The Early Diagnostic Efficacy of Serum Histone H3 in Rabbit Urosepsis Model
Objective. We want to explore the changing law of circulating histones in the acute stage of urosepsis and to find more sensitive and specific biomarkers for diagnosing urosepsis as early as possible. Methods. Twenty healthy male New Zealand rabbits were randomly divided into 4 groups (N=5): the con...
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| description | Objective. We want to explore the changing law of circulating histones in the acute stage of urosepsis and to find more sensitive and specific biomarkers for diagnosing urosepsis as early as possible. Methods. Twenty healthy male New Zealand rabbits were randomly divided into 4 groups (N=5): the control group, sham group, model group of LPS 600 μg/kg, and model group of LPS 1000 μg/kg. Heart rate (HR), respiration rate (RR), rectal temperature (T), and mean arterial pressure (MAP) were examined at 1, 3, 6, 12, and 24 hours after operation. Besides, peripheral blood cell counts (RBC, WBC, PLT, and Hb) and C reaction protein (CRP) were tested at 1, 3, and 6 hours after operation, while the levels of histone H3, MMP-9, TIMP-1, and procalcitonin (PCT) in the serum were tested at 1, 3, and 6 hours after operation by ELISA. The heart, left lung, liver, and left kidney were harvested for HE stain and observed to research the pathological change of these tissues. Results. (1) The general status of rabbits: rabbits in the control and sham groups came out in 2 h after operation and regain to drink and eat in 12-24 h after operation. State of the rabbits in the control group was better than that in the sham group. Rabbits in the model groups were languid after operation and stopped to drink and eat. (2) Vital signs of rabbits: there was no statistic difference in HR (P=0.238) and RR (P=0.813) among all groups. MAP of the model groups decreased at 3 h postoperative, but transient (P |
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| fullrecord | <record><control><sourceid>gale_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_8310443</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A696881673</galeid><sourcerecordid>A696881673</sourcerecordid><originalsourceid>FETCH-LOGICAL-c570t-bacb90e70ef2eb966ef3ca0ff030f1ea4156a52605c3e130d4ec3758e95de4983</originalsourceid><addsrcrecordid>eNp9kc1rFDEYh4MottTePEvAi6Br8z2Ti1Dq6ooVQdtzyGTe7KbMJttkRtn_3iy7rh-H5pLA-_CE9_dD6DklbymV8oIRRi-0VpoL8QidMk7FTFFBHx_fnJ-g81LuSD0tVUSrp-iEC84aJtgp-nyzAjy3edji98EuYypjcHjufXDWbXHy-DvkaY0XoYwpAl5wHCL-ZrsujPg2pwKbEgr-knoYnqEn3g4Fzg_3Gbr9ML-5Wsyuv378dHV5PXOyIeOss67TBBoCnkGnlQLPnSXeE048BSuoVFYyRaTjQDnpBTjeyBa07EHolp-hd3vvZurW0DuIY7aD2eSwtnlrkg3m30kMK7NMP0zLKRGCV8GrgyCn-wnKaNahOBgGGyFNxTApG8a4IE1FX_6H3qUpx7rejhKtFpqqP9TSDmBC9Kn-63ZSc6m0amvwDX-YarlsiWayUm_2lKvplgz-uBglZle62ZVuDqVX_MXfYRzh3xVX4PUeWIXY25_hYd0vBeawWg</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2554894916</pqid></control><display><type>article</type><title>The Early Diagnostic Efficacy of Serum Histone H3 in Rabbit Urosepsis Model</title><source>MEDLINE</source><source>PubMed Central Open Access</source><source>Wiley Online Library Open Access</source><source>PubMed Central</source><source>Alma/SFX Local Collection</source><creator>Zhang, Xiaolu ; Zhan, Xiangcheng ; Huang, Bisheng ; Li, Saiyang ; Xu, Yunfei</creator><contributor>Huang, Tao ; Tao Huang</contributor><creatorcontrib>Zhang, Xiaolu ; Zhan, Xiangcheng ; Huang, Bisheng ; Li, Saiyang ; Xu, Yunfei ; Huang, Tao ; Tao Huang</creatorcontrib><description>Objective. We want to explore the changing law of circulating histones in the acute stage of urosepsis and to find more sensitive and specific biomarkers for diagnosing urosepsis as early as possible. Methods. Twenty healthy male New Zealand rabbits were randomly divided into 4 groups (N=5): the control group, sham group, model group of LPS 600 μg/kg, and model group of LPS 1000 μg/kg. Heart rate (HR), respiration rate (RR), rectal temperature (T), and mean arterial pressure (MAP) were examined at 1, 3, 6, 12, and 24 hours after operation. Besides, peripheral blood cell counts (RBC, WBC, PLT, and Hb) and C reaction protein (CRP) were tested at 1, 3, and 6 hours after operation, while the levels of histone H3, MMP-9, TIMP-1, and procalcitonin (PCT) in the serum were tested at 1, 3, and 6 hours after operation by ELISA. The heart, left lung, liver, and left kidney were harvested for HE stain and observed to research the pathological change of these tissues. Results. (1) The general status of rabbits: rabbits in the control and sham groups came out in 2 h after operation and regain to drink and eat in 12-24 h after operation. State of the rabbits in the control group was better than that in the sham group. Rabbits in the model groups were languid after operation and stopped to drink and eat. (2) Vital signs of rabbits: there was no statistic difference in HR (P=0.238) and RR (P=0.813) among all groups. MAP of the model groups decreased at 3 h postoperative, but transient (P<0.001). The T of the LPS 1000 group decreased at 6 h postoperative (P=0.003). (3) The change of biomarkers: H3 level of the LPS groups in the serum increased at 1 h postoperative (P<0.01); MMP-9 of the LPS 1000 group increased at 1 h postoperative (P<0.01); WBC of the model groups decreased at 3 h postoperative (P<0.05); PLT of the LPS 1000 group is significantly increased at 1 h postoperative (P<0.05); no statistic difference was found in CRP, PCT, and TIMP-1 among all groups. (4) Pathological sections: no abnormal performance was found in the control and sham groups. Glomerulus of the model groups was out of shape and necrosis with obvious renal tubule expansion. Pulmonary pathology showed alveolar septum diffuse increased and inflammatory infiltrate. Change of the LPS 1000 group was more serious than that of the LPS 600 group. Conclusions. Ligating the ureter after an injection of 1000 μg/kg LPS into the ureter of the rabbit can establish the animal model of urosepsis. Histone H3 increase immediately at 1 h postoperative and are promised to be biomarkers of urosepsis, which are more effective than WBC, CRP, and PCT.</description><identifier>ISSN: 2314-6133</identifier><identifier>EISSN: 2314-6141</identifier><identifier>DOI: 10.1155/2021/9969344</identifier><identifier>PMID: 34327242</identifier><language>eng</language><publisher>United States: Hindawi</publisher><subject>Alveoli ; Analysis ; Animal models ; Animals ; Antibiotics ; Arterial Pressure ; Biomarkers ; Blood cells ; Blood pressure ; Body Temperature ; C-Reactive Protein - metabolism ; Calculi, Urinary ; Care and treatment ; Causes of ; Complications and side effects ; Diagnosis ; Disease Models, Animal ; DNA binding proteins ; Early Diagnosis ; Enzyme-linked immunosorbent assay ; Enzymes ; Gelatinase B ; Glomerulus ; Health aspects ; Heart rate ; Histone H3 ; Histones ; Histones - blood ; Infections ; Inflammation ; Kidneys ; Laboratories ; Leukocyte Count ; Lipopolysaccharides ; Male ; Matrix Metalloproteinase 9 - metabolism ; Measurement ; Medical prognosis ; Methods ; Necrosis ; Organ Specificity ; Peripheral blood ; Platelet Count ; Procalcitonin ; Procalcitonin - blood ; Rabbits ; Risk factors ; ROC Curve ; Sensitivity and Specificity ; Sepsis ; Sepsis - blood ; Sepsis - diagnosis ; Sepsis - pathology ; Sepsis - physiopathology ; Septum ; Surgery ; Tissue inhibitor of metalloproteinase 1 ; Tissue Inhibitor of Metalloproteinase-1 - blood ; Tumor necrosis factor-TNF ; Ureter ; Urinary tract infections ; Urogenital system ; Urology ; Vital Signs</subject><ispartof>BioMed research international, 2021, Vol.2021 (1), p.9969344-9969344</ispartof><rights>Copyright © 2021 Xiaolu Zhang et al.</rights><rights>COPYRIGHT 2021 John Wiley & Sons, Inc.</rights><rights>Copyright © 2021 Xiaolu Zhang et al. This is an open access article distributed under the Creative Commons Attribution License (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. https://creativecommons.org/licenses/by/4.0</rights><rights>Copyright © 2021 Xiaolu Zhang et al. 2021</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c570t-bacb90e70ef2eb966ef3ca0ff030f1ea4156a52605c3e130d4ec3758e95de4983</citedby><cites>FETCH-LOGICAL-c570t-bacb90e70ef2eb966ef3ca0ff030f1ea4156a52605c3e130d4ec3758e95de4983</cites><orcidid>0000-0003-1288-8361</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8310443/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8310443/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,4022,27922,27923,27924,53790,53792</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34327242$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Huang, Tao</contributor><contributor>Tao Huang</contributor><creatorcontrib>Zhang, Xiaolu</creatorcontrib><creatorcontrib>Zhan, Xiangcheng</creatorcontrib><creatorcontrib>Huang, Bisheng</creatorcontrib><creatorcontrib>Li, Saiyang</creatorcontrib><creatorcontrib>Xu, Yunfei</creatorcontrib><title>The Early Diagnostic Efficacy of Serum Histone H3 in Rabbit Urosepsis Model</title><title>BioMed research international</title><addtitle>Biomed Res Int</addtitle><description>Objective. We want to explore the changing law of circulating histones in the acute stage of urosepsis and to find more sensitive and specific biomarkers for diagnosing urosepsis as early as possible. Methods. Twenty healthy male New Zealand rabbits were randomly divided into 4 groups (N=5): the control group, sham group, model group of LPS 600 μg/kg, and model group of LPS 1000 μg/kg. Heart rate (HR), respiration rate (RR), rectal temperature (T), and mean arterial pressure (MAP) were examined at 1, 3, 6, 12, and 24 hours after operation. Besides, peripheral blood cell counts (RBC, WBC, PLT, and Hb) and C reaction protein (CRP) were tested at 1, 3, and 6 hours after operation, while the levels of histone H3, MMP-9, TIMP-1, and procalcitonin (PCT) in the serum were tested at 1, 3, and 6 hours after operation by ELISA. The heart, left lung, liver, and left kidney were harvested for HE stain and observed to research the pathological change of these tissues. Results. (1) The general status of rabbits: rabbits in the control and sham groups came out in 2 h after operation and regain to drink and eat in 12-24 h after operation. State of the rabbits in the control group was better than that in the sham group. Rabbits in the model groups were languid after operation and stopped to drink and eat. (2) Vital signs of rabbits: there was no statistic difference in HR (P=0.238) and RR (P=0.813) among all groups. MAP of the model groups decreased at 3 h postoperative, but transient (P<0.001). The T of the LPS 1000 group decreased at 6 h postoperative (P=0.003). (3) The change of biomarkers: H3 level of the LPS groups in the serum increased at 1 h postoperative (P<0.01); MMP-9 of the LPS 1000 group increased at 1 h postoperative (P<0.01); WBC of the model groups decreased at 3 h postoperative (P<0.05); PLT of the LPS 1000 group is significantly increased at 1 h postoperative (P<0.05); no statistic difference was found in CRP, PCT, and TIMP-1 among all groups. (4) Pathological sections: no abnormal performance was found in the control and sham groups. Glomerulus of the model groups was out of shape and necrosis with obvious renal tubule expansion. Pulmonary pathology showed alveolar septum diffuse increased and inflammatory infiltrate. Change of the LPS 1000 group was more serious than that of the LPS 600 group. Conclusions. Ligating the ureter after an injection of 1000 μg/kg LPS into the ureter of the rabbit can establish the animal model of urosepsis. Histone H3 increase immediately at 1 h postoperative and are promised to be biomarkers of urosepsis, which are more effective than WBC, CRP, and PCT.</description><subject>Alveoli</subject><subject>Analysis</subject><subject>Animal models</subject><subject>Animals</subject><subject>Antibiotics</subject><subject>Arterial Pressure</subject><subject>Biomarkers</subject><subject>Blood cells</subject><subject>Blood pressure</subject><subject>Body Temperature</subject><subject>C-Reactive Protein - metabolism</subject><subject>Calculi, Urinary</subject><subject>Care and treatment</subject><subject>Causes of</subject><subject>Complications and side effects</subject><subject>Diagnosis</subject><subject>Disease Models, Animal</subject><subject>DNA binding proteins</subject><subject>Early Diagnosis</subject><subject>Enzyme-linked immunosorbent assay</subject><subject>Enzymes</subject><subject>Gelatinase B</subject><subject>Glomerulus</subject><subject>Health aspects</subject><subject>Heart rate</subject><subject>Histone H3</subject><subject>Histones</subject><subject>Histones - blood</subject><subject>Infections</subject><subject>Inflammation</subject><subject>Kidneys</subject><subject>Laboratories</subject><subject>Leukocyte Count</subject><subject>Lipopolysaccharides</subject><subject>Male</subject><subject>Matrix Metalloproteinase 9 - metabolism</subject><subject>Measurement</subject><subject>Medical prognosis</subject><subject>Methods</subject><subject>Necrosis</subject><subject>Organ Specificity</subject><subject>Peripheral blood</subject><subject>Platelet Count</subject><subject>Procalcitonin</subject><subject>Procalcitonin - blood</subject><subject>Rabbits</subject><subject>Risk factors</subject><subject>ROC Curve</subject><subject>Sensitivity and Specificity</subject><subject>Sepsis</subject><subject>Sepsis - blood</subject><subject>Sepsis - diagnosis</subject><subject>Sepsis - pathology</subject><subject>Sepsis - physiopathology</subject><subject>Septum</subject><subject>Surgery</subject><subject>Tissue inhibitor of metalloproteinase 1</subject><subject>Tissue Inhibitor of Metalloproteinase-1 - blood</subject><subject>Tumor necrosis factor-TNF</subject><subject>Ureter</subject><subject>Urinary tract infections</subject><subject>Urogenital system</subject><subject>Urology</subject><subject>Vital Signs</subject><issn>2314-6133</issn><issn>2314-6141</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>RHX</sourceid><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kc1rFDEYh4MottTePEvAi6Br8z2Ti1Dq6ooVQdtzyGTe7KbMJttkRtn_3iy7rh-H5pLA-_CE9_dD6DklbymV8oIRRi-0VpoL8QidMk7FTFFBHx_fnJ-g81LuSD0tVUSrp-iEC84aJtgp-nyzAjy3edji98EuYypjcHjufXDWbXHy-DvkaY0XoYwpAl5wHCL-ZrsujPg2pwKbEgr-knoYnqEn3g4Fzg_3Gbr9ML-5Wsyuv378dHV5PXOyIeOss67TBBoCnkGnlQLPnSXeE048BSuoVFYyRaTjQDnpBTjeyBa07EHolp-hd3vvZurW0DuIY7aD2eSwtnlrkg3m30kMK7NMP0zLKRGCV8GrgyCn-wnKaNahOBgGGyFNxTApG8a4IE1FX_6H3qUpx7rejhKtFpqqP9TSDmBC9Kn-63ZSc6m0amvwDX-YarlsiWayUm_2lKvplgz-uBglZle62ZVuDqVX_MXfYRzh3xVX4PUeWIXY25_hYd0vBeawWg</recordid><startdate>2021</startdate><enddate>2021</enddate><creator>Zhang, Xiaolu</creator><creator>Zhan, Xiangcheng</creator><creator>Huang, Bisheng</creator><creator>Li, Saiyang</creator><creator>Xu, Yunfei</creator><general>Hindawi</general><general>John Wiley & Sons, Inc</general><general>Hindawi Limited</general><scope>RHU</scope><scope>RHW</scope><scope>RHX</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7QO</scope><scope>7T7</scope><scope>7TK</scope><scope>7U7</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>CWDGH</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0003-1288-8361</orcidid></search><sort><creationdate>2021</creationdate><title>The Early Diagnostic Efficacy of Serum Histone H3 in Rabbit Urosepsis Model</title><author>Zhang, Xiaolu ; Zhan, Xiangcheng ; Huang, Bisheng ; Li, Saiyang ; Xu, Yunfei</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c570t-bacb90e70ef2eb966ef3ca0ff030f1ea4156a52605c3e130d4ec3758e95de4983</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Alveoli</topic><topic>Analysis</topic><topic>Animal models</topic><topic>Animals</topic><topic>Antibiotics</topic><topic>Arterial Pressure</topic><topic>Biomarkers</topic><topic>Blood cells</topic><topic>Blood pressure</topic><topic>Body Temperature</topic><topic>C-Reactive Protein - metabolism</topic><topic>Calculi, Urinary</topic><topic>Care and treatment</topic><topic>Causes of</topic><topic>Complications and side effects</topic><topic>Diagnosis</topic><topic>Disease Models, Animal</topic><topic>DNA binding proteins</topic><topic>Early Diagnosis</topic><topic>Enzyme-linked immunosorbent assay</topic><topic>Enzymes</topic><topic>Gelatinase B</topic><topic>Glomerulus</topic><topic>Health aspects</topic><topic>Heart rate</topic><topic>Histone H3</topic><topic>Histones</topic><topic>Histones - blood</topic><topic>Infections</topic><topic>Inflammation</topic><topic>Kidneys</topic><topic>Laboratories</topic><topic>Leukocyte Count</topic><topic>Lipopolysaccharides</topic><topic>Male</topic><topic>Matrix Metalloproteinase 9 - metabolism</topic><topic>Measurement</topic><topic>Medical prognosis</topic><topic>Methods</topic><topic>Necrosis</topic><topic>Organ Specificity</topic><topic>Peripheral blood</topic><topic>Platelet Count</topic><topic>Procalcitonin</topic><topic>Procalcitonin - blood</topic><topic>Rabbits</topic><topic>Risk factors</topic><topic>ROC Curve</topic><topic>Sensitivity and Specificity</topic><topic>Sepsis</topic><topic>Sepsis - blood</topic><topic>Sepsis - diagnosis</topic><topic>Sepsis - pathology</topic><topic>Sepsis - physiopathology</topic><topic>Septum</topic><topic>Surgery</topic><topic>Tissue inhibitor of metalloproteinase 1</topic><topic>Tissue Inhibitor of Metalloproteinase-1 - blood</topic><topic>Tumor necrosis factor-TNF</topic><topic>Ureter</topic><topic>Urinary tract infections</topic><topic>Urogenital system</topic><topic>Urology</topic><topic>Vital Signs</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhang, Xiaolu</creatorcontrib><creatorcontrib>Zhan, Xiangcheng</creatorcontrib><creatorcontrib>Huang, Bisheng</creatorcontrib><creatorcontrib>Li, Saiyang</creatorcontrib><creatorcontrib>Xu, Yunfei</creatorcontrib><collection>Hindawi Publishing Complete</collection><collection>Hindawi Publishing Subscription Journals</collection><collection>Hindawi Publishing Open Access Journals</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>Middle East & Africa Database</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>BioMed research international</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhang, Xiaolu</au><au>Zhan, Xiangcheng</au><au>Huang, Bisheng</au><au>Li, Saiyang</au><au>Xu, Yunfei</au><au>Huang, Tao</au><au>Tao Huang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The Early Diagnostic Efficacy of Serum Histone H3 in Rabbit Urosepsis Model</atitle><jtitle>BioMed research international</jtitle><addtitle>Biomed Res Int</addtitle><date>2021</date><risdate>2021</risdate><volume>2021</volume><issue>1</issue><spage>9969344</spage><epage>9969344</epage><pages>9969344-9969344</pages><issn>2314-6133</issn><eissn>2314-6141</eissn><abstract>Objective. We want to explore the changing law of circulating histones in the acute stage of urosepsis and to find more sensitive and specific biomarkers for diagnosing urosepsis as early as possible. Methods. Twenty healthy male New Zealand rabbits were randomly divided into 4 groups (N=5): the control group, sham group, model group of LPS 600 μg/kg, and model group of LPS 1000 μg/kg. Heart rate (HR), respiration rate (RR), rectal temperature (T), and mean arterial pressure (MAP) were examined at 1, 3, 6, 12, and 24 hours after operation. Besides, peripheral blood cell counts (RBC, WBC, PLT, and Hb) and C reaction protein (CRP) were tested at 1, 3, and 6 hours after operation, while the levels of histone H3, MMP-9, TIMP-1, and procalcitonin (PCT) in the serum were tested at 1, 3, and 6 hours after operation by ELISA. The heart, left lung, liver, and left kidney were harvested for HE stain and observed to research the pathological change of these tissues. Results. (1) The general status of rabbits: rabbits in the control and sham groups came out in 2 h after operation and regain to drink and eat in 12-24 h after operation. State of the rabbits in the control group was better than that in the sham group. Rabbits in the model groups were languid after operation and stopped to drink and eat. (2) Vital signs of rabbits: there was no statistic difference in HR (P=0.238) and RR (P=0.813) among all groups. MAP of the model groups decreased at 3 h postoperative, but transient (P<0.001). The T of the LPS 1000 group decreased at 6 h postoperative (P=0.003). (3) The change of biomarkers: H3 level of the LPS groups in the serum increased at 1 h postoperative (P<0.01); MMP-9 of the LPS 1000 group increased at 1 h postoperative (P<0.01); WBC of the model groups decreased at 3 h postoperative (P<0.05); PLT of the LPS 1000 group is significantly increased at 1 h postoperative (P<0.05); no statistic difference was found in CRP, PCT, and TIMP-1 among all groups. (4) Pathological sections: no abnormal performance was found in the control and sham groups. Glomerulus of the model groups was out of shape and necrosis with obvious renal tubule expansion. Pulmonary pathology showed alveolar septum diffuse increased and inflammatory infiltrate. Change of the LPS 1000 group was more serious than that of the LPS 600 group. Conclusions. Ligating the ureter after an injection of 1000 μg/kg LPS into the ureter of the rabbit can establish the animal model of urosepsis. Histone H3 increase immediately at 1 h postoperative and are promised to be biomarkers of urosepsis, which are more effective than WBC, CRP, and PCT.</abstract><cop>United States</cop><pub>Hindawi</pub><pmid>34327242</pmid><doi>10.1155/2021/9969344</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0003-1288-8361</orcidid><oa>free_for_read</oa></addata></record> |
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| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_8310443 |
| source | MEDLINE; PubMed Central Open Access; Wiley Online Library Open Access; PubMed Central; Alma/SFX Local Collection |
| subjects | Alveoli Analysis Animal models Animals Antibiotics Arterial Pressure Biomarkers Blood cells Blood pressure Body Temperature C-Reactive Protein - metabolism Calculi, Urinary Care and treatment Causes of Complications and side effects Diagnosis Disease Models, Animal DNA binding proteins Early Diagnosis Enzyme-linked immunosorbent assay Enzymes Gelatinase B Glomerulus Health aspects Heart rate Histone H3 Histones Histones - blood Infections Inflammation Kidneys Laboratories Leukocyte Count Lipopolysaccharides Male Matrix Metalloproteinase 9 - metabolism Measurement Medical prognosis Methods Necrosis Organ Specificity Peripheral blood Platelet Count Procalcitonin Procalcitonin - blood Rabbits Risk factors ROC Curve Sensitivity and Specificity Sepsis Sepsis - blood Sepsis - diagnosis Sepsis - pathology Sepsis - physiopathology Septum Surgery Tissue inhibitor of metalloproteinase 1 Tissue Inhibitor of Metalloproteinase-1 - blood Tumor necrosis factor-TNF Ureter Urinary tract infections Urogenital system Urology Vital Signs |
| title | The Early Diagnostic Efficacy of Serum Histone H3 in Rabbit Urosepsis Model |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-10T10%3A54%3A11IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=The%20Early%20Diagnostic%20Efficacy%20of%20Serum%20Histone%20H3%20in%20Rabbit%20Urosepsis%20Model&rft.jtitle=BioMed%20research%20international&rft.au=Zhang,%20Xiaolu&rft.date=2021&rft.volume=2021&rft.issue=1&rft.spage=9969344&rft.epage=9969344&rft.pages=9969344-9969344&rft.issn=2314-6133&rft.eissn=2314-6141&rft_id=info:doi/10.1155/2021/9969344&rft_dat=%3Cgale_pubme%3EA696881673%3C/gale_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2554894916&rft_id=info:pmid/34327242&rft_galeid=A696881673&rfr_iscdi=true |