Neuronal odor coding in the larval sensory cone of Anopheles coluzzii: Complex responses from a simple system

Anopheles mosquitoes are the sole vectors of malaria. Although adult females are directly responsible for disease transmission and accordingly have been extensively studied, the survival of pre-adult larval stages is vital. Mosquito larvae utilize a spectrum of chemosensory and other cues to navigate their aquatic habitats to avoid predators and search for food. Here we examine larval olfactory responses, in which the peripheral components are associated with the antennal sensory cone. Larval behavior and sensory cone responses to volatile stimuli in Anopheles coluzzii demonstrate the sensory cone is particularly tuned to alcohols, thiazoles, and heterocyclics, and these responses can be assigned to discrete groups of sensory cone neurons with distinctive profiles. These studies reveal that the anopheline larvae actively sample volatile odors above their aquatic habitats via a highly sophisticated olfactory system that is sensitive to a broad range of compounds with significant behavioral relevance. [Display omitted] •Anopheline larvae detect, discriminate, and respond to volatile odors•The larval sensory cone has a broad response profile•Responses are linked to discrete neuron groups with distinctive sensitivity profiles•Larval behavioral responses to volatiles that robustly activate the sensory cone Sun et al. investigate larval sensory cone and behavioral responses to volatile stimuli in Anopheles coluzzii. They find that malaria mosquito larvae actively sample volatile odors above their aquatic habitats via a highly sophisticated olfactory system that is sensitive to a broad range of compounds with significant behavioral relevance.