Nocathioamides, Uncovered by a Tunable Metabologenomic Approach, Define a Novel Class of Chimeric Lanthipeptides
The increasing number of available genomes, in combination with advanced genome mining techniques, unveiled a plethora of biosynthetic gene clusters (BGCs) coding for ribosomally synthesized and post‐translationally modified peptides (RiPPs). The products of these BGCs often represent an enormous re...
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| Veröffentlicht in: | Angewandte Chemie International Edition 2021-07, Vol.60 (30), p.16472-16479 |
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| creator | Saad, Hamada Aziz, Saefuddin Gehringer, Matthias Kramer, Markus Straetener, Jan Berscheid, Anne Brötz‐Oesterhelt, Heike Gross, Harald |
| description | The increasing number of available genomes, in combination with advanced genome mining techniques, unveiled a plethora of biosynthetic gene clusters (BGCs) coding for ribosomally synthesized and post‐translationally modified peptides (RiPPs). The products of these BGCs often represent an enormous resource for new and bioactive compounds, but frequently, they cannot be readily isolated and remain cryptic. Here, we describe a tunable metabologenomic approach that recruits a synergism of bioinformatics in tandem with isotope‐ and NMR‐guided platform to identify the product of an orphan RiPP gene cluster in the genomes of Nocardia terpenica IFM 0406 and 0706T. The application of this tactic resulted in the discovery of nocathioamides family as a founder of a new class of chimeric lanthipeptides I.
Chimeric Lanthipeptides: Employing a tunable metabologenomic approach, nocathioamides A–C were isolated and elucidated. They inaugurate a new RiPP family, characterized by a unique and hyper degree of modifications, including an intramolecular imide bridge. |
| doi_str_mv | 10.1002/anie.202102571 |
| format | Article |
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Chimeric Lanthipeptides: Employing a tunable metabologenomic approach, nocathioamides A–C were isolated and elucidated. They inaugurate a new RiPP family, characterized by a unique and hyper degree of modifications, including an intramolecular imide bridge.</description><subject>Bioactive compounds</subject><subject>Bioinformatics</subject><subject>chimeric lanthipeptide</subject><subject>Gene clusters</subject><subject>Genomes</subject><subject>macrocyclic imide</subject><subject>metabologenomic approach</subject><subject>NMR</subject><subject>Nocardia terpenica</subject><subject>Nuclear magnetic resonance</subject><subject>Peptides</subject><subject>RiPPs</subject><subject>Synergism</subject><issn>1433-7851</issn><issn>1521-3773</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>24P</sourceid><sourceid>WIN</sourceid><recordid>eNqFkU1v1DAQhiMEoqVw5YgsceHQLP5YJ_YFabUUqLQsl_ZsTZxJ11ViBzsp2n-PV1uWjwsn2_Izz8zoLYrXjC4Ypfw9eIcLTjmjXNbsSXHOJGelqGvxNN-XQpS1kuyseJHSfeaVotXz4kwIrRkV-rwYt8HCtHMBBtdiuiS33oYHjNiSZk-A3Mwemh7JV5ygCX24Qx8GZ8lqHGMAu7skH7FzHjO6zXU9WfeQEgkdWe_cgDGjG_C5wYjjdOjwsnjWQZ_w1eN5Udx-urpZfyk33z5fr1eb0kqxZGVjgWva1o3UsqIUOt0qiVaCyM-uVYrZDrFudFUx7Ja1qJRtODag2qWGthMXxYejd5ybAVuLforQmzG6AeLeBHDm7x_vduYuPBglKs50lQXvHgUxfJ8xTWZwyWLfg8cwJ8MlV6xWrJIZffsPeh_m6PN6mZI5HC7EgVocKRtDShG70zCMmkOY5hCmOYWZC978ucIJ_5VeBvQR-OF63P9HZ1bb66vf8p8c1q1p</recordid><startdate>20210719</startdate><enddate>20210719</enddate><creator>Saad, Hamada</creator><creator>Aziz, Saefuddin</creator><creator>Gehringer, Matthias</creator><creator>Kramer, Markus</creator><creator>Straetener, Jan</creator><creator>Berscheid, Anne</creator><creator>Brötz‐Oesterhelt, Heike</creator><creator>Gross, Harald</creator><general>Wiley Subscription Services, Inc</general><general>John Wiley and Sons Inc</general><scope>24P</scope><scope>WIN</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>K9.</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-0731-821X</orcidid></search><sort><creationdate>20210719</creationdate><title>Nocathioamides, Uncovered by a Tunable Metabologenomic Approach, Define a Novel Class of Chimeric Lanthipeptides</title><author>Saad, Hamada ; Aziz, Saefuddin ; Gehringer, Matthias ; Kramer, Markus ; Straetener, Jan ; Berscheid, Anne ; Brötz‐Oesterhelt, Heike ; Gross, Harald</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5341-bca290d7b595600af9d85ec5a3560fd881cfee7b9661ef47368cb2eba8d49adf3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Bioactive compounds</topic><topic>Bioinformatics</topic><topic>chimeric lanthipeptide</topic><topic>Gene clusters</topic><topic>Genomes</topic><topic>macrocyclic imide</topic><topic>metabologenomic approach</topic><topic>NMR</topic><topic>Nocardia terpenica</topic><topic>Nuclear magnetic resonance</topic><topic>Peptides</topic><topic>RiPPs</topic><topic>Synergism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Saad, Hamada</creatorcontrib><creatorcontrib>Aziz, Saefuddin</creatorcontrib><creatorcontrib>Gehringer, Matthias</creatorcontrib><creatorcontrib>Kramer, Markus</creatorcontrib><creatorcontrib>Straetener, Jan</creatorcontrib><creatorcontrib>Berscheid, Anne</creatorcontrib><creatorcontrib>Brötz‐Oesterhelt, Heike</creatorcontrib><creatorcontrib>Gross, Harald</creatorcontrib><collection>Wiley-Blackwell Open Access Titles</collection><collection>Wiley Free Content</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Angewandte Chemie International Edition</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Saad, Hamada</au><au>Aziz, Saefuddin</au><au>Gehringer, Matthias</au><au>Kramer, Markus</au><au>Straetener, Jan</au><au>Berscheid, Anne</au><au>Brötz‐Oesterhelt, Heike</au><au>Gross, Harald</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Nocathioamides, Uncovered by a Tunable Metabologenomic Approach, Define a Novel Class of Chimeric Lanthipeptides</atitle><jtitle>Angewandte Chemie International Edition</jtitle><addtitle>Angew Chem Int Ed Engl</addtitle><date>2021-07-19</date><risdate>2021</risdate><volume>60</volume><issue>30</issue><spage>16472</spage><epage>16479</epage><pages>16472-16479</pages><issn>1433-7851</issn><eissn>1521-3773</eissn><abstract>The increasing number of available genomes, in combination with advanced genome mining techniques, unveiled a plethora of biosynthetic gene clusters (BGCs) coding for ribosomally synthesized and post‐translationally modified peptides (RiPPs). 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| subjects | Bioactive compounds Bioinformatics chimeric lanthipeptide Gene clusters Genomes macrocyclic imide metabologenomic approach NMR Nocardia terpenica Nuclear magnetic resonance Peptides RiPPs Synergism |
| title | Nocathioamides, Uncovered by a Tunable Metabologenomic Approach, Define a Novel Class of Chimeric Lanthipeptides |
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