosorbent Assay - methods ; Food Science ; High performance liquid chromatography ; Immunoassays ; Insecticides ; Insecticides - analysis ; Laboratory Medicine ; Liquid chromatography ; Methods ; Monitoring/Environmental Analysis ; Nanobodies ; ortho-Aminobenzoates - analysis ; Physiological aspects ; Plant-soil relationships ; Pyrazoles - analysis ; Research Paper ; Ryanodine receptors ; Soil - chemistry ; Soil Pollutants - analysis ; Soils ; Vegetables ; Vegetables - chemistry</subject><ispartof>Analytical and bioanalytical chemistry, 2021-04, Vol.413 (9), p.2503-2511</ispartof><rights>Springer-Verlag GmbH Germany, part of Springer Nature 2021</rights><rights>COPYRIGHT 2021 Springer</rights><rights>Springer-Verlag GmbH Germany, part of Springer Nature 2021.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c550t-3ba17ddc70f3f61d59accd7f1b1f1af4f5ea7d66900c386c4e2097a41873c40b3</citedby><cites>FETCH-LOGICAL-c550t-3ba17ddc70f3f61d59accd7f1b1f1af4f5ea7d66900c386c4e2097a41873c40b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00216-021-03205-x$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00216-021-03205-x$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>230,314,780,784,885,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33580830$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Xu, Bojie</creatorcontrib><creatorcontrib>Wang, Kai</creatorcontrib><creatorcontrib>Vasylieva, Natalia</creatorcontrib><creatorcontrib>Zhou, Hang</creatorcontrib><creatorcontrib>Xue, Xianle</creatorcontrib><creatorcontrib>Wang, Baomin</creatorcontrib><creatorcontrib>Li, Qing X.</creatorcontrib><creatorcontrib>Hammock, Bruce D.</creatorcontrib><creatorcontrib>Xu, Ting</creatorcontrib><title>Development of a nanobody-based ELISA for the detection of the insecticides cyantraniliprole and chlorantraniliprole in soil and the vegetable bok choy</title><title>Analytical and bioanalytical chemistry</title><addtitle>Anal Bioanal Chem</addtitle><addtitle>Anal Bioanal Chem</addtitle><description>Cyantraniliprole and chlorantraniliprole are anthranilic diamide insecticides acting on ryanodine receptors. In this study, two camel-derived nanobodies (Nbs, named C1 and C2) recognizing cyantraniliprole as well as chlorantraniliprole were generated. C1-based enzyme-linked immunosorbent assays (ELISAs) for the detection of the two insecticides were developed. The half-maximum signal inhibition concentrations (IC
50
) of cyantraniliprole and chlorantraniliprole by ELISA were 1.2 and 1.5 ng mL
−1
, respectively. This assay was employed to detect these two insecticides in soil and vegetables. The average recoveries of cyantraniliprole from both bok choy (
Brassica chinensis
L.) and soil samples were 90–129%, while those of chlorantraniliprole were in a range of 89–120%. The insecticide residues in soil and bok choy, which were collected from plots sprayed with cyantraniliprole and chlorantraniliprole, were simultaneously detected by the resulting ELISA and a high-performance liquid chromatography (HPLC) method, showing a satisfactory correlation. Higher concentrations of chlorantraniliprole than cyantraniliprole were detected in soil and vegetables, which indicates the longer persistence of chlorantraniliprole in the environment.</description><subject>Analysis</subject><subject>Analytical Chemistry</subject><subject>Biochemistry</subject><subject>Brassica</subject><subject>Brassica - chemistry</subject><subject>Characterization and Evaluation of Materials</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Chinese cabbage</subject><subject>Enzyme-linked immunosorbent assay</subject><subject>Enzyme-Linked Immunosorbent Assay - methods</subject><subject>Food Science</subject><subject>High performance liquid chromatography</subject><subject>Immunoassays</subject><subject>Insecticides</subject><subject>Insecticides - analysis</subject><subject>Laboratory Medicine</subject><subject>Liquid chromatography</subject><subject>Methods</subject><subject>Monitoring/Environmental Analysis</subject><subject>Nanobodies</subject><subject>ortho-Aminobenzoates - analysis</subject><subject>Physiological aspects</subject><subject>Plant-soil relationships</subject><subject>Pyrazoles - analysis</subject><subject>Research Paper</subject><subject>Ryanodine receptors</subject><subject>Soil - chemistry</subject><subject>Soil Pollutants - analysis</subject><subject>Soils</subject><subject>Vegetables</subject><subject>Vegetables - 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titles)</collection><jtitle>Analytical and bioanalytical chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Xu, Bojie</au><au>Wang, Kai</au><au>Vasylieva, Natalia</au><au>Zhou, Hang</au><au>Xue, Xianle</au><au>Wang, Baomin</au><au>Li, Qing X.</au><au>Hammock, Bruce D.</au><au>Xu, Ting</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Development of a nanobody-based ELISA for the detection of the insecticides cyantraniliprole and chlorantraniliprole in soil and the vegetable bok choy</atitle><jtitle>Analytical and bioanalytical chemistry</jtitle><stitle>Anal Bioanal Chem</stitle><addtitle>Anal Bioanal Chem</addtitle><date>2021-04-01</date><risdate>2021</risdate><volume>413</volume><issue>9</issue><spage>2503</spage><epage>2511</epage><pages>2503-2511</pages><issn>1618-2642</issn><eissn>1618-2650</eissn><abstract>Cyantraniliprole and chlorantraniliprole are anthranilic diamide insecticides acting on ryanodine receptors. In this study, two camel-derived nanobodies (Nbs, named C1 and C2) recognizing cyantraniliprole as well as chlorantraniliprole were generated. C1-based enzyme-linked immunosorbent assays (ELISAs) for the detection of the two insecticides were developed. The half-maximum signal inhibition concentrations (IC
50
) of cyantraniliprole and chlorantraniliprole by ELISA were 1.2 and 1.5 ng mL
−1
, respectively. This assay was employed to detect these two insecticides in soil and vegetables. The average recoveries of cyantraniliprole from both bok choy (
Brassica chinensis
L.) and soil samples were 90–129%, while those of chlorantraniliprole were in a range of 89–120%. The insecticide residues in soil and bok choy, which were collected from plots sprayed with cyantraniliprole and chlorantraniliprole, were simultaneously detected by the resulting ELISA and a high-performance liquid chromatography (HPLC) method, showing a satisfactory correlation. Higher concentrations of chlorantraniliprole than cyantraniliprole were detected in soil and vegetables, which indicates the longer persistence of chlorantraniliprole in the environment.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>33580830</pmid><doi>10.1007/s00216-021-03205-x</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; SpringerNature Journals |
| subjects | Analysis Analytical Chemistry Biochemistry Brassica Brassica - chemistry Characterization and Evaluation of Materials Chemistry Chemistry and Materials Science Chinese cabbage Enzyme-linked immunosorbent assay Enzyme-Linked Immunosorbent Assay - methods Food Science High performance liquid chromatography Immunoassays Insecticides Insecticides - analysis Laboratory Medicine Liquid chromatography Methods Monitoring/Environmental Analysis Nanobodies ortho-Aminobenzoates - analysis Physiological aspects Plant-soil relationships Pyrazoles - analysis Research Paper Ryanodine receptors Soil - chemistry Soil Pollutants - analysis Soils Vegetables Vegetables - chemistry |
| title | Development of a nanobody-based ELISA for the detection of the insecticides cyantraniliprole and chlorantraniliprole in soil and the vegetable bok choy |
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