Real‐Time and Label‐Free Measurement of Deubiquitinase Activity with a MspA Nanopore

Covalently attaching ubiquitin (Ub) to cellular proteins as a post‐translational modification can result in altered function of modified proteins. Enzymes regulating Ub as a post‐translational modification, such as ligases and deubiquitinases, are challenging to characterize in part due to the low t...

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Veröffentlicht in:	Chembiochem : a European journal of chemical biology 2021-09, Vol.22 (17), p.2688-2692
Hauptverfasser:	Shorkey, Spencer A., Du, Jiale, Pham, Ryan, Strieter, Eric R., Chen, Min
Format:	Artikel
Sprache:	eng
Schlagworte:	deubiquitinase activity assay Deubiquitinating Enzymes - analysis Deubiquitinating Enzymes - metabolism Drug screening Enzymes Humans MspA nanopore Nanopores Proteasomes Proteins sensing Translation Ubiquitin Ubiquitin - analysis Ubiquitin - chemistry Ubiquitin - metabolism Ubiquitin Thiolesterase - analysis Ubiquitin Thiolesterase - metabolism
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