The Capsicum baccatum-Specific Truncated NLR Protein CbCN Enhances the Innate Immunity against Colletotrichum acutatum
Chili pepper (Capsicum annuum) is an important fruit and spice used globally, but its yield is seriously threatened by anthracnose. Capsicum baccatum is particularly valuable as it carries advantageous disease resistance genes. However, most of the genes remain to be identified. In this study, we id...
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| Veröffentlicht in: | International journal of molecular sciences 2021-07, Vol.22 (14), p.7672 |
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| creator | Son, Seungmin Kim, Soohong Lee, Kyong Oh, Jun Choi, Inchan Do, Jae Yoon, Jae Han, Jungheon Park, Sang |
| description | Chili pepper (Capsicum annuum) is an important fruit and spice used globally, but its yield is seriously threatened by anthracnose. Capsicum baccatum is particularly valuable as it carries advantageous disease resistance genes. However, most of the genes remain to be identified. In this study, we identified the C. baccatum-specific gene CbCN, which encodes a truncated nucleotide-binding and leucine-rich repeat protein in the anthracnose resistant chili pepper variety PBC80. The transcription of CbCN was greater in PBC80 than it was in the susceptible variety An-S after Colletotrichum acutatum inoculation. In order to investigate the biological function of CbCN, we generated transgenic tobacco lines constitutively expressing CbCN. Notably, CbCN-overexpressing transgenic plants exhibited enhanced resistance to C. acutatum compared to wild-type plants. Moreover, the expression of pathogenesis-related (PR) genes was remarkably increased in a CbCN-overexpressing tobacco plants. In order to confirm these results in chili pepper, we silenced the CbCN gene using the virus-induced gene silencing system. The anthracnose resistance and expressions of PR1, PR2, and NPR1 were significantly reduced in CbCN-silenced chili peppers after C. acutatum inoculations. These results indicate that CbCN enhances the innate immunity against anthracnose caused by C. acutatum by regulating defense response genes. |
| doi_str_mv | 10.3390/ijms22147672 |
| format | Article |
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Capsicum baccatum is particularly valuable as it carries advantageous disease resistance genes. However, most of the genes remain to be identified. In this study, we identified the C. baccatum-specific gene CbCN, which encodes a truncated nucleotide-binding and leucine-rich repeat protein in the anthracnose resistant chili pepper variety PBC80. The transcription of CbCN was greater in PBC80 than it was in the susceptible variety An-S after Colletotrichum acutatum inoculation. In order to investigate the biological function of CbCN, we generated transgenic tobacco lines constitutively expressing CbCN. Notably, CbCN-overexpressing transgenic plants exhibited enhanced resistance to C. acutatum compared to wild-type plants. Moreover, the expression of pathogenesis-related (PR) genes was remarkably increased in a CbCN-overexpressing tobacco plants. In order to confirm these results in chili pepper, we silenced the CbCN gene using the virus-induced gene silencing system. The anthracnose resistance and expressions of PR1, PR2, and NPR1 were significantly reduced in CbCN-silenced chili peppers after C. acutatum inoculations. These results indicate that CbCN enhances the innate immunity against anthracnose caused by C. acutatum by regulating defense response genes.</description><identifier>ISSN: 1422-0067</identifier><identifier>ISSN: 1661-6596</identifier><identifier>EISSN: 1422-0067</identifier><identifier>DOI: 10.3390/ijms22147672</identifier><identifier>PMID: 34299290</identifier><language>eng</language><publisher>Basel: MDPI AG</publisher><subject>Anthracnose ; Apoptosis ; Binding sites ; Capsicum baccatum ; Chromosomes ; Cloning ; Colletotrichum acutatum ; Communication ; Comparative analysis ; Disease resistance ; Gene expression ; Gene silencing ; Genes ; Genomes ; Genomics ; Genotype & phenotype ; Innate immunity ; Inoculation ; Nlr protein ; Nucleotides ; Pathogens ; Peppers ; Peptides ; Proteins ; Quantitative analysis ; Software ; Tobacco ; Transcription ; Transgenic plants</subject><ispartof>International journal of molecular sciences, 2021-07, Vol.22 (14), p.7672</ispartof><rights>2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2021 by the authors. 2021</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c389t-5af26f6368122f8efdacaa63d0a8c3185f692d39e1a5cdaf469ba6e2ffa23aed3</citedby><cites>FETCH-LOGICAL-c389t-5af26f6368122f8efdacaa63d0a8c3185f692d39e1a5cdaf469ba6e2ffa23aed3</cites><orcidid>0000-0001-9770-8256 ; 0000-0003-4373-7929 ; 0000-0001-6977-6139</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8306327/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8306327/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,53766,53768</link.rule.ids></links><search><creatorcontrib>Son, Seungmin</creatorcontrib><creatorcontrib>Kim, Soohong</creatorcontrib><creatorcontrib>Lee, Kyong</creatorcontrib><creatorcontrib>Oh, Jun</creatorcontrib><creatorcontrib>Choi, Inchan</creatorcontrib><creatorcontrib>Do, Jae</creatorcontrib><creatorcontrib>Yoon, Jae</creatorcontrib><creatorcontrib>Han, Jungheon</creatorcontrib><creatorcontrib>Park, Sang</creatorcontrib><title>The Capsicum baccatum-Specific Truncated NLR Protein CbCN Enhances the Innate Immunity against Colletotrichum acutatum</title><title>International journal of molecular sciences</title><description>Chili pepper (Capsicum annuum) is an important fruit and spice used globally, but its yield is seriously threatened by anthracnose. Capsicum baccatum is particularly valuable as it carries advantageous disease resistance genes. However, most of the genes remain to be identified. In this study, we identified the C. baccatum-specific gene CbCN, which encodes a truncated nucleotide-binding and leucine-rich repeat protein in the anthracnose resistant chili pepper variety PBC80. The transcription of CbCN was greater in PBC80 than it was in the susceptible variety An-S after Colletotrichum acutatum inoculation. In order to investigate the biological function of CbCN, we generated transgenic tobacco lines constitutively expressing CbCN. Notably, CbCN-overexpressing transgenic plants exhibited enhanced resistance to C. acutatum compared to wild-type plants. Moreover, the expression of pathogenesis-related (PR) genes was remarkably increased in a CbCN-overexpressing tobacco plants. In order to confirm these results in chili pepper, we silenced the CbCN gene using the virus-induced gene silencing system. The anthracnose resistance and expressions of PR1, PR2, and NPR1 were significantly reduced in CbCN-silenced chili peppers after C. acutatum inoculations. These results indicate that CbCN enhances the innate immunity against anthracnose caused by C. acutatum by regulating defense response genes.</description><subject>Anthracnose</subject><subject>Apoptosis</subject><subject>Binding sites</subject><subject>Capsicum baccatum</subject><subject>Chromosomes</subject><subject>Cloning</subject><subject>Colletotrichum acutatum</subject><subject>Communication</subject><subject>Comparative analysis</subject><subject>Disease resistance</subject><subject>Gene expression</subject><subject>Gene silencing</subject><subject>Genes</subject><subject>Genomes</subject><subject>Genomics</subject><subject>Genotype & phenotype</subject><subject>Innate immunity</subject><subject>Inoculation</subject><subject>Nlr protein</subject><subject>Nucleotides</subject><subject>Pathogens</subject><subject>Peppers</subject><subject>Peptides</subject><subject>Proteins</subject><subject>Quantitative analysis</subject><subject>Software</subject><subject>Tobacco</subject><subject>Transcription</subject><subject>Transgenic plants</subject><issn>1422-0067</issn><issn>1661-6596</issn><issn>1422-0067</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>8G5</sourceid><sourceid>BENPR</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNpdkU1r3DAQhkVpaNK0t_4AQS891K0-LNm6FIpJm4UlCe32LGZlKavFlrb6COTfx0tCSHuaYebhfWd4EfpAyRfOFfnq93NmjLad7NgrdEZbxhpCZPf6RX-K3ua8J4RxJtQbdMpbphRT5AzdbXYWD3DI3tQZb8EYKHVufh-s8c4bvEk1LCM74qv1L3yTYrE-4GE7XOGLsINgbMZlkViFsFB4Nc81-HKP4RZ8yAUPcZpsiSV5s1sMwNRyNHiHThxM2b5_qufoz4-LzXDZrK9_robv68bwXpVGgGPSSS57ypjrrRvBAEg-EugNp71wUrGRK0tBmBFcK9UWpGXOAeNgR36Ovj3qHup2tqOxoSSY9CH5GdK9juD1v5vgd_o23umeE8lZtwh8ehJI8W-1uejZZ2OnCYKNNWsmhKC0FZ1c0I__oftYU1jeO1KtkEJJulCfHymTYs7JuudjKNHHQPXLQPkDrwqVLQ</recordid><startdate>20210718</startdate><enddate>20210718</enddate><creator>Son, Seungmin</creator><creator>Kim, Soohong</creator><creator>Lee, Kyong</creator><creator>Oh, Jun</creator><creator>Choi, Inchan</creator><creator>Do, Jae</creator><creator>Yoon, Jae</creator><creator>Han, Jungheon</creator><creator>Park, Sang</creator><general>MDPI AG</general><general>MDPI</general><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>MBDVC</scope><scope>PHGZM</scope><scope>PHGZT</scope><scope>PIMPY</scope><scope>PJZUB</scope><scope>PKEHL</scope><scope>PPXIY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0001-9770-8256</orcidid><orcidid>https://orcid.org/0000-0003-4373-7929</orcidid><orcidid>https://orcid.org/0000-0001-6977-6139</orcidid></search><sort><creationdate>20210718</creationdate><title>The Capsicum baccatum-Specific Truncated NLR Protein CbCN Enhances the Innate Immunity against Colletotrichum acutatum</title><author>Son, Seungmin ; 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Capsicum baccatum is particularly valuable as it carries advantageous disease resistance genes. However, most of the genes remain to be identified. In this study, we identified the C. baccatum-specific gene CbCN, which encodes a truncated nucleotide-binding and leucine-rich repeat protein in the anthracnose resistant chili pepper variety PBC80. The transcription of CbCN was greater in PBC80 than it was in the susceptible variety An-S after Colletotrichum acutatum inoculation. In order to investigate the biological function of CbCN, we generated transgenic tobacco lines constitutively expressing CbCN. Notably, CbCN-overexpressing transgenic plants exhibited enhanced resistance to C. acutatum compared to wild-type plants. Moreover, the expression of pathogenesis-related (PR) genes was remarkably increased in a CbCN-overexpressing tobacco plants. In order to confirm these results in chili pepper, we silenced the CbCN gene using the virus-induced gene silencing system. The anthracnose resistance and expressions of PR1, PR2, and NPR1 were significantly reduced in CbCN-silenced chili peppers after C. acutatum inoculations. These results indicate that CbCN enhances the innate immunity against anthracnose caused by C. acutatum by regulating defense response genes.</abstract><cop>Basel</cop><pub>MDPI AG</pub><pmid>34299290</pmid><doi>10.3390/ijms22147672</doi><orcidid>https://orcid.org/0000-0001-9770-8256</orcidid><orcidid>https://orcid.org/0000-0003-4373-7929</orcidid><orcidid>https://orcid.org/0000-0001-6977-6139</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Anthracnose Apoptosis Binding sites Capsicum baccatum Chromosomes Cloning Colletotrichum acutatum Communication Comparative analysis Disease resistance Gene expression Gene silencing Genes Genomes Genomics Genotype & phenotype Innate immunity Inoculation Nlr protein Nucleotides Pathogens Peppers Peptides Proteins Quantitative analysis Software Tobacco Transcription Transgenic plants |
| title | The Capsicum baccatum-Specific Truncated NLR Protein CbCN Enhances the Innate Immunity against Colletotrichum acutatum |
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