Bi-paratopic and multivalent VH domains block ACE2 binding and neutralize SARS-CoV-2

Neutralizing agents against SARS-CoV-2 are urgently needed for the treatment and prophylaxis of COVID-19. Here, we present a strategy to rapidly identify and assemble synthetic human variable heavy (VH) domains toward neutralizing epitopes. We constructed a VH-phage library and targeted the angioten...

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Veröffentlicht in:	Nature chemical biology 2021-01, Vol.17 (1), p.113-121
Hauptverfasser:	Bracken, Colton J., Lim, Shion A., Solomon, Paige, Rettko, Nicholas J., Nguyen, Duy P., Zha, Beth Shoshana, Schaefer, Kaitlin, Byrnes, James R., Zhou, Jie, Lui, Irene, Liu, Jia, Pance, Katarina, Zhou, Xin X., Leung, Kevin K., Wells, James A.
Format:	Artikel
Sprache:	eng
Schlagworte:	631/154/51 631/250/255 631/326/596/4130 631/45/535 631/92/469 ACE2 Affinity Angiotensin Angiotensin-converting enzyme 2 Angiotensin-Converting Enzyme 2 - antagonists & inhibitors Angiotensin-Converting Enzyme 2 - chemistry Angiotensin-Converting Enzyme 2 - genetics Angiotensin-Converting Enzyme 2 - immunology Animals Antibodies Antibodies, Neutralizing - chemistry Antibodies, Neutralizing - genetics Antibodies, Neutralizing - immunology Antibodies, Viral - chemistry Antibodies, Viral - genetics Antibodies, Viral - immunology Binders Binding sites Binding Sites, Antibody - genetics Binding Sites, Antibody - immunology Biochemical Engineering Biochemistry Bioorganic Chemistry Cell Biology Chemistry Chemistry and Materials Science Chemistry/Food Science Chlorocebus aethiops COVID-19 Cryoelectron Microscopy Epitopes HEK293 Cells Humans Models, Molecular Neutralization Neutralizing Peptide Library Peptidyl-dipeptidase A Phages Prophylaxis Protein Binding Protein Conformation, alpha-Helical Protein Conformation, beta-Strand Protein Interaction Domains and Motifs Receptors SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 Single-Chain Antibodies - chemistry Single-Chain Antibodies - genetics Single-Chain Antibodies - immunology Spike Glycoprotein, Coronavirus - antagonists & inhibitors Spike Glycoprotein, Coronavirus - chemistry Spike Glycoprotein, Coronavirus - genetics Spike Glycoprotein, Coronavirus - immunology Spike protein Vero Cells
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