Uptake and Toxicity of Respirable Carbon-Rich Uranium-Bearing Particles: Insights into the Role of Particulates in Uranium Toxicity
Particulate matter (PM) presents an environmental health risk for communities residing close to uranium (U) mine sites. However, the role of the particulate form of U on its cellular toxicity is still poorly understood. Here, we investigated the cellular uptake and toxicity of C-rich U-bearing parti...
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| Veröffentlicht in: | Environmental science & technology 2021-07, Vol.55 (14), p.9949-9957 |
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| creator | El Hayek, Eliane Medina, Sebastian Guo, Jimin Noureddine, Achraf Zychowski, Katherine E Hunter, Russell Velasco, Carmen A Wiesse, Marco Maestas-Olguin, Angelea Brinker, C. Jeffrey Brearley, Adrian Spilde, Michael Howard, Tamara Lauer, Fredine T Herbert, Guy Ali, Abdul Mehdi Burchiel, Scott Campen, Matthew J Cerrato, José M |
| description | Particulate matter (PM) presents an environmental health risk for communities residing close to uranium (U) mine sites. However, the role of the particulate form of U on its cellular toxicity is still poorly understood. Here, we investigated the cellular uptake and toxicity of C-rich U-bearing particles as a model organic particulate containing uranyl citrate over a range of environmentally relevant concentrations of U (0–445 μM). The cytotoxicity of C-rich U-bearing particles in human epithelial cells (A549) was U-dose-dependent. No cytotoxic effects were detected with soluble U doses. Carbon-rich U-bearing particles with a wide size distribution ( |
| doi_str_mv | 10.1021/acs.est.1c01205 |
| format | Article |
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Jeffrey ; Brearley, Adrian ; Spilde, Michael ; Howard, Tamara ; Lauer, Fredine T ; Herbert, Guy ; Ali, Abdul Mehdi ; Burchiel, Scott ; Campen, Matthew J ; Cerrato, José M</creator><creatorcontrib>El Hayek, Eliane ; Medina, Sebastian ; Guo, Jimin ; Noureddine, Achraf ; Zychowski, Katherine E ; Hunter, Russell ; Velasco, Carmen A ; Wiesse, Marco ; Maestas-Olguin, Angelea ; Brinker, C. Jeffrey ; Brearley, Adrian ; Spilde, Michael ; Howard, Tamara ; Lauer, Fredine T ; Herbert, Guy ; Ali, Abdul Mehdi ; Burchiel, Scott ; Campen, Matthew J ; Cerrato, José M</creatorcontrib><description>Particulate matter (PM) presents an environmental health risk for communities residing close to uranium (U) mine sites. However, the role of the particulate form of U on its cellular toxicity is still poorly understood. Here, we investigated the cellular uptake and toxicity of C-rich U-bearing particles as a model organic particulate containing uranyl citrate over a range of environmentally relevant concentrations of U (0–445 μM). The cytotoxicity of C-rich U-bearing particles in human epithelial cells (A549) was U-dose-dependent. No cytotoxic effects were detected with soluble U doses. Carbon-rich U-bearing particles with a wide size distribution (<10 μm) presented 2.7 times higher U uptake into cells than the particles with a narrow size distribution (<1 μm) at 100 μM U concentration. TEM-EDS analysis identified the intracellular translocation of clusters of C-rich U-bearing particles. The accumulation of C-rich U-bearing particles induced DNA damage and cytotoxicity as indicated by the increased phosphorylation of the histone H2AX and cell death, respectively. These findings reveal the toxicity of the particulate form of U under environmentally relevant heterogeneous size distributions. Our study opens new avenues for future investigations on the health impacts resulting from environmental exposures to the particulate form of U near mine sites.</description><identifier>ISSN: 0013-936X</identifier><identifier>EISSN: 1520-5851</identifier><identifier>DOI: 10.1021/acs.est.1c01205</identifier><identifier>PMID: 34235927</identifier><language>eng</language><publisher>United States: American Chemical Society</publisher><subject>Carbon ; Cell death ; Citric acid ; Coal ; Cytotoxicity ; Damage accumulation ; DNA damage ; Dust - analysis ; Ecotoxicology and Public Health ; Environmental health ; Epithelial cells ; Epithelium ; Health risks ; Histone H2A ; Humans ; Particle size ; Particle size distribution ; Particulate emissions ; Particulate matter ; Particulate Matter - analysis ; Particulate Matter - toxicity ; Particulates ; Phosphorylation ; Size distribution ; Toxicity ; Translocation ; Uranium ; Uranium - analysis ; Uranium - toxicity</subject><ispartof>Environmental science & technology, 2021-07, Vol.55 (14), p.9949-9957</ispartof><rights>2021 American Chemical Society</rights><rights>Copyright American Chemical Society Jul 20, 2021</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a457t-b3529c11d0253ff452bda5933ac9af46f92b82393d78e8494b42983f762a0e9d3</citedby><cites>FETCH-LOGICAL-a457t-b3529c11d0253ff452bda5933ac9af46f92b82393d78e8494b42983f762a0e9d3</cites><orcidid>0000-0003-1129-2419 ; 0000-0001-9530-5963 ; 0000-0002-7145-9324 ; 0000-0002-2473-6376</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/acs.est.1c01205$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/acs.est.1c01205$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>230,314,776,780,881,2752,27053,27901,27902,56713,56763</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34235927$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>El Hayek, Eliane</creatorcontrib><creatorcontrib>Medina, Sebastian</creatorcontrib><creatorcontrib>Guo, Jimin</creatorcontrib><creatorcontrib>Noureddine, Achraf</creatorcontrib><creatorcontrib>Zychowski, Katherine E</creatorcontrib><creatorcontrib>Hunter, Russell</creatorcontrib><creatorcontrib>Velasco, Carmen A</creatorcontrib><creatorcontrib>Wiesse, Marco</creatorcontrib><creatorcontrib>Maestas-Olguin, Angelea</creatorcontrib><creatorcontrib>Brinker, C. Jeffrey</creatorcontrib><creatorcontrib>Brearley, Adrian</creatorcontrib><creatorcontrib>Spilde, Michael</creatorcontrib><creatorcontrib>Howard, Tamara</creatorcontrib><creatorcontrib>Lauer, Fredine T</creatorcontrib><creatorcontrib>Herbert, Guy</creatorcontrib><creatorcontrib>Ali, Abdul Mehdi</creatorcontrib><creatorcontrib>Burchiel, Scott</creatorcontrib><creatorcontrib>Campen, Matthew J</creatorcontrib><creatorcontrib>Cerrato, José M</creatorcontrib><title>Uptake and Toxicity of Respirable Carbon-Rich Uranium-Bearing Particles: Insights into the Role of Particulates in Uranium Toxicity</title><title>Environmental science & technology</title><addtitle>Environ. Sci. Technol</addtitle><description>Particulate matter (PM) presents an environmental health risk for communities residing close to uranium (U) mine sites. However, the role of the particulate form of U on its cellular toxicity is still poorly understood. 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Our study opens new avenues for future investigations on the health impacts resulting from environmental exposures to the particulate form of U near mine sites.</description><subject>Carbon</subject><subject>Cell death</subject><subject>Citric acid</subject><subject>Coal</subject><subject>Cytotoxicity</subject><subject>Damage accumulation</subject><subject>DNA damage</subject><subject>Dust - analysis</subject><subject>Ecotoxicology and Public Health</subject><subject>Environmental health</subject><subject>Epithelial cells</subject><subject>Epithelium</subject><subject>Health risks</subject><subject>Histone H2A</subject><subject>Humans</subject><subject>Particle size</subject><subject>Particle size distribution</subject><subject>Particulate emissions</subject><subject>Particulate matter</subject><subject>Particulate Matter - analysis</subject><subject>Particulate Matter - toxicity</subject><subject>Particulates</subject><subject>Phosphorylation</subject><subject>Size distribution</subject><subject>Toxicity</subject><subject>Translocation</subject><subject>Uranium</subject><subject>Uranium - analysis</subject><subject>Uranium - toxicity</subject><issn>0013-936X</issn><issn>1520-5851</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kU1vEzEURS0EoqGwZocssUST-jMzZoEEUWkrVSqKGomd9cbjSVwmdmp7ULvmj-MoaQSLrrx455735IvQe0qmlDB6BiZNbcpTaghlRL5AEyoZqWQj6Us0IYTySvHZzxP0JqU7QgjjpHmNTrhgXCpWT9Cf5TbDL4vBd_g2PDjj8iMOPV7YtHUR2sHiOcQ2-GrhzBovI3g3bqpvFqLzK_wDYnZmsOkzvvLJrdY5YedzwHlt8SKUdHHtoXGAbHfTJ8lx31v0qoch2XeH9xQtv5_fzi-r65uLq_nX6wqErHPVcsmUobQjTPK-F5K1HUjFORgFvZj1irUN44p3dWMboUQrmGp4X88YEKs6foq-7L3bsd3YzlifIwx6G90G4qMO4PT_E-_WehV-60ZQToUogo8HQQz3Y_l3fRfG6MvNmklZM6kaxgp1tqdMDClF2x83UKJ3renSmt6lD62VxId_DzvyTzUV4NMe2CWPO5_T_QVO-qUA</recordid><startdate>20210720</startdate><enddate>20210720</enddate><creator>El Hayek, Eliane</creator><creator>Medina, Sebastian</creator><creator>Guo, Jimin</creator><creator>Noureddine, Achraf</creator><creator>Zychowski, Katherine E</creator><creator>Hunter, Russell</creator><creator>Velasco, Carmen A</creator><creator>Wiesse, Marco</creator><creator>Maestas-Olguin, Angelea</creator><creator>Brinker, C. 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Jeffrey</au><au>Brearley, Adrian</au><au>Spilde, Michael</au><au>Howard, Tamara</au><au>Lauer, Fredine T</au><au>Herbert, Guy</au><au>Ali, Abdul Mehdi</au><au>Burchiel, Scott</au><au>Campen, Matthew J</au><au>Cerrato, José M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Uptake and Toxicity of Respirable Carbon-Rich Uranium-Bearing Particles: Insights into the Role of Particulates in Uranium Toxicity</atitle><jtitle>Environmental science & technology</jtitle><addtitle>Environ. Sci. Technol</addtitle><date>2021-07-20</date><risdate>2021</risdate><volume>55</volume><issue>14</issue><spage>9949</spage><epage>9957</epage><pages>9949-9957</pages><issn>0013-936X</issn><eissn>1520-5851</eissn><abstract>Particulate matter (PM) presents an environmental health risk for communities residing close to uranium (U) mine sites. However, the role of the particulate form of U on its cellular toxicity is still poorly understood. Here, we investigated the cellular uptake and toxicity of C-rich U-bearing particles as a model organic particulate containing uranyl citrate over a range of environmentally relevant concentrations of U (0–445 μM). The cytotoxicity of C-rich U-bearing particles in human epithelial cells (A549) was U-dose-dependent. No cytotoxic effects were detected with soluble U doses. Carbon-rich U-bearing particles with a wide size distribution (<10 μm) presented 2.7 times higher U uptake into cells than the particles with a narrow size distribution (<1 μm) at 100 μM U concentration. TEM-EDS analysis identified the intracellular translocation of clusters of C-rich U-bearing particles. The accumulation of C-rich U-bearing particles induced DNA damage and cytotoxicity as indicated by the increased phosphorylation of the histone H2AX and cell death, respectively. These findings reveal the toxicity of the particulate form of U under environmentally relevant heterogeneous size distributions. Our study opens new avenues for future investigations on the health impacts resulting from environmental exposures to the particulate form of U near mine sites.</abstract><cop>United States</cop><pub>American Chemical Society</pub><pmid>34235927</pmid><doi>10.1021/acs.est.1c01205</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0003-1129-2419</orcidid><orcidid>https://orcid.org/0000-0001-9530-5963</orcidid><orcidid>https://orcid.org/0000-0002-7145-9324</orcidid><orcidid>https://orcid.org/0000-0002-2473-6376</orcidid><oa>free_for_read</oa></addata></record> |
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| ispartof | Environmental science & technology, 2021-07, Vol.55 (14), p.9949-9957 |
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| source | MEDLINE; ACS Journals: American Chemical Society Web Editions |
| subjects | Carbon Cell death Citric acid Coal Cytotoxicity Damage accumulation DNA damage Dust - analysis Ecotoxicology and Public Health Environmental health Epithelial cells Epithelium Health risks Histone H2A Humans Particle size Particle size distribution Particulate emissions Particulate matter Particulate Matter - analysis Particulate Matter - toxicity Particulates Phosphorylation Size distribution Toxicity Translocation Uranium Uranium - analysis Uranium - toxicity |
| title | Uptake and Toxicity of Respirable Carbon-Rich Uranium-Bearing Particles: Insights into the Role of Particulates in Uranium Toxicity |
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