Physiological Changes and Differential Gene Expression of Tea Plants ( Camellia sinensis (L.) Kuntze var. niaowangensis Q.H. Chen) Under Cold Stress

Low temperature is an important factor that affects the growth and reproduction of tea plants [Camellia sinensis (L.) Kuntze]. In this study, Yunwu Tribute Tea cutting seedlings [Camellia sinensis (L.) Kuntze var. niaowangensis Q.H. Chen] were subjected to different low-temperature treatments in Gui...

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Veröffentlicht in:DNA and cell biology 2021-07, Vol.40 (7), p.906-920
Hauptverfasser: Wang, Ying, Li, Yan, Wang, Jihong, Xiang, Zhun, Xi, Peiyu, Zhao, Degang
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container_issue 7
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creator Wang, Ying
Li, Yan
Wang, Jihong
Xiang, Zhun
Xi, Peiyu
Zhao, Degang
description Low temperature is an important factor that affects the growth and reproduction of tea plants [Camellia sinensis (L.) Kuntze]. In this study, Yunwu Tribute Tea cutting seedlings [Camellia sinensis (L.) Kuntze var. niaowangensis Q.H. Chen] were subjected to different low-temperature treatments in Guizhou Province, China, and the changes in physiological indicators of the leaves were measured to investigate the physiological response and cold tolerance of this variety. Under cold stress, the peak of antioxidant enzyme activity appeared on the third day of treatment at 1°C, indicating that Yunwu Tribute Tea could improve the resistance to cold stress through an increase in enzyme activity within a low-temperature range. However, after 3 days treatment at 1°C, the tolerance of plant had been exceeded; the ability to resist cold stress disappeared, and enzyme activity decreased. When the temperature or duration of stress exceeded the maximum tolerance of the plant, the synthesis of soluble substances decreased in concert with their protective effects. Under cold conditions, Yunwu Tribute Tea could increase the production of abscisic acid growth inhibitors and reduce those of indoleacetic acid, gibberellin, and other growth promoting substances to manage cold stress by regulating the balance of growth regulators in the plant. Five differential genes were screened as candidate genes from the Yunwu Tribute Tea cold stress transcriptome (DW, 1°C) for fluorescence quantitative analysis. The results showed that the changes in levels of expression of these genes under continuous cold stress significantly positively correlated with the corresponding physiological indicators. Nevertheless, the levels of expression of the Yunwu Tribute Tea polyphenol oxidase (PPO) gene and the gibberellin 3β-dioxygenase gene (G3O2) were reversely inhibited under cold stress. The result was consistent with the corresponding physiological indicators, and it provides a basis for the study of cold resistance mechanisms in tea plants.
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Kuntze var. niaowangensis Q.H. Chen) Under Cold Stress</title><source>Alma/SFX Local Collection</source><creator>Wang, Ying ; Li, Yan ; Wang, Jihong ; Xiang, Zhun ; Xi, Peiyu ; Zhao, Degang</creator><creatorcontrib>Wang, Ying ; Li, Yan ; Wang, Jihong ; Xiang, Zhun ; Xi, Peiyu ; Zhao, Degang</creatorcontrib><description>Low temperature is an important factor that affects the growth and reproduction of tea plants [Camellia sinensis (L.) Kuntze]. In this study, Yunwu Tribute Tea cutting seedlings [Camellia sinensis (L.) Kuntze var. niaowangensis Q.H. Chen] were subjected to different low-temperature treatments in Guizhou Province, China, and the changes in physiological indicators of the leaves were measured to investigate the physiological response and cold tolerance of this variety. Under cold stress, the peak of antioxidant enzyme activity appeared on the third day of treatment at 1°C, indicating that Yunwu Tribute Tea could improve the resistance to cold stress through an increase in enzyme activity within a low-temperature range. However, after 3 days treatment at 1°C, the tolerance of plant had been exceeded; the ability to resist cold stress disappeared, and enzyme activity decreased. When the temperature or duration of stress exceeded the maximum tolerance of the plant, the synthesis of soluble substances decreased in concert with their protective effects. Under cold conditions, Yunwu Tribute Tea could increase the production of abscisic acid growth inhibitors and reduce those of indoleacetic acid, gibberellin, and other growth promoting substances to manage cold stress by regulating the balance of growth regulators in the plant. Five differential genes were screened as candidate genes from the Yunwu Tribute Tea cold stress transcriptome (DW, 1°C) for fluorescence quantitative analysis. The results showed that the changes in levels of expression of these genes under continuous cold stress significantly positively correlated with the corresponding physiological indicators. Nevertheless, the levels of expression of the Yunwu Tribute Tea polyphenol oxidase (PPO) gene and the gibberellin 3β-dioxygenase gene (G3O2) were reversely inhibited under cold stress. The result was consistent with the corresponding physiological indicators, and it provides a basis for the study of cold resistance mechanisms in tea plants.</description><identifier>ISSN: 1044-5498</identifier><identifier>EISSN: 1557-7430</identifier><identifier>DOI: 10.1089/dna.2021.0147</identifier><identifier>PMID: 34129383</identifier><language>eng</language><publisher>New Rochelle: Mary Ann Liebert, Inc</publisher><subject>Abscisic acid ; Antioxidants ; Camellia sinensis ; Cold ; Cold resistance ; Cold tolerance ; Enzymatic activity ; Enzyme activity ; Enzymes ; Fluorescence ; Gene expression ; Genes ; Gibberellin 3β-dioxygenase ; Gibberellins ; Growth regulators ; Indicators ; Indoleacetic acid ; Low temperature ; Low temperature resistance ; Molecular Genetics/Genomics/Epigenetics ; Physiology ; Polyphenol oxidase ; Seedlings ; Stress ; Tea ; Temperature tolerance ; Transcriptomes</subject><ispartof>DNA and cell biology, 2021-07, Vol.40 (7), p.906-920</ispartof><rights>Copyright Mary Ann Liebert, Inc. 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Kuntze var. niaowangensis Q.H. Chen) Under Cold Stress</title><title>DNA and cell biology</title><description>Low temperature is an important factor that affects the growth and reproduction of tea plants [Camellia sinensis (L.) Kuntze]. In this study, Yunwu Tribute Tea cutting seedlings [Camellia sinensis (L.) Kuntze var. niaowangensis Q.H. Chen] were subjected to different low-temperature treatments in Guizhou Province, China, and the changes in physiological indicators of the leaves were measured to investigate the physiological response and cold tolerance of this variety. Under cold stress, the peak of antioxidant enzyme activity appeared on the third day of treatment at 1°C, indicating that Yunwu Tribute Tea could improve the resistance to cold stress through an increase in enzyme activity within a low-temperature range. However, after 3 days treatment at 1°C, the tolerance of plant had been exceeded; the ability to resist cold stress disappeared, and enzyme activity decreased. When the temperature or duration of stress exceeded the maximum tolerance of the plant, the synthesis of soluble substances decreased in concert with their protective effects. Under cold conditions, Yunwu Tribute Tea could increase the production of abscisic acid growth inhibitors and reduce those of indoleacetic acid, gibberellin, and other growth promoting substances to manage cold stress by regulating the balance of growth regulators in the plant. Five differential genes were screened as candidate genes from the Yunwu Tribute Tea cold stress transcriptome (DW, 1°C) for fluorescence quantitative analysis. The results showed that the changes in levels of expression of these genes under continuous cold stress significantly positively correlated with the corresponding physiological indicators. Nevertheless, the levels of expression of the Yunwu Tribute Tea polyphenol oxidase (PPO) gene and the gibberellin 3β-dioxygenase gene (G3O2) were reversely inhibited under cold stress. The result was consistent with the corresponding physiological indicators, and it provides a basis for the study of cold resistance mechanisms in tea plants.</description><subject>Abscisic acid</subject><subject>Antioxidants</subject><subject>Camellia sinensis</subject><subject>Cold</subject><subject>Cold resistance</subject><subject>Cold tolerance</subject><subject>Enzymatic activity</subject><subject>Enzyme activity</subject><subject>Enzymes</subject><subject>Fluorescence</subject><subject>Gene expression</subject><subject>Genes</subject><subject>Gibberellin 3β-dioxygenase</subject><subject>Gibberellins</subject><subject>Growth regulators</subject><subject>Indicators</subject><subject>Indoleacetic acid</subject><subject>Low temperature</subject><subject>Low temperature resistance</subject><subject>Molecular Genetics/Genomics/Epigenetics</subject><subject>Physiology</subject><subject>Polyphenol oxidase</subject><subject>Seedlings</subject><subject>Stress</subject><subject>Tea</subject><subject>Temperature tolerance</subject><subject>Transcriptomes</subject><issn>1044-5498</issn><issn>1557-7430</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><recordid>eNpdkc1u1DAUhSMEoqWwZG-JTbtI8F-ceIOE0tIiRqKIdm3dxDczrjL2YGcK7XP0gXFohQQrWz6fzrnXpyjeMlox2ur31kPFKWcVZbJ5Vhyyum7KRgr6PN-plGUtdXtQvErphlJac0ZfFgdCMq5FKw6Lh8vNXXJhCms3wES6Dfg1JgLeklM3jhjRzy4L5-iRnP3aRUwZ9ySM5AqBXE7g50SOSQdbnCYHJDmPPrn8tqpOyJe9n--R3EKsiHcQfi72f-Rv1UWV49CfkGtvMZIuTJZ8n5eA18WLEaaEb57Oo-L609lVd1Guvp5_7j6uykFoPpeaauwbpkcYWo5K1AostL2ttYbGWl5bhc0ghZVUMa76lktQfJDQc9FzJcRR8eHRd7fvt2iHvGuEyeyi20K8MwGc-VfxbmPW4da0gmopdDY4fjKI4cce02y2Lg35I8Bj2CfDa8maljG9ZL37D70J--jzepmqBeVKKZmp8pEaYkgp4vh3GEbN0rfJfZulb7P0LX4DbRWctQ</recordid><startdate>20210701</startdate><enddate>20210701</enddate><creator>Wang, Ying</creator><creator>Li, Yan</creator><creator>Wang, Jihong</creator><creator>Xiang, Zhun</creator><creator>Xi, Peiyu</creator><creator>Zhao, Degang</creator><general>Mary Ann Liebert, Inc</general><general>Mary Ann Liebert, Inc., publishers</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-0294-6428</orcidid></search><sort><creationdate>20210701</creationdate><title>Physiological Changes and Differential Gene Expression of Tea Plants ( Camellia sinensis (L.) Kuntze var. niaowangensis Q.H. Chen) Under Cold Stress</title><author>Wang, Ying ; Li, Yan ; Wang, Jihong ; Xiang, Zhun ; Xi, Peiyu ; Zhao, Degang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c392t-909eb719fac82e6356ada8bd599a7dd25d6e7c43d406126b824a62c4ab23b2633</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Abscisic acid</topic><topic>Antioxidants</topic><topic>Camellia sinensis</topic><topic>Cold</topic><topic>Cold resistance</topic><topic>Cold tolerance</topic><topic>Enzymatic activity</topic><topic>Enzyme activity</topic><topic>Enzymes</topic><topic>Fluorescence</topic><topic>Gene expression</topic><topic>Genes</topic><topic>Gibberellin 3β-dioxygenase</topic><topic>Gibberellins</topic><topic>Growth regulators</topic><topic>Indicators</topic><topic>Indoleacetic acid</topic><topic>Low temperature</topic><topic>Low temperature resistance</topic><topic>Molecular Genetics/Genomics/Epigenetics</topic><topic>Physiology</topic><topic>Polyphenol oxidase</topic><topic>Seedlings</topic><topic>Stress</topic><topic>Tea</topic><topic>Temperature tolerance</topic><topic>Transcriptomes</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wang, Ying</creatorcontrib><creatorcontrib>Li, Yan</creatorcontrib><creatorcontrib>Wang, Jihong</creatorcontrib><creatorcontrib>Xiang, Zhun</creatorcontrib><creatorcontrib>Xi, Peiyu</creatorcontrib><creatorcontrib>Zhao, Degang</creatorcontrib><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium &amp; Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>DNA and cell biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, Ying</au><au>Li, Yan</au><au>Wang, Jihong</au><au>Xiang, Zhun</au><au>Xi, Peiyu</au><au>Zhao, Degang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Physiological Changes and Differential Gene Expression of Tea Plants ( Camellia sinensis (L.) Kuntze var. niaowangensis Q.H. Chen) Under Cold Stress</atitle><jtitle>DNA and cell biology</jtitle><date>2021-07-01</date><risdate>2021</risdate><volume>40</volume><issue>7</issue><spage>906</spage><epage>920</epage><pages>906-920</pages><issn>1044-5498</issn><eissn>1557-7430</eissn><abstract>Low temperature is an important factor that affects the growth and reproduction of tea plants [Camellia sinensis (L.) Kuntze]. In this study, Yunwu Tribute Tea cutting seedlings [Camellia sinensis (L.) Kuntze var. niaowangensis Q.H. Chen] were subjected to different low-temperature treatments in Guizhou Province, China, and the changes in physiological indicators of the leaves were measured to investigate the physiological response and cold tolerance of this variety. Under cold stress, the peak of antioxidant enzyme activity appeared on the third day of treatment at 1°C, indicating that Yunwu Tribute Tea could improve the resistance to cold stress through an increase in enzyme activity within a low-temperature range. However, after 3 days treatment at 1°C, the tolerance of plant had been exceeded; the ability to resist cold stress disappeared, and enzyme activity decreased. When the temperature or duration of stress exceeded the maximum tolerance of the plant, the synthesis of soluble substances decreased in concert with their protective effects. Under cold conditions, Yunwu Tribute Tea could increase the production of abscisic acid growth inhibitors and reduce those of indoleacetic acid, gibberellin, and other growth promoting substances to manage cold stress by regulating the balance of growth regulators in the plant. Five differential genes were screened as candidate genes from the Yunwu Tribute Tea cold stress transcriptome (DW, 1°C) for fluorescence quantitative analysis. The results showed that the changes in levels of expression of these genes under continuous cold stress significantly positively correlated with the corresponding physiological indicators. Nevertheless, the levels of expression of the Yunwu Tribute Tea polyphenol oxidase (PPO) gene and the gibberellin 3β-dioxygenase gene (G3O2) were reversely inhibited under cold stress. The result was consistent with the corresponding physiological indicators, and it provides a basis for the study of cold resistance mechanisms in tea plants.</abstract><cop>New Rochelle</cop><pub>Mary Ann Liebert, Inc</pub><pmid>34129383</pmid><doi>10.1089/dna.2021.0147</doi><tpages>15</tpages><orcidid>https://orcid.org/0000-0002-0294-6428</orcidid><oa>free_for_read</oa></addata></record>
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source Alma/SFX Local Collection
subjects Abscisic acid
Antioxidants
Camellia sinensis
Cold
Cold resistance
Cold tolerance
Enzymatic activity
Enzyme activity
Enzymes
Fluorescence
Gene expression
Genes
Gibberellin 3β-dioxygenase
Gibberellins
Growth regulators
Indicators
Indoleacetic acid
Low temperature
Low temperature resistance
Molecular Genetics/Genomics/Epigenetics
Physiology
Polyphenol oxidase
Seedlings
Stress
Tea
Temperature tolerance
Transcriptomes
title Physiological Changes and Differential Gene Expression of Tea Plants ( Camellia sinensis (L.) Kuntze var. niaowangensis Q.H. Chen) Under Cold Stress
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