Therapeutic Effect of Murine Bone Marrow-Derived Mesenchymal Stromal/Stem Cells and Human Placental Extract on Testicular Toxicity Resulting from Doxorubicin in Rats
Oncotherapeutics like doxorubicin can affect male gonads; as a result, it leads to infertility. This work was conducted to demonstrate the toxic effects of doxorubicin on testes of male albino rats. Fifty male albino rats aged 5-7 weeks were used in this study. The animals were randomly separated in...
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| creator | AbdRabou, Mervat Ahmed Mehany, Ahmed B. M. Farrag, Islam M. Belal, Amany Abdelzaher, Othman F. El-Sharkawy, Abdou Abd El-Azez, Asmaa M. EL-Sharkawy, Salah M. Al Badawi, Manal H. |
| description | Oncotherapeutics like doxorubicin can affect male gonads; as a result, it leads to infertility. This work was conducted to demonstrate the toxic effects of doxorubicin on testes of male albino rats. Fifty male albino rats aged 5-7 weeks were used in this study. The animals were randomly separated into 5 sets (each set containing ten rats). Group I received saline (i.p.) for 4 weeks. Group II was given doxorubicin (DOX), 5 mg/kg BW (i.p.) once/week for 4 weeks. Groups III and IV were treated in the same way as the DOX group, left for one week without medication, and then injected with mesenchymal stromal cells (MSCs) or human placental extract (HPE) therapy in a single dose of 5×106 in 200 ml PRP/week or 40 μl placental extract for 4 weeks via the caudal vein. Group V rats were treated in the same way as the DOX group also, left for one week without medication, and then injected with MSC+HPE. A significant decrease in serum testosterone, FSH, and LH levels was observed in rats treated with DOX compared to the control group. A significant elevation was recorded in rats treated with DOX+MSC or DOX+HPE when compared with the DOX group only. Rats that were given MSC+HPE after DOX intoxication showed a significant increase in hormone levels when compared to rats treated with either MSC or HPE. Light and electron microscopic examinations revealed that DOX intoxication initiated degenerative and necrotic changes in seminiferous tubules associated with partial or complete cessation of spermatogenesis. These effects were reversed by the effect of MSC or HPE. Coadministration of MSC and HPE even showed further improvement. Finally, we can say that doxorubicin has a deleterious impact on rat testes; however, therapeutic effects can be induced through MSC and/or HPE administration. |
| doi_str_mv | 10.1155/2021/9979670 |
| format | Article |
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M. ; Farrag, Islam M. ; Belal, Amany ; Abdelzaher, Othman F. ; El-Sharkawy, Abdou ; Abd El-Azez, Asmaa M. ; EL-Sharkawy, Salah M. ; Al Badawi, Manal H.</creator><contributor>Baralla, Elena ; Elena Baralla</contributor><creatorcontrib>AbdRabou, Mervat Ahmed ; Mehany, Ahmed B. M. ; Farrag, Islam M. ; Belal, Amany ; Abdelzaher, Othman F. ; El-Sharkawy, Abdou ; Abd El-Azez, Asmaa M. ; EL-Sharkawy, Salah M. ; Al Badawi, Manal H. ; Baralla, Elena ; Elena Baralla</creatorcontrib><description>Oncotherapeutics like doxorubicin can affect male gonads; as a result, it leads to infertility. This work was conducted to demonstrate the toxic effects of doxorubicin on testes of male albino rats. Fifty male albino rats aged 5-7 weeks were used in this study. The animals were randomly separated into 5 sets (each set containing ten rats). Group I received saline (i.p.) for 4 weeks. Group II was given doxorubicin (DOX), 5 mg/kg BW (i.p.) once/week for 4 weeks. Groups III and IV were treated in the same way as the DOX group, left for one week without medication, and then injected with mesenchymal stromal cells (MSCs) or human placental extract (HPE) therapy in a single dose of 5×106 in 200 ml PRP/week or 40 μl placental extract for 4 weeks via the caudal vein. Group V rats were treated in the same way as the DOX group also, left for one week without medication, and then injected with MSC+HPE. A significant decrease in serum testosterone, FSH, and LH levels was observed in rats treated with DOX compared to the control group. A significant elevation was recorded in rats treated with DOX+MSC or DOX+HPE when compared with the DOX group only. Rats that were given MSC+HPE after DOX intoxication showed a significant increase in hormone levels when compared to rats treated with either MSC or HPE. Light and electron microscopic examinations revealed that DOX intoxication initiated degenerative and necrotic changes in seminiferous tubules associated with partial or complete cessation of spermatogenesis. These effects were reversed by the effect of MSC or HPE. Coadministration of MSC and HPE even showed further improvement. Finally, we can say that doxorubicin has a deleterious impact on rat testes; however, therapeutic effects can be induced through MSC and/or HPE administration.</description><identifier>ISSN: 2314-6133</identifier><identifier>EISSN: 2314-6141</identifier><identifier>DOI: 10.1155/2021/9979670</identifier><identifier>PMID: 34409109</identifier><language>eng</language><publisher>New York: Hindawi</publisher><subject>Adverse and side effects ; Antioxidants ; Apoptosis ; Bone marrow ; Bone marrow cells ; Cancer ; Cancer therapies ; Care and treatment ; Cell cycle ; Cell division ; Cellular therapy ; Chemical properties ; Chemotherapy ; Complications and side effects ; Connective tissue cells ; Cytokines ; Doxorubicin ; Drugs ; Enzymes ; Flow cytometry ; Follicle-stimulating hormone ; Gonads ; Growth factors ; Health aspects ; Hematopoietic stem cells ; Infertility ; Intoxication ; Laboratory animals ; Lipid peroxidation ; Luteinizing hormone ; Males ; Mesenchyme ; Oxidative stress ; Placenta ; Sperm ; Spermatogenesis ; Stem cell transplantation ; Stem cells ; Stromal cells ; Testes ; Testosterone ; Therapeutics, Experimental ; Toxicity ; Transplantation ; Tubules ; Tumors</subject><ispartof>BioMed research international, 2021, Vol.2021 (1), p.9979670-9979670</ispartof><rights>Copyright © 2021 Mervat Ahmed AbdRabou et al.</rights><rights>COPYRIGHT 2021 John Wiley & Sons, Inc.</rights><rights>Copyright © 2021 Mervat Ahmed AbdRabou et al. This is an open access article distributed under the Creative Commons Attribution License (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. https://creativecommons.org/licenses/by/4.0</rights><rights>Copyright © 2021 Mervat Ahmed AbdRabou et al. 2021</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c481t-9a0fc863a35896f4d46cb71295de136e8f5dad9017cf0d39f28fcf80a64e750b3</citedby><cites>FETCH-LOGICAL-c481t-9a0fc863a35896f4d46cb71295de136e8f5dad9017cf0d39f28fcf80a64e750b3</cites><orcidid>0000-0002-9304-5309 ; 0000-0003-4293-8446 ; 0000-0001-5775-0775 ; 0000-0003-1045-0163 ; 0000-0003-0101-2962 ; 0000-0002-8446-2653 ; 0000-0001-6031-8799 ; 0000-0002-9913-5380</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8367585/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8367585/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,4010,27900,27901,27902,53766,53768</link.rule.ids></links><search><contributor>Baralla, Elena</contributor><contributor>Elena Baralla</contributor><creatorcontrib>AbdRabou, Mervat Ahmed</creatorcontrib><creatorcontrib>Mehany, Ahmed B. M.</creatorcontrib><creatorcontrib>Farrag, Islam M.</creatorcontrib><creatorcontrib>Belal, Amany</creatorcontrib><creatorcontrib>Abdelzaher, Othman F.</creatorcontrib><creatorcontrib>El-Sharkawy, Abdou</creatorcontrib><creatorcontrib>Abd El-Azez, Asmaa M.</creatorcontrib><creatorcontrib>EL-Sharkawy, Salah M.</creatorcontrib><creatorcontrib>Al Badawi, Manal H.</creatorcontrib><title>Therapeutic Effect of Murine Bone Marrow-Derived Mesenchymal Stromal/Stem Cells and Human Placental Extract on Testicular Toxicity Resulting from Doxorubicin in Rats</title><title>BioMed research international</title><description>Oncotherapeutics like doxorubicin can affect male gonads; as a result, it leads to infertility. This work was conducted to demonstrate the toxic effects of doxorubicin on testes of male albino rats. Fifty male albino rats aged 5-7 weeks were used in this study. The animals were randomly separated into 5 sets (each set containing ten rats). Group I received saline (i.p.) for 4 weeks. Group II was given doxorubicin (DOX), 5 mg/kg BW (i.p.) once/week for 4 weeks. Groups III and IV were treated in the same way as the DOX group, left for one week without medication, and then injected with mesenchymal stromal cells (MSCs) or human placental extract (HPE) therapy in a single dose of 5×106 in 200 ml PRP/week or 40 μl placental extract for 4 weeks via the caudal vein. Group V rats were treated in the same way as the DOX group also, left for one week without medication, and then injected with MSC+HPE. A significant decrease in serum testosterone, FSH, and LH levels was observed in rats treated with DOX compared to the control group. A significant elevation was recorded in rats treated with DOX+MSC or DOX+HPE when compared with the DOX group only. Rats that were given MSC+HPE after DOX intoxication showed a significant increase in hormone levels when compared to rats treated with either MSC or HPE. Light and electron microscopic examinations revealed that DOX intoxication initiated degenerative and necrotic changes in seminiferous tubules associated with partial or complete cessation of spermatogenesis. These effects were reversed by the effect of MSC or HPE. Coadministration of MSC and HPE even showed further improvement. Finally, we can say that doxorubicin has a deleterious impact on rat testes; however, therapeutic effects can be induced through MSC and/or HPE administration.</description><subject>Adverse and side effects</subject><subject>Antioxidants</subject><subject>Apoptosis</subject><subject>Bone marrow</subject><subject>Bone marrow cells</subject><subject>Cancer</subject><subject>Cancer therapies</subject><subject>Care and treatment</subject><subject>Cell cycle</subject><subject>Cell division</subject><subject>Cellular therapy</subject><subject>Chemical properties</subject><subject>Chemotherapy</subject><subject>Complications and side effects</subject><subject>Connective tissue cells</subject><subject>Cytokines</subject><subject>Doxorubicin</subject><subject>Drugs</subject><subject>Enzymes</subject><subject>Flow cytometry</subject><subject>Follicle-stimulating hormone</subject><subject>Gonads</subject><subject>Growth factors</subject><subject>Health aspects</subject><subject>Hematopoietic stem cells</subject><subject>Infertility</subject><subject>Intoxication</subject><subject>Laboratory animals</subject><subject>Lipid peroxidation</subject><subject>Luteinizing hormone</subject><subject>Males</subject><subject>Mesenchyme</subject><subject>Oxidative stress</subject><subject>Placenta</subject><subject>Sperm</subject><subject>Spermatogenesis</subject><subject>Stem cell transplantation</subject><subject>Stem cells</subject><subject>Stromal cells</subject><subject>Testes</subject><subject>Testosterone</subject><subject>Therapeutics, Experimental</subject><subject>Toxicity</subject><subject>Transplantation</subject><subject>Tubules</subject><subject>Tumors</subject><issn>2314-6133</issn><issn>2314-6141</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>RHX</sourceid><sourceid>BENPR</sourceid><recordid>eNqFkt9qFDEUxgdRbKm98wEC3gi6bjKZZJIboW5XK3RR2vU6ZDMnuykzyTaZaXcfyPc0wy4VvdAQcgLnx3f-8BXFa4I_EMLYtMQlmUpZS17jZ8VpSUk14aQiz5_-lJ4U5ynd4XwE4Vjyl8UJrSosCZanxc_lBqLewtA7g-bWgulRsGgxROcBfQr5WegYw-PkEqJ7gAYtIIE3m32nW3Tbx5Dj9LaHDs2gbRPSvkFXQ6c9-t5qA77P2HzXRz0Ke7SElCsNrY5oGXbOuH6PbiANbe_8Gtkshy7DLsRhlXMe5Xuj-_SqeGF1m-D8GM-KH5_ny9nV5Prbl6-zi-uJqQTpJ1JjawSnmjIhua2aiptVTUrJGiCUg7Cs0Y3EpDYWN1TaUlhjBda8gprhFT0rPh50t8Oqg2ZsP-pWbaPrdNyroJ36M-PdRq3DgxKU10ywLPD2KBDD_ZBnVZ1LJi9GewhDUiXjpaCUSJnRN3-hd2GIPo83UpRyKkr6m1rrFpTzNoyrHEXVBZdc1BXD_6EEZXW2xNjc-wNlYkgpgn0ajGA12kmNdlJHO2X83QHfON_oR_dv-heM2Mjo</recordid><startdate>2021</startdate><enddate>2021</enddate><creator>AbdRabou, Mervat Ahmed</creator><creator>Mehany, Ahmed B. 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Fifty male albino rats aged 5-7 weeks were used in this study. The animals were randomly separated into 5 sets (each set containing ten rats). Group I received saline (i.p.) for 4 weeks. Group II was given doxorubicin (DOX), 5 mg/kg BW (i.p.) once/week for 4 weeks. Groups III and IV were treated in the same way as the DOX group, left for one week without medication, and then injected with mesenchymal stromal cells (MSCs) or human placental extract (HPE) therapy in a single dose of 5×106 in 200 ml PRP/week or 40 μl placental extract for 4 weeks via the caudal vein. Group V rats were treated in the same way as the DOX group also, left for one week without medication, and then injected with MSC+HPE. A significant decrease in serum testosterone, FSH, and LH levels was observed in rats treated with DOX compared to the control group. A significant elevation was recorded in rats treated with DOX+MSC or DOX+HPE when compared with the DOX group only. Rats that were given MSC+HPE after DOX intoxication showed a significant increase in hormone levels when compared to rats treated with either MSC or HPE. Light and electron microscopic examinations revealed that DOX intoxication initiated degenerative and necrotic changes in seminiferous tubules associated with partial or complete cessation of spermatogenesis. These effects were reversed by the effect of MSC or HPE. Coadministration of MSC and HPE even showed further improvement. 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| identifier | ISSN: 2314-6133 |
| ispartof | BioMed research international, 2021, Vol.2021 (1), p.9979670-9979670 |
| issn | 2314-6133 2314-6141 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_8367585 |
| source | Wiley Online Library Open Access; PubMed Central; Alma/SFX Local Collection; PubMed Central Open Access |
| subjects | Adverse and side effects Antioxidants Apoptosis Bone marrow Bone marrow cells Cancer Cancer therapies Care and treatment Cell cycle Cell division Cellular therapy Chemical properties Chemotherapy Complications and side effects Connective tissue cells Cytokines Doxorubicin Drugs Enzymes Flow cytometry Follicle-stimulating hormone Gonads Growth factors Health aspects Hematopoietic stem cells Infertility Intoxication Laboratory animals Lipid peroxidation Luteinizing hormone Males Mesenchyme Oxidative stress Placenta Sperm Spermatogenesis Stem cell transplantation Stem cells Stromal cells Testes Testosterone Therapeutics, Experimental Toxicity Transplantation Tubules Tumors |
| title | Therapeutic Effect of Murine Bone Marrow-Derived Mesenchymal Stromal/Stem Cells and Human Placental Extract on Testicular Toxicity Resulting from Doxorubicin in Rats |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-31T00%3A00%3A27IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Therapeutic%20Effect%20of%20Murine%20Bone%20Marrow-Derived%20Mesenchymal%20Stromal/Stem%20Cells%20and%20Human%20Placental%20Extract%20on%20Testicular%20Toxicity%20Resulting%20from%20Doxorubicin%20in%20Rats&rft.jtitle=BioMed%20research%20international&rft.au=AbdRabou,%20Mervat%20Ahmed&rft.date=2021&rft.volume=2021&rft.issue=1&rft.spage=9979670&rft.epage=9979670&rft.pages=9979670-9979670&rft.issn=2314-6133&rft.eissn=2314-6141&rft_id=info:doi/10.1155/2021/9979670&rft_dat=%3Cgale_pubme%3EA696874503%3C/gale_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2563363823&rft_id=info:pmid/34409109&rft_galeid=A696874503&rfr_iscdi=true |