Molecular and Immunohistochemical Cognizance of HPV16 in Oral Leukoplakia, Oral Squamous Cell Carcinoma and Oropharyngeal Squamous Cell Carcinoma
Prior studies have established the carcinogenic role of HPV16 and also demonstrated its unique biological behavior in cervical and oropharyngeal squamous cell carcinoma (OPSCC) but its role in oral leukoplakia (OL) and oral squamous cell carcinoma (OSCC) is not well explored. Therefore, in the prese...
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| Veröffentlicht in: | Head & neck pathology (Totowa, N.J.) N.J.), 2021-09, Vol.15 (3), p.882-892 |
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| container_title | Head & neck pathology (Totowa, N.J.) |
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| creator | Sivakumar, N. Narwal, Anjali Kamboj, Mala Devi, Anju Kumar, Sanjay Bhardwaj, Rashmi |
| description | Prior studies have established the carcinogenic role of HPV16 and also demonstrated its unique biological behavior in cervical and oropharyngeal squamous cell carcinoma (OPSCC) but its role in oral leukoplakia (OL) and oral squamous cell carcinoma (OSCC) is not well explored. Therefore, in the present study, we assessed HPV16 prevalence using PCR and Anti-HPV16 antibodies for the first time and correlated its biological behavior using p16
INK4a
and Ki67 proliferation index (PI) in OL, OSCC, and OPSCC. This study included 63 subjects comprising of 25 OL, 26 OSCC, and 12 OPSCC cases. Exfoliated cells were collected and processed for PCR followed by immunohistochemistry with primary antibodies p16
INK4a
, Anti-HPV16, and Ki67. The expressions were evaluated and statistical analysis included Chi-square and Spearman’s test. Cumulatively 37% (OL—7%, OSCC—14% & OPSCC—16%) of cases showed positive PCR expression. PCR positivity was observed to be significantly higher (p 0.00) in OPSCC (9/12) than OSCC (9/26) and OL (5/25) cases. Overall immunohistochemical expression of p16
INK4a
, Anti-HPV16, and Ki67 were significantly (p 0.02) higher in HPV16 (PCR) positive cases. HPV16 + OSCC cases showed higher grades of p16
INK4a
and Ki67 expression. We have demonstrated a prevalence of HPV16 in OL, OSCC, and OPSCC through PCR, which may be concluded as a gold standard for the detection of HPV16 DNA. |
| doi_str_mv | 10.1007/s12105-021-01309-5 |
| format | Article |
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INK4a
and Ki67 proliferation index (PI) in OL, OSCC, and OPSCC. This study included 63 subjects comprising of 25 OL, 26 OSCC, and 12 OPSCC cases. Exfoliated cells were collected and processed for PCR followed by immunohistochemistry with primary antibodies p16
INK4a
, Anti-HPV16, and Ki67. The expressions were evaluated and statistical analysis included Chi-square and Spearman’s test. Cumulatively 37% (OL—7%, OSCC—14% & OPSCC—16%) of cases showed positive PCR expression. PCR positivity was observed to be significantly higher (p 0.00) in OPSCC (9/12) than OSCC (9/26) and OL (5/25) cases. Overall immunohistochemical expression of p16
INK4a
, Anti-HPV16, and Ki67 were significantly (p 0.02) higher in HPV16 (PCR) positive cases. HPV16 + OSCC cases showed higher grades of p16
INK4a
and Ki67 expression. We have demonstrated a prevalence of HPV16 in OL, OSCC, and OPSCC through PCR, which may be concluded as a gold standard for the detection of HPV16 DNA.</description><identifier>ISSN: 1936-0568</identifier><identifier>ISSN: 1936-055X</identifier><identifier>EISSN: 1936-0568</identifier><identifier>DOI: 10.1007/s12105-021-01309-5</identifier><identifier>PMID: 33646558</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>Adult ; Aged ; Dentistry ; Female ; Head and Neck Neoplasms - virology ; Human papillomavirus 16 ; Humans ; Immunohistochemistry ; India ; Leukoplakia, Oral - virology ; Male ; Medicine ; Medicine & Public Health ; Middle Aged ; Oral and Maxillofacial Surgery ; Original Paper ; Otorhinolaryngology ; Papillomavirus Infections - complications ; Papillomavirus Infections - epidemiology ; Pathology ; Polymerase Chain Reaction ; Prevalence ; Squamous Cell Carcinoma of Head and Neck - virology</subject><ispartof>Head & neck pathology (Totowa, N.J.), 2021-09, Vol.15 (3), p.882-892</ispartof><rights>The Author(s), under exclusive licence to Springer Science+Business Media, LLC part of Springer Nature 2021</rights><rights>2021. The Author(s), under exclusive licence to Springer Science+Business Media, LLC part of Springer Nature.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c446t-a99931938bf77df1d6a9b1c04779e8d91515cbe75d1d7660abfc9dc8350b66793</citedby><cites>FETCH-LOGICAL-c446t-a99931938bf77df1d6a9b1c04779e8d91515cbe75d1d7660abfc9dc8350b66793</cites><orcidid>0000-0002-7961-1132</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8385043/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8385043/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,41464,42533,51294,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33646558$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sivakumar, N.</creatorcontrib><creatorcontrib>Narwal, Anjali</creatorcontrib><creatorcontrib>Kamboj, Mala</creatorcontrib><creatorcontrib>Devi, Anju</creatorcontrib><creatorcontrib>Kumar, Sanjay</creatorcontrib><creatorcontrib>Bhardwaj, Rashmi</creatorcontrib><title>Molecular and Immunohistochemical Cognizance of HPV16 in Oral Leukoplakia, Oral Squamous Cell Carcinoma and Oropharyngeal Squamous Cell Carcinoma</title><title>Head & neck pathology (Totowa, N.J.)</title><addtitle>Head and Neck Pathol</addtitle><addtitle>Head Neck Pathol</addtitle><description>Prior studies have established the carcinogenic role of HPV16 and also demonstrated its unique biological behavior in cervical and oropharyngeal squamous cell carcinoma (OPSCC) but its role in oral leukoplakia (OL) and oral squamous cell carcinoma (OSCC) is not well explored. Therefore, in the present study, we assessed HPV16 prevalence using PCR and Anti-HPV16 antibodies for the first time and correlated its biological behavior using p16
INK4a
and Ki67 proliferation index (PI) in OL, OSCC, and OPSCC. This study included 63 subjects comprising of 25 OL, 26 OSCC, and 12 OPSCC cases. Exfoliated cells were collected and processed for PCR followed by immunohistochemistry with primary antibodies p16
INK4a
, Anti-HPV16, and Ki67. The expressions were evaluated and statistical analysis included Chi-square and Spearman’s test. Cumulatively 37% (OL—7%, OSCC—14% & OPSCC—16%) of cases showed positive PCR expression. PCR positivity was observed to be significantly higher (p 0.00) in OPSCC (9/12) than OSCC (9/26) and OL (5/25) cases. Overall immunohistochemical expression of p16
INK4a
, Anti-HPV16, and Ki67 were significantly (p 0.02) higher in HPV16 (PCR) positive cases. HPV16 + OSCC cases showed higher grades of p16
INK4a
and Ki67 expression. We have demonstrated a prevalence of HPV16 in OL, OSCC, and OPSCC through PCR, which may be concluded as a gold standard for the detection of HPV16 DNA.</description><subject>Adult</subject><subject>Aged</subject><subject>Dentistry</subject><subject>Female</subject><subject>Head and Neck Neoplasms - virology</subject><subject>Human papillomavirus 16</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>India</subject><subject>Leukoplakia, Oral - virology</subject><subject>Male</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Middle Aged</subject><subject>Oral and Maxillofacial Surgery</subject><subject>Original Paper</subject><subject>Otorhinolaryngology</subject><subject>Papillomavirus Infections - complications</subject><subject>Papillomavirus Infections - epidemiology</subject><subject>Pathology</subject><subject>Polymerase Chain Reaction</subject><subject>Prevalence</subject><subject>Squamous Cell Carcinoma of Head and Neck - virology</subject><issn>1936-0568</issn><issn>1936-055X</issn><issn>1936-0568</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9Uc1O3DAQtlAroMALcKjyAA2M17EdXyqhCArSVlupwNVyHGfXS2Kn9gapfYu-Me6GrpZDexprvp8Zz4fQOYYLDMAvI55hoDnMcA6YgMjpATrGgrAcKCvf7b2P0IcY1wAMeAGH6IgQVjBKy2P0-6vvjB47FTLlmuyu70fnVzZuvF6Z3mrVZZVfOvtLOW0y32a33x4xy6zLFiFhczM--aFTT1Z9mjrff4yq92PMKtMlrQraOt-rrfsi-GGlwk-3NP9mnqL3reqiOXutJ-jh5vq-us3niy931dU810XBNrkSQpD0wbJuOW9a3DAlaqyh4FyYshGYYqprw2mDG84YqLrVotEloVAzxgU5QZ8n32Gse9No4zZpfzkE26cVpVdWvkWcXcmlf5YlKSkUJBnMJgMdfIzBtDstBvknIDkFJFNAchuQpEn0cX_qTvI3kUQgEyEmKB0qyLUfg0uX-J_tCzIKnx8</recordid><startdate>20210901</startdate><enddate>20210901</enddate><creator>Sivakumar, N.</creator><creator>Narwal, Anjali</creator><creator>Kamboj, Mala</creator><creator>Devi, Anju</creator><creator>Kumar, Sanjay</creator><creator>Bhardwaj, Rashmi</creator><general>Springer US</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-7961-1132</orcidid></search><sort><creationdate>20210901</creationdate><title>Molecular and Immunohistochemical Cognizance of HPV16 in Oral Leukoplakia, Oral Squamous Cell Carcinoma and Oropharyngeal Squamous Cell Carcinoma</title><author>Sivakumar, N. ; Narwal, Anjali ; Kamboj, Mala ; Devi, Anju ; Kumar, Sanjay ; Bhardwaj, Rashmi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c446t-a99931938bf77df1d6a9b1c04779e8d91515cbe75d1d7660abfc9dc8350b66793</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Adult</topic><topic>Aged</topic><topic>Dentistry</topic><topic>Female</topic><topic>Head and Neck Neoplasms - virology</topic><topic>Human papillomavirus 16</topic><topic>Humans</topic><topic>Immunohistochemistry</topic><topic>India</topic><topic>Leukoplakia, Oral - virology</topic><topic>Male</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Middle Aged</topic><topic>Oral and Maxillofacial Surgery</topic><topic>Original Paper</topic><topic>Otorhinolaryngology</topic><topic>Papillomavirus Infections - complications</topic><topic>Papillomavirus Infections - epidemiology</topic><topic>Pathology</topic><topic>Polymerase Chain Reaction</topic><topic>Prevalence</topic><topic>Squamous Cell Carcinoma of Head and Neck - virology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sivakumar, N.</creatorcontrib><creatorcontrib>Narwal, Anjali</creatorcontrib><creatorcontrib>Kamboj, Mala</creatorcontrib><creatorcontrib>Devi, Anju</creatorcontrib><creatorcontrib>Kumar, Sanjay</creatorcontrib><creatorcontrib>Bhardwaj, Rashmi</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Head & neck pathology (Totowa, N.J.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sivakumar, N.</au><au>Narwal, Anjali</au><au>Kamboj, Mala</au><au>Devi, Anju</au><au>Kumar, Sanjay</au><au>Bhardwaj, Rashmi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular and Immunohistochemical Cognizance of HPV16 in Oral Leukoplakia, Oral Squamous Cell Carcinoma and Oropharyngeal Squamous Cell Carcinoma</atitle><jtitle>Head & neck pathology (Totowa, N.J.)</jtitle><stitle>Head and Neck Pathol</stitle><addtitle>Head Neck Pathol</addtitle><date>2021-09-01</date><risdate>2021</risdate><volume>15</volume><issue>3</issue><spage>882</spage><epage>892</epage><pages>882-892</pages><issn>1936-0568</issn><issn>1936-055X</issn><eissn>1936-0568</eissn><abstract>Prior studies have established the carcinogenic role of HPV16 and also demonstrated its unique biological behavior in cervical and oropharyngeal squamous cell carcinoma (OPSCC) but its role in oral leukoplakia (OL) and oral squamous cell carcinoma (OSCC) is not well explored. Therefore, in the present study, we assessed HPV16 prevalence using PCR and Anti-HPV16 antibodies for the first time and correlated its biological behavior using p16
INK4a
and Ki67 proliferation index (PI) in OL, OSCC, and OPSCC. This study included 63 subjects comprising of 25 OL, 26 OSCC, and 12 OPSCC cases. Exfoliated cells were collected and processed for PCR followed by immunohistochemistry with primary antibodies p16
INK4a
, Anti-HPV16, and Ki67. The expressions were evaluated and statistical analysis included Chi-square and Spearman’s test. Cumulatively 37% (OL—7%, OSCC—14% & OPSCC—16%) of cases showed positive PCR expression. PCR positivity was observed to be significantly higher (p 0.00) in OPSCC (9/12) than OSCC (9/26) and OL (5/25) cases. Overall immunohistochemical expression of p16
INK4a
, Anti-HPV16, and Ki67 were significantly (p 0.02) higher in HPV16 (PCR) positive cases. HPV16 + OSCC cases showed higher grades of p16
INK4a
and Ki67 expression. We have demonstrated a prevalence of HPV16 in OL, OSCC, and OPSCC through PCR, which may be concluded as a gold standard for the detection of HPV16 DNA.</abstract><cop>New York</cop><pub>Springer US</pub><pmid>33646558</pmid><doi>10.1007/s12105-021-01309-5</doi><tpages>11</tpages><orcidid>https://orcid.org/0000-0002-7961-1132</orcidid><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; SpringerLink Journals - AutoHoldings |
| subjects | Adult Aged Dentistry Female Head and Neck Neoplasms - virology Human papillomavirus 16 Humans Immunohistochemistry India Leukoplakia, Oral - virology Male Medicine Medicine & Public Health Middle Aged Oral and Maxillofacial Surgery Original Paper Otorhinolaryngology Papillomavirus Infections - complications Papillomavirus Infections - epidemiology Pathology Polymerase Chain Reaction Prevalence Squamous Cell Carcinoma of Head and Neck - virology |
| title | Molecular and Immunohistochemical Cognizance of HPV16 in Oral Leukoplakia, Oral Squamous Cell Carcinoma and Oropharyngeal Squamous Cell Carcinoma |
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