Sexual differences in mitochondrial and related proteins in rat cerebral microvessels: A proteomic approach
Sex differences in mitochondrial numbers and function are present in large cerebral arteries, but it is unclear whether these differences extend to the microcirculation. We performed an assessment of mitochondria-related proteins in cerebral microvessels (MVs) isolated from young, male and female, S...
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| Veröffentlicht in: | Journal of cerebral blood flow and metabolism 2021-02, Vol.41 (2), p.397-412 |
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| container_title | Journal of cerebral blood flow and metabolism |
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| creator | Cikic, Sinisa Chandra, Partha K Harman, Jarrod C Rutkai, Ibolya Katakam, Prasad VG Guidry, Jessie J Gidday, Jeffrey M Busija, David W |
| description | Sex differences in mitochondrial numbers and function are present in large cerebral arteries, but it is unclear whether these differences extend to the microcirculation. We performed an assessment of mitochondria-related proteins in cerebral microvessels (MVs) isolated from young, male and female, Sprague-Dawley rats. MVs composed of arterioles, capillaries, and venules were isolated from the cerebrum and used to perform a 3 versus 3 quantitative, multiplexed proteomics experiment utilizing tandem mass tags (TMT), coupled with liquid chromatography/mass spectrometry (LC/MS). MS data and bioinformatic analyses were performed using Proteome Discoverer version 2.2 and Ingenuity Pathway Analysis. We identified a total of 1969 proteins, of which 1871 were quantified by TMT labels. Sixty-four proteins were expressed significantly (p |
| doi_str_mv | 10.1177/0271678X20915127 |
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We performed an assessment of mitochondria-related proteins in cerebral microvessels (MVs) isolated from young, male and female, Sprague-Dawley rats. MVs composed of arterioles, capillaries, and venules were isolated from the cerebrum and used to perform a 3 versus 3 quantitative, multiplexed proteomics experiment utilizing tandem mass tags (TMT), coupled with liquid chromatography/mass spectrometry (LC/MS). MS data and bioinformatic analyses were performed using Proteome Discoverer version 2.2 and Ingenuity Pathway Analysis. We identified a total of 1969 proteins, of which 1871 were quantified by TMT labels. Sixty-four proteins were expressed significantly (p < 0.05) higher in female samples compared with male samples. Females expressed more mitochondrial proteins involved in energy production, mitochondrial membrane structure, anti-oxidant enzyme proteins, and those involved in fatty acid oxidation. Conversely, males had higher expression levels of mitochondria-destructive proteins. Our findings reveal, for the first time, the full extent of sexual dimorphism in the mitochondrial metabolic protein profiles of MVs, which may contribute to sex-dependent cerebrovascular and neurological pathologies.</description><identifier>ISSN: 0271-678X</identifier><identifier>EISSN: 1559-7016</identifier><identifier>DOI: 10.1177/0271678X20915127</identifier><identifier>PMID: 32241204</identifier><language>eng</language><publisher>London, England: SAGE Publications</publisher><subject>Animals ; Computational Biology - methods ; Female ; Male ; Microvessels - metabolism ; Mitochondria - metabolism ; Original ; Proteomics - methods ; Rats ; Rats, Sprague-Dawley</subject><ispartof>Journal of cerebral blood flow and metabolism, 2021-02, Vol.41 (2), p.397-412</ispartof><rights>The Author(s) 2020</rights><rights>The Author(s) 2020 2020 International Society for Cerebral Blood Flow and Metabolism</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c500t-92cb2ff4905ab4147c97315d4f6619ad0ba8e4dbca17aebe8808b8489fbd52163</citedby><cites>FETCH-LOGICAL-c500t-92cb2ff4905ab4147c97315d4f6619ad0ba8e4dbca17aebe8808b8489fbd52163</cites><orcidid>0000-0002-4396-3996 ; 0000-0002-5601-0430 ; 0000-0002-4249-5916</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8370005/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8370005/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,21819,27924,27925,43621,43622,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32241204$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Cikic, Sinisa</creatorcontrib><creatorcontrib>Chandra, Partha K</creatorcontrib><creatorcontrib>Harman, Jarrod C</creatorcontrib><creatorcontrib>Rutkai, Ibolya</creatorcontrib><creatorcontrib>Katakam, Prasad VG</creatorcontrib><creatorcontrib>Guidry, Jessie J</creatorcontrib><creatorcontrib>Gidday, Jeffrey M</creatorcontrib><creatorcontrib>Busija, David W</creatorcontrib><title>Sexual differences in mitochondrial and related proteins in rat cerebral microvessels: A proteomic approach</title><title>Journal of cerebral blood flow and metabolism</title><addtitle>J Cereb Blood Flow Metab</addtitle><description>Sex differences in mitochondrial numbers and function are present in large cerebral arteries, but it is unclear whether these differences extend to the microcirculation. We performed an assessment of mitochondria-related proteins in cerebral microvessels (MVs) isolated from young, male and female, Sprague-Dawley rats. MVs composed of arterioles, capillaries, and venules were isolated from the cerebrum and used to perform a 3 versus 3 quantitative, multiplexed proteomics experiment utilizing tandem mass tags (TMT), coupled with liquid chromatography/mass spectrometry (LC/MS). MS data and bioinformatic analyses were performed using Proteome Discoverer version 2.2 and Ingenuity Pathway Analysis. We identified a total of 1969 proteins, of which 1871 were quantified by TMT labels. Sixty-four proteins were expressed significantly (p < 0.05) higher in female samples compared with male samples. Females expressed more mitochondrial proteins involved in energy production, mitochondrial membrane structure, anti-oxidant enzyme proteins, and those involved in fatty acid oxidation. Conversely, males had higher expression levels of mitochondria-destructive proteins. Our findings reveal, for the first time, the full extent of sexual dimorphism in the mitochondrial metabolic protein profiles of MVs, which may contribute to sex-dependent cerebrovascular and neurological pathologies.</description><subject>Animals</subject><subject>Computational Biology - methods</subject><subject>Female</subject><subject>Male</subject><subject>Microvessels - metabolism</subject><subject>Mitochondria - metabolism</subject><subject>Original</subject><subject>Proteomics - methods</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><issn>0271-678X</issn><issn>1559-7016</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kc1P3DAQxS0E6m4X7pxQjlzS2k4cOz0goVW_pJV6gEq9Wf6YsF4Se2snCP57vCxFUImTLb_fezOeQeiU4E-EcP4ZU04aLv5Q3BJGKD9Ac8JYW3JMmkM038nlTp-hjyltMMaiYuwDmlWU1oTieo5ur-B-Un1hXddBBG8gFc4XgxuDWQdvo8ui8raI0KsRbLGNYQTnn6ioxsJkl44ZGpyJ4Q5Sgj59KS73YMivhdrmuzLrY3TUqT7ByfO5QL-_fb1e_ihXv77_XF6uSsMwHsuWGk27rm4xU7omNTctrwizddc0pFUWayWgttoowhVoEAILLWrRdtoySppqgS72udtJD2AN-DE3KLfRDSo-yKCcfKt4t5Y34U6KiucZsRxw_hwQw98J0igHlwz0vfIQpiRpJRrKm5bRjOI9mj-fUoTupQzBcrcj-f-OsuXsdXsvhn9LyUC5B5K6AbkJU_R5XO8HPgJQaZyy</recordid><startdate>20210201</startdate><enddate>20210201</enddate><creator>Cikic, Sinisa</creator><creator>Chandra, Partha K</creator><creator>Harman, Jarrod C</creator><creator>Rutkai, Ibolya</creator><creator>Katakam, Prasad VG</creator><creator>Guidry, Jessie J</creator><creator>Gidday, Jeffrey M</creator><creator>Busija, David W</creator><general>SAGE Publications</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-4396-3996</orcidid><orcidid>https://orcid.org/0000-0002-5601-0430</orcidid><orcidid>https://orcid.org/0000-0002-4249-5916</orcidid></search><sort><creationdate>20210201</creationdate><title>Sexual differences in mitochondrial and related proteins in rat cerebral microvessels: A proteomic approach</title><author>Cikic, Sinisa ; Chandra, Partha K ; Harman, Jarrod C ; Rutkai, Ibolya ; Katakam, Prasad VG ; Guidry, Jessie J ; Gidday, Jeffrey M ; Busija, David W</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c500t-92cb2ff4905ab4147c97315d4f6619ad0ba8e4dbca17aebe8808b8489fbd52163</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Animals</topic><topic>Computational Biology - methods</topic><topic>Female</topic><topic>Male</topic><topic>Microvessels - metabolism</topic><topic>Mitochondria - metabolism</topic><topic>Original</topic><topic>Proteomics - methods</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cikic, Sinisa</creatorcontrib><creatorcontrib>Chandra, Partha K</creatorcontrib><creatorcontrib>Harman, Jarrod C</creatorcontrib><creatorcontrib>Rutkai, Ibolya</creatorcontrib><creatorcontrib>Katakam, Prasad VG</creatorcontrib><creatorcontrib>Guidry, Jessie J</creatorcontrib><creatorcontrib>Gidday, Jeffrey M</creatorcontrib><creatorcontrib>Busija, David W</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of cerebral blood flow and metabolism</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cikic, Sinisa</au><au>Chandra, Partha K</au><au>Harman, Jarrod C</au><au>Rutkai, Ibolya</au><au>Katakam, Prasad VG</au><au>Guidry, Jessie J</au><au>Gidday, Jeffrey M</au><au>Busija, David W</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Sexual differences in mitochondrial and related proteins in rat cerebral microvessels: A proteomic approach</atitle><jtitle>Journal of cerebral blood flow and metabolism</jtitle><addtitle>J Cereb Blood Flow Metab</addtitle><date>2021-02-01</date><risdate>2021</risdate><volume>41</volume><issue>2</issue><spage>397</spage><epage>412</epage><pages>397-412</pages><issn>0271-678X</issn><eissn>1559-7016</eissn><abstract>Sex differences in mitochondrial numbers and function are present in large cerebral arteries, but it is unclear whether these differences extend to the microcirculation. We performed an assessment of mitochondria-related proteins in cerebral microvessels (MVs) isolated from young, male and female, Sprague-Dawley rats. MVs composed of arterioles, capillaries, and venules were isolated from the cerebrum and used to perform a 3 versus 3 quantitative, multiplexed proteomics experiment utilizing tandem mass tags (TMT), coupled with liquid chromatography/mass spectrometry (LC/MS). MS data and bioinformatic analyses were performed using Proteome Discoverer version 2.2 and Ingenuity Pathway Analysis. We identified a total of 1969 proteins, of which 1871 were quantified by TMT labels. Sixty-four proteins were expressed significantly (p < 0.05) higher in female samples compared with male samples. Females expressed more mitochondrial proteins involved in energy production, mitochondrial membrane structure, anti-oxidant enzyme proteins, and those involved in fatty acid oxidation. Conversely, males had higher expression levels of mitochondria-destructive proteins. 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| subjects | Animals Computational Biology - methods Female Male Microvessels - metabolism Mitochondria - metabolism Original Proteomics - methods Rats Rats, Sprague-Dawley |
| title | Sexual differences in mitochondrial and related proteins in rat cerebral microvessels: A proteomic approach |
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