The impact of using culture media containing granulocyte-macrophage colony-stimulating factor on live birth rates in patients with a history of embryonic developmental arrest in previous in vitro fertilization cycles
Objective: To investigate the effect of using culture media containing granulocyte-macrophage colony-stimulating factor (GM-CSF) on embryological data and reproductive outcomes in patients with early embryonic developmental arrest. Material and Methods: Retrospective case-control study. A total of 3...
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| Veröffentlicht in: | Journal of the Turkish German Gynecological Association 2021-09, Vol.22 (3), p.181-186 |
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| creator | Sipahi, Mehmet Mümüşoğlu, Sezcan Coşkun Akçay, Neslihan Sever, Ayten Yeğenoğlu, Hande Bozdağ, Gürkan Karakoç Sökmensüer, Lale |
| description | Objective: To investigate the effect of using culture media containing granulocyte-macrophage colony-stimulating factor (GM-CSF) on embryological data and reproductive outcomes in patients with early embryonic developmental arrest.
Material and Methods: Retrospective case-control study. A total of 39 patients, whose embryos were incubated with culture media containing GM-CSF due to embryonic developmental arrest in two previous in vitro fertilization (IVF) cycles in-between January 2016 and November 2017 at Hacettepe University IVF Center, were enrolled. Control group was generated among patients with first IVF attempts due to tubal factor in the same time period. All embryos in the control group were incubated with single step culture medium (without GM-CSF). For the control group selection, matching was done 1:2 ratio considering female age, body mass index, number of M-II oocyte retrieved, and number of embryo transferred (n=80).
Results: Demographic features and embryological data were comparable between two groups. Number of fertilized oocytes (2-pronuclear) was 3.7±2.0 in GM-CSF group and 3.9±2.5 in the control (p=0.576). Overall, number of embryos transferred (1.3±0.5 vs 1.3±0.5, respectively) and blastocyst transfer rate (67.6% vs 59.2%, respectively; p=0.401) were similar. For the reproductive outcomes, implantation rate (32.3% vs 33.1%, respectively; p=0.937), clinical pregnancy rate (33.3% vs 32.5%, respectively; p=0.770), and live birth rate (25.2% vs 26.2%, respectively; p=0.943) were similar.
Conclusion: Using GM-CSF-containing culture media in patients with two previous failed IVF attempts due to embryonic developmental arrest might rectify embryological data and reproductive outcomes. To make solid conclusion further randomized controlled trials are warranted. (J Turk Ger Gynecol Assoc 2021; 22: 181-6) |
| doi_str_mv | 10.4274/jtgga.galenos.2021.2020.0168 |
| format | Article |
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Material and Methods: Retrospective case-control study. A total of 39 patients, whose embryos were incubated with culture media containing GM-CSF due to embryonic developmental arrest in two previous in vitro fertilization (IVF) cycles in-between January 2016 and November 2017 at Hacettepe University IVF Center, were enrolled. Control group was generated among patients with first IVF attempts due to tubal factor in the same time period. All embryos in the control group were incubated with single step culture medium (without GM-CSF). For the control group selection, matching was done 1:2 ratio considering female age, body mass index, number of M-II oocyte retrieved, and number of embryo transferred (n=80).
Results: Demographic features and embryological data were comparable between two groups. Number of fertilized oocytes (2-pronuclear) was 3.7±2.0 in GM-CSF group and 3.9±2.5 in the control (p=0.576). Overall, number of embryos transferred (1.3±0.5 vs 1.3±0.5, respectively) and blastocyst transfer rate (67.6% vs 59.2%, respectively; p=0.401) were similar. For the reproductive outcomes, implantation rate (32.3% vs 33.1%, respectively; p=0.937), clinical pregnancy rate (33.3% vs 32.5%, respectively; p=0.770), and live birth rate (25.2% vs 26.2%, respectively; p=0.943) were similar.
Conclusion: Using GM-CSF-containing culture media in patients with two previous failed IVF attempts due to embryonic developmental arrest might rectify embryological data and reproductive outcomes. To make solid conclusion further randomized controlled trials are warranted. (J Turk Ger Gynecol Assoc 2021; 22: 181-6)</description><identifier>ISSN: 1309-0399</identifier><identifier>EISSN: 1309-0380</identifier><identifier>DOI: 10.4274/jtgga.galenos.2021.2020.0168</identifier><identifier>PMID: 33631877</identifier><language>eng</language><publisher>Istanbul: Turkish-German Gynecological Association</publisher><subject>Embryos ; Granulocytes ; In vitro fertilization ; Original Investigation ; Tıp</subject><ispartof>Journal of the Turkish German Gynecological Association, 2021-09, Vol.22 (3), p.181-186</ispartof><rights>2021. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the associated terms available at http://www.jtgga.org/copyright</rights><rights>Copyright 2021 by the Turkish-German Gynecological Education and Research Foundation 2021</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><orcidid>0000-0002-6679-9623 ; 0000-0001-7000-7580 ; 0000-0001-7067-7322 ; 0000-0002-7821-6798 ; 0000-0001-8344-9863 ; 0000-0001-6840-9329</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8420754/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8420754/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,53766,53768</link.rule.ids></links><search><contributor>Ünlü,Cihat</contributor><creatorcontrib>Sipahi, Mehmet</creatorcontrib><creatorcontrib>Mümüşoğlu, Sezcan</creatorcontrib><creatorcontrib>Coşkun Akçay, Neslihan</creatorcontrib><creatorcontrib>Sever, Ayten</creatorcontrib><creatorcontrib>Yeğenoğlu, Hande</creatorcontrib><creatorcontrib>Bozdağ, Gürkan</creatorcontrib><creatorcontrib>Karakoç Sökmensüer, Lale</creatorcontrib><title>The impact of using culture media containing granulocyte-macrophage colony-stimulating factor on live birth rates in patients with a history of embryonic developmental arrest in previous in vitro fertilization cycles</title><title>Journal of the Turkish German Gynecological Association</title><description>Objective: To investigate the effect of using culture media containing granulocyte-macrophage colony-stimulating factor (GM-CSF) on embryological data and reproductive outcomes in patients with early embryonic developmental arrest.
Material and Methods: Retrospective case-control study. A total of 39 patients, whose embryos were incubated with culture media containing GM-CSF due to embryonic developmental arrest in two previous in vitro fertilization (IVF) cycles in-between January 2016 and November 2017 at Hacettepe University IVF Center, were enrolled. Control group was generated among patients with first IVF attempts due to tubal factor in the same time period. All embryos in the control group were incubated with single step culture medium (without GM-CSF). For the control group selection, matching was done 1:2 ratio considering female age, body mass index, number of M-II oocyte retrieved, and number of embryo transferred (n=80).
Results: Demographic features and embryological data were comparable between two groups. Number of fertilized oocytes (2-pronuclear) was 3.7±2.0 in GM-CSF group and 3.9±2.5 in the control (p=0.576). Overall, number of embryos transferred (1.3±0.5 vs 1.3±0.5, respectively) and blastocyst transfer rate (67.6% vs 59.2%, respectively; p=0.401) were similar. For the reproductive outcomes, implantation rate (32.3% vs 33.1%, respectively; p=0.937), clinical pregnancy rate (33.3% vs 32.5%, respectively; p=0.770), and live birth rate (25.2% vs 26.2%, respectively; p=0.943) were similar.
Conclusion: Using GM-CSF-containing culture media in patients with two previous failed IVF attempts due to embryonic developmental arrest might rectify embryological data and reproductive outcomes. To make solid conclusion further randomized controlled trials are warranted. (J Turk Ger Gynecol Assoc 2021; 22: 181-6)</description><subject>Embryos</subject><subject>Granulocytes</subject><subject>In vitro fertilization</subject><subject>Original Investigation</subject><subject>Tıp</subject><issn>1309-0399</issn><issn>1309-0380</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNpVks1u3CAUha2qVROleQekdtGNJ-CfsZGqSlGappFG6iZdowu-9hBhcAFP5T5pH6d4ZpSqG0Dcj8PhcrLsA6Obqmiqm-c4DLAZwKB1YVPQgq0D3VC2bV9ll6ykPKdlS1-_rDm_yK5D0JJWVVPVnJdvs4uy3JasbZrL7M_THokeJ1CRuJ7MQduBqNnE2SMZsdNAlLMRtF0Lgwc7G6eWiPkIyrtpDwMmwji75CHqcTYQV7JPgs4TZ4nRByRS-7gnHiIGoi2ZEoQ2BvJLp20gex0SvawOcJR-cVYr0uEBjZvGBIIh4D2GeDzs8aDdfBQ66Ogd6dFHbfTvpJouVIsyGN5lb3owAa_P81X24-v90923fPf94fHudperirGYd7ykPeWl7BUDLCh2UmLdsVryvpFUdhIBoad1K5uGsZ5DJbnkbcGbru63UF5ln0-60yxTv1Ry68GIyesR_CIcaPF_xeq9GNxBtFVBm7pKAu_PAt79nNMbxbObvU2eRVG3rKDbulypjydKdwip20Zb_Ac-frm_3QlWF23RJvTTCU3_E4LH_sUMo2JNkTimSJxTJNYUrQMVa4rKv1Bnx7w</recordid><startdate>20210901</startdate><enddate>20210901</enddate><creator>Sipahi, Mehmet</creator><creator>Mümüşoğlu, Sezcan</creator><creator>Coşkun Akçay, Neslihan</creator><creator>Sever, Ayten</creator><creator>Yeğenoğlu, Hande</creator><creator>Bozdağ, Gürkan</creator><creator>Karakoç Sökmensüer, Lale</creator><general>Turkish-German Gynecological Association</general><general>Galenos Publishing House</general><general>Galenos Publishing</general><scope>AAYXX</scope><scope>CITATION</scope><scope>IEBAR</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>EDSIH</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>M0S</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-6679-9623</orcidid><orcidid>https://orcid.org/0000-0001-7000-7580</orcidid><orcidid>https://orcid.org/0000-0001-7067-7322</orcidid><orcidid>https://orcid.org/0000-0002-7821-6798</orcidid><orcidid>https://orcid.org/0000-0001-8344-9863</orcidid><orcidid>https://orcid.org/0000-0001-6840-9329</orcidid></search><sort><creationdate>20210901</creationdate><title>The impact of using culture media containing granulocyte-macrophage colony-stimulating factor on live birth rates in patients with a history of embryonic developmental arrest in previous in vitro fertilization cycles</title><author>Sipahi, Mehmet ; Mümüşoğlu, Sezcan ; Coşkun Akçay, Neslihan ; Sever, Ayten ; Yeğenoğlu, Hande ; Bozdağ, Gürkan ; Karakoç Sökmensüer, Lale</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c411t-d930f093bfc1ae20edbbe5d15b9f7b0bdbeaeaf058b7711f9a4b9b98297d5f6a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Embryos</topic><topic>Granulocytes</topic><topic>In vitro fertilization</topic><topic>Original Investigation</topic><topic>Tıp</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sipahi, Mehmet</creatorcontrib><creatorcontrib>Mümüşoğlu, Sezcan</creatorcontrib><creatorcontrib>Coşkun Akçay, Neslihan</creatorcontrib><creatorcontrib>Sever, Ayten</creatorcontrib><creatorcontrib>Yeğenoğlu, Hande</creatorcontrib><creatorcontrib>Bozdağ, Gürkan</creatorcontrib><creatorcontrib>Karakoç Sökmensüer, Lale</creatorcontrib><collection>CrossRef</collection><collection>Idealonline online kütüphane - Journals</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Turkey Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of the Turkish German Gynecological Association</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sipahi, Mehmet</au><au>Mümüşoğlu, Sezcan</au><au>Coşkun Akçay, Neslihan</au><au>Sever, Ayten</au><au>Yeğenoğlu, Hande</au><au>Bozdağ, Gürkan</au><au>Karakoç Sökmensüer, Lale</au><au>Ünlü,Cihat</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The impact of using culture media containing granulocyte-macrophage colony-stimulating factor on live birth rates in patients with a history of embryonic developmental arrest in previous in vitro fertilization cycles</atitle><jtitle>Journal of the Turkish German Gynecological Association</jtitle><date>2021-09-01</date><risdate>2021</risdate><volume>22</volume><issue>3</issue><spage>181</spage><epage>186</epage><pages>181-186</pages><issn>1309-0399</issn><eissn>1309-0380</eissn><abstract>Objective: To investigate the effect of using culture media containing granulocyte-macrophage colony-stimulating factor (GM-CSF) on embryological data and reproductive outcomes in patients with early embryonic developmental arrest.
Material and Methods: Retrospective case-control study. A total of 39 patients, whose embryos were incubated with culture media containing GM-CSF due to embryonic developmental arrest in two previous in vitro fertilization (IVF) cycles in-between January 2016 and November 2017 at Hacettepe University IVF Center, were enrolled. Control group was generated among patients with first IVF attempts due to tubal factor in the same time period. All embryos in the control group were incubated with single step culture medium (without GM-CSF). For the control group selection, matching was done 1:2 ratio considering female age, body mass index, number of M-II oocyte retrieved, and number of embryo transferred (n=80).
Results: Demographic features and embryological data were comparable between two groups. Number of fertilized oocytes (2-pronuclear) was 3.7±2.0 in GM-CSF group and 3.9±2.5 in the control (p=0.576). Overall, number of embryos transferred (1.3±0.5 vs 1.3±0.5, respectively) and blastocyst transfer rate (67.6% vs 59.2%, respectively; p=0.401) were similar. For the reproductive outcomes, implantation rate (32.3% vs 33.1%, respectively; p=0.937), clinical pregnancy rate (33.3% vs 32.5%, respectively; p=0.770), and live birth rate (25.2% vs 26.2%, respectively; p=0.943) were similar.
Conclusion: Using GM-CSF-containing culture media in patients with two previous failed IVF attempts due to embryonic developmental arrest might rectify embryological data and reproductive outcomes. To make solid conclusion further randomized controlled trials are warranted. (J Turk Ger Gynecol Assoc 2021; 22: 181-6)</abstract><cop>Istanbul</cop><pub>Turkish-German Gynecological Association</pub><pmid>33631877</pmid><doi>10.4274/jtgga.galenos.2021.2020.0168</doi><tpages>6</tpages><orcidid>https://orcid.org/0000-0002-6679-9623</orcidid><orcidid>https://orcid.org/0000-0001-7000-7580</orcidid><orcidid>https://orcid.org/0000-0001-7067-7322</orcidid><orcidid>https://orcid.org/0000-0002-7821-6798</orcidid><orcidid>https://orcid.org/0000-0001-8344-9863</orcidid><orcidid>https://orcid.org/0000-0001-6840-9329</orcidid><oa>free_for_read</oa></addata></record> |
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| source | Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central |
| subjects | Embryos Granulocytes In vitro fertilization Original Investigation Tıp |
| title | The impact of using culture media containing granulocyte-macrophage colony-stimulating factor on live birth rates in patients with a history of embryonic developmental arrest in previous in vitro fertilization cycles |
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